BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30845 (756 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g33620.1 68417.m04775 Ulp1 protease family protein low simila... 55 4e-08 At1g09730.1 68414.m01092 Ulp1 protease family protein low simila... 52 5e-07 At1g10570.2 68414.m01191 Ulp1 protease family protein contains P... 41 8e-04 At1g10570.1 68414.m01190 Ulp1 protease family protein contains P... 41 8e-04 At3g48480.1 68416.m05292 hypothetical protein 34 0.12 At4g15880.1 68417.m02413 Ulp1 protease family protein contains P... 32 0.47 At1g29110.1 68414.m03563 cysteine proteinase, putative contains ... 31 0.62 At5g60190.1 68418.m07545 Ulp1 protease family protein low simila... 31 1.1 At1g60220.1 68414.m06782 Ulp1 protease family protein contains P... 30 1.4 At5g15920.1 68418.m01862 structural maintenance of chromosomes (... 30 1.9 At2g27980.1 68415.m03391 expressed protein 30 1.9 At4g00690.1 68417.m00094 Ulp1 protease family protein similar to... 29 2.5 At2g12900.1 68415.m01408 hypothetical protein similar to transcr... 29 3.3 At1g09010.1 68414.m01005 glycoside hydrolase family 2 protein lo... 29 3.3 At1g14380.2 68414.m01705 calmodulin-binding family protein conta... 29 4.4 At1g14380.1 68414.m01704 calmodulin-binding family protein conta... 29 4.4 At4g09950.1 68417.m01628 avirulence-responsive family protein / ... 28 5.8 At1g73870.1 68414.m08554 zinc finger (B-box type) family protein 28 5.8 At4g29040.1 68417.m04153 26S proteasome AAA-ATPase subunit (RPT2... 28 7.7 At2g20140.1 68415.m02353 26S protease regulatory complex subunit... 28 7.7 >At4g33620.1 68417.m04775 Ulp1 protease family protein low similarity to SP|Q9BQF6 SUMO-1-specific protease 2 (EC 3.4.22.-) (Sentrin-specific protease SENP7) {Homo sapiens}; contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain Length = 783 Score = 55.2 bits (127), Expect = 4e-08 Identities = 45/159 (28%), Positives = 70/159 (44%), Gaps = 13/159 (8%) Frame = +2 Query: 104 PIKQPCILIFDSLAGASRSRVVATLRDYLTCEYHVKVSPLKIFNK--DNIKGSCLKIPQQ 277 P + PCIL DS+ G+ + ++ YL E+ + + N++ L++PQQ Sbjct: 429 PQRVPCILHLDSIKGSHKGGLINIFPSYLREEWKARHENTTNDSSRAPNMQSISLELPQQ 488 Query: 278 NNFTDCGLYLLQYVEQFFKDPITDYTLPI-----KQLT-NWFDEIVVTRKREEISNLLKS 439 N DCGL+LL Y++ F + + LT NWF + KR I LL + Sbjct: 489 ENSFDCGLFLLHYLDLFVAQAPAKFNPSLISRSANFLTRNWFPAKEASLKRRNILELLYN 548 Query: 440 LMNKYNP-----DSHLTLPDITFPTLNGKLIESEDNEEC 541 L ++P +S P N + ESE+ EC Sbjct: 549 LHKGHDPSILPANSKSEPPHCGVSNRNDQETESENVIEC 587 >At1g09730.1 68414.m01092 Ulp1 protease family protein low similarity to SP|Q9GZR1 SUMO-1-specific protease 1 (EC 3.4.22.-) (Sentrin-specific protease SENP6) (Protease FKSG6) {Homo sapiens}; contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain Length = 984 Score = 51.6 bits (118), Expect = 5e-07 Identities = 39/121 (32%), Positives = 56/121 (46%), Gaps = 9/121 (7%) Frame = +2 Query: 110 KQPCILIFDSLAGASRSRVVATLRDYLTCEY---HVKVSPLKIFNKDNIKGSCLKIPQQN 280 K PCIL DS+ G S + + ++ YL E+ H + S N++ L++PQQ Sbjct: 567 KVPCILHMDSIKG-SHAGLKNLVQTYLCEEWKERHKETSDDISSRFMNLRFVSLELPQQE 625 Query: 281 NFTDCGLYLLQYVEQFFKD------PITDYTLPIKQLTNWFDEIVVTRKREEISNLLKSL 442 N DCGL+LL Y+E F + P Y NWF + KR I L+ L Sbjct: 626 NSFDCGLFLLHYLELFLAEAPLNFSPFKIYNASNFLYLNWFPPAEASLKRTLIQKLIFEL 685 Query: 443 M 445 + Sbjct: 686 L 686 >At1g10570.2 68414.m01191 Ulp1 protease family protein contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain; similar to EST gb|N96456 Length = 570 Score = 41.1 bits (92), Expect = 8e-04 Identities = 23/68 (33%), Positives = 37/68 (54%), Gaps = 2/68 (2%) Frame = +2 Query: 260 LKIPQQNNFTDCGLYLLQYVEQFFKDPITDYTL-PIKQL-TNWFDEIVVTRKREEISNLL 433 +++PQQ N DCGL+LL ++ +F ++ TL +K + WF + R +I N+L Sbjct: 500 VQVPQQKNDFDCGLFLLFFIRRFIEEAPQRLTLQDLKMIHKKWFKPEEASALRIKIWNIL 559 Query: 434 KSLMNKYN 457 L K N Sbjct: 560 VDLFRKGN 567 >At1g10570.1 68414.m01190 Ulp1 protease family protein contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain; similar to EST gb|N96456 Length = 571 Score = 41.1 bits (92), Expect = 8e-04 Identities = 23/68 (33%), Positives = 37/68 (54%), Gaps = 2/68 (2%) Frame = +2 Query: 260 LKIPQQNNFTDCGLYLLQYVEQFFKDPITDYTL-PIKQL-TNWFDEIVVTRKREEISNLL 433 +++PQQ N DCGL+LL ++ +F ++ TL +K + WF + R +I N+L Sbjct: 501 VQVPQQKNDFDCGLFLLFFIRRFIEEAPQRLTLQDLKMIHKKWFKPEEASALRIKIWNIL 560 Query: 434 KSLMNKYN 457 L K N Sbjct: 561 VDLFRKGN 568 >At3g48480.1 68416.m05292 hypothetical protein Length = 169 Score = 33.9 bits (74), Expect = 0.12 Identities = 15/58 (25%), Positives = 31/58 (53%), Gaps = 3/58 (5%) Frame = +2 Query: 266 IPQQNNFTDCGLYLLQYVEQFFKDPITDYT---LPIKQLTNWFDEIVVTRKREEISNL 430 +PQQ N +CG ++L Y+ +F +D ++ +P +WF + + +E+ +L Sbjct: 108 VPQQTNDVECGSFVLYYIHRFIEDAPENFNVEDMPYFLKEDWFSHKDLEKFCDELHSL 165 >At4g15880.1 68417.m02413 Ulp1 protease family protein contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain; low similarity to sentrin/SUMO-specific protease [Homo sapiens] GI:6906859; identical to cDNA hypothetical protein, partial (1189 bp) GI:2326349 Length = 489 Score = 31.9 bits (69), Expect = 0.47 Identities = 22/69 (31%), Positives = 34/69 (49%) Frame = +2 Query: 122 ILIFDSLAGASRSRVVATLRDYLTCEYHVKVSPLKIFNKDNIKGSCLKIPQQNNFTDCGL 301 +L DSL G ++ L Y+ E + K S KI +PQQ N DCG+ Sbjct: 393 LLYLDSLNGVD-PMILNALAKYMGDEANEK-SGKKIDANSWDMEFVEDLPQQKNGYDCGM 450 Query: 302 YLLQYVEQF 328 ++L+Y++ F Sbjct: 451 FMLKYIDFF 459 Score = 30.3 bits (65), Expect = 1.4 Identities = 12/23 (52%), Positives = 15/23 (65%) Frame = -3 Query: 232 EYFERRYFNMVFTSKIVSQSGYN 164 +Y + YFN F K+VS SGYN Sbjct: 326 KYLKCHYFNTFFYKKLVSDSGYN 348 >At1g29110.1 68414.m03563 cysteine proteinase, putative contains similarity to cysteine protease SPCP1 GI:13491750 from [Ipomoea batatas] Length = 334 Score = 31.5 bits (68), Expect = 0.62 Identities = 27/116 (23%), Positives = 52/116 (44%), Gaps = 4/116 (3%) Frame = +2 Query: 152 SRSRVVATLRDYLTCEYHVK--VSPLKIFNKDNIKGSCLKIPQQNNFTDCGLYLLQYVEQ 325 S++R TL + +YH + +++ ++ K LK+ ++N L+++E Sbjct: 21 SQARPHVTLNEQSIVDYHQQWMTQFSRVYKDESEKEMRLKVFKKN---------LKFIEN 71 Query: 326 FFKDPITDYTLPIKQLTNW-FDEIVVTRKREEIS-NLLKSLMNKYNPDSHLTLPDI 487 F YTL + + T+W +E + T ++ L L NK P + + DI Sbjct: 72 FNNMGNQSYTLGVNEFTDWKTEEFLATHTGLRVNVTSLSELFNKTKPSRNWNMSDI 127 >At5g60190.1 68418.m07545 Ulp1 protease family protein low similarity to sentrin/SUMO-specific protease [Homo sapiens] GI:6906859; contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain Length = 226 Score = 30.7 bits (66), Expect = 1.1 Identities = 27/86 (31%), Positives = 38/86 (44%), Gaps = 6/86 (6%) Frame = +2 Query: 212 VSPLKIFNKDNIKGSCLKIPQQNNFTDCGLYLLQYVEQFFKDPITDYTLPIKQLTNWFDE 391 VSP + N D C PQQ N DCG++LL + + +L WF Sbjct: 140 VSPF-VSNGDASYKECTDTPQQKNGYDCGVFLLATARVICEWFSSGGMKNRDEL--WFAN 196 Query: 392 I------VVTRKREEISNLLKSLMNK 451 + +V REEI L+K LM++ Sbjct: 197 VKETVPDLVNHLREEILALIKKLMSE 222 >At1g60220.1 68414.m06782 Ulp1 protease family protein contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain Length = 604 Score = 30.3 bits (65), Expect = 1.4 Identities = 10/31 (32%), Positives = 22/31 (70%) Frame = +2 Query: 245 IKGSCLKIPQQNNFTDCGLYLLQYVEQFFKD 337 I + +++PQQ N DCG ++L ++++F ++ Sbjct: 529 ISEAVVQVPQQKNDFDCGPFVLFFIKRFIEE 559 >At5g15920.1 68418.m01862 structural maintenance of chromosomes (SMC) family protein (MSS2) similar to SMC-related protein MSS2 [Arabidopsis thaliana] GI:9965743; contains Pfam profiles PF02483: SMC family C-terminal domain, PF02463: RecF/RecN/SMC N terminal domain Length = 1053 Score = 29.9 bits (64), Expect = 1.9 Identities = 16/53 (30%), Positives = 25/53 (47%) Frame = +2 Query: 497 TLNGKLIESEDNEECLEGERGNQNSIKKSEKDLETATLTFVKQIPSELVKRSF 655 ++NGK + EDNE+ L +R KD+E A +K + + R F Sbjct: 384 SINGKKNQKEDNEKLLSQKRYTLRQCVDKLKDMENANNKLLKALANSGADRIF 436 >At2g27980.1 68415.m03391 expressed protein Length = 1037 Score = 29.9 bits (64), Expect = 1.9 Identities = 18/53 (33%), Positives = 29/53 (54%), Gaps = 6/53 (11%) Frame = +2 Query: 395 VVTRKREEISN-LLKSLMNKYNPDS-----HLTLPDITFPTLNGKLIESEDNE 535 ++ R E++SN +L L K P+ + T PDI + L+GKL S+D + Sbjct: 817 LIVRGEEKLSNNILNFLRKKEQPNEENCPDYKTTPDIRWRVLSGKLTSSDDTK 869 >At4g00690.1 68417.m00094 Ulp1 protease family protein similar to SUMO-1/Smt3-specific isopeptidase 2 [Mus musculus] GI:16118473, sentrin/SUMO-specific protease [Homo sapiens] GI:6906859; contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain Length = 242 Score = 29.5 bits (63), Expect = 2.5 Identities = 12/23 (52%), Positives = 16/23 (69%) Frame = -3 Query: 232 EYFERRYFNMVFTSKIVSQSGYN 164 +YF+ +FN F K+VS SGYN Sbjct: 71 KYFKCHFFNTFFYVKLVSGSGYN 93 Score = 29.5 bits (63), Expect = 2.5 Identities = 9/18 (50%), Positives = 15/18 (83%) Frame = +2 Query: 269 PQQNNFTDCGLYLLQYVE 322 PQQ N DCG+++L+Y++ Sbjct: 186 PQQQNGYDCGMFMLKYID 203 >At2g12900.1 68415.m01408 hypothetical protein similar to transcription factor(bZIP family) VSF-1 GI:3425907 from [Lycopersicon esculentum] Length = 264 Score = 29.1 bits (62), Expect = 3.3 Identities = 14/29 (48%), Positives = 18/29 (62%) Frame = +2 Query: 170 ATLRDYLTCEYHVKVSPLKIFNKDNIKGS 256 A LRD LT + HV++ LK+ N KGS Sbjct: 190 AQLRDALTEKLHVEIERLKVITISNEKGS 218 >At1g09010.1 68414.m01005 glycoside hydrolase family 2 protein low similarity to mannosidase [gi:5359712] from Cellulomonas fimi Length = 944 Score = 29.1 bits (62), Expect = 3.3 Identities = 14/26 (53%), Positives = 16/26 (61%) Frame = +2 Query: 275 QNNFTDCGLYLLQYVEQFFKDPITDY 352 + NFTD G Y +QY E FFKD Y Sbjct: 520 KGNFTD-GPYEIQYPEDFFKDTYYKY 544 >At1g14380.2 68414.m01705 calmodulin-binding family protein contains Pfam profile PF00612: IQ calmodulin-binding motif Length = 602 Score = 28.7 bits (61), Expect = 4.4 Identities = 21/68 (30%), Positives = 32/68 (47%), Gaps = 1/68 (1%) Frame = +2 Query: 545 EGERGNQNSIKKSEKDLETATLTFVKQIPSELVKR-SFGDPSDGTIVRKTIRIASDIENR 721 E ++ N NS K E + L + PS +KR S + S +R + DI + Sbjct: 310 ESDKANHNSRKSRSGSKEGSPLEIKDEKPSPSLKRSSLSNGSKKATLRSAEKKKKDIPDS 369 Query: 722 SLVQMKPE 745 S VQ++PE Sbjct: 370 S-VQIQPE 376 >At1g14380.1 68414.m01704 calmodulin-binding family protein contains Pfam profile PF00612: IQ calmodulin-binding motif Length = 664 Score = 28.7 bits (61), Expect = 4.4 Identities = 21/68 (30%), Positives = 32/68 (47%), Gaps = 1/68 (1%) Frame = +2 Query: 545 EGERGNQNSIKKSEKDLETATLTFVKQIPSELVKR-SFGDPSDGTIVRKTIRIASDIENR 721 E ++ N NS K E + L + PS +KR S + S +R + DI + Sbjct: 310 ESDKANHNSRKSRSGSKEGSPLEIKDEKPSPSLKRSSLSNGSKKATLRSAEKKKKDIPDS 369 Query: 722 SLVQMKPE 745 S VQ++PE Sbjct: 370 S-VQIQPE 376 >At4g09950.1 68417.m01628 avirulence-responsive family protein / avirulence induced gene (AIG1) family protein similar to AIG1 protein SP:P54120 [Arabidopsis thaliana]; contains Pfam PF04548: AIG1 family; Length = 336 Score = 28.3 bits (60), Expect = 5.8 Identities = 41/188 (21%), Positives = 79/188 (42%), Gaps = 7/188 (3%) Frame = +2 Query: 119 CILIFDSLAGASRSRVVATLRDYLTCEYHVKVSPLKIFNKDNIKGSCLKIPQQNNFTDCG 298 C+L+ + G +V +LR+ LT E + LKI I + + + +CG Sbjct: 94 CLLL--AKGGIDAVLLVFSLRNRLTEEEQSTLRTLKILFGSQIVDYIIVVFTNEDALECG 151 Query: 299 LYLLQYVEQF--FKDPITDYTLPIKQLTNWFDEIVVTRKRE--EISNLLKSLMNKYNPDS 466 L Y+E F++ + + N ++ V + R+ ++ NL++ + K N S Sbjct: 152 ETLDDYLEDCPEFQEILEECDDRKVLFDNSYNAPVSKKDRQVHDLLNLVEQISKKNNGKS 211 Query: 467 HLTLPDITFPTLNGKLIESEDNEECLEGERGNQNSIKKSEKDLETA---TLTFVKQIPSE 637 ++ + E + E ++G Q I + +K+LE + L +K+ S Sbjct: 212 YMADLSHELRENEATIKEKQKQIEEMKGWSSKQ-EISQMKKELEKSHNEMLEGIKEKISN 270 Query: 638 LVKRSFGD 661 +K S D Sbjct: 271 QLKESLED 278 >At1g73870.1 68414.m08554 zinc finger (B-box type) family protein Length = 392 Score = 28.3 bits (60), Expect = 5.8 Identities = 21/91 (23%), Positives = 44/91 (48%), Gaps = 1/91 (1%) Frame = +2 Query: 476 LPD-ITFPTLNGKLIESEDNEECLEGERGNQNSIKKSEKDLETATLTFVKQIPSELVKRS 652 +PD + T NG+L E E + + + G + + DLE T+ + E + Sbjct: 160 VPDEVNNTTTNGELGEVE---KAIMDDEGFMGFVPL-DMDLEDLTMDVESLLEEEQLCLG 215 Query: 653 FGDPSDGTIVRKTIRIASDIENRSLVQMKPE 745 F +P+D ++++ ++ +I + L ++K E Sbjct: 216 FKEPNDVGVIKEENKVGFEINCKDLKRVKDE 246 >At4g29040.1 68417.m04153 26S proteasome AAA-ATPase subunit (RPT2a) almost identical to 26S proteasome AAA-ATPase subunit RPT2a (GI:6652880) {Arabidopsis thaliana}; Drosophila melanogaster 26S proteasome subunit 4 ATPase, PID:g1066065 Length = 443 Score = 27.9 bits (59), Expect = 7.7 Identities = 16/57 (28%), Positives = 32/57 (56%) Frame = +2 Query: 542 LEGERGNQNSIKKSEKDLETATLTFVKQIPSELVKRSFGDPSDGTIVRKTIRIASDI 712 L GE G ++ ++ + + TF++ + SEL+++ GD +VR+ R+A D+ Sbjct: 227 LYGEPGTGKTLL-AKAVANSTSATFLRVVGSELIQKYLGD--GPKLVRELFRVADDL 280 >At2g20140.1 68415.m02353 26S protease regulatory complex subunit 4, putative similar to Swiss-Prot:P48601 26S protease regulatory subunit 4 (P26S4) [Drosophila melanogaster] Length = 443 Score = 27.9 bits (59), Expect = 7.7 Identities = 16/57 (28%), Positives = 32/57 (56%) Frame = +2 Query: 542 LEGERGNQNSIKKSEKDLETATLTFVKQIPSELVKRSFGDPSDGTIVRKTIRIASDI 712 L GE G ++ ++ + + TF++ + SEL+++ GD +VR+ R+A D+ Sbjct: 227 LYGEPGTGKTLL-AKAVANSTSATFLRVVGSELIQKYLGD--GPKLVRELFRVADDL 280 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,027,351 Number of Sequences: 28952 Number of extensions: 264481 Number of successful extensions: 883 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 839 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 879 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1682736544 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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