BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30873 (724 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 365 e-102 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 183 2e-47 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 173 2e-44 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 124 2e-29 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 97 2e-21 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 94 2e-20 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 85 7e-18 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 71 2e-13 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 43 4e-05 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 1.6 SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 27 2.7 SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosacc... 25 8.3 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 365 bits (898), Expect = e-102 Identities = 170/193 (88%), Positives = 182/193 (94%) Frame = +3 Query: 3 RDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYEL 182 RDLTDYLMKIL ERGY+F+TTAEREIVRDIKEKLCYVALDFEQE+ TAA S+SLEKSYEL Sbjct: 183 RDLTDYLMKILMERGYTFSTTAEREIVRDIKEKLCYVALDFEQELQTAAQSSSLEKSYEL 242 Query: 183 PDGQVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGG 362 PDGQVITIGNERFR PEALFQPS LG+E+ GIHE YNSIMKCDVDIRKDLY N VMSGG Sbjct: 243 PDGQVITIGNERFRAPEALFQPSALGLENAGIHEATYNSIMKCDVDIRKDLYGNVVMSGG 302 Query: 363 TTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEY 542 TTMYPGIADRMQKEI ALAPS++K+KI+APPERKYSVWIGGSILASLSTFQQMWISK+EY Sbjct: 303 TTMYPGIADRMQKEIQALAPSSMKVKIVAPPERKYSVWIGGSILASLSTFQQMWISKQEY 362 Query: 543 DESGPGIVHRKCF 581 DESGPGIV+RKCF Sbjct: 363 DESGPGIVYRKCF 375 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 183 bits (446), Expect = 2e-47 Identities = 85/193 (44%), Positives = 127/193 (65%) Frame = +3 Query: 3 RDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYEL 182 RD+TDYL L + G+ ++AE+EIVR+IKEK CYVA DF E+ + +Y+L Sbjct: 188 RDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYVASDFRSEIESWTEHKPQIHTYQL 247 Query: 183 PDGQVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGG 362 PD Q IT+G E F PE LF P +G E+ G+H ++ SI+ D+D+R LY+N V+SGG Sbjct: 248 PDNQTITLGTECFSAPEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSNIVLSGG 307 Query: 363 TTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEY 542 +T+ G +R E+ A++ ++KI A PER ++ W+GGSILASLSTF+++ I+ EEY Sbjct: 308 STLLRGFGERFISELRAISGKKNQVKIYASPERMHNAWLGGSILASLSTFRRLLITSEEY 367 Query: 543 DESGPGIVHRKCF 581 ++ ++ R+ F Sbjct: 368 -KNDQNVIFRRRF 379 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 173 bits (422), Expect = 2e-44 Identities = 86/196 (43%), Positives = 129/196 (65%), Gaps = 12/196 (6%) Frame = +3 Query: 3 RDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYEL 182 RD T YL+ +L +GY+F TA+ E VR++KEKLCYV+ D E + + +T L ++Y L Sbjct: 183 RDATRYLISLLLRKGYAFNRTADFETVREMKEKLCYVSYDLELDHKLSEETTVLMRNYTL 242 Query: 183 PDGQVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGG 362 PDG+VI +G+ER+ CPE LFQP +G E G+ E ++++I DVDIRK LY V+SGG Sbjct: 243 PDGRVIKVGSERYECPECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLYRAIVLSGG 302 Query: 363 TTMYPGIADRMQKEITAL--------APSTI---KIKIIAPPERKYSVWIGGSILAS-LS 506 ++MY G+ R++KEI L P+ + K+KI P R+++V+IGG++LA ++ Sbjct: 303 SSMYAGLPSRLEKEIKQLWFERVLHGDPARLPNFKVKIEDAPRRRHAVFIGGAVLADIMA 362 Query: 507 TFQQMWISKEEYDESG 554 MW+SK E++E G Sbjct: 363 QNDHMWVSKAEWEEYG 378 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 124 bits (298), Expect = 2e-29 Identities = 70/179 (39%), Positives = 97/179 (54%), Gaps = 11/179 (6%) Frame = +3 Query: 51 SFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCP 230 S+ +R ++ + KE + V + A AS+ K +E PDG G ERFR Sbjct: 243 SYEIFQKRRVIEEWKESVLDV---LDTPFDEAKASSRNPKPFEFPDGVTHKFGQERFRIS 299 Query: 231 EALFQPSFLGMESC---------GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGI 383 E LF PSF S G+HE VY SI+ CD ++R L N V++GGT++ PG+ Sbjct: 300 EILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSELRSPLLNNIVVTGGTSLIPGL 359 Query: 384 ADRMQKEITALAP-STIKIKII-APPERKYSVWIGGSILASLSTFQQMWISKEEYDESG 554 ++R+Q E+ LA S I + +VW GGSILASL FQ +W+SK+EYDE G Sbjct: 360 SERLQAEVQRLATGSRINVHTAETASATSNAVWFGGSILASLDNFQHLWVSKQEYDEVG 418 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 97.1 bits (231), Expect = 2e-21 Identities = 66/209 (31%), Positives = 101/209 (48%), Gaps = 20/209 (9%) Frame = +3 Query: 9 LTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAAS--TSLEKSYEL 182 LT+YL ++++ R Y+ E IV +IKE +CYV+ +F+++M + LE Y L Sbjct: 193 LTNYLKEVISYRKYNMME--ETYIVNEIKESVCYVSQNFKEDMEICHEKPRSKLEICYAL 250 Query: 183 PD------------------GQVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMK 308 PD QV+ + NERF PE LF PS + + GI E V S+ Sbjct: 251 PDYSTGKHGYIVRDINQKIEQQVLNLSNERFMIPELLFSPSDIEIREAGIPEAVMQSVTH 310 Query: 309 CDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGS 488 +I+ L N V GG +PG R+ E+ +LAP+ ++K+ P + W S Sbjct: 311 FPENIQAMLLENVVTIGGNCKFPGFHKRLSSELRSLAPANWEVKVFEPSDPICFPWKKAS 370 Query: 489 ILASLSTFQQMWISKEEYDESGPGIVHRK 575 + L + I++ EY E G I+ RK Sbjct: 371 HM-PLEHWNANKITRSEYSEHGANIMTRK 398 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 93.9 bits (223), Expect = 2e-20 Identities = 46/108 (42%), Positives = 70/108 (64%), Gaps = 6/108 (5%) Frame = +3 Query: 273 GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAP 452 GI + N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P + ++KI A Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHAS 384 Query: 453 P---ERKYSVWIGGSILASLSTFQQMWISKEEYDESGP---GIVHRKC 578 ER Y+ W+GGSIL+SL TF Q+WIS++EY+E G ++ ++C Sbjct: 385 GHVVERSYASWLGGSILSSLGTFHQLWISRQEYEEHGSDRLALIEKRC 432 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 85.4 bits (202), Expect = 7e-18 Identities = 63/211 (29%), Positives = 98/211 (46%), Gaps = 21/211 (9%) Frame = +3 Query: 3 RDLTDYLMKILTERGY--SFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTS--LEK 170 RD+T ++ +L +R S TAER IKE+ CYV D +E + L+ Sbjct: 214 RDVTYFVQSLLRDRNEPDSSLKTAER-----IKEECCYVCPDIVKEFSRFDREPDRYLKY 268 Query: 171 SYELPDGQVITI--GNERFRCPEALFQPSFLGMESCG-IHETVYNSIMKCDVDIRKDLYA 341 + E G TI G ERF PE F P + + E V N + +D+RK LY Sbjct: 269 ASESITGHSTTIDVGFERFLAPEIFFNPEIASSDFLTPLPELVDNVVQSSPIDVRKGLYK 328 Query: 342 NTVMSGGTTMYPGIADRMQKEITALAPSTI--------------KIKIIAPPERKYSVWI 479 N V+SGG+T++ +R+Q+++ + I + +I+ ++ +VW Sbjct: 329 NIVLSGGSTLFKNFGNRLQRDLKRIVDERIHRSEMLSGAKSGGVDVNVISHKRQRNAVWF 388 Query: 480 GGSILASLSTFQQMWISKEEYDESGPGIVHR 572 GGS+LA F +K +Y+E G I R Sbjct: 389 GGSLLAQTPEFGSYCHTKADYEEYGASIARR 419 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 70.9 bits (166), Expect = 2e-13 Identities = 32/115 (27%), Positives = 62/115 (53%) Frame = +3 Query: 213 ERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADR 392 E+ R PE +F PS +G++ GI E + + + + ++ ++ L +N +++GG PG+ R Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653 Query: 393 MQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGP 557 +++E+T++ P I + W G S + F+ +++EEY E GP Sbjct: 654 IKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTREEYLEKGP 708 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 43.2 bits (97), Expect = 4e-05 Identities = 20/86 (23%), Positives = 42/86 (48%), Gaps = 3/86 (3%) Frame = +3 Query: 327 KDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPP---ERKYSVWIGGSILA 497 K+LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586 Query: 498 SLSTFQQMWISKEEYDESGPGIVHRK 575 + ++WI ++ G ++ K Sbjct: 587 RIRIVSELWIKNSDWKMLGSRVLQYK 612 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 27.9 bits (59), Expect = 1.6 Identities = 13/37 (35%), Positives = 23/37 (62%), Gaps = 3/37 (8%) Frame = +3 Query: 474 WIGGSILASLSTFQQM---WISKEEYDESGPGIVHRK 575 ++GGSI+A S + + +++ EEY + GP +H K Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522 >SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 617 Score = 27.1 bits (57), Expect = 2.7 Identities = 14/37 (37%), Positives = 20/37 (54%) Frame = +3 Query: 399 KEITALAPSTIKIKIIAPPERKYSVWIGGSILASLST 509 KEI++ A S I + + P E+ W ILA L+T Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248 >SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 284 Score = 25.4 bits (53), Expect = 8.3 Identities = 15/43 (34%), Positives = 20/43 (46%) Frame = +2 Query: 278 PRDRVQLHHEVRRRHP*GPVRQHRHVRWYHHVPRYRRQDAEGD 406 PR+ V R+R P P + R V PR RRQ +G+ Sbjct: 41 PRELVAQSTTSRKRDPNQPTPRERTVNKKADQPRRRRQAPQGN 83 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,541,941 Number of Sequences: 5004 Number of extensions: 49413 Number of successful extensions: 151 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 136 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 143 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 339215786 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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