BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30871 (789 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 32 0.11 SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|ch... 32 0.11 SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schiz... 31 0.19 SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 31 0.19 SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 31 0.25 SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyc... 28 1.3 SPBC649.05 |cut12|stf1|spindle pole body protein Cut12 |Schizosa... 28 1.8 SPAC3H1.11 |hsr1||transcription factor Hsr1|Schizosaccharomyces ... 27 2.3 SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25 |Schizosac... 27 2.3 SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe... 27 2.3 SPBC365.07c |||TATA element modulatory factor homolog |Schizosac... 27 2.3 SPCC2H8.05c ||SPCC63.01c|sequence orphan|Schizosaccharomyces pom... 27 3.1 SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosacc... 27 4.0 SPAC688.11 |end4|sla2|Huntingtin-interacting protein homolog|Sch... 26 5.4 SPCC830.08c |||Golgi membrane protein |Schizosaccharomyces pombe... 26 7.1 SPAC27D7.14c |tpr1|SPAC637.02c|RNA polymerase II associated Paf1... 26 7.1 SPBC776.13 |cnd1||condensin subunit Cnd1|Schizosaccharomyces pom... 26 7.1 SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Sch... 26 7.1 SPAC29B12.02c |set2||histone lysine methyltransferase Set2 |Schi... 25 9.4 SPAC4H3.06 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 25 9.4 SPAC869.01 |||amidase |Schizosaccharomyces pombe|chr 1|||Manual 25 9.4 SPAC30D11.09 |cwf19||complexed with Cdc5 protein Cwf19 |Schizosa... 25 9.4 >SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1085 Score = 31.9 bits (69), Expect = 0.11 Identities = 36/181 (19%), Positives = 73/181 (40%), Gaps = 9/181 (4%) Frame = +3 Query: 6 RRAPGRPRKAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKE----EEFENTRKNHQR- 170 R + R+A+ E EE + + EL ++R++ +R ++K+ +E E R+ +R Sbjct: 561 REKVAQQRQAKLLEEIEEENKRKQERELKKIREKEKKRDKKKQLKLAKEEERQRREAERL 620 Query: 171 ----ALDSMQXXXXXXXXXXXXXXRMKKKLEADINELEIALDHANKANAEAQKNIKRYQA 338 A +++ R+K++ E ELE + E +K +K+ Q Sbjct: 621 AEQAAQKALEAKRQEEARKKREEQRLKREQEKKQQELERQKREEKQKQKEREKKLKKQQQ 680 Query: 339 QIKDLQTALXXXXXXXXXXXXXLGISERRANALQNELEESRTLLEQADRARRQAEQELSD 518 + + A + ER+ ++ EE R E ++ + E+ L + Sbjct: 681 EADREKMAREQRLREEEEKRI---LEERKRREKLDKEEEERRRRELLEKESEEKERRLRE 737 Query: 519 A 521 A Sbjct: 738 A 738 >SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1208 Score = 31.9 bits (69), Expect = 0.11 Identities = 31/151 (20%), Positives = 56/151 (37%), Gaps = 1/151 (0%) Frame = +3 Query: 27 RKAEAALEQEENKV-LRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQXXXXX 203 R +++E+N + L A + E + R+QE +E E R + L+ + Sbjct: 469 RTQNREIDEEKNHLRLLASKNSDKALAETNIRLQEVTKELETLRMKNSNDLNEIHDLREE 528 Query: 204 XXXXXXXXXRMKKKLEADINELEIALDHANKANAEAQKNIKRYQAQIKDLQTALXXXXXX 383 + K+ + INELE + +E I Y+ ++KD + Sbjct: 529 NEGLTLKIDSITKEKDRLINELEQRIKSYEVNVSELNGTIDEYRNKLKDKEETYNEVMNA 588 Query: 384 XXXXXXXLGISERRANALQNELEESRTLLEQ 476 L N LQ+ +E + LE+ Sbjct: 589 FQYKDNDLRRFHESINKLQDREKELTSNLEK 619 >SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 527 Score = 31.1 bits (67), Expect = 0.19 Identities = 23/92 (25%), Positives = 44/92 (47%), Gaps = 4/92 (4%) Frame = +3 Query: 264 ELEIALDHANKANAEAQKNIKRYQAQIKDLQTALXXXXXXXXXXXXXLGI-SERRANALQ 440 E ++ L NAE++K+ R + +++DL ++L + SE+R N L+ Sbjct: 87 EEKLQLTETKCRNAESEKS--RVENELEDLTSSLFEEANRMVANARKETVASEKRVNQLK 144 Query: 441 NELEESRTLLEQADRARRQAE---QELSDAHE 527 +L ++ TLL + + +SD+HE Sbjct: 145 KQLVDAETLLSSTQHQLTELKDVMHSMSDSHE 176 >SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1044 Score = 31.1 bits (67), Expect = 0.19 Identities = 27/93 (29%), Positives = 39/93 (41%), Gaps = 1/93 (1%) Frame = +3 Query: 240 KKLEADINELEIALDHANKANAEAQKN-IKRYQAQIKDLQTALXXXXXXXXXXXXXLGIS 416 KKLE D+N L L+ N A+ E+ KN I+ + K L L L Sbjct: 753 KKLENDLNLLTEKLNKKN-ADTESFKNTIREAELSKKALNDNLGNKENIISDLKNKLSEE 811 Query: 417 ERRANALQNELEESRTLLEQADRARRQAEQELS 515 R LQ++L + + +E + A ELS Sbjct: 812 STRLQELQSQLNQDKNQIETLNERISAAADELS 844 >SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces pombe|chr 1|||Manual Length = 1957 Score = 30.7 bits (66), Expect = 0.25 Identities = 27/161 (16%), Positives = 67/161 (41%), Gaps = 8/161 (4%) Frame = +3 Query: 54 EENKVLRAQLELSQVR-QEIDRRIQEKEEEFENTRKNHQRALDSMQXXXXXXXXXXXXXX 230 ++++ +QLE V +E +R I + + E+ + ++ S++ Sbjct: 360 KDSRTSNSQLEEEMVELKESNRTIHSQLTDAESKLSSFEQENKSLKGSIDEYQNNLSSKD 419 Query: 231 RMKKKLEADINELEIALDHANKANAE-------AQKNIKRYQAQIKDLQTALXXXXXXXX 389 +M K++ + + E +L HA AE K IK ++ +DL+ L Sbjct: 420 KMVKQVSSQLEEARSSLAHATGKLAEINSERDFQNKKIKDFEKIEQDLRACLNSSSNELK 479 Query: 390 XXXXXLGISERRANALQNELEESRTLLEQADRARRQAEQEL 512 + ++ N L+ +++E + + E + + ++++ Sbjct: 480 EKSALIDKKDQELNNLREQIKEQKKVSESTQSSLQSLQRDI 520 Score = 25.8 bits (54), Expect = 7.1 Identities = 20/112 (17%), Positives = 47/112 (41%), Gaps = 6/112 (5%) Frame = +3 Query: 36 EAALEQEENKVLRAQLELSQVRQEIDRR---IQEKEEEFENTRKNHQRALDSMQXXXXXX 206 E+ L E N + L+L + + + ++ + E+ + +++ +++ ++++ Sbjct: 653 ESTLSDENNDLRTKLLKLEESNKSLIKKQEDVDSLEKNIQTLKEDLRKSEEALRFSKLEA 712 Query: 207 XXXXXXXXRMKKK---LEADINELEIALDHANKANAEAQKNIKRYQAQIKDL 353 +K K LEA N+L +L A NA + + +K L Sbjct: 713 KNLREVIDNLKGKHETLEAQRNDLHSSLSDAKNTNAILSSELTKSSEDVKRL 764 >SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyces pombe|chr 1|||Manual Length = 1085 Score = 28.3 bits (60), Expect = 1.3 Identities = 12/30 (40%), Positives = 21/30 (70%), Gaps = 1/30 (3%) Frame = +3 Query: 102 QEIDRRIQE-KEEEFENTRKNHQRALDSMQ 188 Q +DR +Q+ KE +NT++ HQ+ LD ++ Sbjct: 912 QTLDRLLQKVKEHSEDNTKEKHQQLLDLLE 941 >SPBC649.05 |cut12|stf1|spindle pole body protein Cut12 |Schizosaccharomyces pombe|chr 2|||Manual Length = 548 Score = 27.9 bits (59), Expect = 1.8 Identities = 14/35 (40%), Positives = 23/35 (65%) Frame = +3 Query: 78 QLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDS 182 +LEL+ +R+ ++ I EK+EE N K RAL++ Sbjct: 275 ELELTSIRERLESLILEKQEEI-NFWKQRCRALET 308 >SPAC3H1.11 |hsr1||transcription factor Hsr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 582 Score = 27.5 bits (58), Expect = 2.3 Identities = 12/24 (50%), Positives = 15/24 (62%) Frame = +1 Query: 112 TDASKRRRKNSKTHARTTSVPSTP 183 TD S +RRK + R TSVP +P Sbjct: 395 TDGSMKRRKRRQPSNRKTSVPRSP 418 >SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25 |Schizosaccharomyces pombe|chr 2|||Manual Length = 376 Score = 27.5 bits (58), Expect = 2.3 Identities = 12/44 (27%), Positives = 24/44 (54%) Frame = +3 Query: 42 ALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRA 173 A++Q+E K L+ +E + + DR+ +E+ + R N R+ Sbjct: 141 AIKQQEQKQLQTLMEKRKYSLDSDRKSKERRHRDRHHRSNQDRS 184 >SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 830 Score = 27.5 bits (58), Expect = 2.3 Identities = 15/44 (34%), Positives = 28/44 (63%), Gaps = 1/44 (2%) Frame = +1 Query: 49 NKKRTRSSVLSSNCLRSDRRSTDASKRRRK-NSKTHARTTSVPS 177 ++KR S+ S++ +SDR S +S R+ S++ +R++SV S Sbjct: 79 SRKRVSFSLTSNDGAKSDRSSQKSSNSRKSIRSQSRSRSSSVGS 122 >SPBC365.07c |||TATA element modulatory factor homolog |Schizosaccharomyces pombe|chr 2|||Manual Length = 547 Score = 27.5 bits (58), Expect = 2.3 Identities = 17/54 (31%), Positives = 31/54 (57%) Frame = +3 Query: 27 RKAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQ 188 ++A L+ ++K + QLEL + + E I EKE+E + RK + ++SM+ Sbjct: 86 KQATPELQVSDSKEMEEQLELQKSQFEKRISILEKEKE-DLQRKMEELTVESME 138 >SPCC2H8.05c ||SPCC63.01c|sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 217 Score = 27.1 bits (57), Expect = 3.1 Identities = 24/97 (24%), Positives = 40/97 (41%), Gaps = 9/97 (9%) Frame = +3 Query: 21 RPRKAEAALEQEE---NKVLRAQLELSQVRQEID------RRIQEKEEEFENTRKNHQRA 173 R R E++ E E NK+ R +S +RQ ID RR++E++EE + ++ Sbjct: 89 RSRTLESSYEDETETANKLSRVSSLVSVIRQTIDRKKSLERRVREEQEEKTDNEDDNDVE 148 Query: 174 LDSMQXXXXXXXXXXXXXXRMKKKLEADINELEIALD 284 + + + LE DI E + D Sbjct: 149 ISTQESLENNGLAEKKDDTSSLATLEDDIEGQEFSFD 185 >SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 1106 Score = 26.6 bits (56), Expect = 4.0 Identities = 12/29 (41%), Positives = 17/29 (58%) Frame = +3 Query: 243 KLEADINELEIALDHANKANAEAQKNIKR 329 KL + + L LDH+N +AE + IKR Sbjct: 430 KLGPEYSSLSAILDHSNNIHAEILETIKR 458 >SPAC688.11 |end4|sla2|Huntingtin-interacting protein homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1092 Score = 26.2 bits (55), Expect = 5.4 Identities = 12/37 (32%), Positives = 19/37 (51%) Frame = +3 Query: 36 EAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFE 146 E AL ++ ++ +EL + +QEKEEE E Sbjct: 598 EDALHSKDRELANLGVELKSTENRYRQLLQEKEEELE 634 >SPCC830.08c |||Golgi membrane protein |Schizosaccharomyces pombe|chr 3|||Manual Length = 182 Score = 25.8 bits (54), Expect = 7.1 Identities = 13/32 (40%), Positives = 20/32 (62%) Frame = +1 Query: 331 TRHKSRICRPLSRRSSAPVTMPANSSASRSVA 426 T H RIC+ +SR+++AP P SS + + A Sbjct: 146 TPHVIRICKSVSRQNAAPA--PTASSFAHTTA 175 >SPAC27D7.14c |tpr1|SPAC637.02c|RNA polymerase II associated Paf1 complex subunit Tpr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1039 Score = 25.8 bits (54), Expect = 7.1 Identities = 17/61 (27%), Positives = 32/61 (52%), Gaps = 4/61 (6%) Frame = +3 Query: 3 ERRAPGRPRKAEAALEQEENKVLRAQLELSQVRQEIDRRIQEK----EEEFENTRKNHQR 170 E+ + A E+E+ + L + L + +QE +R++QE+ +EE RK+ Q+ Sbjct: 864 EKSVAAKLEDARIQREKEKARRLAEEEALLKEKQERERQLQEERQKMQEEVLEWRKSQQK 923 Query: 171 A 173 A Sbjct: 924 A 924 >SPBC776.13 |cnd1||condensin subunit Cnd1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1158 Score = 25.8 bits (54), Expect = 7.1 Identities = 14/37 (37%), Positives = 21/37 (56%) Frame = +1 Query: 61 TRSSVLSSNCLRSDRRSTDASKRRRKNSKTHARTTSV 171 T +S+ SSN L R ST+ K R+KN T++ + Sbjct: 121 TVNSLSSSNQLSVIRSSTNTVKGRKKNPTTNSNWNGI 157 >SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1194 Score = 25.8 bits (54), Expect = 7.1 Identities = 12/28 (42%), Positives = 18/28 (64%) Frame = +3 Query: 282 DHANKANAEAQKNIKRYQAQIKDLQTAL 365 D+ N + +A KN+K YQ + DLQ +L Sbjct: 661 DYRN-SRLDAIKNVKTYQIKFSDLQESL 687 >SPAC29B12.02c |set2||histone lysine methyltransferase Set2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 798 Score = 25.4 bits (53), Expect = 9.4 Identities = 18/55 (32%), Positives = 30/55 (54%), Gaps = 5/55 (9%) Frame = +3 Query: 39 AALEQEENK-VLRAQLE----LSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQ 188 AA+E K VL+AQ E ++Q ++E RR+ +E + +K H++ S Q Sbjct: 627 AAMESVNQKNVLKAQKEEEERIAQQKREEKRRLAYEESLKRHAKKLHEKKTKSSQ 681 >SPAC4H3.06 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 131 Score = 25.4 bits (53), Expect = 9.4 Identities = 10/32 (31%), Positives = 21/32 (65%) Frame = +3 Query: 45 LEQEENKVLRAQLELSQVRQEIDRRIQEKEEE 140 +E+ NK+LRAQ +Q+ +E++ + +E+ Sbjct: 1 MEETVNKILRAQETRAQLYKELEDALNANQEK 32 >SPAC869.01 |||amidase |Schizosaccharomyces pombe|chr 1|||Manual Length = 583 Score = 25.4 bits (53), Expect = 9.4 Identities = 12/38 (31%), Positives = 21/38 (55%) Frame = -1 Query: 138 LPPSLGCVCRSPV*PETVRAEHGGPCSLLVREPLRLFE 25 L P++G R V PE+ + GP + VR+ + +F+ Sbjct: 259 LKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQ 296 >SPAC30D11.09 |cwf19||complexed with Cdc5 protein Cwf19 |Schizosaccharomyces pombe|chr 1|||Manual Length = 639 Score = 25.4 bits (53), Expect = 9.4 Identities = 12/39 (30%), Positives = 24/39 (61%) Frame = +3 Query: 36 EAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENT 152 E+ L + + +LRAQL+ + ++R Q+ ++FEN+ Sbjct: 273 ESDLNRLKADLLRAQLKKDPNYESLEREYQKACDDFENS 311 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,118,153 Number of Sequences: 5004 Number of extensions: 31184 Number of successful extensions: 198 Number of sequences better than 10.0: 22 Number of HSP's better than 10.0 without gapping: 185 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 198 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 383374054 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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