BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30911 (735 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 436 e-123 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 251 7e-68 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 241 5e-65 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 165 6e-42 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 118 9e-28 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 111 8e-26 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 99 3e-22 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 66 6e-12 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 65 1e-11 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 31 0.17 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.30 SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 3.7 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 4.8 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 436 bits (1075), Expect = e-123 Identities = 199/222 (89%), Positives = 213/222 (95%) Frame = +1 Query: 70 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 249 ++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61 Query: 250 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 429 RGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQ Sbjct: 62 RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121 Query: 430 IMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLA 609 I+FETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIYEGYALPHAI+RLDLA Sbjct: 122 IIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRLDLA 181 Query: 610 GRDXTDYLMKILTERGYSFTTTAEXEIVRDIXXKLCYVALDF 735 GRD TDYLMKIL ERGY+F+TTAE EIVRDI KLCYVALDF Sbjct: 182 GRDLTDYLMKILMERGYTFSTTAEREIVRDIKEKLCYVALDF 223 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 251 bits (615), Expect = 7e-68 Identities = 116/221 (52%), Positives = 158/221 (71%), Gaps = 1/221 (0%) Frame = +1 Query: 76 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 255 D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67 Query: 256 ILTLKYPIEHGIITNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTQI 432 +L ++ PIE GII NW DME+IW + + ++ L PEEHP+LLTE PL N+EK+ + Sbjct: 68 LLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEY 127 Query: 433 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAG 612 +ET N PA+ ++Q VL+LYAS RTTGIVL+ GDG++H+VPIY+G+++P AI + ++ G Sbjct: 128 FYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAIQQEEIGG 187 Query: 613 RDXTDYLMKILTERGYSFTTTAEXEIVRDIXXKLCYVALDF 735 RD TDYL L + G+ ++AE EIVR+I K CYVA DF Sbjct: 188 RDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYVASDF 228 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 241 bits (591), Expect = 5e-65 Identities = 112/219 (51%), Positives = 157/219 (71%), Gaps = 2/219 (0%) Frame = +1 Query: 82 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRG 255 A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++ R Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63 Query: 256 ILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIM 435 +L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + M Sbjct: 64 LLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETM 123 Query: 436 FETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGR 615 FE + +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE L H + RLD+AGR Sbjct: 124 FERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVGRLDVAGR 183 Query: 616 DXTDYLMKILTERGYSFTTTAEXEIVRDIXXKLCYVALD 732 D T YL+ +L +GY+F TA+ E VR++ KLCYV+ D Sbjct: 184 DATRYLISLLLRKGYAFNRTADFETVREMKEKLCYVSYD 222 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 165 bits (401), Expect = 6e-42 Identities = 95/247 (38%), Positives = 137/247 (55%), Gaps = 32/247 (12%) Frame = +1 Query: 88 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 216 +++DNG+G K G+AG+DAP VFP+++ R G G M K Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66 Query: 217 ------DSYVGDEAQSKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 375 D ++G++A K +L YPI HG I NWD ME+ W + + LR PE+H Sbjct: 67 RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126 Query: 376 LLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRT--------TGIVLDS 531 LLTE PLNP NRE +IMFE+FN +Y+A+QAVL+L AS + TG V+DS Sbjct: 127 LLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDS 186 Query: 532 GDGVSHTVPIYEGYALPHAILRLDLAGRDXTDYLMKILTERGYSFTTTAEXEIVRDIXXK 711 GDGV+H +P+ EGY + +I + LAGRD T ++ +L +R ++ E +++ + Sbjct: 187 GDGVTHIIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDRNEPDSSLKTAERIKE---E 243 Query: 712 LCYVALD 732 CYV D Sbjct: 244 CCYVCPD 250 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 118 bits (284), Expect = 9e-28 Identities = 70/242 (28%), Positives = 128/242 (52%), Gaps = 3/242 (1%) Frame = +1 Query: 19 ESIPASPTQTHTKFKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVM 198 E + + PT + F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R + Sbjct: 8 EPVFSGPTPS---FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDRK-- 61 Query: 199 VGMGQKDSYVGDEAQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 375 + + + VG++ + G ++ + P E +I+NWD ME++ +TF +L + EHP+ Sbjct: 62 --LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPI 118 Query: 376 LLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSH 549 +TE NP R MT+++FE +N+P++ I + S Y + + ++GIVL+ G+ SH Sbjct: 119 CMTEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASH 178 Query: 550 TVPIYEGYALPHAILRLDLAGRDXTDYLMKILTERGYSFTTTAEXEIVRDIXXKLCYVAL 729 +P+ G + R+ G + YL+K+ + SF + C+V+ Sbjct: 179 VIPVLNGERILSEAKRISWGGSQSSSYLLKLFQIKYPSFPIKMLPSQAELLMHDHCHVSS 238 Query: 730 DF 735 D+ Sbjct: 239 DY 240 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 111 bits (268), Expect = 8e-26 Identities = 63/196 (32%), Positives = 100/196 (51%) Frame = +1 Query: 73 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 252 D+V+A+V+D GS + GF+G+D P+ V PS G + G + Y+ +S Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNP 61 Query: 253 GILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQI 432 G + +K I +G + NWD +W + +L+ P EHP+L+TE NP NR K + Sbjct: 62 G-MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLET 120 Query: 433 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAG 612 MFE+ PA Y+A Q + +ASG+ T ++D G S IY+G+ L +G Sbjct: 121 MFESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVLQKGYQVQHFSG 180 Query: 613 RDXTDYLMKILTERGY 660 D L + L ++ + Sbjct: 181 NAINDILAQTLRDKNF 196 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 99 bits (238), Expect = 3e-22 Identities = 52/182 (28%), Positives = 98/182 (53%), Gaps = 2/182 (1%) Frame = +1 Query: 73 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 252 +++ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61 Query: 253 GI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 426 I + +K +GII +W+ W +L+V P E+ +++TE NP++ R+++ Sbjct: 62 PIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIM 121 Query: 427 QIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDL 606 + FE + PA Y+ QAV +A+ ++T +++D G + P+ +G + I + L Sbjct: 122 EAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIGSDNASVTPVVDGLIIRKGIFKQSL 181 Query: 607 AG 612 AG Sbjct: 182 AG 183 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 65.7 bits (153), Expect = 6e-12 Identities = 40/130 (30%), Positives = 62/130 (47%), Gaps = 4/130 (3%) Frame = +1 Query: 274 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 441 PI+ G + +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 442 TFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDX 621 P +A + ++ LYA G G+V+D G + PI +G + A +L L GR Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLGGRYM 231 Query: 622 TDYLMKILTE 651 T +L +L E Sbjct: 232 TQHLQNLLRE 241 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 64.9 bits (151), Expect = 1e-11 Identities = 58/234 (24%), Positives = 103/234 (44%), Gaps = 18/234 (7%) Frame = +1 Query: 88 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 267 +V+DNG+ KAGFAG P+ + R + G + ++G+E + TL Sbjct: 8 IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSKD-------GNR-LFLGNELANCNDFTTL 57 Query: 268 KYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEAPLNPKANREKMTQIM 435 ++ H G + +W +W N + P ++ +LLT+ + Q++ Sbjct: 58 QFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQPVFTMPSIEHNTIQLV 117 Query: 436 FETFNSPAMYVAIQAVLSLYASGRTT--------------GIVLDSGDGVSHTVPIYEGY 573 FE F A A L + G T +V+DSG +H +P+ + Sbjct: 118 FEEFQFDAYLPCTPAELIPWDHGSFTMNQEDAYTGQHGECVLVIDSGYSFTHIIPVIDFS 177 Query: 574 ALPHAILRLDLAGRDXTDYLMKILTERGYSFTTTAEXEIVRDIXXKLCYVALDF 735 A+ R+D+ G+ T+YL ++++ R Y+ E IV +I +CYV+ +F Sbjct: 178 VQEQAVKRVDVGGKLLTNYLKEVISYRKYNM--MEETYIVNEIKESVCYVSQNF 229 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 31.1 bits (67), Expect = 0.17 Identities = 16/48 (33%), Positives = 26/48 (54%) Frame = +1 Query: 133 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 276 G+ PRA F ++ P H G+++ M KD G+E S +G + + P Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 30.3 bits (65), Expect = 0.30 Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%) Frame = +1 Query: 334 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 477 +Y L E+HP+LLT+ A L P+ + ++ +I ++ N+P + VAIQ Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451 >SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 248 Score = 26.6 bits (56), Expect = 3.7 Identities = 9/16 (56%), Positives = 9/16 (56%) Frame = +2 Query: 560 STKVTLCPTPSSVWTW 607 STK CP PS W W Sbjct: 52 STKTATCPDPSWEWAW 67 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 4.8 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +1 Query: 376 LLTEAPLNPKANREKMTQIMFETFN 450 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,744,365 Number of Sequences: 5004 Number of extensions: 55307 Number of successful extensions: 204 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 181 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 195 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 347244562 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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