BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30914 (710 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 455 e-129 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 268 4e-73 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 252 3e-68 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 178 7e-46 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 96 4e-21 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 89 8e-19 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 89 8e-19 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 74 2e-14 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 68 2e-12 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 38 0.001 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.28 SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 3.5 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 4.6 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 455 bits (1121), Expect = e-129 Identities = 211/236 (89%), Positives = 223/236 (94%) Frame = +1 Query: 1 LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 180 LTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQI+F Sbjct: 65 LTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQIIF 124 Query: 181 ETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRD 360 ETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIYEGYALPHAI+RLDLAGRD Sbjct: 125 ETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRLDLAGRD 184 Query: 361 LTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPD 540 LTDYLMKIL ERGY+F+TTAEREIVRDIKEKLCYVALDFEQE+ TAA S+SLEKSYELPD Sbjct: 185 LTDYLMKILMERGYTFSTTAEREIVRDIKEKLCYVALDFEQELQTAAQSSSLEKSYELPD 244 Query: 541 GQVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMS 708 GQVITIGNERFR PEALFQPS LG+E+ GIHE YNSIMKCDVDIRKDLY N VMS Sbjct: 245 GQVITIGNERFRAPEALFQPSALGLENAGIHEATYNSIMKCDVDIRKDLYGNVVMS 300 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 268 bits (658), Expect = 4e-73 Identities = 120/236 (50%), Positives = 171/236 (72%) Frame = +1 Query: 1 LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 180 L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + MF Sbjct: 65 LQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETMF 124 Query: 181 ETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRD 360 E + +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE L H + RLD+AGRD Sbjct: 125 ERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVGRLDVAGRD 184 Query: 361 LTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPD 540 T YL+ +L +GY+F TA+ E VR++KEKLCYV+ D E + + +T L ++Y LPD Sbjct: 185 ATRYLISLLLRKGYAFNRTADFETVREMKEKLCYVSYDLELDHKLSEETTVLMRNYTLPD 244 Query: 541 GQVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMS 708 G+VI +G+ER+ CPE LFQP +G E G+ E ++++I DVDIRK LY V+S Sbjct: 245 GRVIKVGSERYECPECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLYRAIVLS 300 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 252 bits (618), Expect = 3e-68 Identities = 115/237 (48%), Positives = 164/237 (69%), Gaps = 1/237 (0%) Frame = +1 Query: 1 LTLKYPIEHGIITNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTQIM 177 L ++ PIE GII NW DME+IW + + ++ L PEEHP+LLTE PL N+EK+ + Sbjct: 69 LKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYF 128 Query: 178 FETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGR 357 +ET N PA+ ++Q VL+LYAS RTTGIVL+ GDG++H+VPIY+G+++P AI + ++ GR Sbjct: 129 YETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAIQQEEIGGR 188 Query: 358 DLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELP 537 D+TDYL L + G+ ++AE+EIVR+IKEK CYVA DF E+ + +Y+LP Sbjct: 189 DVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYVASDFRSEIESWTEHKPQIHTYQLP 248 Query: 538 DGQVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMS 708 D Q IT+G E F PE LF P +G E+ G+H ++ SI+ D+D+R LY+N V+S Sbjct: 249 DNQTITLGTECFSAPEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSNIVLS 305 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 178 bits (433), Expect = 7e-46 Identities = 103/250 (41%), Positives = 139/250 (55%), Gaps = 15/250 (6%) Frame = +1 Query: 4 TLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFE 183 +L YPI HG I NWD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE Sbjct: 89 SLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFE 148 Query: 184 TFNSPAMYVAIQAVLSLYASGRT--------TGIVLDSGDGVSHTVPIYEGYALPHAILR 339 +FN +Y+A+QAVL+L AS + TG V+DSGDGV+H +P+ EGY + +I Sbjct: 149 SFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIPVAEGYVIGSSIKT 208 Query: 340 LDLAGRDLTDYLMKILTERGY--SFTTTAEREIVRDIKEKLCYVALDFEQEMA--TAAAS 507 + LAGRD+T ++ +L +R S TAER IKE+ CYV D +E + Sbjct: 209 MPLAGRDVTYFVQSLLRDRNEPDSSLKTAER-----IKEECCYVCPDIVKEFSRFDREPD 263 Query: 508 TSLEKSYELPDGQ--VITIGNERFRCPEALFQPSFLGME-SCGIHETVYNSIMKCDVDIR 678 L+ + E G I +G ERF PE F P + + E V N + +D+R Sbjct: 264 RYLKYASESITGHSTTIDVGFERFLAPEIFFNPEIASSDFLTPLPELVDNVVQSSPIDVR 323 Query: 679 KDLYANTVMS 708 K LY N V+S Sbjct: 324 KGLYKNIVLS 333 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 96.3 bits (229), Expect = 4e-21 Identities = 51/171 (29%), Positives = 89/171 (52%), Gaps = 2/171 (1%) Frame = +1 Query: 16 PIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNS 195 P E +I+NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE +N+ Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144 Query: 196 PAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTD 369 P++ I + S Y + + ++GIVL+ G+ SH +P+ G + R+ G + Sbjct: 145 PSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVIPVLNGERILSEAKRISWGGSQSSS 204 Query: 370 YLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEK 522 YL+K+ + SF + C+V+ D+ ++A A LE+ Sbjct: 205 YLLKLFQIKYPSFPIKMLPSQAELLMHDHCHVSSDYTHDIAHALDRDILER 255 Score = 34.3 bits (75), Expect = 0.017 Identities = 13/48 (27%), Positives = 30/48 (62%) Frame = +1 Query: 565 ERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMS 708 E+ R PE +F PS +G++ GI E + + + + ++ ++ L +N +++ Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLIT 641 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 88.6 bits (210), Expect = 8e-19 Identities = 72/263 (27%), Positives = 115/263 (43%), Gaps = 36/263 (13%) Frame = +1 Query: 22 EHGIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEAPLNPKANREKMTQIMFETFNS 195 E G + +W +W N + P ++ +LLT+ + Q++FE F Sbjct: 64 EKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQPVFTMPSIEHNTIQLVFEEFQF 123 Query: 196 PAMYVAIQAVLSLYASGRTTG--------------IVLDSGDGVSHTVPIYEGYALPHAI 333 A A L + G T +V+DSG +H +P+ + A+ Sbjct: 124 DAYLPCTPAELIPWDHGSFTMNQEDAYTGQHGECVLVIDSGYSFTHIIPVIDFSVQEQAV 183 Query: 334 LRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATA--AAS 507 R+D+ G+ LT+YL ++++ R Y+ E IV +IKE +CYV+ +F+++M Sbjct: 184 KRVDVGGKLLTNYLKEVISYRKYNM--MEETYIVNEIKESVCYVSQNFKEDMEICHEKPR 241 Query: 508 TSLEKSYELPD------------------GQVITIGNERFRCPEALFQPSFLGMESCGIH 633 + LE Y LPD QV+ + NERF PE LF PS + + GI Sbjct: 242 SKLEICYALPDYSTGKHGYIVRDINQKIEQQVLNLSNERFMIPELLFSPSDIEIREAGIP 301 Query: 634 ETVYNSIMKCDVDIRKDLYANTV 702 E V S+ +I+ L N V Sbjct: 302 EAVMQSVTHFPENIQAMLLENVV 324 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 88.6 bits (210), Expect = 8e-19 Identities = 43/134 (32%), Positives = 69/134 (51%) Frame = +1 Query: 1 LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 180 + +K I +G + NWD +W + +L+ P EHP+L+TE NP NR K + MF Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122 Query: 181 ETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRD 360 E+ PA Y+A Q + +ASG+ T ++D G S IY+G+ L +G Sbjct: 123 ESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVLQKGYQVQHFSGNA 182 Query: 361 LTDYLMKILTERGY 402 + D L + L ++ + Sbjct: 183 INDILAQTLRDKNF 196 Score = 28.3 bits (60), Expect = 1.1 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = +1 Query: 625 GIHETVYNSIMKCDVDIRKDLYANTVM 705 GI + N I +CDVDIR L N ++ Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIV 352 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 74.1 bits (174), Expect = 2e-14 Identities = 34/128 (26%), Positives = 66/128 (51%) Frame = +1 Query: 25 HGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAM 204 +GII +W+ W +L+V P E+ +++TE NP++ R+++ + FE + PA Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133 Query: 205 YVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKI 384 Y+ QAV +A+ ++T +++D G + P+ +G + I + LAG L + ++ Sbjct: 134 YLTKQAVCVAFANSKSTALIVDIGSDNASVTPVVDGLIIRKGIFKQSLAGDFLNANIEQL 193 Query: 385 LTERGYSF 408 F Sbjct: 194 FNTMNIEF 201 Score = 60.9 bits (141), Expect = 2e-10 Identities = 38/111 (34%), Positives = 53/111 (47%), Gaps = 9/111 (8%) Frame = +1 Query: 403 SFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCP 582 S+ +R ++ + KE + V + A AS+ K +E PDG G ERFR Sbjct: 243 SYEIFQKRRVIEEWKESVLDV---LDTPFDEAKASSRNPKPFEFPDGVTHKFGQERFRIS 299 Query: 583 EALFQPSFLGMESC---------GIHETVYNSIMKCDVDIRKDLYANTVMS 708 E LF PSF S G+HE VY SI+ CD ++R L N V++ Sbjct: 300 EILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSELRSPLLNNIVVT 350 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 67.7 bits (158), Expect = 2e-12 Identities = 43/149 (28%), Positives = 70/149 (46%), Gaps = 4/149 (2%) Frame = +1 Query: 16 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 183 PI+ G + +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 184 TFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDL 363 P +A + ++ LYA G G+V+D G + PI +G + A +L L GR + Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLGGRYM 231 Query: 364 TDYLMKILTERGYSFTTTAEREIVRDIKE 450 T +L +L E + ++ + DI E Sbjct: 232 TQHLQNLLRESLPTLKSSGQYVSKEDITE 260 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 37.9 bits (84), Expect = 0.001 Identities = 28/146 (19%), Positives = 67/146 (45%), Gaps = 12/146 (8%) Frame = +1 Query: 7 LKYPIEHGIITNWD---------DMEKIWHHTFYNELRVAPEE---HPVLLTEAPLNPKA 150 L YPI+ G+ D D+ +I+ ++ + L++ + + V+ L + Sbjct: 198 LFYPIKSGVFNESDYASSQQLLADIYEIFKYSITSLLQIPVSQLSQYSVIFIVPDLYDRV 257 Query: 151 NREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHA 330 EK+ I+F + + +++ + + +G + V+D G + + EG +P++ Sbjct: 258 YVEKILDILFFDLHFGKAAIVQESLCTSFGAGMSAACVVDMGAQKTSISCVEEGVVVPNS 317 Query: 331 ILRLDLAGRDLTDYLMKILTERGYSF 408 ++++ G D+T MK+L + + Sbjct: 318 RIKINYGGDDITLLFMKLLMRSHFPY 343 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 30.3 bits (65), Expect = 0.28 Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%) Frame = +1 Query: 76 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 219 +Y L E+HP+LLT+ A L P+ + ++ +I ++ N+P + VAIQ Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451 >SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 248 Score = 26.6 bits (56), Expect = 3.5 Identities = 9/16 (56%), Positives = 9/16 (56%) Frame = +2 Query: 302 STKVTLCPTPSSVWTW 349 STK CP PS W W Sbjct: 52 STKTATCPDPSWEWAW 67 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 4.6 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +1 Query: 118 LLTEAPLNPKANREKMTQIMFETFN 192 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,517,163 Number of Sequences: 5004 Number of extensions: 47719 Number of successful extensions: 193 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 168 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 183 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 331187010 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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