Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30850
(726 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 241 3e-64
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 237 5e-63
At2g47550.1 68415.m05934 pectinesterase family protein contains ... 31 1.0
At2g43680.2 68415.m05430 calmodulin-binding family protein simil... 30 1.4
At2g43680.1 68415.m05429 calmodulin-binding family protein simil... 30 1.4
At4g34220.1 68417.m04862 leucine-rich repeat transmembrane prote... 29 3.1
At1g53120.1 68414.m06015 RNA-binding S4 domain-containing protein 29 4.1
At1g18670.1 68414.m02330 protein kinase family protein contains ... 29 4.1
At1g26400.1 68414.m03220 FAD-binding domain-containing protein s... 28 5.5
At4g12470.1 68417.m01972 protease inhibitor/seed storage/lipid t... 28 7.2
At4g19515.1 68417.m02871 disease resistance family protein simil... 27 9.6
At1g64110.2 68414.m07264 AAA-type ATPase family protein contains... 27 9.6
At1g64110.1 68414.m07263 AAA-type ATPase family protein contains... 27 9.6
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 241 bits (590), Expect = 3e-64
Identities = 117/243 (48%), Positives = 150/243 (61%), Gaps = 1/243 (0%)
Frame = +1
Query: 1 GQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXXXXXXXXXXXXXXXXXXXQARGHIIEKI 180
GQ AFGNMCRGGRMFAPTK WRRWH ARGH IE +
Sbjct: 93 GQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNMKRHAIVSAIAATAVPALVMARGHKIENV 152
Query: 181 PELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRKGP 360
PE+PLVV+D + + KT A+ L+++ A+ D K S +R GKGKMRNRR I RKGP
Sbjct: 153 PEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRNRRYISRKGP 212
Query: 361 LIIFNKDQG-LTRAFRNIPGVEXXXXXXXXXXXXAPGGHLGRFVIWTQSAFGRLDPLFGS 537
L+++ + + +AFRN+PGVE APGGHLGRFVIWT+SAF +L+ ++GS
Sbjct: 213 LVVYGTEGSKIVKAFRNLPGVELCHVERLNLLKLAPGGHLGRFVIWTKSAFEKLESIYGS 272
Query: 538 WKTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAML 717
++ PS++KK + LP+ KM N DL R++ SDEI+ V+ K RA K NPL N ML
Sbjct: 273 FEKPSEKKKGYVLPRAKMVNADLARIINSDEIQSVVNPIKKDAKRAVLKKNPLKNLNVML 332
Query: 718 KLN 726
KLN
Sbjct: 333 KLN 335
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 237 bits (580), Expect = 5e-63
Identities = 115/243 (47%), Positives = 148/243 (60%), Gaps = 1/243 (0%)
Frame = +1
Query: 1 GQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXXXXXXXXXXXXXXXXXXXQARGHIIEKI 180
GQ AFGNMCRGGRMFAPTK WRRWH ARGH IE +
Sbjct: 94 GQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNMKRHAIVSAIAATAVPALVMARGHKIENV 153
Query: 181 PELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRKGP 360
PE+PLVV+D + + KT A+ L+++ A+ D K S +R GKGKMRNRR I RKGP
Sbjct: 154 PEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRNRRYISRKGP 213
Query: 361 LIIFNKDQG-LTRAFRNIPGVEXXXXXXXXXXXXAPGGHLGRFVIWTQSAFGRLDPLFGS 537
L++F + + +AFRN+PGVE APGGHLGRFVIWT+SAF +L+ ++GS
Sbjct: 214 LVVFGTEGAKIVKAFRNLPGVELCHVERLNLLKLAPGGHLGRFVIWTKSAFEKLESIYGS 273
Query: 538 WKTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAML 717
++ PS++KK + LP+ KM N DL R++ SDE++ V+ RA K NPL N M
Sbjct: 274 FEKPSEKKKGYVLPRAKMVNADLARIINSDEVQSVVNPIKDGSKRAVLKKNPLKNLNVMF 333
Query: 718 KLN 726
KLN
Sbjct: 334 KLN 336
>At2g47550.1 68415.m05934 pectinesterase family protein contains
Pfam profile: PF01095 pectinesterase
Length = 560
Score = 30.7 bits (66), Expect = 1.0
Identities = 27/96 (28%), Positives = 45/96 (46%), Gaps = 2/96 (2%)
Frame = -1
Query: 447 VQLVNIQELHTGDVAESASQTLILVED--YEGSLTLDTTTVAHFTLTSTKTLRLVHLKDI 274
+ +++ + G VA A T+ +ED + SLT+D + T STKTL L +D+
Sbjct: 78 ISMIDAELDRKGKVA--AKSTVGALEDCKFLASLTMDYLLSSSQTADSTKTLSLSRAEDV 135
Query: 273 RPCLEAPQEDDSLFGLVDLLDFVGYNQGKLGNLFNN 166
L A ++ ++ L G G+LFN+
Sbjct: 136 HTFLSAAITNEQT--CLEGLKSTASENGLSGDLFND 169
>At2g43680.2 68415.m05430 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 669
Score = 30.3 bits (65), Expect = 1.4
Identities = 19/61 (31%), Positives = 26/61 (42%)
Frame = +3
Query: 30 WWTYVRPHEALAALAPSRQPPTAESGLGSSRCCYWRPSARSG*RTHY*KDSRASLGCSRQ 209
WW +V LA+ APS P + L SR C P ++S + H D+ R
Sbjct: 465 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLCP-SPLSQSSKQHHIRLDNHFDTSTPRS 523
Query: 210 S 212
S
Sbjct: 524 S 524
>At2g43680.1 68415.m05429 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 668
Score = 30.3 bits (65), Expect = 1.4
Identities = 19/61 (31%), Positives = 26/61 (42%)
Frame = +3
Query: 30 WWTYVRPHEALAALAPSRQPPTAESGLGSSRCCYWRPSARSG*RTHY*KDSRASLGCSRQ 209
WW +V LA+ APS P + L SR C P ++S + H D+ R
Sbjct: 464 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLCP-SPLSQSSKQHHIRLDNHFDTSTPRS 522
Query: 210 S 212
S
Sbjct: 523 S 523
>At4g34220.1 68417.m04862 leucine-rich repeat transmembrane protein
kinase, putative protein kinase TMKL1, Arabidopsis
thaliana, PID:E353150
Length = 757
Score = 29.1 bits (62), Expect = 3.1
Identities = 22/68 (32%), Positives = 31/68 (45%)
Frame = -1
Query: 489 DETSKMTSRSQLQEVQLVNIQELHTGDVAESASQTLILVEDYEGSLTLDTTTVAHFTLTS 310
DETS TS S ++ Q V G + +SQT ++ D E L LDT A +
Sbjct: 421 DETS--TSESDVENQQTVQAFTRTDGGQLKQSSQTQLVTVDGETRLDLDTLLKASAYILG 478
Query: 309 TKTLRLVH 286
T +V+
Sbjct: 479 TTGTGIVY 486
>At1g53120.1 68414.m06015 RNA-binding S4 domain-containing protein
Length = 320
Score = 28.7 bits (61), Expect = 4.1
Identities = 15/63 (23%), Positives = 31/63 (49%)
Frame = -1
Query: 363 EGSLTLDTTTVAHFTLTSTKTLRLVHLKDIRPCLEAPQEDDSLFGLVDLLDFVGYNQGKL 184
E SL +D A F ++ +K + L+ KD+R ++ ++ D++ G + K+
Sbjct: 242 EASLRIDAVASAGFKISRSKLVDLISSKDVRVNWATVTKNGTIVKTGDVVSVSGKGRLKI 301
Query: 183 GNL 175
G +
Sbjct: 302 GEI 304
>At1g18670.1 68414.m02330 protein kinase family protein contains
Protein kinases ATP-binding region signature,
PROSITE:PS00107 and Serine/Threonine protein kinases
active-site signature, PROSITE:PS00108
Length = 662
Score = 28.7 bits (61), Expect = 4.1
Identities = 18/54 (33%), Positives = 26/54 (48%)
Frame = +3
Query: 147 RSG*RTHY*KDSRASLGCSRQSPGDQQDQTGCHLPEAPQGMV*YP*GVQVSASS 308
R+G H DS ++L Q P + + H+ A QG V + +QVS SS
Sbjct: 491 RNGHSVHNSIDSDSTLFEKMQKPSNHEKDEASHVKNASQGDVPFSGPLQVSVSS 544
>At1g26400.1 68414.m03220 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 527
Score = 28.3 bits (60), Expect = 5.5
Identities = 13/22 (59%), Positives = 16/22 (72%)
Frame = -2
Query: 236 CLVLLISWTLSATTKGSSGIFS 171
CLVLL+S +A TK SGIF+
Sbjct: 9 CLVLLVSILRAAVTKPDSGIFT 30
>At4g12470.1 68417.m01972 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein similar to pEARLI
1 (Accession No. L43080): an Arabidopsis member of a
conserved gene family (PGF95-099), Plant Physiol. 109
(4), 1497 (1995); contains Pfam protease inhibitor/seed
storage/LTP family domain PF00234
Length = 161
Score = 27.9 bits (59), Expect = 7.2
Identities = 18/46 (39%), Positives = 22/46 (47%)
Frame = -1
Query: 189 KLGNLFNNVSSSLNERWDASSSNGCCQGRSPLSEVDATVPAPPGLR 52
KLG N +SS LN + SS CC L +VDA + LR
Sbjct: 84 KLGVCANVLSSLLNIQLGQPSSQQCCSLIQGLVDVDAAICLCTALR 129
>At4g19515.1 68417.m02871 disease resistance family protein similar
to downy mildew resistance protein RPP5 [Arabidopsis
thaliana] GI:6449046; contains Pfam profile PF01582: TIR
domain
Length = 524
Score = 27.5 bits (58), Expect = 9.6
Identities = 16/51 (31%), Positives = 25/51 (49%)
Frame = -1
Query: 450 EVQLVNIQELHTGDVAESASQTLILVEDYEGSLTLDTTTVAHFTLTSTKTL 298
E+ VN QE+H GD + ++ D GS + +T T ++ S K L
Sbjct: 101 EIFPVNKQEMHVGDSCTITKCGVYVINDAAGSSSGNTMTPQCSSMDSLKLL 151
>At1g64110.2 68414.m07264 AAA-type ATPase family protein contains
Pfam domain, PF00004: ATPase, AAA family
Length = 829
Score = 27.5 bits (58), Expect = 9.6
Identities = 18/77 (23%), Positives = 42/77 (54%), Gaps = 4/77 (5%)
Frame = -1
Query: 495 PDDETSKMTSRSQLQ-EVQLVNIQELHTGDVAESASQTLILVEDYEGSLTLDTTTVAHF- 322
P+DET ++ +SQL+ ++ ++ Q+ + + E S+ ++ +D E DT ++++
Sbjct: 337 PEDETHLVSWKSQLERDMNMIQTQD-NRNHIMEVLSENDLICDDLESISFEDTKVLSNYI 395
Query: 321 --TLTSTKTLRLVHLKD 277
+ S + L++ KD
Sbjct: 396 EEIVVSALSYHLMNNKD 412
>At1g64110.1 68414.m07263 AAA-type ATPase family protein contains
Pfam domain, PF00004: ATPase, AAA family
Length = 824
Score = 27.5 bits (58), Expect = 9.6
Identities = 18/77 (23%), Positives = 42/77 (54%), Gaps = 4/77 (5%)
Frame = -1
Query: 495 PDDETSKMTSRSQLQ-EVQLVNIQELHTGDVAESASQTLILVEDYEGSLTLDTTTVAHF- 322
P+DET ++ +SQL+ ++ ++ Q+ + + E S+ ++ +D E DT ++++
Sbjct: 332 PEDETHLVSWKSQLERDMNMIQTQD-NRNHIMEVLSENDLICDDLESISFEDTKVLSNYI 390
Query: 321 --TLTSTKTLRLVHLKD 277
+ S + L++ KD
Sbjct: 391 EEIVVSALSYHLMNNKD 407
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,378,139
Number of Sequences: 28952
Number of extensions: 314055
Number of successful extensions: 1011
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 973
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1007
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1584903024
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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