SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30845
         (756 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g33620.1 68417.m04775 Ulp1 protease family protein low simila...    55   4e-08
At1g09730.1 68414.m01092 Ulp1 protease family protein low simila...    52   5e-07
At1g10570.2 68414.m01191 Ulp1 protease family protein contains P...    41   8e-04
At1g10570.1 68414.m01190 Ulp1 protease family protein contains P...    41   8e-04
At3g48480.1 68416.m05292 hypothetical protein                          34   0.12 
At4g15880.1 68417.m02413 Ulp1 protease family protein contains P...    32   0.47 
At1g29110.1 68414.m03563 cysteine proteinase, putative contains ...    31   0.62 
At5g60190.1 68418.m07545 Ulp1 protease family protein low simila...    31   1.1  
At1g60220.1 68414.m06782 Ulp1 protease family protein contains P...    30   1.4  
At5g15920.1 68418.m01862 structural maintenance of chromosomes (...    30   1.9  
At2g27980.1 68415.m03391 expressed protein                             30   1.9  
At4g00690.1 68417.m00094 Ulp1 protease family protein similar to...    29   2.5  
At2g12900.1 68415.m01408 hypothetical protein similar to transcr...    29   3.3  
At1g09010.1 68414.m01005 glycoside hydrolase family 2 protein lo...    29   3.3  
At1g14380.2 68414.m01705 calmodulin-binding family protein conta...    29   4.4  
At1g14380.1 68414.m01704 calmodulin-binding family protein conta...    29   4.4  
At4g09950.1 68417.m01628 avirulence-responsive family protein / ...    28   5.8  
At1g73870.1 68414.m08554 zinc finger (B-box type) family protein       28   5.8  
At4g29040.1 68417.m04153 26S proteasome AAA-ATPase subunit (RPT2...    28   7.7  
At2g20140.1 68415.m02353 26S protease regulatory complex subunit...    28   7.7  

>At4g33620.1 68417.m04775 Ulp1 protease family protein low
           similarity to SP|Q9BQF6 SUMO-1-specific protease 2 (EC
           3.4.22.-) (Sentrin-specific protease SENP7) {Homo
           sapiens}; contains Pfam profile PF02902: Ulp1 protease
           family, C-terminal catalytic domain
          Length = 783

 Score = 55.2 bits (127), Expect = 4e-08
 Identities = 45/159 (28%), Positives = 70/159 (44%), Gaps = 13/159 (8%)
 Frame = +2

Query: 104 PIKQPCILIFDSLAGASRSRVVATLRDYLTCEYHVKVSPLKIFNK--DNIKGSCLKIPQQ 277
           P + PCIL  DS+ G+ +  ++     YL  E+  +       +    N++   L++PQQ
Sbjct: 429 PQRVPCILHLDSIKGSHKGGLINIFPSYLREEWKARHENTTNDSSRAPNMQSISLELPQQ 488

Query: 278 NNFTDCGLYLLQYVEQFFKDPITDYTLPI-----KQLT-NWFDEIVVTRKREEISNLLKS 439
            N  DCGL+LL Y++ F       +   +       LT NWF     + KR  I  LL +
Sbjct: 489 ENSFDCGLFLLHYLDLFVAQAPAKFNPSLISRSANFLTRNWFPAKEASLKRRNILELLYN 548

Query: 440 LMNKYNP-----DSHLTLPDITFPTLNGKLIESEDNEEC 541
           L   ++P     +S    P       N +  ESE+  EC
Sbjct: 549 LHKGHDPSILPANSKSEPPHCGVSNRNDQETESENVIEC 587


>At1g09730.1 68414.m01092 Ulp1 protease family protein low
           similarity to SP|Q9GZR1 SUMO-1-specific protease 1 (EC
           3.4.22.-) (Sentrin-specific protease SENP6) (Protease
           FKSG6) {Homo sapiens}; contains Pfam profile PF02902:
           Ulp1 protease family, C-terminal catalytic domain
          Length = 984

 Score = 51.6 bits (118), Expect = 5e-07
 Identities = 39/121 (32%), Positives = 56/121 (46%), Gaps = 9/121 (7%)
 Frame = +2

Query: 110 KQPCILIFDSLAGASRSRVVATLRDYLTCEY---HVKVSPLKIFNKDNIKGSCLKIPQQN 280
           K PCIL  DS+ G S + +   ++ YL  E+   H + S        N++   L++PQQ 
Sbjct: 567 KVPCILHMDSIKG-SHAGLKNLVQTYLCEEWKERHKETSDDISSRFMNLRFVSLELPQQE 625

Query: 281 NFTDCGLYLLQYVEQFFKD------PITDYTLPIKQLTNWFDEIVVTRKREEISNLLKSL 442
           N  DCGL+LL Y+E F  +      P   Y        NWF     + KR  I  L+  L
Sbjct: 626 NSFDCGLFLLHYLELFLAEAPLNFSPFKIYNASNFLYLNWFPPAEASLKRTLIQKLIFEL 685

Query: 443 M 445
           +
Sbjct: 686 L 686


>At1g10570.2 68414.m01191 Ulp1 protease family protein contains Pfam
           profile PF02902: Ulp1 protease family, C-terminal
           catalytic domain; similar to EST gb|N96456
          Length = 570

 Score = 41.1 bits (92), Expect = 8e-04
 Identities = 23/68 (33%), Positives = 37/68 (54%), Gaps = 2/68 (2%)
 Frame = +2

Query: 260 LKIPQQNNFTDCGLYLLQYVEQFFKDPITDYTL-PIKQL-TNWFDEIVVTRKREEISNLL 433
           +++PQQ N  DCGL+LL ++ +F ++     TL  +K +   WF     +  R +I N+L
Sbjct: 500 VQVPQQKNDFDCGLFLLFFIRRFIEEAPQRLTLQDLKMIHKKWFKPEEASALRIKIWNIL 559

Query: 434 KSLMNKYN 457
             L  K N
Sbjct: 560 VDLFRKGN 567


>At1g10570.1 68414.m01190 Ulp1 protease family protein contains Pfam
           profile PF02902: Ulp1 protease family, C-terminal
           catalytic domain; similar to EST gb|N96456
          Length = 571

 Score = 41.1 bits (92), Expect = 8e-04
 Identities = 23/68 (33%), Positives = 37/68 (54%), Gaps = 2/68 (2%)
 Frame = +2

Query: 260 LKIPQQNNFTDCGLYLLQYVEQFFKDPITDYTL-PIKQL-TNWFDEIVVTRKREEISNLL 433
           +++PQQ N  DCGL+LL ++ +F ++     TL  +K +   WF     +  R +I N+L
Sbjct: 501 VQVPQQKNDFDCGLFLLFFIRRFIEEAPQRLTLQDLKMIHKKWFKPEEASALRIKIWNIL 560

Query: 434 KSLMNKYN 457
             L  K N
Sbjct: 561 VDLFRKGN 568


>At3g48480.1 68416.m05292 hypothetical protein
          Length = 169

 Score = 33.9 bits (74), Expect = 0.12
 Identities = 15/58 (25%), Positives = 31/58 (53%), Gaps = 3/58 (5%)
 Frame = +2

Query: 266 IPQQNNFTDCGLYLLQYVEQFFKDPITDYT---LPIKQLTNWFDEIVVTRKREEISNL 430
           +PQQ N  +CG ++L Y+ +F +D   ++    +P     +WF    + +  +E+ +L
Sbjct: 108 VPQQTNDVECGSFVLYYIHRFIEDAPENFNVEDMPYFLKEDWFSHKDLEKFCDELHSL 165


>At4g15880.1 68417.m02413 Ulp1 protease family protein contains Pfam
           profile PF02902: Ulp1 protease family, C-terminal
           catalytic domain; low similarity to
           sentrin/SUMO-specific protease [Homo sapiens]
           GI:6906859;  identical to cDNA hypothetical protein,
           partial (1189 bp) GI:2326349
          Length = 489

 Score = 31.9 bits (69), Expect = 0.47
 Identities = 22/69 (31%), Positives = 34/69 (49%)
 Frame = +2

Query: 122 ILIFDSLAGASRSRVVATLRDYLTCEYHVKVSPLKIFNKDNIKGSCLKIPQQNNFTDCGL 301
           +L  DSL G     ++  L  Y+  E + K S  KI            +PQQ N  DCG+
Sbjct: 393 LLYLDSLNGVD-PMILNALAKYMGDEANEK-SGKKIDANSWDMEFVEDLPQQKNGYDCGM 450

Query: 302 YLLQYVEQF 328
           ++L+Y++ F
Sbjct: 451 FMLKYIDFF 459



 Score = 30.3 bits (65), Expect = 1.4
 Identities = 12/23 (52%), Positives = 15/23 (65%)
 Frame = -3

Query: 232 EYFERRYFNMVFTSKIVSQSGYN 164
           +Y +  YFN  F  K+VS SGYN
Sbjct: 326 KYLKCHYFNTFFYKKLVSDSGYN 348


>At1g29110.1 68414.m03563 cysteine proteinase, putative contains
           similarity to cysteine protease SPCP1 GI:13491750 from
           [Ipomoea batatas]
          Length = 334

 Score = 31.5 bits (68), Expect = 0.62
 Identities = 27/116 (23%), Positives = 52/116 (44%), Gaps = 4/116 (3%)
 Frame = +2

Query: 152 SRSRVVATLRDYLTCEYHVK--VSPLKIFNKDNIKGSCLKIPQQNNFTDCGLYLLQYVEQ 325
           S++R   TL +    +YH +      +++  ++ K   LK+ ++N         L+++E 
Sbjct: 21  SQARPHVTLNEQSIVDYHQQWMTQFSRVYKDESEKEMRLKVFKKN---------LKFIEN 71

Query: 326 FFKDPITDYTLPIKQLTNW-FDEIVVTRKREEIS-NLLKSLMNKYNPDSHLTLPDI 487
           F       YTL + + T+W  +E + T     ++   L  L NK  P  +  + DI
Sbjct: 72  FNNMGNQSYTLGVNEFTDWKTEEFLATHTGLRVNVTSLSELFNKTKPSRNWNMSDI 127


>At5g60190.1 68418.m07545 Ulp1 protease family protein low
           similarity to sentrin/SUMO-specific protease [Homo
           sapiens] GI:6906859; contains Pfam profile PF02902: Ulp1
           protease family, C-terminal catalytic domain
          Length = 226

 Score = 30.7 bits (66), Expect = 1.1
 Identities = 27/86 (31%), Positives = 38/86 (44%), Gaps = 6/86 (6%)
 Frame = +2

Query: 212 VSPLKIFNKDNIKGSCLKIPQQNNFTDCGLYLLQYVEQFFKDPITDYTLPIKQLTNWFDE 391
           VSP  + N D     C   PQQ N  DCG++LL       +   +       +L  WF  
Sbjct: 140 VSPF-VSNGDASYKECTDTPQQKNGYDCGVFLLATARVICEWFSSGGMKNRDEL--WFAN 196

Query: 392 I------VVTRKREEISNLLKSLMNK 451
           +      +V   REEI  L+K LM++
Sbjct: 197 VKETVPDLVNHLREEILALIKKLMSE 222


>At1g60220.1 68414.m06782 Ulp1 protease family protein contains Pfam
           profile PF02902: Ulp1 protease family, C-terminal
           catalytic domain
          Length = 604

 Score = 30.3 bits (65), Expect = 1.4
 Identities = 10/31 (32%), Positives = 22/31 (70%)
 Frame = +2

Query: 245 IKGSCLKIPQQNNFTDCGLYLLQYVEQFFKD 337
           I  + +++PQQ N  DCG ++L ++++F ++
Sbjct: 529 ISEAVVQVPQQKNDFDCGPFVLFFIKRFIEE 559


>At5g15920.1 68418.m01862 structural maintenance of chromosomes
           (SMC) family protein (MSS2) similar to SMC-related
           protein MSS2 [Arabidopsis thaliana] GI:9965743; contains
           Pfam profiles PF02483: SMC family C-terminal domain,
           PF02463: RecF/RecN/SMC N terminal domain
          Length = 1053

 Score = 29.9 bits (64), Expect = 1.9
 Identities = 16/53 (30%), Positives = 25/53 (47%)
 Frame = +2

Query: 497 TLNGKLIESEDNEECLEGERGNQNSIKKSEKDLETATLTFVKQIPSELVKRSF 655
           ++NGK  + EDNE+ L  +R          KD+E A    +K + +    R F
Sbjct: 384 SINGKKNQKEDNEKLLSQKRYTLRQCVDKLKDMENANNKLLKALANSGADRIF 436


>At2g27980.1 68415.m03391 expressed protein
          Length = 1037

 Score = 29.9 bits (64), Expect = 1.9
 Identities = 18/53 (33%), Positives = 29/53 (54%), Gaps = 6/53 (11%)
 Frame = +2

Query: 395 VVTRKREEISN-LLKSLMNKYNPDS-----HLTLPDITFPTLNGKLIESEDNE 535
           ++ R  E++SN +L  L  K  P+      + T PDI +  L+GKL  S+D +
Sbjct: 817 LIVRGEEKLSNNILNFLRKKEQPNEENCPDYKTTPDIRWRVLSGKLTSSDDTK 869


>At4g00690.1 68417.m00094 Ulp1 protease family protein similar to
           SUMO-1/Smt3-specific isopeptidase 2 [Mus musculus]
           GI:16118473, sentrin/SUMO-specific protease [Homo
           sapiens] GI:6906859; contains Pfam profile PF02902: Ulp1
           protease family, C-terminal catalytic domain
          Length = 242

 Score = 29.5 bits (63), Expect = 2.5
 Identities = 12/23 (52%), Positives = 16/23 (69%)
 Frame = -3

Query: 232 EYFERRYFNMVFTSKIVSQSGYN 164
           +YF+  +FN  F  K+VS SGYN
Sbjct: 71  KYFKCHFFNTFFYVKLVSGSGYN 93



 Score = 29.5 bits (63), Expect = 2.5
 Identities = 9/18 (50%), Positives = 15/18 (83%)
 Frame = +2

Query: 269 PQQNNFTDCGLYLLQYVE 322
           PQQ N  DCG+++L+Y++
Sbjct: 186 PQQQNGYDCGMFMLKYID 203


>At2g12900.1 68415.m01408 hypothetical protein similar to
           transcription factor(bZIP family) VSF-1 GI:3425907 from
           [Lycopersicon esculentum]
          Length = 264

 Score = 29.1 bits (62), Expect = 3.3
 Identities = 14/29 (48%), Positives = 18/29 (62%)
 Frame = +2

Query: 170 ATLRDYLTCEYHVKVSPLKIFNKDNIKGS 256
           A LRD LT + HV++  LK+    N KGS
Sbjct: 190 AQLRDALTEKLHVEIERLKVITISNEKGS 218


>At1g09010.1 68414.m01005 glycoside hydrolase family 2 protein low
           similarity to mannosidase [gi:5359712] from Cellulomonas
           fimi
          Length = 944

 Score = 29.1 bits (62), Expect = 3.3
 Identities = 14/26 (53%), Positives = 16/26 (61%)
 Frame = +2

Query: 275 QNNFTDCGLYLLQYVEQFFKDPITDY 352
           + NFTD G Y +QY E FFKD    Y
Sbjct: 520 KGNFTD-GPYEIQYPEDFFKDTYYKY 544


>At1g14380.2 68414.m01705 calmodulin-binding family protein contains
           Pfam profile PF00612: IQ calmodulin-binding motif
          Length = 602

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 21/68 (30%), Positives = 32/68 (47%), Gaps = 1/68 (1%)
 Frame = +2

Query: 545 EGERGNQNSIKKSEKDLETATLTFVKQIPSELVKR-SFGDPSDGTIVRKTIRIASDIENR 721
           E ++ N NS K      E + L    + PS  +KR S  + S    +R   +   DI + 
Sbjct: 310 ESDKANHNSRKSRSGSKEGSPLEIKDEKPSPSLKRSSLSNGSKKATLRSAEKKKKDIPDS 369

Query: 722 SLVQMKPE 745
           S VQ++PE
Sbjct: 370 S-VQIQPE 376


>At1g14380.1 68414.m01704 calmodulin-binding family protein contains
           Pfam profile PF00612: IQ calmodulin-binding motif
          Length = 664

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 21/68 (30%), Positives = 32/68 (47%), Gaps = 1/68 (1%)
 Frame = +2

Query: 545 EGERGNQNSIKKSEKDLETATLTFVKQIPSELVKR-SFGDPSDGTIVRKTIRIASDIENR 721
           E ++ N NS K      E + L    + PS  +KR S  + S    +R   +   DI + 
Sbjct: 310 ESDKANHNSRKSRSGSKEGSPLEIKDEKPSPSLKRSSLSNGSKKATLRSAEKKKKDIPDS 369

Query: 722 SLVQMKPE 745
           S VQ++PE
Sbjct: 370 S-VQIQPE 376


>At4g09950.1 68417.m01628 avirulence-responsive family protein /
           avirulence induced gene (AIG1) family protein similar to
           AIG1 protein SP:P54120 [Arabidopsis thaliana]; contains
           Pfam PF04548: AIG1 family;
          Length = 336

 Score = 28.3 bits (60), Expect = 5.8
 Identities = 41/188 (21%), Positives = 79/188 (42%), Gaps = 7/188 (3%)
 Frame = +2

Query: 119 CILIFDSLAGASRSRVVATLRDYLTCEYHVKVSPLKIFNKDNIKGSCLKIPQQNNFTDCG 298
           C+L+  +  G     +V +LR+ LT E    +  LKI     I    + +    +  +CG
Sbjct: 94  CLLL--AKGGIDAVLLVFSLRNRLTEEEQSTLRTLKILFGSQIVDYIIVVFTNEDALECG 151

Query: 299 LYLLQYVEQF--FKDPITDYTLPIKQLTNWFDEIVVTRKRE--EISNLLKSLMNKYNPDS 466
             L  Y+E    F++ + +         N ++  V  + R+  ++ NL++ +  K N  S
Sbjct: 152 ETLDDYLEDCPEFQEILEECDDRKVLFDNSYNAPVSKKDRQVHDLLNLVEQISKKNNGKS 211

Query: 467 HLTLPDITFPTLNGKLIESEDNEECLEGERGNQNSIKKSEKDLETA---TLTFVKQIPSE 637
           ++             + E +   E ++G    Q  I + +K+LE +    L  +K+  S 
Sbjct: 212 YMADLSHELRENEATIKEKQKQIEEMKGWSSKQ-EISQMKKELEKSHNEMLEGIKEKISN 270

Query: 638 LVKRSFGD 661
            +K S  D
Sbjct: 271 QLKESLED 278


>At1g73870.1 68414.m08554 zinc finger (B-box type) family protein
          Length = 392

 Score = 28.3 bits (60), Expect = 5.8
 Identities = 21/91 (23%), Positives = 44/91 (48%), Gaps = 1/91 (1%)
 Frame = +2

Query: 476 LPD-ITFPTLNGKLIESEDNEECLEGERGNQNSIKKSEKDLETATLTFVKQIPSELVKRS 652
           +PD +   T NG+L E E   + +  + G    +   + DLE  T+     +  E +   
Sbjct: 160 VPDEVNNTTTNGELGEVE---KAIMDDEGFMGFVPL-DMDLEDLTMDVESLLEEEQLCLG 215

Query: 653 FGDPSDGTIVRKTIRIASDIENRSLVQMKPE 745
           F +P+D  ++++  ++  +I  + L ++K E
Sbjct: 216 FKEPNDVGVIKEENKVGFEINCKDLKRVKDE 246


>At4g29040.1 68417.m04153 26S proteasome AAA-ATPase subunit (RPT2a)
           almost identical to 26S proteasome AAA-ATPase subunit
           RPT2a (GI:6652880) {Arabidopsis thaliana}; Drosophila
           melanogaster 26S proteasome subunit 4 ATPase,
           PID:g1066065
          Length = 443

 Score = 27.9 bits (59), Expect = 7.7
 Identities = 16/57 (28%), Positives = 32/57 (56%)
 Frame = +2

Query: 542 LEGERGNQNSIKKSEKDLETATLTFVKQIPSELVKRSFGDPSDGTIVRKTIRIASDI 712
           L GE G   ++  ++    + + TF++ + SEL+++  GD     +VR+  R+A D+
Sbjct: 227 LYGEPGTGKTLL-AKAVANSTSATFLRVVGSELIQKYLGD--GPKLVRELFRVADDL 280


>At2g20140.1 68415.m02353 26S protease regulatory complex subunit 4,
           putative similar to Swiss-Prot:P48601 26S protease
           regulatory subunit 4 (P26S4) [Drosophila melanogaster]
          Length = 443

 Score = 27.9 bits (59), Expect = 7.7
 Identities = 16/57 (28%), Positives = 32/57 (56%)
 Frame = +2

Query: 542 LEGERGNQNSIKKSEKDLETATLTFVKQIPSELVKRSFGDPSDGTIVRKTIRIASDI 712
           L GE G   ++  ++    + + TF++ + SEL+++  GD     +VR+  R+A D+
Sbjct: 227 LYGEPGTGKTLL-AKAVANSTSATFLRVVGSELIQKYLGD--GPKLVRELFRVADDL 280


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,027,351
Number of Sequences: 28952
Number of extensions: 264481
Number of successful extensions: 883
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 839
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 879
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1682736544
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -