Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30919
(304 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g56630.1 68418.m07070 phosphofructokinase family protein simi... 37 0.002
At4g32840.1 68417.m04670 phosphofructokinase family protein simi... 37 0.002
At4g26270.1 68417.m03780 phosphofructokinase family protein simi... 37 0.003
At4g29220.1 68417.m04180 phosphofructokinase family protein simi... 36 0.007
At5g61580.1 68418.m07727 phosphofructokinase family protein simi... 29 0.79
At2g22480.1 68415.m02667 phosphofructokinase family protein simi... 29 0.79
At4g21640.1 68417.m03136 subtilase family protein similar to sub... 28 1.0
At5g43400.1 68418.m05305 expressed protein strong similarity to ... 27 2.4
At2g43400.1 68415.m05394 electron transfer flavoprotein-ubiquino... 27 2.4
At5g47810.1 68418.m05905 phosphofructokinase family protein simi... 27 3.2
At2g32900.1 68415.m04033 centromere/kinetochore protein, putativ... 27 3.2
At2g21370.2 68415.m02542 xylulose kinase, putative similar to xy... 27 3.2
At2g21370.1 68415.m02543 xylulose kinase, putative similar to xy... 27 3.2
At5g42960.1 68418.m05239 expressed protein 26 5.6
At5g14770.1 68418.m01733 pentatricopeptide (PPR) repeat-containi... 25 7.4
At5g04750.1 68418.m00488 F1F0-ATPase inhibitor protein, putative... 25 9.7
At4g36090.1 68417.m05137 oxidoreductase, 2OG-Fe(II) oxygenase fa... 25 9.7
At2g03420.1 68415.m00300 expressed protein 25 9.7
At1g07220.1 68414.m00768 expressed protein 25 9.7
>At5g56630.1 68418.m07070 phosphofructokinase family protein similar
to phosphofructokinase [Amycolatopsis methanolica]
GI:17432243; contains Pfam profile PF00365:
Phosphofructokinase
Length = 485
Score = 37.1 bits (82), Expect = 0.002
Identities = 24/62 (38%), Positives = 35/62 (56%), Gaps = 1/62 (1%)
Frame = +2
Query: 41 SSVSSIIHK-GGTIIGSARCMEFIKKERRFKAAYNLMSRGITNLAVIGGDGSLTGANLFR 217
S V + IHK GGTIIG++R K ++ RGI + +IGGDG+ GA++
Sbjct: 146 SKVVNDIHKRGGTIIGTSRGGHDTNK-----IVDSIQDRGINQVYIIGGDGTQRGASVIF 200
Query: 218 QE 223
+E
Sbjct: 201 EE 202
>At4g32840.1 68417.m04670 phosphofructokinase family protein similar
to phosphofructokinase [Amycolatopsis methanolica]
GI:17432243; contains Pfam profile PF00365:
Phosphofructokinase
Length = 462
Score = 37.1 bits (82), Expect = 0.002
Identities = 22/60 (36%), Positives = 32/60 (53%)
Frame = +2
Query: 44 SVSSIIHKGGTIIGSARCMEFIKKERRFKAAYNLMSRGITNLAVIGGDGSLTGANLFRQE 223
+VS I +GGTI+G++R K N+ R I + +IGGDG+ GAN +E
Sbjct: 149 TVSDIHKRGGTILGTSR-----GGHDTSKIVDNIQDREINQVYIIGGDGTQKGANAIYKE 203
>At4g26270.1 68417.m03780 phosphofructokinase family protein similar
to phosphofructokinase [Amycolatopsis methanolica]
GI:17432243; contains Pfam profile PF00365:
Phosphofructokinase
Length = 489
Score = 36.7 bits (81), Expect = 0.003
Identities = 23/62 (37%), Positives = 35/62 (56%), Gaps = 1/62 (1%)
Frame = +2
Query: 41 SSVSSIIHK-GGTIIGSARCMEFIKKERRFKAAYNLMSRGITNLAVIGGDGSLTGANLFR 217
S V + IHK GGTI+G++R K ++ RGI + +IGGDG+ GA++
Sbjct: 146 SKVVNDIHKRGGTILGTSR-----GGHDTTKIVDSIQDRGINQVYIIGGDGTQRGASVIF 200
Query: 218 QE 223
+E
Sbjct: 201 EE 202
>At4g29220.1 68417.m04180 phosphofructokinase family protein similar
to phosphofructokinase [Amycolatopsis methanolica]
GI:17432243; contains Pfam profile PF00365:
Phosphofructokinase
Length = 473
Score = 35.5 bits (78), Expect = 0.007
Identities = 21/60 (35%), Positives = 31/60 (51%)
Frame = +2
Query: 44 SVSSIIHKGGTIIGSARCMEFIKKERRFKAAYNLMSRGITNLAVIGGDGSLTGANLFRQE 223
+V+ I GGTI+G++R K ++ RGI + +IGGDGS GA +E
Sbjct: 149 TVNDIHRSGGTILGTSR-----GGHNTTKIVDSIQDRGINQVYIIGGDGSQKGAAAIFEE 203
>At5g61580.1 68418.m07727 phosphofructokinase family protein similar
to phosphofructokinase [Amycolatopsis methanolica]
GI:17432243; contains Pfam profile PF00365:
Phosphofructokinase
Length = 530
Score = 28.7 bits (61), Expect = 0.79
Identities = 18/59 (30%), Positives = 28/59 (47%)
Frame = +2
Query: 47 VSSIIHKGGTIIGSARCMEFIKKERRFKAAYNLMSRGITNLAVIGGDGSLTGANLFRQE 223
V+ I +GGT + ++R K N+ RGI + +IGG G+ GA +E
Sbjct: 200 VNDIHKRGGTFLQTSR-----GGHDTAKIVDNIQDRGINQVYIIGGGGTQKGAEKIYEE 253
>At2g22480.1 68415.m02667 phosphofructokinase family protein similar
to phosphofructokinase [Amycolatopsis methanolica]
GI:17432243; contains Pfam profile PF00365:
Phosphofructokinase
Length = 537
Score = 28.7 bits (61), Expect = 0.79
Identities = 18/59 (30%), Positives = 30/59 (50%)
Frame = +2
Query: 47 VSSIIHKGGTIIGSARCMEFIKKERRFKAAYNLMSRGITNLAVIGGDGSLTGANLFRQE 223
V +I GG+++G +R + + ++ RGI L V+GG+G+ GAN E
Sbjct: 238 VQNIHLSGGSLLGVSRGGPSVSE-----IVDSMEERGINMLFVLGGNGTHAGANAIHNE 291
>At4g21640.1 68417.m03136 subtilase family protein similar to
subtilase SP1 [Oryza sativa] GI:9957714
Length = 733
Score = 28.3 bits (60), Expect = 1.0
Identities = 18/66 (27%), Positives = 29/66 (43%), Gaps = 2/66 (3%)
Frame = +2
Query: 2 VVWQVITLKEANWSSVSSIIHKGGTIIGSARCMEFIKKERR--FKAAYNLMSRGITNLAV 175
V W I W + IH ++ + + R F AA++ +++GIT +A
Sbjct: 286 VGWGGICSSADMWKAYDDAIHDQVDVLSVSIGASIPEDSERVDFIAAFHAVAKGITVVAA 345
Query: 176 IGGDGS 193
G DGS
Sbjct: 346 AGNDGS 351
>At5g43400.1 68418.m05305 expressed protein strong similarity to
unknown protein (emb|CAB86628.1)
Length = 655
Score = 27.1 bits (57), Expect = 2.4
Identities = 15/56 (26%), Positives = 29/56 (51%)
Frame = +2
Query: 98 MEFIKKERRFKAAYNLMSRGITNLAVIGGDGSLTGANLFRQEWSNLLDELLENNKI 265
+E +K ++ AA L+ I N + GS GA + +W+ ++D+L + K+
Sbjct: 376 LEDVKSGKKKIAAGALLPHQIIN-QLEDDSGSEVGAEVAELQWARMVDDLAKKGKL 430
>At2g43400.1 68415.m05394 electron transfer flavoprotein-ubiquinone
oxidoreductase family protein contains Pfam profile:
PF05187 Electron transfer flavoprotein-ubiquinone
oxidoreductase
Length = 633
Score = 27.1 bits (57), Expect = 2.4
Identities = 21/74 (28%), Positives = 28/74 (37%)
Frame = +2
Query: 20 TLKEANWSSVSSIIHKGGTIIGSARCMEFIKKERRFKAAYNLMSRGITNLAVIGGDGSLT 199
TL E + S+ + GG IIG C + K + M G+ LA G+L
Sbjct: 392 TLNEGGFQSIPYPVFPGGAIIG---CSAGFLNVPKIKGTHTAMKSGM--LAAEAAFGALH 446
Query: 200 GANLFRQEWSNLLD 241
W NL D
Sbjct: 447 EGLNMNTYWDNLRD 460
>At5g47810.1 68418.m05905 phosphofructokinase family protein similar
to phosphofructokinase [Amycolatopsis methanolica]
GI:17432243; contains Pfam profile PF00365:
Phosphofructokinase
Length = 444
Score = 26.6 bits (56), Expect = 3.2
Identities = 17/53 (32%), Positives = 31/53 (58%), Gaps = 1/53 (1%)
Frame = +2
Query: 65 KGGTIIGSARCMEFIKKERRFKAAYNLMSRGITNLAVIGGDGSLTGA-NLFRQ 220
KGGT++ ++R ++K A +L G + +IGGDG++ GA +F++
Sbjct: 140 KGGTVLATSRGGFHLQK---IVDAIHL--NGYNQVYIIGGDGTMRGAVEIFKE 187
>At2g32900.1 68415.m04033 centromere/kinetochore protein, putative
(ZW10) identical to centromere/kinetochore protein zw10
homolog SP:O48626 from [Arabidopsis thaliana]
Length = 742
Score = 26.6 bits (56), Expect = 3.2
Identities = 11/23 (47%), Positives = 13/23 (56%)
Frame = +2
Query: 185 DGSLTGANLFRQEWSNLLDELLE 253
DG L R+EWSN DE+ E
Sbjct: 167 DGEPVAYALLRKEWSNCFDEIQE 189
>At2g21370.2 68415.m02542 xylulose kinase, putative similar to
xylulose kinase (Xylulokinase) [Bacillus subtilis]
Swiss-Prot:P39211
Length = 385
Score = 26.6 bits (56), Expect = 3.2
Identities = 19/63 (30%), Positives = 32/63 (50%)
Frame = +2
Query: 65 KGGTIIGSARCMEFIKKERRFKAAYNLMSRGITNLAVIGGDGSLTGANLFRQEWSNLLDE 244
K T +GS ++ + +R A Y + S + + ++GG + TG + RQ +S DE
Sbjct: 200 KAVTSLGSTLAIKLLSTKRVDDARYGVYSHRLDDKWLVGGASN-TGGAILRQLFS---DE 255
Query: 245 LLE 253
LE
Sbjct: 256 QLE 258
>At2g21370.1 68415.m02543 xylulose kinase, putative similar to
xylulose kinase (Xylulokinase) [Bacillus subtilis]
Swiss-Prot:P39211
Length = 478
Score = 26.6 bits (56), Expect = 3.2
Identities = 19/63 (30%), Positives = 32/63 (50%)
Frame = +2
Query: 65 KGGTIIGSARCMEFIKKERRFKAAYNLMSRGITNLAVIGGDGSLTGANLFRQEWSNLLDE 244
K T +GS ++ + +R A Y + S + + ++GG + TG + RQ +S DE
Sbjct: 293 KAVTSLGSTLAIKLLSTKRVDDARYGVYSHRLDDKWLVGGASN-TGGAILRQLFS---DE 348
Query: 245 LLE 253
LE
Sbjct: 349 QLE 351
>At5g42960.1 68418.m05239 expressed protein
Length = 213
Score = 25.8 bits (54), Expect = 5.6
Identities = 11/29 (37%), Positives = 17/29 (58%)
Frame = -1
Query: 202 TSQRTITTNNRKIRYTAAH*VICGFETTF 116
++ + TNN KI+YT AH + FE +
Sbjct: 113 SANHMVGTNNCKIKYTYAHGGLATFEPCY 141
>At5g14770.1 68418.m01733 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 938
Score = 25.4 bits (53), Expect = 7.4
Identities = 16/65 (24%), Positives = 31/65 (47%), Gaps = 3/65 (4%)
Frame = +2
Query: 17 ITLKEANWSSVSSIIHKGGTIIGSARCMEFIKKERRFK---AAYNLMSRGITNLAVIGGD 187
+TL + N++S+ + KGG + E + +ER +YN++ G+ +G D
Sbjct: 500 VTLDQINYTSLIDVFFKGGDEEAALAWAEEM-QERGMPWDVVSYNVLISGMLKFGKVGAD 558
Query: 188 GSLTG 202
+ G
Sbjct: 559 WAYKG 563
>At5g04750.1 68418.m00488 F1F0-ATPase inhibitor protein, putative
similar to F1F0-ATPase inhibitor protein [Oryza sativa
(japonica cultivar-group)] gi|5106371|dbj|BAA81661
Length = 94
Score = 25.0 bits (52), Expect = 9.7
Identities = 11/28 (39%), Positives = 15/28 (53%)
Frame = -1
Query: 241 IKKVRPFLSEQIGTSQRTITTNNRKIRY 158
+K R F QIG S+ ++T IRY
Sbjct: 11 LKLARSFGERQIGASRSVVSTRGPAIRY 38
>At4g36090.1 68417.m05137 oxidoreductase, 2OG-Fe(II) oxygenase
family protein similar to At2g17970, At2g17960; contains
PF03171 2OG-Fe(II) oxygenase superfamily domain
Length = 385
Score = 25.0 bits (52), Expect = 9.7
Identities = 6/18 (33%), Positives = 16/18 (88%)
Frame = +2
Query: 251 ENNKITKDQREKYKFLHI 304
E K+++++RE+Y+F+++
Sbjct: 180 EKRKLSREERERYRFMNV 197
>At2g03420.1 68415.m00300 expressed protein
Length = 170
Score = 25.0 bits (52), Expect = 9.7
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = +2
Query: 227 SNLLDELLENNKITKDQREK 286
S LL +LLEN+K+ K++ E+
Sbjct: 109 SPLLKKLLENSKLNKEKNER 128
>At1g07220.1 68414.m00768 expressed protein
Length = 507
Score = 25.0 bits (52), Expect = 9.7
Identities = 10/33 (30%), Positives = 17/33 (51%)
Frame = +2
Query: 194 LTGANLFRQEWSNLLDELLENNKITKDQREKYK 292
L GA + RQ+W+ E +K++ +YK
Sbjct: 292 LWGAQIMRQDWAEEAKGGFEQSKLSNQCNHRYK 324
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,135,320
Number of Sequences: 28952
Number of extensions: 103915
Number of successful extensions: 332
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 327
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 329
length of database: 12,070,560
effective HSP length: 70
effective length of database: 10,043,920
effective search space used: 301317600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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