SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30949
         (687 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||...   429   e-121
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb...   242   3e-65
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s...   235   4e-63
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce...   131   1e-31
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po...   109   3e-25
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc...    98   1e-21
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy...    69   6e-13
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe...    58   9e-10
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa...    46   5e-06
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb...    34   0.022
SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha...    27   2.5  
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom...    27   3.4  
SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosacc...    25   7.8  

>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 375

 Score =  429 bits (1056), Expect = e-121
 Identities = 203/228 (89%), Positives = 216/228 (94%)
 Frame = +2

Query: 2   FETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGR 181
           FETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIYEGYALPHAI+RLDLAGR
Sbjct: 124 FETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRLDLAGR 183

Query: 182 DLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELP 361
           DLTDYLMKIL ERGY+F+TTAEREIVRDIKEKLCYVALDFEQE+ TAA S+SLEKSYELP
Sbjct: 184 DLTDYLMKILMERGYTFSTTAEREIVRDIKEKLCYVALDFEQELQTAAQSSSLEKSYELP 243

Query: 362 DGQVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGT 541
           DGQVITIGNERFR PEALFQPS LG+E+ GIHE  YNSIMKCDVDIRKDLY N VMSGGT
Sbjct: 244 DGQVITIGNERFRAPEALFQPSALGLENAGIHEATYNSIMKCDVDIRKDLYGNVVMSGGT 303

Query: 542 TMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLST 685
           TMYPGIADRMQKEI ALAPS++K+KI+APPERKYSVWIGGSILASLST
Sbjct: 304 TMYPGIADRMQKEIQALAPSSMKVKIVAPPERKYSVWIGGSILASLST 351


>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 379

 Score =  242 bits (593), Expect = 3e-65
 Identities = 110/228 (48%), Positives = 159/228 (69%)
 Frame = +2

Query: 2   FETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGR 181
           +ET N PA+  ++Q VL+LYAS RTTGIVL+ GDG++H+VPIY+G+++P AI + ++ GR
Sbjct: 129 YETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAIQQEEIGGR 188

Query: 182 DLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELP 361
           D+TDYL   L + G+   ++AE+EIVR+IKEK CYVA DF  E+ +         +Y+LP
Sbjct: 189 DVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYVASDFRSEIESWTEHKPQIHTYQLP 248

Query: 362 DGQVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGT 541
           D Q IT+G E F  PE LF P  +G E+ G+H  ++ SI+  D+D+R  LY+N V+SGG+
Sbjct: 249 DNQTITLGTECFSAPEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSNIVLSGGS 308

Query: 542 TMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLST 685
           T+  G  +R   E+ A++    ++KI A PER ++ W+GGSILASLST
Sbjct: 309 TLLRGFGERFISELRAISGKKNQVKIYASPERMHNAWLGGSILASLST 356


>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
           subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 390

 Score =  235 bits (575), Expect = 4e-63
 Identities = 114/237 (48%), Positives = 163/237 (68%), Gaps = 11/237 (4%)
 Frame = +2

Query: 2   FETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGR 181
           FE +    +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE   L H + RLD+AGR
Sbjct: 124 FERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVGRLDVAGR 183

Query: 182 DLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELP 361
           D T YL+ +L  +GY+F  TA+ E VR++KEKLCYV+ D E +   +  +T L ++Y LP
Sbjct: 184 DATRYLISLLLRKGYAFNRTADFETVREMKEKLCYVSYDLELDHKLSEETTVLMRNYTLP 243

Query: 362 DGQVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGT 541
           DG+VI +G+ER+ CPE LFQP  +G E  G+ E ++++I   DVDIRK LY   V+SGG+
Sbjct: 244 DGRVIKVGSERYECPECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLYRAIVLSGGS 303

Query: 542 TMYPGIADRMQKEITAL--------APSTI---KIKIIAPPERKYSVWIGGSILASL 679
           +MY G+  R++KEI  L         P+ +   K+KI   P R+++V+IGG++LA +
Sbjct: 304 SMYAGLPSRLEKEIKQLWFERVLHGDPARLPNFKVKIEDAPRRRHAVFIGGAVLADI 360


>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 427

 Score =  131 bits (316), Expect = 1e-31
 Identities = 86/253 (33%), Positives = 132/253 (52%), Gaps = 29/253 (11%)
 Frame = +2

Query: 2   FETFNSPAMYVAIQAVLSLYASGRT--------TGIVLDSGDGVSHTVPIYEGYALPHAI 157
           FE+FN   +Y+A+QAVL+L AS  +        TG V+DSGDGV+H +P+ EGY +  +I
Sbjct: 147 FESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIPVAEGYVIGSSI 206

Query: 158 LRLDLAGRDLTDYLMKILTERGY--SFTTTAEREIVRDIKEKLCYVALDFEQEMA--TAA 325
             + LAGRD+T ++  +L +R    S   TAER     IKE+ CYV  D  +E +     
Sbjct: 207 KTMPLAGRDVTYFVQSLLRDRNEPDSSLKTAER-----IKEECCYVCPDIVKEFSRFDRE 261

Query: 326 ASTSLEKSYELPDGQ--VITIGNERFRCPEALFQPSFLGME-SCGIHETVYNSIMKCDVD 496
               L+ + E   G    I +G ERF  PE  F P     +    + E V N +    +D
Sbjct: 262 PDRYLKYASESITGHSTTIDVGFERFLAPEIFFNPEIASSDFLTPLPELVDNVVQSSPID 321

Query: 497 IRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTI--------------KIKIIAPPE 634
           +RK LY N V+SGG+T++    +R+Q+++  +    I               + +I+   
Sbjct: 322 VRKGLYKNIVLSGGSTLFKNFGNRLQRDLKRIVDERIHRSEMLSGAKSGGVDVNVISHKR 381

Query: 635 RKYSVWIGGSILA 673
           ++ +VW GGS+LA
Sbjct: 382 QRNAVWFGGSLLA 394


>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 401

 Score =  109 bits (263), Expect = 3e-25
 Identities = 65/204 (31%), Positives = 105/204 (51%), Gaps = 20/204 (9%)
 Frame = +2

Query: 83  IVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVR 262
           +V+DSG   +H +P+ +      A+ R+D+ G+ LT+YL ++++ R Y+     E  IV 
Sbjct: 159 LVIDSGYSFTHIIPVIDFSVQEQAVKRVDVGGKLLTNYLKEVISYRKYNMME--ETYIVN 216

Query: 263 DIKEKLCYVALDFEQEMATAAAS--TSLEKSYELPD------------------GQVITI 382
           +IKE +CYV+ +F+++M        + LE  Y LPD                   QV+ +
Sbjct: 217 EIKESVCYVSQNFKEDMEICHEKPRSKLEICYALPDYSTGKHGYIVRDINQKIEQQVLNL 276

Query: 383 GNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIA 562
            NERF  PE LF PS + +   GI E V  S+     +I+  L  N V  GG   +PG  
Sbjct: 277 SNERFMIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCKFPGFH 336

Query: 563 DRMQKEITALAPSTIKIKIIAPPE 634
            R+  E+ +LAP+  ++K+  P +
Sbjct: 337 KRLSSELRSLAPANWEVKVFEPSD 360


>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
           Arp42|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 430

 Score = 97.9 bits (233), Expect = 1e-21
 Identities = 60/162 (37%), Positives = 84/162 (51%), Gaps = 11/162 (6%)
 Frame = +2

Query: 227 SFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCP 406
           S+    +R ++ + KE +  V    +     A AS+   K +E PDG     G ERFR  
Sbjct: 243 SYEIFQKRRVIEEWKESVLDV---LDTPFDEAKASSRNPKPFEFPDGVTHKFGQERFRIS 299

Query: 407 EALFQPSFLGMESC---------GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGI 559
           E LF PSF    S          G+HE VY SI+ CD ++R  L  N V++GGT++ PG+
Sbjct: 300 EILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSELRSPLLNNIVVTGGTSLIPGL 359

Query: 560 ADRMQKEITALAP-STIKIKII-APPERKYSVWIGGSILASL 679
           ++R+Q E+  LA  S I +           +VW GGSILASL
Sbjct: 360 SERLQAEVQRLATGSRINVHTAETASATSNAVWFGGSILASL 401


>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 433

 Score = 68.9 bits (161), Expect = 6e-13
 Identities = 36/82 (43%), Positives = 52/82 (63%), Gaps = 3/82 (3%)
 Frame = +2

Query: 449 GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAP 628
           GI +   N I +CDVDIR  L  N ++ GGT++  G + R+Q E++ L P + ++KI A 
Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHAS 384

Query: 629 ---PERKYSVWIGGSILASLST 685
               ER Y+ W+GGSIL+SL T
Sbjct: 385 GHVVERSYASWLGGSILSSLGT 406



 Score = 44.0 bits (99), Expect = 2e-05
 Identities = 22/75 (29%), Positives = 37/75 (49%)
 Frame = +2

Query: 2   FETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGR 181
           FE+   PA Y+A Q   + +ASG+ T  ++D G   S    IY+G+ L         +G 
Sbjct: 122 FESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVLQKGYQVQHFSGN 181

Query: 182 DLTDYLMKILTERGY 226
            + D L + L ++ +
Sbjct: 182 AINDILAQTLRDKNF 196


>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 721

 Score = 58.4 bits (135), Expect = 9e-10
 Identities = 25/92 (27%), Positives = 50/92 (54%)
 Frame = +2

Query: 389 ERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADR 568
           E+ R PE +F PS +G++  GI E + + + +  ++ ++ L +N +++GG    PG+  R
Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653

Query: 569 MQKEITALAPSTIKIKIIAPPERKYSVWIGGS 664
           +++E+T++ P    I +          W G S
Sbjct: 654 IKRELTSIMPVGSSINVFRASNPLLDAWKGAS 685



 Score = 53.2 bits (122), Expect = 3e-08
 Identities = 30/117 (25%), Positives = 56/117 (47%), Gaps = 2/117 (1%)
 Frame = +2

Query: 2   FETFNSPAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLA 175
           FE +N+P++   I  + S Y + +  ++GIVL+ G+  SH +P+  G  +     R+   
Sbjct: 139 FELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVIPVLNGERILSEAKRISWG 198

Query: 176 GRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEK 346
           G   + YL+K+   +  SF           +    C+V+ D+  ++A A     LE+
Sbjct: 199 GSQSSSYLLKLFQIKYPSFPIKMLPSQAELLMHDHCHVSSDYTHDIAHALDRDILER 255


>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
           Arp9|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 523

 Score = 46.0 bits (104), Expect = 5e-06
 Identities = 27/91 (29%), Positives = 44/91 (48%)
 Frame = +2

Query: 2   FETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGR 181
           FE    P   +A + ++ LYA G   G+V+D G   +   PI +G  +  A  +L L GR
Sbjct: 170 FEECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLGGR 229

Query: 182 DLTDYLMKILTERGYSFTTTAEREIVRDIKE 274
            +T +L  +L E   +  ++ +     DI E
Sbjct: 230 YMTQHLQNLLRESLPTLKSSGQYVSKEDITE 260


>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 620

 Score = 33.9 bits (74), Expect = 0.022
 Identities = 16/58 (27%), Positives = 31/58 (53%), Gaps = 3/58 (5%)
 Frame = +2

Query: 503 KDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPP---ERKYSVWIGGSI 667
           K+LY++ ++ GG   +PG A  +++ I +   +   I +I PP   + ++  W G  I
Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACI 584



 Score = 31.5 bits (68), Expect = 0.12
 Identities = 13/64 (20%), Positives = 34/64 (53%)
 Frame = +2

Query: 41  QAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTER 220
           +++ + + +G +   V+D G   +    + EG  +P++ ++++  G D+T   MK+L   
Sbjct: 280 ESLCTSFGAGMSAACVVDMGAQKTSISCVEEGVVVPNSRIKINYGGDDITLLFMKLLMRS 339

Query: 221 GYSF 232
            + +
Sbjct: 340 HFPY 343


>SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 617

 Score = 27.1 bits (57), Expect = 2.5
 Identities = 14/37 (37%), Positives = 20/37 (54%)
 Frame = +2

Query: 575 KEITALAPSTIKIKIIAPPERKYSVWIGGSILASLST 685
           KEI++ A S I +  + P E+    W    ILA L+T
Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248


>SPAC1296.04 |mug65||spore wall assembly protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 248

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 9/16 (56%), Positives = 9/16 (56%)
 Frame = +3

Query: 126 STKVTLCPTPSSVWTW 173
           STK   CP PS  W W
Sbjct: 52  STKTATCPDPSWEWAW 67


>SPBC16A3.08c |||nuclear telomere cap complex subunit
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 284

 Score = 25.4 bits (53), Expect = 7.8
 Identities = 15/43 (34%), Positives = 20/43 (46%)
 Frame = +1

Query: 454 PRDRVQLHHEVRRRHP*GPVRQHRHVRWYHHVPRYRRQDAEGD 582
           PR+ V      R+R P  P  + R V      PR RRQ  +G+
Sbjct: 41  PRELVAQSTTSRKRDPNQPTPRERTVNKKADQPRRRRQAPQGN 83


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,438,425
Number of Sequences: 5004
Number of extensions: 46537
Number of successful extensions: 193
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 171
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 184
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 317927284
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -