Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30907
(751 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein... 69 9e-13
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 68 2e-12
SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ... 31 0.13
SPAC4F10.13c |mpd2||GYF domain|Schizosaccharomyces pombe|chr 1||... 27 2.2
SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma... 26 6.6
SPAC13G7.03 |||up-frameshift suppressor3 family|Schizosaccharomy... 26 6.6
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 26 6.6
>SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein
Stg1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 174
Score = 68.5 bits (160), Expect = 9e-13
Identities = 50/162 (30%), Positives = 80/162 (49%), Gaps = 2/162 (1%)
Frame = +2
Query: 101 EKEAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTGGQFKMMENI 280
EKEA+EWIE L K D L+ G +LC++ + ++ + + F MENI
Sbjct: 6 EKEAREWIEETLHTKLNAQLDLLDQLQSGVILCRICKEALGANI-RYKESNMPFVQMENI 64
Query: 281 TNF-QSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFALGRETYRHAEWSGPCLGPKPA 457
+ F A + VP D+FQT DL+E+++ QV+ ++ + R + LGPK A
Sbjct: 65 SAFINYAQQVVHVPSQDMFQTSDLFERRNDEQVLRSIHSFSRYAAKMFPGKVRGLGPKLA 124
Query: 458 DECKRDFS-DEVLKAGQTVIGLQAGSNKGATQSGQNLGAGRK 580
++ R FS + + + V LQ GS TQ + + R+
Sbjct: 125 EKKPRVFSAQQQREFREGVNSLQYGSFDMPTQGTEKIAFSRR 166
>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1489
Score = 67.7 bits (158), Expect = 2e-12
Identities = 39/124 (31%), Positives = 64/124 (51%), Gaps = 1/124 (0%)
Frame = +2
Query: 29 TQSHNMSLERQVRAKIASKRNPEKEKEAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLI 208
T+ N L + R + + + EA++WIE LG P FE L++G VL L+
Sbjct: 20 TKGSNTRLAAKQRETLQAYDYLCRVDEAKKWIEECLGTDLGPTSTFEQSLRNGVVLALLV 79
Query: 209 NKLKPGSVPKINTTGG-QFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKDIAQVVST 385
K +P + KI + QF+ +NI F I G+P+I F+ D++E K++ +V+
Sbjct: 80 QKFQPDKLIKIFYSNELQFRHSDNINKFLDFIHGIGLPEIFHFELTDIYEGKNLPKVIYC 139
Query: 386 LFAL 397
+ AL
Sbjct: 140 IHAL 143
>SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr
2|||Manual
Length = 962
Score = 31.5 bits (68), Expect = 0.13
Identities = 28/101 (27%), Positives = 49/101 (48%), Gaps = 4/101 (3%)
Frame = +2
Query: 107 EAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTGGQFKMMENITN 286
EA++W+E ++ + F D L +G VLCQL K P + ++++ E T
Sbjct: 69 EAKKWLEEETNNEYQNLDDFVDALVNGKVLCQLAFKYYP---KLASNWKPRYQISERNTV 125
Query: 287 FQSA----IKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFAL 397
+ +A + G+ F+T DL + +I +V+ L AL
Sbjct: 126 YLNAFFHFLDFIGMFTPFRFETKDLVRRFNIPKVIYCLHAL 166
>SPAC4F10.13c |mpd2||GYF domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 992
Score = 27.5 bits (58), Expect = 2.2
Identities = 18/48 (37%), Positives = 22/48 (45%), Gaps = 4/48 (8%)
Frame = -2
Query: 225 PGFSLLMSWQR----TVPSLRTSSNSSPGGNLAPRTPSIHSWASFSFS 94
PG L QR T P L TSS++ PGG + + HS S S
Sbjct: 210 PGVGTLQQPQRAGSDTFPDLNTSSSNQPGGEPNVASANTHSLEILSSS 257
>SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 614
Score = 25.8 bits (54), Expect = 6.6
Identities = 31/108 (28%), Positives = 47/108 (43%), Gaps = 16/108 (14%)
Frame = +2
Query: 89 NPEKEKEAQEWIEGVL------GAKFPPG-ELFE--DVLKDGTVLCQLINKLKPGSV--- 232
N E+ +E + I VL G++ P E FE D KDG +L +LIN P ++
Sbjct: 111 NEEERREFIKHINSVLAGDPDVGSRVPINTETFEFFDQCKDGLILSKLINDSVPDTIDER 170
Query: 233 ----PKINTTGGQFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKD 364
+ N FK +EN ++ KA G I D+ E ++
Sbjct: 171 VLNKQRNNKPLDNFKCIENNNVVINSAKAMGGISITNIGAGDILEGRE 218
>SPAC13G7.03 |||up-frameshift suppressor3 family|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 278
Score = 25.8 bits (54), Expect = 6.6
Identities = 19/80 (23%), Positives = 34/80 (42%)
Frame = +2
Query: 20 KALTQSHNMSLERQVRAKIASKRNPEKEKEAQEWIEGVLGAKFPPGELFEDVLKDGTVLC 199
K ++ S++ + + ++A K K + E +K P E V+K+ V
Sbjct: 166 KGKSKPSKKSVKAKKKLRLAEKPASNNSKAGKSSQESKKSSK-APAESAAAVIKEDKVSD 224
Query: 200 QLINKLKPGSVPKINTTGGQ 259
+ +K KP P N+T Q
Sbjct: 225 RKKSKKKPKKTPVSNSTASQ 244
>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
Pss1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 25.8 bits (54), Expect = 6.6
Identities = 16/41 (39%), Positives = 24/41 (58%)
Frame = +2
Query: 53 ERQVRAKIASKRNPEKEKEAQEWIEGVLGAKFPPGELFEDV 175
ER+ AK A+K EK+K + ++ EG G + PP E+V
Sbjct: 665 EREA-AKAATKSEAEKQKPSGKFEEGT-GGRAPPPPPAEEV 703
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,168,052
Number of Sequences: 5004
Number of extensions: 69389
Number of successful extensions: 176
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 171
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 176
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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