BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30923 (671 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC947.07 |||ribosome biogenesis protein Rrp14-C|Schizosaccharo... 34 0.021 SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 31 0.15 SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|ch... 30 0.26 SPAC22G7.05 |||krr family protein|Schizosaccharomyces pombe|chr ... 28 1.1 SPAC1556.06.1 |meu1|SPAC1556.06a, SPAC1556.06|sequence orphan|Sc... 27 1.9 SPAC1834.07 |klp3|krp1|kinesin-like protein Klp3|Schizosaccharom... 27 2.5 SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 27 3.3 SPAC22F3.11c |snu23||U4/U6 x U5 tri-snRNP complex subunit Snu23|... 26 5.7 SPBC2A9.07c |||zf-PARP-type zinc finger protein|Schizosaccharomy... 26 5.7 SPBPB8B6.02c |||urea transporter |Schizosaccharomyces pombe|chr ... 26 5.7 SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomy... 25 7.5 SPAC6F6.12 |||autophagy associated protein Atg24|Schizosaccharom... 25 9.9 SPAC1B3.05 |||CCR4-Not complex subunit Not3/5 |Schizosaccharomyc... 25 9.9 SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein homolog|Schizosacch... 25 9.9 SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 25 9.9 SPCC4B3.08 |||C-terminal domain kinase I |Schizosaccharomyces po... 25 9.9 >SPBC947.07 |||ribosome biogenesis protein Rrp14-C|Schizosaccharomyces pombe|chr 2|||Manual Length = 233 Score = 33.9 bits (74), Expect = 0.021 Identities = 21/64 (32%), Positives = 34/64 (53%) Frame = +2 Query: 443 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEK 622 EEKR+++EE++K + +LQA + K N + KKS + + KE+ + EK Sbjct: 138 EEKRRKIEESDKWHRVLLQA--EGKKLKDNEQLLKKSIRRKEKEKKKSSDAWKERKDNEK 195 Query: 623 KISL 634 K L Sbjct: 196 KAML 199 >SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizosaccharomyces pombe|chr 1|||Manual Length = 1403 Score = 31.1 bits (67), Expect = 0.15 Identities = 22/77 (28%), Positives = 38/77 (49%), Gaps = 1/77 (1%) Frame = +2 Query: 437 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLE-RNKTKEQLE 613 + EEK +R E + KR+A +A ++A + ++K++ NA+ E K K + E Sbjct: 620 EAEEKAKREAEEKAKREAEEKAKREAEEKAKR-EAEEKAKREAEENAKREAEEKAKREAE 678 Query: 614 EEKKISLSIRIKPLTIE 664 E K ++K T E Sbjct: 679 ENAKREAEEKVKRETEE 695 Score = 29.5 bits (63), Expect = 0.46 Identities = 19/64 (29%), Positives = 33/64 (51%), Gaps = 1/64 (1%) Frame = +2 Query: 437 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLE-RNKTKEQLE 613 + EEK +R E + KR+A +A ++A + ++K++ NA+ E K K + E Sbjct: 636 EAEEKAKREAEEKAKREAEEKAKREAEENAKR-EAEEKAKREAEENAKREAEEKVKRETE 694 Query: 614 EEKK 625 E K Sbjct: 695 ENAK 698 Score = 27.5 bits (58), Expect = 1.9 Identities = 18/64 (28%), Positives = 33/64 (51%), Gaps = 1/64 (1%) Frame = +2 Query: 437 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLE-RNKTKEQLE 613 + EEK +R E + KR+A +A ++A + ++K++ A+ E K K + E Sbjct: 572 EAEEKAKREAEEKAKREAEEKAKREAEENAKR-EAEEKAKREAEEKAKREAEEKAKREAE 630 Query: 614 EEKK 625 E+ K Sbjct: 631 EKAK 634 Score = 27.5 bits (58), Expect = 1.9 Identities = 18/64 (28%), Positives = 32/64 (50%), Gaps = 1/64 (1%) Frame = +2 Query: 437 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQK-KSENFGLSNAQLERNKTKEQLE 613 + EEK +R E + KR+A +A ++A + +K K E + + E N +E E Sbjct: 612 EAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEENAKREAEE 671 Query: 614 EEKK 625 + K+ Sbjct: 672 KAKR 675 Score = 26.6 bits (56), Expect = 3.3 Identities = 18/64 (28%), Positives = 32/64 (50%), Gaps = 1/64 (1%) Frame = +2 Query: 437 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLE-RNKTKEQLE 613 + EEK +R E + KR+A A ++A + ++K++ A+ E K K + E Sbjct: 580 EAEEKAKREAEEKAKREAEENAKREAEEKAKR-EAEEKAKREAEEKAKREAEEKAKREAE 638 Query: 614 EEKK 625 E+ K Sbjct: 639 EKAK 642 Score = 26.6 bits (56), Expect = 3.3 Identities = 18/64 (28%), Positives = 32/64 (50%), Gaps = 1/64 (1%) Frame = +2 Query: 437 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLE-RNKTKEQLE 613 + EEK +R E KR+A +A ++A + ++K++ A+ E K K + E Sbjct: 588 EAEEKAKREAEENAKREAEEKAKREAEEKAKR-EAEEKAKREAEEKAKREAEEKAKREAE 646 Query: 614 EEKK 625 E+ K Sbjct: 647 EKAK 650 Score = 26.6 bits (56), Expect = 3.3 Identities = 18/64 (28%), Positives = 32/64 (50%), Gaps = 1/64 (1%) Frame = +2 Query: 437 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLE-RNKTKEQLE 613 + EEK +R E + KR+A +A ++A + ++K++ A+ E K K + E Sbjct: 604 EAEEKAKREAEEKAKREAEEKAKREAEEKAKR-EAEEKAKREAEEKAKREAEEKAKREAE 662 Query: 614 EEKK 625 E K Sbjct: 663 ENAK 666 Score = 25.8 bits (54), Expect = 5.7 Identities = 19/62 (30%), Positives = 31/62 (50%), Gaps = 2/62 (3%) Frame = +2 Query: 446 EKRQRLEEAEK-KRQAMLQAMKDASKTGPNFTIQK-KSENFGLSNAQLERNKTKEQLEEE 619 E++ RLE E KR+A QA ++A + +K K E + + E N +E E+ Sbjct: 550 EEKARLEAEENAKREAEEQAKREAEEKAKREAEEKAKREAEEKAKREAEENAKREAEEKA 609 Query: 620 KK 625 K+ Sbjct: 610 KR 611 >SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 750 Score = 30.3 bits (65), Expect = 0.26 Identities = 21/78 (26%), Positives = 38/78 (48%), Gaps = 4/78 (5%) Frame = +2 Query: 437 DIEEKRQRLEEAEKKRQAMLQAMKD----ASKTGPNFTIQKKSENFGLSNAQLERNKTKE 604 ++EEK LE A+ Q ++ ++KD +K + ++ G+S+A L + K K Sbjct: 162 ELEEKINSLESAQSIEQEVISSLKDDKTVETKNDVPEVSRPSTDTIGVSSA-LSKKKKKR 220 Query: 605 QLEEEKKISLSIRIKPLT 658 + +KK S I+ T Sbjct: 221 NRKNQKKKSTKQNIEATT 238 >SPAC22G7.05 |||krr family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 598 Score = 28.3 bits (60), Expect = 1.1 Identities = 17/54 (31%), Positives = 31/54 (57%) Frame = +2 Query: 461 LEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEK 622 +EE E+ R+ + A D + G + +S+ G + +++ KTK+QLEEE+ Sbjct: 197 VEEQERLRKETIAAFHDVN--GNKDAVSNESDEDG--DFLVKKEKTKKQLEEEE 246 >SPAC1556.06.1 |meu1|SPAC1556.06a, SPAC1556.06|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 776 Score = 27.5 bits (58), Expect = 1.9 Identities = 18/60 (30%), Positives = 32/60 (53%), Gaps = 3/60 (5%) Frame = +2 Query: 449 KRQRLEEAEKKRQAMLQAMKDAS---KTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEE 619 K + +++R A +QA+ D S +T + + +SEN GL N ++ +QLE+E Sbjct: 232 KDHEILRLKEERTAAMQAIDDISGTIETIKSDCYKVESENKGLINEVMDMRNFVQQLEQE 291 >SPAC1834.07 |klp3|krp1|kinesin-like protein Klp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 554 Score = 27.1 bits (57), Expect = 2.5 Identities = 14/49 (28%), Positives = 28/49 (57%), Gaps = 1/49 (2%) Frame = +2 Query: 527 PNFTIQKKSENFGLSNAQLERNKTKEQLEE-EKKISLSIRIKPLTIEGL 670 P FTI++K +NF ++N ERN ++L + ++ + ++ I+ L Sbjct: 490 PGFTIEQKDKNFSINN---ERNNFLQKLSTLDSSLAALVNVQRKLIKAL 535 >SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1336 Score = 26.6 bits (56), Expect = 3.3 Identities = 13/39 (33%), Positives = 22/39 (56%) Frame = -2 Query: 289 PLFAPFVDVFLQLFIQVRPLLVLTLDEFWISLTLLFRSG 173 P+FA + L LF+Q+ P + + FW S+ L+ +G Sbjct: 796 PIFAYVISKCLNLFMQIDPSIGVA---FWSSMVLVVAAG 831 >SPAC22F3.11c |snu23||U4/U6 x U5 tri-snRNP complex subunit Snu23|Schizosaccharomyces pombe|chr 1|||Manual Length = 151 Score = 25.8 bits (54), Expect = 5.7 Identities = 15/69 (21%), Positives = 36/69 (52%) Frame = +2 Query: 440 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEE 619 I EKR LEE +++ + + + + K ++++++ E + + +LE K + + ++ Sbjct: 65 IIEKRATLEEVKERMEYWRRQLLEPEKGSEEYSLKERVERY---HQELEAKKLRRKQKKV 121 Query: 620 KKISLSIRI 646 K S R+ Sbjct: 122 NKEKNSPRL 130 >SPBC2A9.07c |||zf-PARP-type zinc finger protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 274 Score = 25.8 bits (54), Expect = 5.7 Identities = 16/64 (25%), Positives = 35/64 (54%), Gaps = 2/64 (3%) Frame = +2 Query: 443 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTK--EQLEE 616 EEK +++EE E + + ++D K+ +++K S A+ +R+ + E LE+ Sbjct: 132 EEKDRKIEEGELTSEEEKEPIQDLRKSHKRKSVEKSSVPNKKHKAERKRSPSPKIEILED 191 Query: 617 EKKI 628 +++I Sbjct: 192 DEEI 195 >SPBPB8B6.02c |||urea transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 673 Score = 25.8 bits (54), Expect = 5.7 Identities = 14/34 (41%), Positives = 17/34 (50%) Frame = -1 Query: 104 CWLFIECRTGRSRSLSHSALVLGPAAQRRWNVES 3 CW I G S L+ AL+L PA+ NV S Sbjct: 292 CWWIIPMALGSSAGLACRALLLNPASVTYPNVLS 325 >SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 543 Score = 25.4 bits (53), Expect = 7.5 Identities = 8/24 (33%), Positives = 14/24 (58%) Frame = -3 Query: 555 SLFFWMVKLGPVLLASFMAWSIAW 484 SL+FW L P +F++W + + Sbjct: 111 SLYFWTAYLSPPKYRAFLSWFLGY 134 >SPAC6F6.12 |||autophagy associated protein Atg24|Schizosaccharomyces pombe|chr 1|||Manual Length = 401 Score = 25.0 bits (52), Expect = 9.9 Identities = 11/20 (55%), Positives = 16/20 (80%) Frame = +2 Query: 206 EFIKRQDQKRSDLDEQLKEY 265 E +KR+DQK+ D+ E L+EY Sbjct: 272 ELLKRRDQKQQDV-EALQEY 290 >SPAC1B3.05 |||CCR4-Not complex subunit Not3/5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 630 Score = 25.0 bits (52), Expect = 9.9 Identities = 21/75 (28%), Positives = 37/75 (49%), Gaps = 1/75 (1%) Frame = +2 Query: 449 KRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKE-QLEEEKK 625 ++++LE K + LQ ++D KT + S + A LE + E ++EE K Sbjct: 29 QKEKLEGDLKTQIKKLQRLRDQIKTWAS------SNDIKDKKALLENRRLIEAKMEEFKA 82 Query: 626 ISLSIRIKPLTIEGL 670 + ++IK + EGL Sbjct: 83 VEREMKIKAFSKEGL 97 >SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 2609 Score = 25.0 bits (52), Expect = 9.9 Identities = 7/24 (29%), Positives = 13/24 (54%) Frame = -2 Query: 97 CLLSVARVEVGHSVTRHWFLDRQH 26 C L + + +++ R W L R+H Sbjct: 80 CCLQILGIATSYTILRSWLLSRKH 103 >SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccharomyces pombe|chr 2|||Manual Length = 884 Score = 25.0 bits (52), Expect = 9.9 Identities = 19/66 (28%), Positives = 34/66 (51%) Frame = +2 Query: 443 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEK 622 +E+ ++LEE + + L+ ++ + KT QKK+ L E + KE E +K Sbjct: 608 QEEMRKLEEKIESTKYGLETIQTSGKTIKQRIEQKKTRLMILREELQELDAIKE--EAQK 665 Query: 623 KISLSI 640 +IS S+ Sbjct: 666 QISQSL 671 >SPCC4B3.08 |||C-terminal domain kinase I |Schizosaccharomyces pombe|chr 3|||Manual Length = 218 Score = 25.0 bits (52), Expect = 9.9 Identities = 18/64 (28%), Positives = 29/64 (45%) Frame = +2 Query: 440 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEE 619 + EK+ + K A ++A + ASK+G T S+N L + +R + K E Sbjct: 116 LHEKKVIDDNQYKDAMATVEAHEQASKSGDTSTSGAISKNDILKRIEEDRERHKRMRENI 175 Query: 620 KKIS 631 IS Sbjct: 176 WAIS 179 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,807,379 Number of Sequences: 5004 Number of extensions: 24866 Number of successful extensions: 146 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 130 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 144 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 307866294 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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