Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30842
(823 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g22930.1 68416.m02889 calmodulin, putative strong similarity ... 95 7e-20
At2g27030.3 68415.m03247 calmodulin-2/3/5 (CAM5) (TCH1) identica... 95 7e-20
At5g21274.1 68418.m02533 calmodulin-6 (CAM6) identical to calmod... 93 2e-19
At3g56800.1 68416.m06317 calmodulin-2/3/5 (CAM3) identical to ca... 93 3e-19
At2g41110.1 68415.m05078 calmodulin-2/3/5 (CAM2) (CAL1) almost i... 93 3e-19
At2g27030.1 68415.m03245 calmodulin-2/3/5 (CAM5) (TCH1) identica... 93 3e-19
At3g43810.1 68416.m04682 calmodulin-7 (CAM7) almost identical to... 92 4e-19
At5g37780.1 68418.m04549 calmodulin-1/4 (CAM1) identical to calm... 91 6e-19
At1g66410.1 68414.m07542 calmodulin-1/4 (CAM4) identical to calm... 91 6e-19
At4g14640.1 68417.m02252 calmodulin-8 (CAM8) identical to calmod... 83 2e-16
At3g51920.1 68416.m05695 calmodulin-9 (CAM9) identical to calmod... 72 4e-13
At1g62820.1 68414.m07092 calmodulin, putative similar to calmodu... 69 5e-12
At1g12310.1 68414.m01423 calmodulin, putative similar to calmodu... 68 7e-12
At2g27030.2 68415.m03246 calmodulin-2/3/5 (CAM5) (TCH1) identica... 66 4e-11
At3g50360.1 68416.m05507 caltractin / centrin identical to caltr... 65 6e-11
At1g76040.2 68414.m08829 calcium-dependent protein kinase, putat... 64 8e-11
At1g76040.1 68414.m08830 calcium-dependent protein kinase, putat... 64 8e-11
At3g03000.1 68416.m00295 calmodulin, putative similar to calmodu... 63 2e-10
At2g41090.1 68415.m05075 calmodulin-like calcium-binding protein... 60 1e-09
At5g23580.1 68418.m02767 calcium-dependent protein kinase 9 (CDP... 60 2e-09
At4g12860.1 68417.m02014 calcium-binding protein, putative simil... 60 2e-09
At2g15680.1 68415.m01795 calmodulin-related protein, putative si... 59 4e-09
At2g41100.1 68415.m05076 touch-responsive protein / calmodulin-r... 58 5e-09
At1g24620.1 68414.m03097 polcalcin, putative / calcium-binding p... 58 9e-09
At5g12480.1 68418.m01466 calmodulin-domain protein kinase isofor... 57 2e-08
At2g41100.2 68415.m05077 touch-responsive protein / calmodulin-r... 54 2e-07
At1g05990.1 68414.m00627 calcium-binding protein, putative stron... 52 5e-07
At1g18890.1 68414.m02351 calcium-dependent protein kinase 1 (CDP... 51 8e-07
At5g37770.1 68418.m04547 touch-responsive protein / calmodulin-r... 50 1e-06
At3g51850.1 68416.m05686 calcium-dependent protein kinase, putat... 50 2e-06
At5g19450.2 68418.m02318 calcium-dependent protein kinase 19 (CD... 50 2e-06
At5g19450.1 68418.m02317 calcium-dependent protein kinase 19 (CD... 50 2e-06
At1g74740.1 68414.m08660 calcium-dependent protein kinase, putat... 50 2e-06
At4g04695.1 68417.m00689 calcium-dependent protein kinase, putat... 49 3e-06
At3g50770.1 68416.m05560 calmodulin-related protein, putative si... 49 3e-06
At2g41410.1 68415.m05110 calmodulin, putative identical to SP|P3... 48 6e-06
At1g66400.1 68414.m07541 calmodulin-related protein, putative si... 47 2e-05
At3g10190.1 68416.m01220 calmodulin, putative similar to calmodu... 46 4e-05
At1g18210.2 68414.m02267 calcium-binding protein, putative simil... 42 5e-04
At1g18210.1 68414.m02266 calcium-binding protein, putative simil... 42 5e-04
At5g49480.1 68418.m06123 sodium-inducible calcium-binding protei... 40 0.002
At5g17470.1 68418.m02050 calmodulin-related protein, putative si... 40 0.002
At4g37010.1 68417.m05243 caltractin, putative / centrin, putativ... 40 0.002
At3g03400.1 68416.m00337 calmodulin-related protein, putative si... 40 0.003
At3g25600.1 68416.m03187 calmodulin, putative similar to calmodu... 39 0.003
At1g73630.1 68414.m08524 calcium-binding protein, putative simil... 39 0.003
At2g36180.1 68415.m04440 calmodulin-related protein, putative si... 38 0.008
At1g76640.1 68414.m08918 calmodulin-related protein, putative si... 38 0.011
At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains... 37 0.014
At5g42380.1 68418.m05160 calmodulin-related protein, putative si... 36 0.032
At5g66210.2 68418.m08341 calcium-dependent protein kinase family... 35 0.057
At5g66210.1 68418.m08340 calcium-dependent protein kinase family... 35 0.057
At4g04710.1 68417.m00692 calcium-dependent protein kinase, putat... 35 0.057
At1g21550.1 68414.m02695 calcium-binding protein, putative conta... 35 0.075
At3g59440.1 68416.m06630 calcium-binding protein, putative simil... 34 0.13
At3g57530.1 68416.m06406 calcium-dependent protein kinase, putat... 34 0.13
At4g03290.1 68417.m00449 calcium-binding protein, putative simil... 33 0.23
At2g44310.1 68415.m05513 calcium-binding EF hand family protein ... 33 0.23
At5g39670.1 68418.m04804 calcium-binding EF hand family protein ... 33 0.30
At3g03410.1 68416.m00339 calmodulin-related protein, putative si... 33 0.30
At1g18530.1 68414.m02312 calmodulin, putative similar to calmodu... 33 0.30
At4g25970.1 68417.m03737 phosphatidylserine decarboxylase, putat... 32 0.40
At3g29000.1 68416.m03624 calcium-binding EF hand family protein ... 32 0.40
At3g47480.1 68416.m05163 calcium-binding EF hand family protein ... 32 0.53
At4g00310.1 68417.m00039 expressed protein 31 0.70
At2g43290.1 68415.m05382 calmodulin-like protein (MSS3) identica... 31 0.70
At4g20780.1 68417.m03017 calcium-binding protein, putative simil... 31 0.92
At3g07490.1 68416.m00893 calcium-binding protein, putative simil... 31 0.92
At4g20190.1 68417.m02952 hypothetical protein 31 1.2
At4g04740.1 68417.m00695 calcium-dependent protein kinase, putat... 31 1.2
At4g02540.1 68417.m00347 DC1 domain-containing protein contains ... 30 1.6
At3g51670.1 68416.m05666 SEC14 cytosolic factor family protein /... 30 1.6
At2g17890.1 68415.m02072 calcium-dependent protein kinase family... 30 1.6
At2g41860.2 68415.m05174 calcium-dependent protein kinase, putat... 30 2.1
At2g41860.1 68415.m05173 calcium-dependent protein kinase, putat... 30 2.1
At5g25630.1 68418.m03050 pentatricopeptide (PPR) repeat-containi... 29 2.8
At5g51600.1 68418.m06397 microtubule associated protein (MAP65/A... 29 3.7
At4g04700.1 68417.m00690 calcium-dependent protein kinase, putat... 29 3.7
At3g43480.1 68416.m04609 hypothetical protein hypothetical prote... 29 3.7
At3g43470.1 68416.m04608 hypothetical protein 29 3.7
At2g34210.1 68415.m04186 KOW domain-containing transcription fac... 29 3.7
At2g15185.1 68415.m01732 zinc knuckle (CCHC-type) protein-relate... 29 3.7
At1g54530.1 68414.m06219 calcium-binding EF hand family protein ... 29 4.9
At1g13800.1 68414.m01620 pentatricopeptide (PPR) repeat-containi... 29 4.9
At3g10300.3 68416.m01236 calcium-binding EF hand family protein ... 28 6.5
At3g10300.2 68416.m01235 calcium-binding EF hand family protein ... 28 6.5
At5g51440.1 68418.m06377 23.5 kDa mitochondrial small heat shock... 28 8.6
At2g19390.1 68415.m02262 expressed protein 28 8.6
>At3g22930.1 68416.m02889 calmodulin, putative strong similarity to
calmodulin 8 GI:5825600 from [Arabidopsis thaliana];
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 173
Score = 94.7 bits (225), Expect = 7e-20
Identities = 50/142 (35%), Positives = 84/142 (59%), Gaps = 6/142 (4%)
Frame = +3
Query: 171 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 338
+Q+Q+ EFKEAF L D D DG I ++L SL + +E+EL +M+ E +G I
Sbjct: 29 TQEQIMEFKEAFCLFDKDGDGCITADELATVIRSLDQNPTEQELQDMITEIDSDGNGTIE 88
Query: 339 FTQLLTLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDE 512
F++ L L AN++ +D D+ + AFK FD++ G I + LRH ++ G+K + +EVD+
Sbjct: 89 FSEFLNLMANQLQ-ETDADEELKEAFKVFDKDQNGYISASELRHVMINLGEKLTDEEVDQ 147
Query: 513 AYDQMDIDDKGYIDTTKLIAML 578
+ D+D G ++ + + M+
Sbjct: 148 MIKEADLDGDGQVNYDEFVRMM 169
>At2g27030.3 68415.m03247 calmodulin-2/3/5 (CAM5) (TCH1) identical
to calmodulin GI:474183 from [Arabidopsis thaliana],
SP|P25069 Calmodulin-2/3/5 {Arabidopsis thaliana}
Length = 181
Score = 94.7 bits (225), Expect = 7e-20
Identities = 51/145 (35%), Positives = 81/145 (55%), Gaps = 6/145 (4%)
Frame = +3
Query: 180 QVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQ 347
Q++EFKEAF L D D DG I +L SLG+ +E EL +M+ E +G I+F +
Sbjct: 9 QISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPE 68
Query: 348 LLTLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYD 521
L L A +M +D ++ + AF+ FD++ G I + LRH + G+K + +EVDE
Sbjct: 69 FLNLMARKMK-DTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIK 127
Query: 522 QMDIDDKGYIDTTKLIAMLTASAEG 596
+ D+D G I+ + + ++ A G
Sbjct: 128 EADVDGDGQINYEEFVKVMMAKRRG 152
>At5g21274.1 68418.m02533 calmodulin-6 (CAM6) identical to
calmodulin-6 SP:Q03509 from [Arabidopsis thaliana];
contains Pfam profile: PF00036 EF hand
Length = 149
Score = 93.5 bits (222), Expect = 2e-19
Identities = 51/141 (36%), Positives = 80/141 (56%), Gaps = 6/141 (4%)
Frame = +3
Query: 180 QVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQ 347
Q++EFKEAF L D D DG I +L SLG+ +E EL +M+ E +G I+F +
Sbjct: 9 QISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPE 68
Query: 348 LLTLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYD 521
L L A +M +D ++ + AF+ FD++ G I + LRH + G+K S +EVDE
Sbjct: 69 FLNLMARKMK-DTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLSDEEVDEMIR 127
Query: 522 QMDIDDKGYIDTTKLIAMLTA 584
+ D+D G I+ + + ++ A
Sbjct: 128 EADVDGDGQINYEEFVKVMMA 148
>At3g56800.1 68416.m06317 calmodulin-2/3/5 (CAM3) identical to
calmodulin GI:474183 from [Arabidopsis thaliana]; almost
identical to calmodulin-2/3/5 SP:P25069 [Arabidopsis
thaliana]
Length = 149
Score = 92.7 bits (220), Expect = 3e-19
Identities = 50/141 (35%), Positives = 80/141 (56%), Gaps = 6/141 (4%)
Frame = +3
Query: 180 QVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQ 347
Q++EFKEAF L D D DG I +L SLG+ +E EL +M+ E +G I+F +
Sbjct: 9 QISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPE 68
Query: 348 LLTLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYD 521
L L A +M +D ++ + AF+ FD++ G I + LRH + G+K + +EVDE
Sbjct: 69 FLNLMARKMK-DTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIK 127
Query: 522 QMDIDDKGYIDTTKLIAMLTA 584
+ D+D G I+ + + ++ A
Sbjct: 128 EADVDGDGQINYEEFVKVMMA 148
>At2g41110.1 68415.m05078 calmodulin-2/3/5 (CAM2) (CAL1) almost
identical to Calmodulin-2/3/5 SP:P25069 from
[Arabidopsis thaliana]
Length = 149
Score = 92.7 bits (220), Expect = 3e-19
Identities = 50/141 (35%), Positives = 80/141 (56%), Gaps = 6/141 (4%)
Frame = +3
Query: 180 QVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQ 347
Q++EFKEAF L D D DG I +L SLG+ +E EL +M+ E +G I+F +
Sbjct: 9 QISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPE 68
Query: 348 LLTLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYD 521
L L A +M +D ++ + AF+ FD++ G I + LRH + G+K + +EVDE
Sbjct: 69 FLNLMARKMK-DTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIK 127
Query: 522 QMDIDDKGYIDTTKLIAMLTA 584
+ D+D G I+ + + ++ A
Sbjct: 128 EADVDGDGQINYEEFVKVMMA 148
>At2g27030.1 68415.m03245 calmodulin-2/3/5 (CAM5) (TCH1) identical
to calmodulin GI:474183 from [Arabidopsis thaliana],
SP|P25069 Calmodulin-2/3/5 {Arabidopsis thaliana}
Length = 149
Score = 92.7 bits (220), Expect = 3e-19
Identities = 50/141 (35%), Positives = 80/141 (56%), Gaps = 6/141 (4%)
Frame = +3
Query: 180 QVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQ 347
Q++EFKEAF L D D DG I +L SLG+ +E EL +M+ E +G I+F +
Sbjct: 9 QISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPE 68
Query: 348 LLTLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYD 521
L L A +M +D ++ + AF+ FD++ G I + LRH + G+K + +EVDE
Sbjct: 69 FLNLMARKMK-DTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIK 127
Query: 522 QMDIDDKGYIDTTKLIAMLTA 584
+ D+D G I+ + + ++ A
Sbjct: 128 EADVDGDGQINYEEFVKVMMA 148
>At3g43810.1 68416.m04682 calmodulin-7 (CAM7) almost identical to
calmodulin GI:16227 from [Arabidopsis thaliana],
SP|P59220 Calmodulin-7 {Arabidopsis thaliana}
Length = 149
Score = 92.3 bits (219), Expect = 4e-19
Identities = 50/141 (35%), Positives = 80/141 (56%), Gaps = 6/141 (4%)
Frame = +3
Query: 180 QVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQ 347
Q++EFKEAF L D D DG I +L SLG+ +E EL +M+ E +G I+F +
Sbjct: 9 QISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPE 68
Query: 348 LLTLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYD 521
L L A +M +D ++ + AF+ FD++ G I + LRH + G+K + +EVDE
Sbjct: 69 FLNLMARKMK-DTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIR 127
Query: 522 QMDIDDKGYIDTTKLIAMLTA 584
+ D+D G I+ + + ++ A
Sbjct: 128 EADVDGDGQINYEEFVKVMMA 148
>At5g37780.1 68418.m04549 calmodulin-1/4 (CAM1) identical to
calmodulin 4 [Arabidopsis thaliana] GI:16223, SP|P25854
Calmodulin-1/4 {Arabidopsis thaliana}
Length = 149
Score = 91.5 bits (217), Expect = 6e-19
Identities = 49/144 (34%), Positives = 82/144 (56%), Gaps = 6/144 (4%)
Frame = +3
Query: 171 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 338
+ +Q++EFKEAF L D D DG I +L SLG+ +E EL +M+ E +G I+
Sbjct: 6 TDEQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTID 65
Query: 339 FTQLLTLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDE 512
F + L L A +M +D ++ + AF+ FD++ G I + LRH + G+K + +EV+E
Sbjct: 66 FPEFLNLMAKKMK-DTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVEE 124
Query: 513 AYDQMDIDDKGYIDTTKLIAMLTA 584
+ D+D G I+ + + ++ A
Sbjct: 125 MIREADVDGDGQINYEEFVKIMMA 148
>At1g66410.1 68414.m07542 calmodulin-1/4 (CAM4) identical to
calmodulin [Arabidopsis thaliana] GI:16223; nearly
identical to SP|P25854 Calmodulin-1/4 {Arabidopsis
thaliana}
Length = 149
Score = 91.5 bits (217), Expect = 6e-19
Identities = 49/144 (34%), Positives = 82/144 (56%), Gaps = 6/144 (4%)
Frame = +3
Query: 171 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 338
+ +Q++EFKEAF L D D DG I +L SLG+ +E EL +M+ E +G I+
Sbjct: 6 TDEQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTID 65
Query: 339 FTQLLTLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDE 512
F + L L A +M +D ++ + AF+ FD++ G I + LRH + G+K + +EV+E
Sbjct: 66 FPEFLNLMAKKMK-DTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVEE 124
Query: 513 AYDQMDIDDKGYIDTTKLIAMLTA 584
+ D+D G I+ + + ++ A
Sbjct: 125 MIREADVDGDGQINYEEFVKIMMA 148
>At4g14640.1 68417.m02252 calmodulin-8 (CAM8) identical to
calmodulin 8 GI:5825600 from [Arabidopsis thaliana]
Length = 151
Score = 83.4 bits (197), Expect = 2e-16
Identities = 45/142 (31%), Positives = 80/142 (56%), Gaps = 6/142 (4%)
Frame = +3
Query: 171 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 338
++ Q+ EFKEAF L D D DG I +L SL + +E+EL +++ E ++G I
Sbjct: 7 TKDQITEFKEAFCLFDKDGDGCITVEELATVIRSLDQNPTEQELHDIITEIDSDSNGTIE 66
Query: 339 FTQLLTLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDE 512
F + L L A ++ SD ++ + AFK FD++ G I + L H ++ G+K + +EV++
Sbjct: 67 FAEFLNLMAKKLQ-ESDAEEELKEAFKVFDKDQNGYISASELSHVMINLGEKLTDEEVEQ 125
Query: 513 AYDQMDIDDKGYIDTTKLIAML 578
+ D+D G ++ + + M+
Sbjct: 126 MIKEADLDGDGQVNYDEFVKMM 147
>At3g51920.1 68416.m05695 calmodulin-9 (CAM9) identical to
calmodulin 9 GI:5825602 from [Arabidopsis thaliana];
contains Pfam profile PF00036: EF hand
Length = 151
Score = 72.1 bits (169), Expect = 4e-13
Identities = 43/147 (29%), Positives = 72/147 (48%), Gaps = 6/147 (4%)
Frame = +3
Query: 168 FSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPI 335
F+ +Q+ EF EAF L+D D DG I K L S+G+ ++L +M+ + +G I
Sbjct: 5 FTDEQIQEFYEAFCLIDKDSDGFITKEKLTKVMKSMGKNPKAEQLQQMMSDVDIFGNGGI 64
Query: 336 NFTQLLTLFANRMSGGSDEDDVVINAFKTFDEEGK--IDSERLRHALMTWGDKFSADEVD 509
F L + A S S D+ +I F+ FD +G I L + G K +A+E +
Sbjct: 65 TFDDFLYIMAQNTSQESASDE-LIEVFRVFDRDGDGLISQLELGEGMKDMGMKITAEEAE 123
Query: 510 EAYDQMDIDDKGYIDTTKLIAMLTASA 590
+ D+D G++ + M+ A++
Sbjct: 124 HMVREADLDGDGFLSFHEFSKMMIAAS 150
>At1g62820.1 68414.m07092 calmodulin, putative similar to calmodulin
SP:P04465 from [Trypanosoma brucei gambiense]; contains
INTERPRO:IPR002048 calcium-binding EF-hand domain
Length = 148
Score = 68.5 bits (160), Expect = 5e-12
Identities = 47/142 (33%), Positives = 67/142 (47%), Gaps = 4/142 (2%)
Frame = +3
Query: 171 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV--GEASGPINFT 344
S QV+ KEAF L D D DG I ++L SLG +E +L ++ S P +F
Sbjct: 7 SNDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTESQLKSIITTENLSSPFDFN 66
Query: 345 QLLTLFANRMSGGSDEDDVVINAFKTFDEEGK--IDSERLRHALMTWGDKFSADEVDEAY 518
+ L L A + D + +AFK D+EG + LRH L + G+K E DE
Sbjct: 67 RFLDLMAKHLK-TEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLQPSEFDEWI 125
Query: 519 DQMDIDDKGYIDTTKLIAMLTA 584
++D+ G I IA + A
Sbjct: 126 KEVDVGSDGKIRYEDFIARMVA 147
>At1g12310.1 68414.m01423 calmodulin, putative similar to calmodulin
SP:P04465 from [Trypanosoma brucei gambiense]
Length = 148
Score = 68.1 bits (159), Expect = 7e-12
Identities = 46/142 (32%), Positives = 68/142 (47%), Gaps = 4/142 (2%)
Frame = +3
Query: 171 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVG--EASGPINFT 344
S QV+ KEAF L D D DG I ++L SLG ++ +L ++ S P +F
Sbjct: 7 SDDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTQAQLKSIIASENLSSPFDFN 66
Query: 345 QLLTLFANRMSGGSDEDDVVINAFKTFDEEGK--IDSERLRHALMTWGDKFSADEVDEAY 518
+ L L A + D + +AFK D+EG + LRH L + G+K +E DE
Sbjct: 67 RFLDLMAKHLK-TEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLEPNEFDEWI 125
Query: 519 DQMDIDDKGYIDTTKLIAMLTA 584
++D+ G I IA + A
Sbjct: 126 KEVDVGSDGKIRYEDFIARMVA 147
>At2g27030.2 68415.m03246 calmodulin-2/3/5 (CAM5) (TCH1) identical
to calmodulin GI:474183 from [Arabidopsis thaliana],
SP|P25069 Calmodulin-2/3/5 {Arabidopsis thaliana}
Length = 113
Score = 65.7 bits (153), Expect = 4e-11
Identities = 36/111 (32%), Positives = 63/111 (56%), Gaps = 6/111 (5%)
Frame = +3
Query: 270 SLGRLASEKELDEMVGEA----SGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFDEE- 434
SLG+ +E EL +M+ E +G I+F + L L A +M +D ++ + AF+ FD++
Sbjct: 3 SLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMARKMKD-TDSEEELKEAFRVFDKDQ 61
Query: 435 -GKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTA 584
G I + LRH + G+K + +EVDE + D+D G I+ + + ++ A
Sbjct: 62 NGFISAAELRHVMTNLGEKLTDEEVDEMIKEADVDGDGQINYEEFVKVMMA 112
Score = 45.2 bits (102), Expect = 5e-05
Identities = 22/59 (37%), Positives = 36/59 (61%), Gaps = 4/59 (6%)
Frame = +3
Query: 189 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLL 353
E KEAF++ D D++G I +LR +LG +++E+DEM+ EA G IN+ + +
Sbjct: 49 ELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIKEADVDGDGQINYEEFV 107
>At3g50360.1 68416.m05507 caltractin / centrin identical to
caltractin; centrin GI:3688162 from [Arabidopsis
thaliana]
Length = 169
Score = 64.9 bits (151), Expect = 6e-11
Identities = 38/143 (26%), Positives = 73/143 (51%), Gaps = 6/143 (4%)
Frame = +3
Query: 189 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVG----EASGPINFTQLLT 356
E KEAF+L D D G I +L +LG +E+++++M+ + SG I+F + +
Sbjct: 27 EIKEAFELFDTDGSGTIDAKELNVAMRALGFEMTEEQINKMIADVDKDGSGAIDFDEFVH 86
Query: 357 LFANRMSGGSDEDDVVINAFK--TFDEEGKIDSERLRHALMTWGDKFSADEVDEAYDQMD 530
+ ++ G D + + AF+ D+ GKI + ++ G+ F+ E+ E ++ D
Sbjct: 87 MMTAKI-GERDTKEELTKAFQIIDLDKNGKISPDDIKRMAKDLGENFTDAEIREMVEEAD 145
Query: 531 IDDKGYIDTTKLIAMLTASAEGG 599
D G ++ + + M+ +A GG
Sbjct: 146 RDRDGEVNMDEFMRMMRRTAYGG 168
Score = 41.1 bits (92), Expect = 9e-04
Identities = 22/70 (31%), Positives = 37/70 (52%), Gaps = 4/70 (5%)
Frame = +3
Query: 189 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLLT 356
E +AFQ++D DK+G I +D++ LG ++ E+ EMV EA G +N + +
Sbjct: 100 ELTKAFQIIDLDKNGKISPDDIKRMAKDLGENFTDAEIREMVEEADRDRDGEVNMDEFMR 159
Query: 357 LFANRMSGGS 386
+ GG+
Sbjct: 160 MMRRTAYGGN 169
>At1g76040.2 68414.m08829 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase GB:AAC25423 GI:3283996 [Nicotiana tabacum]
Length = 534
Score = 64.5 bits (150), Expect = 8e-11
Identities = 45/160 (28%), Positives = 84/160 (52%), Gaps = 7/160 (4%)
Frame = +3
Query: 120 RGSRKAKRTGSNVFSM-FSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEK 296
R K K+ V + S++++ K+ F+ MD D+ G I ++LR LG +E
Sbjct: 365 RAMNKLKKLALKVIAENLSEEEIKGLKQTFKNMDTDESGTITFDELRNGLHRLGSKLTES 424
Query: 297 ELDEMVGEA----SGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERL 458
E+ +++ A SG I++ + +T +R +E+ +I AFK FD++ G I + L
Sbjct: 425 EIKQLMEAADVDKSGTIDYIEFVTATMHRHRLEKEEN--LIEAFKYFDKDRSGFITRDEL 482
Query: 459 RHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAML 578
+H++ +G A +DE + +D D+ G I+ + +AM+
Sbjct: 483 KHSMTEYGMGDDAT-IDEVINDVDTDNDGRINYEEFVAMM 521
>At1g76040.1 68414.m08830 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase GB:AAC25423 GI:3283996 [Nicotiana tabacum]
Length = 323
Score = 64.5 bits (150), Expect = 8e-11
Identities = 45/160 (28%), Positives = 84/160 (52%), Gaps = 7/160 (4%)
Frame = +3
Query: 120 RGSRKAKRTGSNVFSM-FSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEK 296
R K K+ V + S++++ K+ F+ MD D+ G I ++LR LG +E
Sbjct: 154 RAMNKLKKLALKVIAENLSEEEIKGLKQTFKNMDTDESGTITFDELRNGLHRLGSKLTES 213
Query: 297 ELDEMVGEA----SGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERL 458
E+ +++ A SG I++ + +T +R +E+ +I AFK FD++ G I + L
Sbjct: 214 EIKQLMEAADVDKSGTIDYIEFVTATMHRHRLEKEEN--LIEAFKYFDKDRSGFITRDEL 271
Query: 459 RHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAML 578
+H++ +G A +DE + +D D+ G I+ + +AM+
Sbjct: 272 KHSMTEYGMGDDAT-IDEVINDVDTDNDGRINYEEFVAMM 310
>At3g03000.1 68416.m00295 calmodulin, putative similar to calmodulin
SP:P04352 from [Chlamydomonas reinhardtii]; contains
Pfam profile: PF00036 EF hand (4 copies)
Length = 165
Score = 63.3 bits (147), Expect = 2e-10
Identities = 39/144 (27%), Positives = 74/144 (51%), Gaps = 6/144 (4%)
Frame = +3
Query: 177 KQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFT 344
+Q+AE +E F+ D +KDG + + +L + SLG S+ +LD ++ +A +G + F+
Sbjct: 16 EQLAELREIFRSFDQNKDGSLTELELGSLLRSLGLKPSQDQLDTLIQKADRNNNGLVEFS 75
Query: 345 QLLTLFANRMSGGSDEDDVVINAFKTFDEEGK--IDSERLRHALMTWGDKFSADEVDEAY 518
+ + L + DD + F+ FD +G I + L H++ G +A+E+
Sbjct: 76 EFVALVEPDLVKCPYTDDQLKAIFRMFDRDGNGYITAAELAHSMAKLGHALTAEELTGMI 135
Query: 519 DQMDIDDKGYIDTTKLIAMLTASA 590
+ D D G ID + + +T++A
Sbjct: 136 KEADRDGDGCIDFQEFVQAITSAA 159
>At2g41090.1 68415.m05075 calmodulin-like calcium-binding protein,
22 kDa (CaBP-22) identical to SP|P30187 22 kDa
calmodulin-like calcium-binding protein (CABP-22)
[Arabidopsis thaliana]
Length = 191
Score = 60.5 bits (140), Expect = 1e-09
Identities = 41/149 (27%), Positives = 71/149 (47%), Gaps = 6/149 (4%)
Frame = +3
Query: 168 FSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPI 335
F+++Q++EF+E F + D + DG I + A SLG ++ EL E + ++ G I
Sbjct: 5 FTRQQISEFREQFSVYDKNGDGHITTEEFGAVMRSLGLNLTQAELQEEINDSDLDGDGTI 64
Query: 336 NFTQLLTLFANRMSGGSDEDDVVINAFKTF--DEEGKIDSERLRHALMTWGDKFSADEVD 509
NFT+ L A D + F+ F D+ G I + +R+ K + +E+D
Sbjct: 65 NFTEFLCAMAKDTYSEKD----LKKDFRLFDIDKNGFISAAEMRYVRTILRWKQTDEEID 120
Query: 510 EAYDQMDIDDKGYIDTTKLIAMLTASAEG 596
E D+D G I+ + ++ A +G
Sbjct: 121 EIIKAADVDGDGQINYREFARLMMAKNQG 149
>At5g23580.1 68418.m02767 calcium-dependent protein kinase 9 (CDPK9)
identical to calcium-dependent protein kinase
[Arabidopsis thaliana] gi|836938|gb|AAA67653; contains
protein kinase domain, Pfam:PF00069; contains EF hand
domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 490
Score = 60.1 bits (139), Expect = 2e-09
Identities = 49/177 (27%), Positives = 87/177 (49%), Gaps = 9/177 (5%)
Frame = +3
Query: 132 KAKRTGSNVFS-MFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDE 308
K K+ V + S++++ KE F+++D DK G I +L+ + +G E E+ E
Sbjct: 307 KLKKMALRVIAERLSEEEIGGLKELFKMIDTDKSGTITFEELKDSMRRVGSELMESEIQE 366
Query: 309 MVGEA----SGPINFTQLL--TLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERLRH 464
++ A SG I++ + L T+ N++ + ++ ++ AF FD++ G I E L+
Sbjct: 367 LLRAADVDESGTIDYGEFLAATIHLNKL----EREENLVAAFSFFDKDASGYITIEELQQ 422
Query: 465 ALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTASAEGGGRW*SRVMIPSL 635
A +G + +DE +D D+ G ID + +AM+ GG R M SL
Sbjct: 423 AWKEFG--INDSNLDEMIKDIDQDNDGQIDYGEFVAMMRKGNGTGGGIGRRTMRNSL 477
>At4g12860.1 68417.m02014 calcium-binding protein, putative similar
to calcium-binding protein GI:6580549 from [Lotus
japonicus]
Length = 152
Score = 59.7 bits (138), Expect = 2e-09
Identities = 40/146 (27%), Positives = 74/146 (50%), Gaps = 8/146 (5%)
Frame = +3
Query: 189 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQLLT 356
E FQ+ D + DG I KN+L+ F S+G + E E++EM+ + G ++ + +
Sbjct: 5 ELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGS 64
Query: 357 LFANRMSGGSDEDDVVINAFKTFDE--EGKIDSERLRHALMTWGDK--FSADEVDEAYDQ 524
L+ + +E+D + AF+ FD+ +G I E LR L + G K + ++ + +
Sbjct: 65 LYQEMVEEKEEEED-MREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISK 123
Query: 525 MDIDDKGYIDTTKLIAMLTASAEGGG 602
+D+D G ++ + M+ GGG
Sbjct: 124 VDVDGDGMVNFKEFKQMM----RGGG 145
Score = 31.5 bits (68), Expect = 0.70
Identities = 25/94 (26%), Positives = 42/94 (44%), Gaps = 7/94 (7%)
Frame = +3
Query: 123 GSRKAKRTGSNVFSMFSQKQVAE-FKEAFQLMDHDKDGIIGKNDLRATFDSLGRLAS--- 290
G+ GS M +K+ E +EAF++ D + DG I +LR+ S+G
Sbjct: 55 GAMDIDEFGSLYQEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTL 114
Query: 291 ---EKELDEMVGEASGPINFTQLLTLFANRMSGG 383
+K + ++ + G +NF + F M GG
Sbjct: 115 EDCKKMISKVDVDGDGMVNFKE----FKQMMRGG 144
>At2g15680.1 68415.m01795 calmodulin-related protein, putative
similar to calmodulin-related protein 2, touch-induced
SP:P25070 from [Arabidopsis thaliana]
Length = 187
Score = 58.8 bits (136), Expect = 4e-09
Identities = 39/146 (26%), Positives = 74/146 (50%), Gaps = 6/146 (4%)
Frame = +3
Query: 168 FSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVG----EASGPI 335
FSQ V E + F D DKDG I + + + +LG+ + +++ ++ + G I
Sbjct: 43 FSQPSVNEMRRVFSRFDLDKDGKISQTEYKVVLRALGQERAIEDVPKIFKAVDLDGDGFI 102
Query: 336 NFTQLLTLFANRMSGGSDEDDVVINAFKTFD--EEGKIDSERLRHALMTWGDKFSADEVD 509
+F + + A + SGG D + N+F TFD +GKI +E + L G++ S ++ +
Sbjct: 103 DFREFID--AYKRSGGIRSSD-IRNSFWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCN 159
Query: 510 EAYDQMDIDDKGYIDTTKLIAMLTAS 587
+D D G ++ + I M++++
Sbjct: 160 RMVRAVDADGDGLVNMEEFIKMMSSN 185
>At2g41100.1 68415.m05076 touch-responsive protein /
calmodulin-related protein 3, touch-induced (TCH3)
identical to calmodulin-related protein 3, touch-induced
SP:P25071 from [Arabidopsis thaliana]
Length = 324
Score = 58.4 bits (135), Expect = 5e-09
Identities = 47/180 (26%), Positives = 86/180 (47%), Gaps = 25/180 (13%)
Frame = +3
Query: 117 SRGSRKAKR-TGSNVFSMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASE 293
++G +A R T + + Q+ E++E+F+L D + DG I K +LR SLG+ ++
Sbjct: 76 NQGHDQAPRHTKKTMADKLTDDQITEYRESFRLFDKNGDGSITKKELRTVMFSLGKNRTK 135
Query: 294 KELDEMVGE----ASGPINFTQLLTLFANRMSGGSDE---------------DDVVI--- 407
+L +M+ E G I+F + L L A G D+ DD ++
Sbjct: 136 ADLQDMMNEVDLDGDGTIDFPEFLYLMAKNQ--GHDQAPRHTKKTMVDYQLTDDQILEFR 193
Query: 408 NAFKTFDE--EGKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLT 581
AF+ FD+ +G I LR + + G+ + E+ + ++ D D G I ++ + ++T
Sbjct: 194 EAFRVFDKNGDGYITVNELRTTMRSLGETQTKAELQDMINEADADGDGTISFSEFVCVMT 253
Score = 53.6 bits (123), Expect = 2e-07
Identities = 26/69 (37%), Positives = 42/69 (60%), Gaps = 4/69 (5%)
Frame = +3
Query: 180 QVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQ 347
Q+ EF+EAF++ D + DG I N+LR T SLG ++ EL +M+ EA G I+F++
Sbjct: 188 QILEFREAFRVFDKNGDGYITVNELRTTMRSLGETQTKAELQDMINEADADGDGTISFSE 247
Query: 348 LLTLFANRM 374
+ + +M
Sbjct: 248 FVCVMTGKM 256
Score = 51.6 bits (118), Expect = 6e-07
Identities = 36/154 (23%), Positives = 75/154 (48%), Gaps = 21/154 (13%)
Frame = +3
Query: 180 QVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQ 347
Q+ E++E+F+L D + DG I K +L S+G ++ +L +++ EA G I+F +
Sbjct: 9 QITEYRESFRLFDKNGDGSITKKELGTMMRSIGEKPTKADLQDLMNEADLDGDGTIDFPE 68
Query: 348 LLTLFANR-------------MSGGSDEDDVV--INAFKTFDE--EGKIDSERLRHALMT 476
L + A M+ +D + +F+ FD+ +G I + LR + +
Sbjct: 69 FLCVMAKNQGHDQAPRHTKKTMADKLTDDQITEYRESFRLFDKNGDGSITKKELRTVMFS 128
Query: 477 WGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAML 578
G + ++ + +++D+D G ID + + ++
Sbjct: 129 LGKNRTKADLQDMMNEVDLDGDGTIDFPEFLYLM 162
>At1g24620.1 68414.m03097 polcalcin, putative / calcium-binding
pollen allergen, putative similar to polcalcin Jun o 2
(calcium-binding pollen allergen Jun o 2) SP:O64943 from
[Juniperus oxycedrus]
Length = 186
Score = 57.6 bits (133), Expect = 9e-09
Identities = 44/143 (30%), Positives = 68/143 (47%), Gaps = 9/143 (6%)
Frame = +3
Query: 180 QVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQ 347
++ E + F+ D + DG I +L A SLG E+EL++ + E G INF +
Sbjct: 34 EIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEE 93
Query: 348 LLTLFANRMSGGSDEDDVVIN---AFKTFDEEGK--IDSERLRHALMTWGDKFSADEVDE 512
+ L G D++DV+ N AF +D +G I +E L L + GD+ S E +
Sbjct: 94 FVELNTK----GMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRK 149
Query: 513 AYDQMDIDDKGYIDTTKLIAMLT 581
+D D G ID + M+T
Sbjct: 150 MIGGVDKDGDGTIDFEEFKIMMT 172
Score = 33.1 bits (72), Expect = 0.23
Identities = 27/82 (32%), Positives = 39/82 (47%), Gaps = 5/82 (6%)
Frame = +3
Query: 174 QKQVAE-FKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVG----EASGPIN 338
Q V E K+AF + D D +G I +L SLG S E +M+G + G I+
Sbjct: 104 QNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDGDGTID 163
Query: 339 FTQLLTLFANRMSGGSDEDDVV 404
F + F M+ GS D+V+
Sbjct: 164 FEE----FKIMMTMGSRRDNVM 181
>At5g12480.1 68418.m01466 calmodulin-domain protein kinase isoform 7
(CPK7) identical to calmodulin-domain protein kinase
CDPK isoform 7 [Arabidopsis thaliana]
gi|1399277|gb|AAB03247
Length = 535
Score = 56.8 bits (131), Expect = 2e-08
Identities = 45/169 (26%), Positives = 82/169 (48%), Gaps = 7/169 (4%)
Frame = +3
Query: 108 RQSSRGSRKAKRTGSNVFSMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLA 287
+Q S ++ KR + S ++ A KEAF++MD +K G I +L+ G+
Sbjct: 337 KQFSVMNKLKKRALRVIAEHLSVEEAAGIKEAFEMMDVNKRGKINLEELKYGLQKAGQQI 396
Query: 288 SEKELDEMVG----EASGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFD--EEGKIDS 449
++ +L ++ + G +N+++ + + + +DE + AF FD + G I+
Sbjct: 397 ADTDLQILMEATDVDGDGTLNYSEFVAVSVHLKKMANDEH--LHKAFNFFDQNQSGYIEI 454
Query: 450 ERLRHALMTWGDKFSADEVDEAYDQ-MDIDDKGYIDTTKLIAMLTASAE 593
+ LR AL D S++EV A Q +D D G I + +AM+ A +
Sbjct: 455 DELREALNDELDNTSSEEVIAAIMQDVDTDKDGRISYEEFVAMMKAGTD 503
>At2g41100.2 68415.m05077 touch-responsive protein /
calmodulin-related protein 3, touch-induced (TCH3)
identical to calmodulin-related protein 3, touch-induced
SP:P25071 from [Arabidopsis thaliana]
Length = 235
Score = 53.6 bits (123), Expect = 2e-07
Identities = 26/69 (37%), Positives = 42/69 (60%), Gaps = 4/69 (5%)
Frame = +3
Query: 180 QVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQ 347
Q+ EF+EAF++ D + DG I N+LR T SLG ++ EL +M+ EA G I+F++
Sbjct: 99 QILEFREAFRVFDKNGDGYITVNELRTTMRSLGETQTKAELQDMINEADADGDGTISFSE 158
Query: 348 LLTLFANRM 374
+ + +M
Sbjct: 159 FVCVMTGKM 167
Score = 52.4 bits (120), Expect = 3e-07
Identities = 40/158 (25%), Positives = 76/158 (48%), Gaps = 24/158 (15%)
Frame = +3
Query: 180 QVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQ 347
Q+ E++E+F+L D + DG I K +L S+G ++ +L +++ EA G I+F +
Sbjct: 9 QITEYRESFRLFDKNGDGSITKKELGTMMRSIGEKPTKADLQDLMNEADLDGDGTIDFPE 68
Query: 348 LLTLFANRMSGGSDE---------------DDVVI---NAFKTFDE--EGKIDSERLRHA 467
L + A G D+ DD ++ AF+ FD+ +G I LR
Sbjct: 69 FLCVMAKNQ--GHDQAPRHTKKTMVDYQLTDDQILEFREAFRVFDKNGDGYITVNELRTT 126
Query: 468 LMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLT 581
+ + G+ + E+ + ++ D D G I ++ + ++T
Sbjct: 127 MRSLGETQTKAELQDMINEADADGDGTISFSEFVCVMT 164
>At1g05990.1 68414.m00627 calcium-binding protein, putative strong
similarity to calcium-binding protein [Lotus japonicus]
GI:18413495; contains INTERPRO:IPR002048 calcium-binding
EF-hand domain
Length = 150
Score = 52.0 bits (119), Expect = 5e-07
Identities = 39/146 (26%), Positives = 70/146 (47%), Gaps = 8/146 (5%)
Frame = +3
Query: 189 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQLLT 356
E K FQ+ D + DG I +L T SLG +KEL +M+ + G ++ +
Sbjct: 5 ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEFGE 64
Query: 357 LFANRMSGGSDEDDVVINAFKTFDE--EGKIDSERLRHALMTWGDK--FSADEVDEAYDQ 524
L+ M +E++ + AF FD+ +G I + L+ L + G K + D+ + +
Sbjct: 65 LYKTIMDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDCKKMIKK 124
Query: 525 MDIDDKGYIDTTKLIAMLTASAEGGG 602
+D+D G ++ + M+ +GGG
Sbjct: 125 VDVDGDGRVNYKEFRQMM----KGGG 146
>At1g18890.1 68414.m02351 calcium-dependent protein kinase 1 (CDPK1)
identical to calcium-dependent protein kinase
[Arabidopsis thaliana] gi|604880|dbj|BAA04829; contains
protein kinase domain, Pfam:PF00069; contains EF hand
domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 545
Score = 51.2 bits (117), Expect = 8e-07
Identities = 40/168 (23%), Positives = 78/168 (46%), Gaps = 6/168 (3%)
Frame = +3
Query: 108 RQSSRGSRKAKRTGSNVFSMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLA 287
+Q S +R K+ + S ++V K F LMD DKDG I +L+A +G
Sbjct: 341 KQFSMMNRFKKKVLRVIAEHLSIQEVEVIKNMFSLMDDDKDGKITYPELKAGLQKVGSQL 400
Query: 288 SEKELDEMVG----EASGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFDEEGK--IDS 449
E E+ ++ + +G +++ + + + + + D++ AF FD++G I+
Sbjct: 401 GEPEIKMLMEVADVDGNGFLDYGEFVAVIIHLQK--IENDELFKLAFMFFDKDGSTYIEL 458
Query: 450 ERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTASAE 593
+ LR AL + A + + ++D D G I+ + + M+ A +
Sbjct: 459 DELREALADELGEPDASVLSDIMREVDTDKDGRINYDEFVTMMKAGTD 506
>At5g37770.1 68418.m04547 touch-responsive protein /
calmodulin-related protein 2, touch-induced (TCH2)
identical to calmodulin-related protein 2,touch-induced
SP:P25070 from [Arabidopsis thaliana]
Length = 161
Score = 50.4 bits (115), Expect = 1e-06
Identities = 38/147 (25%), Positives = 74/147 (50%), Gaps = 9/147 (6%)
Frame = +3
Query: 189 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQLLT 356
+ K+ FQ D + DG I ++L+ +L AS +E M+ + +G I+ + +
Sbjct: 17 DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76
Query: 357 LFANRMSGG-SDEDDV--VINAFKTFDEEG--KIDSERLRHALMTWGDKFSADEVDEAYD 521
LF + GG ++ +DV + AF+ +D +G +I ++ L + G+K S + +
Sbjct: 77 LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136
Query: 522 QMDIDDKGYIDTTKLIAMLTASAEGGG 602
++DID G ++ + M+ + GGG
Sbjct: 137 KVDIDGDGCVNFDEFKKMM---SNGGG 160
Score = 39.5 bits (88), Expect = 0.003
Identities = 20/71 (28%), Positives = 33/71 (46%), Gaps = 2/71 (2%)
Frame = +3
Query: 396 DVVINAFKTFDE--EGKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLI 569
D + F+ FD+ +GKI + L+ + S +E Q D+D G+ID + +
Sbjct: 16 DDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFV 75
Query: 570 AMLTASAEGGG 602
A+ GGG
Sbjct: 76 ALFQIGIGGGG 86
Score = 38.3 bits (85), Expect = 0.006
Identities = 19/70 (27%), Positives = 37/70 (52%), Gaps = 4/70 (5%)
Frame = +3
Query: 171 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVG----EASGPIN 338
++ V++ KEAF+L D D +G I +L + +LG S ++ +M+ + G +N
Sbjct: 88 NRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMISKVDIDGDGCVN 147
Query: 339 FTQLLTLFAN 368
F + + +N
Sbjct: 148 FDEFKKMMSN 157
>At3g51850.1 68416.m05686 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase [Arabidopsis thaliana] gi|836942|gb|AAA67655;
contains protein kinase domain, Pfam:PF00069; contains
EF hand domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 528
Score = 50.0 bits (114), Expect = 2e-06
Identities = 39/165 (23%), Positives = 72/165 (43%), Gaps = 6/165 (3%)
Frame = +3
Query: 117 SRGSRKAKRTGSNVFSMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEK 296
+R RKA R + S ++V + K F MD D DGI+ +L+A +E
Sbjct: 338 NRFKRKALRV---IAEFLSTEEVEDIKVMFNKMDTDNDGIVSIEELKAGLRDFSTQLAES 394
Query: 297 ELDEMV----GEASGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFDEEGK--IDSERL 458
E+ ++ + G +++ + + + + +DE + AF FD++G I + L
Sbjct: 395 EVQMLIEAVDTKGKGTLDYGEFVAVSLHLQKVANDEH--LRKAFSYFDKDGNGYILPQEL 452
Query: 459 RHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTASAE 593
AL G D ++ + ++D D G I + AM+ +
Sbjct: 453 CDALKEDGGDDCVDVANDIFQEVDTDKDGRISYEEFAAMMKTGTD 497
>At5g19450.2 68418.m02318 calcium-dependent protein kinase 19
(CDPK19) identical to calcium-dependent protein kinase
[Arabidopsis thaliana] gi|836942|gb|AAA67655
Length = 533
Score = 49.6 bits (113), Expect = 2e-06
Identities = 45/169 (26%), Positives = 75/169 (44%), Gaps = 7/169 (4%)
Frame = +3
Query: 108 RQSSRGSRKAKRTGSNVFSMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLG-RL 284
+Q S ++ KR + S ++VA KEAF++MD K G I +L+ LG +
Sbjct: 335 KQFSVMNKLKKRALRVIAEHLSVEEVAGIKEAFEMMDSKKTGKINLEELKFGLHKLGQQQ 394
Query: 285 ASEKELDEMVGEA----SGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFDEEGK--ID 446
+ +L ++ A G +N+ + + + + +DE + AF FD+ I+
Sbjct: 395 IPDTDLQILMEAADVDGDGTLNYGEFVAVSVHLKKMANDEH--LHKAFSFFDQNQSDYIE 452
Query: 447 SERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTASAE 593
E LR AL D S + V +D D G I + AM+ A +
Sbjct: 453 IEELREALNDEVDTNSEEVVAAIMQDVDTDKDGRISYEEFAAMMKAGTD 501
>At5g19450.1 68418.m02317 calcium-dependent protein kinase 19
(CDPK19) identical to calcium-dependent protein kinase
[Arabidopsis thaliana] gi|836942|gb|AAA67655
Length = 533
Score = 49.6 bits (113), Expect = 2e-06
Identities = 45/169 (26%), Positives = 75/169 (44%), Gaps = 7/169 (4%)
Frame = +3
Query: 108 RQSSRGSRKAKRTGSNVFSMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLG-RL 284
+Q S ++ KR + S ++VA KEAF++MD K G I +L+ LG +
Sbjct: 335 KQFSVMNKLKKRALRVIAEHLSVEEVAGIKEAFEMMDSKKTGKINLEELKFGLHKLGQQQ 394
Query: 285 ASEKELDEMVGEA----SGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFDEEGK--ID 446
+ +L ++ A G +N+ + + + + +DE + AF FD+ I+
Sbjct: 395 IPDTDLQILMEAADVDGDGTLNYGEFVAVSVHLKKMANDEH--LHKAFSFFDQNQSDYIE 452
Query: 447 SERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTASAE 593
E LR AL D S + V +D D G I + AM+ A +
Sbjct: 453 IEELREALNDEVDTNSEEVVAAIMQDVDTDKDGRISYEEFAAMMKAGTD 501
>At1g74740.1 68414.m08660 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase [Arabidopsis thaliana] gi|604880|dbj|BAA04829;
contains protein kinase domain, Pfam:PF00069; contains
EF hand domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 541
Score = 49.6 bits (113), Expect = 2e-06
Identities = 41/168 (24%), Positives = 76/168 (45%), Gaps = 6/168 (3%)
Frame = +3
Query: 108 RQSSRGSRKAKRTGSNVFSMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLA 287
+Q S +R K+ + S ++V + F LMD D DG I +LRA +G
Sbjct: 337 KQFSMMNRLKKKALRVIAEHLSIQEVEVIRNMFTLMDDDNDGKISYLELRAGLRKVGSQL 396
Query: 288 SEKELDEMVGEA----SGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFDEEGK--IDS 449
E E+ ++ A +G +++ + + + + + D+ AF FD++G I+S
Sbjct: 397 GEPEIKLLMEVADVNGNGCLDYGEFVAVIIHLQK--MENDEHFRQAFMFFDKDGSGYIES 454
Query: 450 ERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTASAE 593
E LR AL + + + ++D D G I+ + + M+ A +
Sbjct: 455 EELREALTDELGEPDNSVIIDIMREVDTDKDGKINYDEFVVMMKAGTD 502
>At4g04695.1 68417.m00689 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase [Lycopersicon esculentum]
gi|19171502|emb|CAC87494; contains protein kinase
domain, Pfam:PF00069; contains EF hand domain
(calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 484
Score = 49.2 bits (112), Expect = 3e-06
Identities = 43/166 (25%), Positives = 77/166 (46%), Gaps = 9/166 (5%)
Frame = +3
Query: 120 RGSRKAKRTGSNVFSM-FSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEK 296
R K K+ V + S++++ K F +D DK G I +L+ LG S+
Sbjct: 312 RDMNKLKKVALKVIAANLSEEEIKGLKTLFTNIDTDKSGTITLEELKTGLTRLGSNLSKT 371
Query: 297 ELDEMVGEA----SGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFDE--EGKIDSERL 458
E+++++ A +G I+ + ++ +R D DD V AF+ FD+ +G I E L
Sbjct: 372 EVEQLMEAADVDGNGTIDIDEFISATMHRYR--LDRDDHVYQAFQHFDKDNDGHITKEEL 429
Query: 459 RHALMTWGDKFSADEVD--EAYDQMDIDDKGYIDTTKLIAMLTASA 590
A+ G DEV + ++D D+ G I+ + M+ + +
Sbjct: 430 EMAMKEHG---VGDEVSIKQIITEVDTDNDGKINFEEFRTMMRSGS 472
Score = 29.1 bits (62), Expect = 3.7
Identities = 18/63 (28%), Positives = 30/63 (47%), Gaps = 3/63 (4%)
Frame = +3
Query: 198 EAFQLMDHDKDGIIGKNDLRATFDSLG---RLASEKELDEMVGEASGPINFTQLLTLFAN 368
+AFQ D D DG I K +L G ++ ++ + E+ + G INF + T+ +
Sbjct: 411 QAFQHFDKDNDGHITKEELEMAMKEHGVGDEVSIKQIITEVDTDNDGKINFEEFRTMMRS 470
Query: 369 RMS 377
S
Sbjct: 471 GSS 473
>At3g50770.1 68416.m05560 calmodulin-related protein, putative
similar to regulator of gene silencing
calmodulin-related protein GI:12963415 from [Nicotiana
tabacum]
Length = 205
Score = 49.2 bits (112), Expect = 3e-06
Identities = 41/146 (28%), Positives = 61/146 (41%), Gaps = 10/146 (6%)
Frame = +3
Query: 174 QKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINF 341
Q E ++ F D D DG I +LR F S+G S + E + E A G + F
Sbjct: 59 QASKEELRQVFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGF 118
Query: 342 TQLLTLFANRM---SGGSDEDDVVINAFKTFDEE---GKIDSERLRHALMTWGDKFSADE 503
+ L R G D D + AF+ F+ E G I + L+ L+ G+ + E
Sbjct: 119 EDFVGLMTRRDLYGDGEVDGDGELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGE 178
Query: 504 VDEAYDQMDIDDKGYIDTTKLIAMLT 581
+ DID G +D + M+T
Sbjct: 179 CEAMIKFYDIDGNGILDFHEFRQMMT 204
>At2g41410.1 68415.m05110 calmodulin, putative identical to
SP|P30188 Calmodulin-like protein {Arabidopsis thaliana}
Length = 216
Score = 48.4 bits (110), Expect = 6e-06
Identities = 33/107 (30%), Positives = 48/107 (44%), Gaps = 7/107 (6%)
Frame = +3
Query: 189 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKE-----LDEMVGEASGPINFTQLL 353
E +AF+L+D D DG++ + DL A L +E L E+ G G I+ L
Sbjct: 70 ELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGGCISLEDLA 129
Query: 354 TLFANRMSGGSDEDDVVINAFKTF--DEEGKIDSERLRHALMTWGDK 488
+ A GS E + + F+ F D GKI +E L GD+
Sbjct: 130 SRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDE 176
>At1g66400.1 68414.m07541 calmodulin-related protein, putative
similar to calmodulin-related protein 2, touch-induced
from SP:P25070 [Arabidopsis thaliana]; contains Pfam
profile: PF00036 EF hand (4 copies)
Length = 157
Score = 46.8 bits (106), Expect = 2e-05
Identities = 38/144 (26%), Positives = 68/144 (47%), Gaps = 10/144 (6%)
Frame = +3
Query: 189 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQLLT 356
+ K+ FQ D + DG I ++L+ +L AS++E M+ E +G I+ + +
Sbjct: 15 DIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVA 74
Query: 357 LF--ANRMSGGSDEDDVVINAFKTFD--EEGKIDSERLRHALMTWGDKFSADEVDEAYDQ 524
LF +++ S S D+ AF +D G+I + L + G+K S + ++
Sbjct: 75 LFQISDQSSNNSAIRDLK-EAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINK 133
Query: 525 MDIDDKGYID--TTKLIAMLTASA 590
+D D G +D K + M+ SA
Sbjct: 134 VDSDGDGCVDFEEFKKMMMINGSA 157
Score = 35.5 bits (78), Expect = 0.043
Identities = 15/46 (32%), Positives = 26/46 (56%)
Frame = +3
Query: 183 VAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE 320
+ + KEAF L D D++G I N+L + +LG S ++ M+ +
Sbjct: 88 IRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINK 133
Score = 34.7 bits (76), Expect = 0.075
Identities = 20/71 (28%), Positives = 34/71 (47%), Gaps = 2/71 (2%)
Frame = +3
Query: 381 GSDEDDVVINAFKTFDE--EGKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYID 554
GS ED + F+ FD+ +GKI + L+ + S +E + D+D G+ID
Sbjct: 11 GSMED--IKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFID 68
Query: 555 TTKLIAMLTAS 587
+ +A+ S
Sbjct: 69 LDEFVALFQIS 79
>At3g10190.1 68416.m01220 calmodulin, putative similar to calmodulin
NtCaM13 [Nicotiana tabacum] GI:14625425, calmodulin
GB:AAA34015 [Glycine max]; contains INTERPRO:IPR002048
calcium-binding EF-hand domain
Length = 209
Score = 45.6 bits (103), Expect = 4e-05
Identities = 37/136 (27%), Positives = 65/136 (47%), Gaps = 6/136 (4%)
Frame = +3
Query: 189 EFKEAFQLMDHDKDGIIGKNDLRATFDSLG-RLASEKELDEMVGEASGPINFTQLLTLFA 365
E +AF+L+D D DG + ++DL + LG +E+E++ M+ E + T L A
Sbjct: 70 EILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEVDCDGDGTIRLEELA 129
Query: 366 NR-MSGGSDEDDVVI-NAFKTF--DEEGKIDSERLRHALMTWGD-KFSADEVDEAYDQMD 530
+R +S D + F+ F D +G I ++ L T GD + + D+ +D
Sbjct: 130 SRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDERCTLDDCKRMIADVD 189
Query: 531 IDDKGYIDTTKLIAML 578
D G++ T+ M+
Sbjct: 190 EDGDGFVCFTEFSRMM 205
Score = 37.9 bits (84), Expect = 0.008
Identities = 16/52 (30%), Positives = 28/52 (53%)
Frame = +3
Query: 123 GSRKAKRTGSNVFSMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLG 278
G+ + + S V S+ + E KE F+ D D+DG+I ++L F ++G
Sbjct: 121 GTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIG 172
>At1g18210.2 68414.m02267 calcium-binding protein, putative similar
to SP|Q9M7R0 Calcium-binding allergen Ole e 8
(PCA18/PCA23) {Olea europaea}; contains
INTERPRO:IPR002048 calcium-binding EF-hand domain
Length = 170
Score = 41.9 bits (94), Expect = 5e-04
Identities = 20/63 (31%), Positives = 33/63 (52%), Gaps = 4/63 (6%)
Frame = +3
Query: 171 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVG----EASGPIN 338
S AE ++AF L D DK+G+I ++L + LG S ++ M+G + G +N
Sbjct: 86 SSSSAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDADGDGNVN 145
Query: 339 FTQ 347
F +
Sbjct: 146 FEE 148
Score = 28.3 bits (60), Expect = 6.5
Identities = 15/61 (24%), Positives = 28/61 (45%), Gaps = 2/61 (3%)
Frame = +3
Query: 414 FKTFDE--EGKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTAS 587
F FD +GKI L G ++ E++ +++D D GYI+ + + +S
Sbjct: 28 FDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFSTLCRSS 87
Query: 588 A 590
+
Sbjct: 88 S 88
>At1g18210.1 68414.m02266 calcium-binding protein, putative similar
to SP|Q9M7R0 Calcium-binding allergen Ole e 8
(PCA18/PCA23) {Olea europaea}; contains
INTERPRO:IPR002048 calcium-binding EF-hand domain
Length = 170
Score = 41.9 bits (94), Expect = 5e-04
Identities = 20/63 (31%), Positives = 33/63 (52%), Gaps = 4/63 (6%)
Frame = +3
Query: 171 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVG----EASGPIN 338
S AE ++AF L D DK+G+I ++L + LG S ++ M+G + G +N
Sbjct: 86 SSSSAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDADGDGNVN 145
Query: 339 FTQ 347
F +
Sbjct: 146 FEE 148
Score = 28.3 bits (60), Expect = 6.5
Identities = 15/61 (24%), Positives = 28/61 (45%), Gaps = 2/61 (3%)
Frame = +3
Query: 414 FKTFDE--EGKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTAS 587
F FD +GKI L G ++ E++ +++D D GYI+ + + +S
Sbjct: 28 FDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFSTLCRSS 87
Query: 588 A 590
+
Sbjct: 88 S 88
>At5g49480.1 68418.m06123 sodium-inducible calcium-binding protein
(ACP1) / sodium-responsive calcium-binding protein
(ACP1) identical to NaCl-inducible Ca2+-binding protein
GI:2352828 from [Arabidopsis thaliana]
Length = 160
Score = 40.3 bits (90), Expect = 0.002
Identities = 21/60 (35%), Positives = 33/60 (55%), Gaps = 4/60 (6%)
Frame = +3
Query: 195 KEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPIN----FTQLLTLF 362
K+ F++MD D DG + DL++ DS G ++ E+ M+ A G +N F LL +F
Sbjct: 99 KDVFKVMDKDGDGRLSYGDLKSYMDSAGLAVTDDEIKSMIRLAGGDLNDGVSFDGLLKIF 158
Score = 37.1 bits (82), Expect = 0.014
Identities = 26/91 (28%), Positives = 44/91 (48%), Gaps = 9/91 (9%)
Frame = +3
Query: 192 FKEAFQLMDHDKDGIIGKNDLRATFDSL--GRLASEKELDEMVGEA----SGPINFTQ-- 347
F+ AF+++D D DG I +DLRA + + G E + M+ A G + F +
Sbjct: 19 FRPAFEIIDTDHDGKISSDDLRAFYAGIPSGENNDETMIGTMISVADANKDGFVEFDEFE 78
Query: 348 -LLTLFANRMSGGSDEDDVVINAFKTFDEEG 437
+L SG +D ++ + FK D++G
Sbjct: 79 KVLETTPFSRSGNGGDDGLMKDVFKVMDKDG 109
>At5g17470.1 68418.m02050 calmodulin-related protein, putative
similar to calmodulin-related protein 2, touch-induced
SP:P25070 from [Arabidopsis thaliana]
Length = 146
Score = 39.9 bits (89), Expect = 0.002
Identities = 29/137 (21%), Positives = 59/137 (43%), Gaps = 7/137 (5%)
Frame = +3
Query: 198 EAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQLLTLFA 365
E F+ +D +KDG I ++ + + +E+D M E I+ + +
Sbjct: 5 EIFERVDKNKDGKISWDEFAEAIRAFSPSITSEEIDNMFREIDVDGDNQIDVAEYASCLM 64
Query: 366 NRMSGGSDEDDVVIN-AFKTFD--EEGKIDSERLRHALMTWGDKFSADEVDEAYDQMDID 536
G +++D+V+ AF +D +GKI + + L G+K + E +D D
Sbjct: 65 LGGEGNKEDEDIVMKEAFDLYDIDGDGKISASEIHVVLKRLGEKQTIAECIAMVRAVDAD 124
Query: 537 DKGYIDTTKLIAMLTAS 587
G++ + M++ +
Sbjct: 125 GDGFVSFEEFKTMMSCN 141
Score = 35.1 bits (77), Expect = 0.057
Identities = 13/57 (22%), Positives = 29/57 (50%)
Frame = +3
Query: 426 DEEGKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTASAEG 596
+++GKI + A+ + +++E+D + ++D+D ID + + L EG
Sbjct: 13 NKDGKISWDEFAEAIRAFSPSITSEEIDNMFREIDVDGDNQIDVAEYASCLMLGGEG 69
Score = 30.3 bits (65), Expect = 1.6
Identities = 24/82 (29%), Positives = 36/82 (43%), Gaps = 5/82 (6%)
Frame = +3
Query: 363 ANRMSGGSDEDDVVINAFKTFDEEG--KIDSERLRHALMTWGDKFSADE---VDEAYDQM 527
A R S + + N F+ D +G +ID LM G+ DE + EA+D
Sbjct: 26 AIRAFSPSITSEEIDNMFREIDVDGDNQIDVAEYASCLMLGGEGNKEDEDIVMKEAFDLY 85
Query: 528 DIDDKGYIDTTKLIAMLTASAE 593
DID G I +++ +L E
Sbjct: 86 DIDGDGKISASEIHVVLKRLGE 107
>At4g37010.1 68417.m05243 caltractin, putative / centrin, putative
similar to Caltractin (Centrin) SP:P41210 from [Atriplex
nummularia]
Length = 167
Score = 39.9 bits (89), Expect = 0.002
Identities = 18/70 (25%), Positives = 36/70 (51%), Gaps = 4/70 (5%)
Frame = +3
Query: 183 VAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQL 350
+ E +AF+++DHD +G I D++ LG ++ +++EM+ EA G +N +
Sbjct: 98 IDELSKAFKIIDHDNNGKISPRDIKMIAKELGENFTDNDIEEMIEEADRDKDGEVNLEEF 157
Query: 351 LTLFANRMSG 380
+ + G
Sbjct: 158 MKMMKRTSYG 167
>At3g03400.1 68416.m00337 calmodulin-related protein, putative
similar to calmodulin-related protein 2, touch-induced
SP:P25070 from [Arabidopsis thaliana]
Length = 137
Score = 39.5 bits (88), Expect = 0.003
Identities = 30/125 (24%), Positives = 53/125 (42%), Gaps = 7/125 (5%)
Frame = +3
Query: 198 EAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQLLTLF- 362
+ F+ D KDG I + R +L ++L EM + G ++ + +
Sbjct: 8 DIFERFDTSKDGKISWEEFRDAIHALSPSIPSEKLVEMFIQLDTNGDGQVDAAKFASCMD 67
Query: 363 ANRMSGGSDEDDVVINAFKTFDE--EGKIDSERLRHALMTWGDKFSADEVDEAYDQMDID 536
S G D + + +AFK +D +GKI + L + G+K + + +D+D
Sbjct: 68 QTAQSSGGDVEKELKDAFKLYDINCDGKISANELHVVMTRLGEKCTVESCVGMVQAIDVD 127
Query: 537 DKGYI 551
GYI
Sbjct: 128 GDGYI 132
Score = 35.1 bits (77), Expect = 0.057
Identities = 17/65 (26%), Positives = 33/65 (50%), Gaps = 2/65 (3%)
Frame = +3
Query: 414 FKTFD--EEGKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTAS 587
F+ FD ++GKI E R A+ ++++ E + Q+D + G +D K + + +
Sbjct: 10 FERFDTSKDGKISWEEFRDAIHALSPSIPSEKLVEMFIQLDTNGDGQVDAAKFASCMDQT 69
Query: 588 AEGGG 602
A+ G
Sbjct: 70 AQSSG 74
Score = 28.3 bits (60), Expect = 6.5
Identities = 15/42 (35%), Positives = 21/42 (50%)
Frame = +3
Query: 189 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 314
E K+AF+L D + DG I N+L LG + + MV
Sbjct: 80 ELKDAFKLYDINCDGKISANELHVVMTRLGEKCTVESCVGMV 121
>At3g25600.1 68416.m03187 calmodulin, putative similar to calmodulin
GI:239841 from [Paramecium tetraurelia]
Length = 161
Score = 39.1 bits (87), Expect = 0.003
Identities = 30/144 (20%), Positives = 63/144 (43%), Gaps = 7/144 (4%)
Frame = +3
Query: 180 QVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQ 347
Q+ + K+ F D DKDG + + +L A SLG ++ ++ + +G + F +
Sbjct: 9 QIKQLKDIFARFDMDKDGSLTQLELAALLRSLGIKPRSDQISLLLNQIDRNGNGSVEFDE 68
Query: 348 L-LTLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAY 518
L + + + + ++ F++FD + G I + L ++ G + E+ E
Sbjct: 69 LVVAILPDINEEVLINQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLTYRELTEMM 128
Query: 519 DQMDIDDKGYIDTTKLIAMLTASA 590
+ D + G I + ++ SA
Sbjct: 129 TEADSNGDGVISFNEFSHIMAKSA 152
>At1g73630.1 68414.m08524 calcium-binding protein, putative similar
to calcium binding protein GI:14589311 from [Sesbania
rostrata]; contains Pfam profile: PF00036 EF hand (4
copies)
Length = 163
Score = 39.1 bits (87), Expect = 0.003
Identities = 18/70 (25%), Positives = 35/70 (50%), Gaps = 4/70 (5%)
Frame = +3
Query: 171 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVG----EASGPIN 338
S E +EAF L D +K+G+I +++ + LG S ++ M+G + G +N
Sbjct: 83 SSSSAVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVDTDGDGNVN 142
Query: 339 FTQLLTLFAN 368
F + + ++
Sbjct: 143 FEEFQKMMSS 152
Score = 33.5 bits (73), Expect = 0.17
Identities = 16/61 (26%), Positives = 32/61 (52%), Gaps = 2/61 (3%)
Frame = +3
Query: 414 FKTFDE--EGKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTAS 587
F FD +GKI L + + G ++ +E++ D++DID G+I+ + + +S
Sbjct: 25 FDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFATICRSS 84
Query: 588 A 590
+
Sbjct: 85 S 85
>At2g36180.1 68415.m04440 calmodulin-related protein, putative
similar to calmodulin-related protein 2, touch-induced
SP:P25070 from [Arabidopsis thaliana]
Length = 144
Score = 37.9 bits (84), Expect = 0.008
Identities = 31/140 (22%), Positives = 63/140 (45%), Gaps = 8/140 (5%)
Frame = +3
Query: 198 EAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV----GEASGPINFTQLLTLFA 365
E F+ +D +KDG I ++ + +E+D+M + G I+ + +
Sbjct: 3 EIFESVDKNKDGKILWDEFAEAIRVFSPQITSEEIDKMFIVLDVDGDGQIDDVEFASCLM 62
Query: 366 NRMSGGSD-EDDVVIN-AFKTFDEEG--KIDSERLRHALMTWGDKFSADEVDEAYDQMDI 533
G D E++VV+ AF +D +G KI + + L G+K + ++ +D
Sbjct: 63 VNGGGEKDTEEEVVMKEAFDLYDMDGDGKISASEIHVVLKRLGEKHTMEDCVVMVQTVDK 122
Query: 534 DDKGYIDTTKLIAMLTASAE 593
D G+++ + M+ ++ E
Sbjct: 123 DSDGFVNFEEFKIMMNSNKE 142
Score = 33.5 bits (73), Expect = 0.17
Identities = 15/59 (25%), Positives = 33/59 (55%)
Frame = +3
Query: 426 DEEGKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTASAEGGG 602
+++GKI + A+ + + +++E+D+ + +D+D G ID + + L + GGG
Sbjct: 11 NKDGKILWDEFAEAIRVFSPQITSEEIDKMFIVLDVDGDGQIDDVEFASCLMVN--GGG 67
>At1g76640.1 68414.m08918 calmodulin-related protein, putative
similar to regulator of gene silencing
calmodulin-related protein GI:12963415 from [Nicotiana
tabacum]
Length = 159
Score = 37.5 bits (83), Expect = 0.011
Identities = 17/69 (24%), Positives = 35/69 (50%)
Frame = +3
Query: 387 DEDDVVINAFKTFDEEGKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKL 566
+ D + A+ + +G+I +E L+ + T G++ S +E + A DID G +D +
Sbjct: 20 NRDLEAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEF 79
Query: 567 IAMLTASAE 593
++ + E
Sbjct: 80 ALLIKGNDE 88
>At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains
ATP/GTP-binding site motif A (P-loop), PROSITE:PS00017
Length = 1340
Score = 37.1 bits (82), Expect = 0.014
Identities = 16/45 (35%), Positives = 26/45 (57%)
Frame = +3
Query: 189 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA 323
E +AF+ D ++ G + D+R T SLG+ S +E+ E+V A
Sbjct: 1274 ELLQAFRFFDRNQAGYVRVEDMRVTIHSLGKFLSHREVKELVQSA 1318
>At5g42380.1 68418.m05160 calmodulin-related protein, putative
similar to regulator of gene silencing
calmodulin-related protein GI:12963415 from [Nicotiana
tabacum]
Length = 185
Score = 35.9 bits (79), Expect = 0.032
Identities = 21/74 (28%), Positives = 35/74 (47%), Gaps = 6/74 (8%)
Frame = +3
Query: 375 SGGSDEDDVVINAFKT-FD-----EEGKIDSERLRHALMTWGDKFSADEVDEAYDQMDID 536
S S + +N +T FD +GKI E L+ + G S+ EV+E D+D
Sbjct: 37 SNSSGSSSLNVNELRTVFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVD 96
Query: 537 DKGYIDTTKLIAML 578
G+ID + + ++
Sbjct: 97 GDGFIDFEEFLKLM 110
>At5g66210.2 68418.m08341 calcium-dependent protein kinase family
protein / CDPK family protein contains Pfam domains,
PF00069: Protein kinase domain and PF00036: EF hand
Length = 523
Score = 35.1 bits (77), Expect = 0.057
Identities = 44/172 (25%), Positives = 79/172 (45%), Gaps = 12/172 (6%)
Frame = +3
Query: 108 RQSSRGSRKAKRTGSNVFSMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATF--DSLGR 281
RQ R SR + + S + ++++ ++ F +D DK+G+I ++R D +
Sbjct: 342 RQFVRYSRLKQFALRALASTLDEAEISDLRDQFDAIDVDKNGVISLEEMRQALAKDLPWK 401
Query: 282 LASEKE---LDEMVGEASGPINFTQLL--TLFANRMSGGSDEDDVVIN--AFKTF--DEE 434
L + L+ + G ++FT+ + L +++ E + + AF+ F D++
Sbjct: 402 LKDSRVAEILEAIDSNTDGLVDFTEFVAAALHVHQLEEHDSEKWQLRSRAAFEKFDLDKD 461
Query: 435 GKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAML-TAS 587
G I E LR M G + S +D D+ DID G I + +L TAS
Sbjct: 462 GYITPEELR---MHTGLRGS---IDPLLDEADIDRDGKISLHEFRRLLRTAS 507
>At5g66210.1 68418.m08340 calcium-dependent protein kinase family
protein / CDPK family protein contains Pfam domains,
PF00069: Protein kinase domain and PF00036: EF hand
Length = 523
Score = 35.1 bits (77), Expect = 0.057
Identities = 44/172 (25%), Positives = 79/172 (45%), Gaps = 12/172 (6%)
Frame = +3
Query: 108 RQSSRGSRKAKRTGSNVFSMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATF--DSLGR 281
RQ R SR + + S + ++++ ++ F +D DK+G+I ++R D +
Sbjct: 342 RQFVRYSRLKQFALRALASTLDEAEISDLRDQFDAIDVDKNGVISLEEMRQALAKDLPWK 401
Query: 282 LASEKE---LDEMVGEASGPINFTQLL--TLFANRMSGGSDEDDVVIN--AFKTF--DEE 434
L + L+ + G ++FT+ + L +++ E + + AF+ F D++
Sbjct: 402 LKDSRVAEILEAIDSNTDGLVDFTEFVAAALHVHQLEEHDSEKWQLRSRAAFEKFDLDKD 461
Query: 435 GKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAML-TAS 587
G I E LR M G + S +D D+ DID G I + +L TAS
Sbjct: 462 GYITPEELR---MHTGLRGS---IDPLLDEADIDRDGKISLHEFRRLLRTAS 507
>At4g04710.1 68417.m00692 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase [Nicotiana tabacum] gi|3283996|gb|AAC25423;
contains protein kinase domain, Pfam:PF00069; contains
EF hand domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 575
Score = 35.1 bits (77), Expect = 0.057
Identities = 30/126 (23%), Positives = 54/126 (42%), Gaps = 3/126 (2%)
Frame = +3
Query: 198 EAFQLMDHDKDGIIGKNDLRATFDS--LGRLASEKEL-DEMVGEASGPINFTQLLTLFAN 368
+AFQ D D G I K ++ +G A+ K+L E G I++ + T+ N
Sbjct: 413 KAFQYFDKDGSGHITKEEVEIAMKEHGMGDEANAKDLISEFDKNNDGKIDYEEFCTMMRN 472
Query: 369 RMSGGSDEDDVVINAFKTFDEEGKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGY 548
+ + ++ + +E L+ L G + S E+D+A+ D D+ G+
Sbjct: 473 GIL--QPQGKLLKRLYMNLEE--------LKTGLTRLGSRLSETEIDKAFQHFDKDNSGH 522
Query: 549 IDTTKL 566
I +L
Sbjct: 523 ITRDEL 528
>At1g21550.1 68414.m02695 calcium-binding protein, putative contains
similarity to calcium-binding protein GB:CAB63264
GI:6580549 from [Lotus japonicus]
Length = 155
Score = 34.7 bits (76), Expect = 0.075
Identities = 30/111 (27%), Positives = 54/111 (48%), Gaps = 13/111 (11%)
Frame = +3
Query: 189 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLA-SEKELDEMVGEASGPINFTQLLTLFA 365
+ + F+ +D ++DG++ ++L D LG + EL+ +VG+ S ++ + L +
Sbjct: 10 DLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQS--LDLDEFLRFYY 67
Query: 366 NRM--SGGS--------DEDDVVINAFKTFD--EEGKIDSERLRHALMTWG 482
+ + S GS D D+ + AF FD +G I +E LR L G
Sbjct: 68 DAVLDSKGSKKNIDVVADNDEAIARAFNVFDVNGDGYISAEELRDVLERLG 118
>At3g59440.1 68416.m06630 calcium-binding protein, putative similar
to calcium-binding protein [Lotus japonicus] GI:18413495
Length = 195
Score = 33.9 bits (74), Expect = 0.13
Identities = 21/72 (29%), Positives = 35/72 (48%), Gaps = 6/72 (8%)
Frame = +3
Query: 162 SMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLG-----RLASEKELDEMVGE-A 323
S+ +K+ + ++AF + D D DG I +L + SLG L KE+ V E
Sbjct: 114 SIVEEKEEGDMRDAFNVFDQDGDGFITVEELNSVMTSLGLKQGKTLECCKEMIMQVDEDG 173
Query: 324 SGPINFTQLLTL 359
G +N+ + L +
Sbjct: 174 DGRVNYKEFLQM 185
>At3g57530.1 68416.m06406 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calmodulin-domain protein
kinase CDPK isoform 7 [Arabidopsis thaliana]
gi|1399277|gb|AAB03247
Length = 538
Score = 33.9 bits (74), Expect = 0.13
Identities = 22/67 (32%), Positives = 33/67 (49%), Gaps = 3/67 (4%)
Frame = +3
Query: 384 SDEDDVVIN-AFKTFD--EEGKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYID 554
SDE+ I F+ D + GKI+ + L+ L G D++ D DID GY+D
Sbjct: 362 SDEEASGIREGFQIMDTSQRGKINIDELKIGLQKLGHAIPQDDLQILMDAGDIDRDGYLD 421
Query: 555 TTKLIAM 575
+ IA+
Sbjct: 422 CDEFIAI 428
>At4g03290.1 68417.m00449 calcium-binding protein, putative similar
to calcium-binding protein [Lotus japonicus]
GI:18413495; contains INTERPRO:IPR002048 calcium-binding
EF-hand domain
Length = 154
Score = 33.1 bits (72), Expect = 0.23
Identities = 17/40 (42%), Positives = 24/40 (60%)
Frame = +3
Query: 189 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDE 308
+ KEAF + D + DG I ++L+A SLG L K L+E
Sbjct: 81 DMKEAFNVFDRNGDGFITVDELKAVLSSLG-LKQGKTLEE 119
Score = 27.9 bits (59), Expect = 8.6
Identities = 15/57 (26%), Positives = 29/57 (50%), Gaps = 3/57 (5%)
Frame = +3
Query: 393 DDVVIN-AFKTFDEEG--KIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYID 554
D +N F+ FD++G KI ++ L + G DE+ + ++D++ G +D
Sbjct: 2 DSTELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVD 58
>At2g44310.1 68415.m05513 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 142
Score = 33.1 bits (72), Expect = 0.23
Identities = 17/67 (25%), Positives = 33/67 (49%)
Frame = +3
Query: 198 EAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANRMS 377
E F +D +KDG++ +++LR F+S+ L S +D + + + + F S
Sbjct: 27 ERFAALDLNKDGVLSRSELRKAFESMRLLESHFGVDVVTPQDELTNLYDSIFEKFDTDQS 86
Query: 378 GGSDEDD 398
G D ++
Sbjct: 87 GSVDLEE 93
>At5g39670.1 68418.m04804 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 204
Score = 32.7 bits (71), Expect = 0.30
Identities = 18/58 (31%), Positives = 32/58 (55%), Gaps = 2/58 (3%)
Frame = +3
Query: 111 QSSRGSRKAKRTGSNVFSMFSQKQ--VAEFKEAFQLMDHDKDGIIGKNDLRATFDSLG 278
Q + G +K + + V ++F +K+ + E K+AF + D ++DG I DL+ LG
Sbjct: 109 QENEGLQK-QYSSKEVSNLFEEKEPSLEEVKQAFDVFDENRDGFIDPIDLQRVLTILG 165
>At3g03410.1 68416.m00339 calmodulin-related protein, putative
similar to calmodulin-related protein 2, touch-induced
SP:P25070 from [Arabidopsis thaliana]
Length = 131
Score = 32.7 bits (71), Expect = 0.30
Identities = 25/132 (18%), Positives = 59/132 (44%), Gaps = 4/132 (3%)
Frame = +3
Query: 195 KEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKEL----DEMVGEASGPINFTQLLTLF 362
K F+ D +KDG + ++ R + +++++ +E+ + +G +N + +
Sbjct: 4 KRVFEKFDKNKDGKLSLDEFREVALAFSPYFTQEDIVKFFEEIDVDGNGELNADEFTSCI 63
Query: 363 ANRMSGGSDEDDVVINAFKTFDEEGKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDK 542
+ +V + F D +GKI + + + G KF+ + E D+D
Sbjct: 64 EKMLK------EVFV--FCDVDGDGKIPASESYVTMTSLGKKFTEETSAEKVRAADVDGD 115
Query: 543 GYIDTTKLIAML 578
GY++ + +A++
Sbjct: 116 GYLNFDEFMALV 127
>At1g18530.1 68414.m02312 calmodulin, putative similar to calmodulin
GI:1565285 from [Toxoplasma gondii]
Length = 157
Score = 32.7 bits (71), Expect = 0.30
Identities = 19/60 (31%), Positives = 30/60 (50%), Gaps = 4/60 (6%)
Frame = +3
Query: 198 EAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLLTLFA 365
E F+ D D +G I +L +G+ + KEL EM+ EA G I+F + ++ A
Sbjct: 85 EIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELTEMIKEADTNGDGVISFGEFASIMA 144
>At4g25970.1 68417.m03737 phosphatidylserine decarboxylase, putative
similar to SP|P53037 Phosphatidylserine decarboxylase
proenzyme 2 precursor (EC 4.1.1.65) {Saccharomyces
cerevisiae}; contains Pfam profile PF02666:
phosphatidylserine decarboxylase
Length = 635
Score = 32.3 bits (70), Expect = 0.40
Identities = 13/57 (22%), Positives = 29/57 (50%)
Frame = +3
Query: 423 FDEEGKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTASAE 593
+DE+GK+ + +G+ +A++ +E + D++ G + +L A+L E
Sbjct: 189 YDEDGKLSFSEFSDLMNAFGNVVAANKKEELFKAADLNGDGVVTIDELAALLAVQQE 245
>At3g29000.1 68416.m03624 calcium-binding EF hand family protein
similar to calmodulin-like MSS3 GI:9965747 from
[Arabidopsis thaliana]; contains INTERPRO:IPR002048
calcium-binding EF-hand domain
Length = 194
Score = 32.3 bits (70), Expect = 0.40
Identities = 16/47 (34%), Positives = 26/47 (55%), Gaps = 2/47 (4%)
Frame = +3
Query: 144 TGSNVFSMFSQKQVA--EFKEAFQLMDHDKDGIIGKNDLRATFDSLG 278
+ V S+F +K+ + E K+AF + D +KDG I +L+ LG
Sbjct: 109 SAKEVSSLFEEKEASLEEVKQAFDVFDENKDGFIDAIELQRVLTILG 155
>At3g47480.1 68416.m05163 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 183
Score = 31.9 bits (69), Expect = 0.53
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = +3
Query: 195 KEAFQLMDHDKDGIIGKNDLRATFDSLG 278
KEAF+L D ++DG I +N+L+ LG
Sbjct: 118 KEAFRLFDENQDGFIDENELKHVLSLLG 145
>At4g00310.1 68417.m00039 expressed protein
Length = 302
Score = 31.5 bits (68), Expect = 0.70
Identities = 22/60 (36%), Positives = 28/60 (46%), Gaps = 4/60 (6%)
Frame = +3
Query: 369 RMSGGSDEDDVVINAFKTFDEEGKIDSERLRHALMTW-GDKFSADE---VDEAYDQMDID 536
R GGSD++ +VIN K S+ H + G S DE VDE YD M+ D
Sbjct: 206 RKRGGSDDEKMVINDSKKSRSSASSSSDEDGHKTAAFGGGSVSFDEFDFVDEDYDVMEQD 265
>At2g43290.1 68415.m05382 calmodulin-like protein (MSS3) identical
to calmodulin-like MSS3 from GI:9965747 [Arabidopsis
thaliana]
Length = 215
Score = 31.5 bits (68), Expect = 0.70
Identities = 19/64 (29%), Positives = 32/64 (50%), Gaps = 7/64 (10%)
Frame = +3
Query: 189 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELD-------EMVGEASGPINFTQ 347
+ K+AF + D D DG I +L++ SLG L K LD ++ + G +N+ +
Sbjct: 143 DMKDAFNVFDQDGDGFITVEELKSVMASLG-LKQGKTLDGCKKMIMQVDADGDGRVNYKE 201
Query: 348 LLTL 359
L +
Sbjct: 202 FLQM 205
>At4g20780.1 68417.m03017 calcium-binding protein, putative similar
to SP|Q09011 Calcium-binding protein CAST {Solanum
tuberosum}; contains INTERPRO:IPR002048 calcium-binding
EF-hand domain
Length = 191
Score = 31.1 bits (67), Expect = 0.92
Identities = 43/174 (24%), Positives = 66/174 (37%), Gaps = 26/174 (14%)
Frame = +3
Query: 111 QSSRGSRKAKRTGSNVFSMFSQKQVA-EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLA 287
+S+ +K S+ F + S A + F L D + DG I +L LG A
Sbjct: 2 ESNNNEKKKVARQSSSFRLRSPSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNA 61
Query: 288 SEKELDEMVGEASGP----INFTQLLTL--------FANRMSGGSDEDDV---------V 404
+L V P +NF +L F GG +EDD +
Sbjct: 62 DLSDLKSTVESYIQPGNTGLNFDDFSSLHKTLDDSFFGGACGGGENEDDPSSAAENESDL 121
Query: 405 INAFKTFDE--EGKIDSERLRHALMTWG--DKFSADEVDEAYDQMDIDDKGYID 554
AFK FDE +G I + L+ L G + + V++ +D + G +D
Sbjct: 122 AEAFKVFDENGDGFISARELQTVLKKLGLPEGGEMERVEKMIVSVDRNQDGRVD 175
>At3g07490.1 68416.m00893 calcium-binding protein, putative similar
to calcium-binding protein GI:6580549 from [Lotus
japonicus]
Length = 153
Score = 31.1 bits (67), Expect = 0.92
Identities = 23/80 (28%), Positives = 39/80 (48%), Gaps = 7/80 (8%)
Frame = +3
Query: 165 MFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDE---MVG----EA 323
M + + + +EAF + D ++DG I +LR+ SLG L + L++ M+ +
Sbjct: 70 MEERDEEEDMREAFNVFDQNRDGFITVEELRSVLASLG-LKQGRTLEDCKRMISKVDVDG 128
Query: 324 SGPINFTQLLTLFANRMSGG 383
G +NF + F M GG
Sbjct: 129 DGMVNFKE----FKQMMKGG 144
>At4g20190.1 68417.m02952 hypothetical protein
Length = 389
Score = 30.7 bits (66), Expect = 1.2
Identities = 17/37 (45%), Positives = 23/37 (62%)
Frame = +3
Query: 378 GGSDEDDVVINAFKTFDEEGKIDSERLRHALMTWGDK 488
GG+D+DD V AF FD+E +D E ++ L T G K
Sbjct: 306 GGNDQDDPVSAAF-VFDKEPNLDKE-IKGVLKTSGSK 340
>At4g04740.1 68417.m00695 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase [Lycopersicon esculentum]
gi|19171502|emb|CAC87494
Length = 520
Score = 30.7 bits (66), Expect = 1.2
Identities = 17/54 (31%), Positives = 29/54 (53%), Gaps = 4/54 (7%)
Frame = +3
Query: 198 EAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQ 347
+AFQ +D DK+G I +++L + G + E + E++ E G INF +
Sbjct: 448 KAFQHLDKDKNGHITRDELESAMKEYG-MGDEASIKEVISEVDTDNDGKINFEE 500
>At4g02540.1 68417.m00347 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 822
Score = 30.3 bits (65), Expect = 1.6
Identities = 15/44 (34%), Positives = 26/44 (59%), Gaps = 1/44 (2%)
Frame = -1
Query: 406 ITTSSSSEPPDMRLANRVKS*VKLMGPEASPTISSSSFS-DASL 278
+ S+ SEPP+M+LA+ +K + L+ + P + S + DA L
Sbjct: 88 LVESTYSEPPEMKLASLIKKLMPLIDTQIPPIFTGKSLTYDAKL 131
>At3g51670.1 68416.m05666 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein similar to
polyphosphoinositide binding protein Ssh2p (GI:2739046)
{Glycine max};; contains Pfam PF00650 : CRAL/TRIO
domain; contains Pfam PF03765 : CRAL/TRIO, N-terminus
Length = 409
Score = 30.3 bits (65), Expect = 1.6
Identities = 17/74 (22%), Positives = 38/74 (51%)
Frame = +3
Query: 291 EKELDEMVGEASGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFDEEGKIDSERLRHAL 470
+K L E+ + S + ++ +++ + GG D+ DV++ F + DS R+
Sbjct: 49 QKSLQELKEKLSA--SSSKASSMWGVSLLGGDDKADVILLKFLRARDFKVADSLRMLEKC 106
Query: 471 MTWGDKFSADEVDE 512
+ W ++F A+++ E
Sbjct: 107 LEWREEFKAEKLTE 120
>At2g17890.1 68415.m02072 calcium-dependent protein kinase family
protein / CDPK family protein contains Pfam domains,
PF00069: Protein kinase domain and PF00036: EF hand
Length = 571
Score = 30.3 bits (65), Expect = 1.6
Identities = 42/172 (24%), Positives = 77/172 (44%), Gaps = 12/172 (6%)
Frame = +3
Query: 108 RQSSRGSRKAKRTGSNVFSMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATF--DSLGR 281
RQ + SR + + + ++++A+ ++ F +D DK+G+I ++R D +
Sbjct: 388 RQFVKFSRLKQFALRALATTLDEEELADLRDQFDAIDVDKNGVISLEEMRQALAKDHPWK 447
Query: 282 LASEKE---LDEMVGEASGPINFTQLL--TLFANRMSGGSDE--DDVVINAFKTFDEEGK 440
L + L + G ++F + + L N++ E AF+ FD +G
Sbjct: 448 LKDARVAEILQAIDSNTDGFVDFGEFVAAALHVNQLEEHDSEKWQQRSRAAFEKFDIDGD 507
Query: 441 --IDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAML-TAS 587
I +E LR M G K S ++ ++ DID+ G I + +L TAS
Sbjct: 508 GFITAEELR---MHTGLKGS---IEPLLEEADIDNDGKISLQEFRRLLRTAS 553
>At2g41860.2 68415.m05174 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calmodulin-domain protein
kinase CDPK isoform 7 [Arabidopsis thaliana]
gi|1399277|gb|AAB03247
Length = 530
Score = 29.9 bits (64), Expect = 2.1
Identities = 17/65 (26%), Positives = 27/65 (41%), Gaps = 2/65 (3%)
Frame = +3
Query: 387 DEDDVVINAFKTFD--EEGKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTT 560
+E + F+ D GKI L L G D++ D D+D GY+D
Sbjct: 355 EETSCIKERFQVMDTSNRGKITITELGIGLQKLGIVVPQDDIQILMDAGDVDKDGYLDVN 414
Query: 561 KLIAM 575
+ +A+
Sbjct: 415 EFVAI 419
>At2g41860.1 68415.m05173 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calmodulin-domain protein
kinase CDPK isoform 7 [Arabidopsis thaliana]
gi|1399277|gb|AAB03247
Length = 425
Score = 29.9 bits (64), Expect = 2.1
Identities = 17/65 (26%), Positives = 27/65 (41%), Gaps = 2/65 (3%)
Frame = +3
Query: 387 DEDDVVINAFKTFD--EEGKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTT 560
+E + F+ D GKI L L G D++ D D+D GY+D
Sbjct: 250 EETSCIKERFQVMDTSNRGKITITELGIGLQKLGIVVPQDDIQILMDAGDVDKDGYLDVN 309
Query: 561 KLIAM 575
+ +A+
Sbjct: 310 EFVAI 314
>At5g25630.1 68418.m03050 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 574
Score = 29.5 bits (63), Expect = 2.8
Identities = 20/49 (40%), Positives = 26/49 (53%)
Frame = +3
Query: 288 SEKELDEMVGEASGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFDEE 434
+E+ L M G P N T LL A R++G +DE + INA K D E
Sbjct: 453 AEEVLQMMRGCGVKPENSTFLLLAEAWRVAGLTDESNKAINALKCKDIE 501
>At5g51600.1 68418.m06397 microtubule associated protein
(MAP65/ASE1) family protein low similarity to SP|P50275
Anaphase spindle elongation protein {Saccharomyces
cerevisiae}, protein regulating cytokinesis 1 (PRC1)
[Homo sapiens] GI:2865521; contains Pfam profile
PF03999: Microtubule associated protein (MAP65/ASE1
family)
Length = 707
Score = 29.1 bits (62), Expect = 3.7
Identities = 13/25 (52%), Positives = 18/25 (72%)
Frame = -3
Query: 368 VGEQSQELSEVDGARSLTDHLIELL 294
VG+ + LS+ +G RSL+DH IE L
Sbjct: 206 VGQVNPTLSDPEGPRSLSDHTIEKL 230
>At4g04700.1 68417.m00690 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase [Nicotiana tabacum] gi|3283996|gb|AAC25423;
contains protein kinase domain, Pfam:PF00069
Length = 485
Score = 29.1 bits (62), Expect = 3.7
Identities = 19/64 (29%), Positives = 28/64 (43%), Gaps = 4/64 (6%)
Frame = +3
Query: 198 EAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLLTLFA 365
+AFQ D D DG I K +L G E + +++ +A G INF + T+
Sbjct: 411 KAFQHFDKDNDGHITKEELEMAMKEDG-AGDEGSIKQIIADADTDNDGKINFEEFRTMMR 469
Query: 366 NRMS 377
S
Sbjct: 470 TESS 473
>At3g43480.1 68416.m04609 hypothetical protein hypothetical protein
At2g15180 - Arabidopsis thaliana, EMBL:AC006298
Length = 161
Score = 29.1 bits (62), Expect = 3.7
Identities = 9/19 (47%), Positives = 14/19 (73%)
Frame = +1
Query: 649 DASRSHHARPRFTRIKHHH 705
D +SHH +P+ +R+K HH
Sbjct: 116 DVPKSHHFQPKLSRLKTHH 134
>At3g43470.1 68416.m04608 hypothetical protein
Length = 458
Score = 29.1 bits (62), Expect = 3.7
Identities = 9/19 (47%), Positives = 14/19 (73%)
Frame = +1
Query: 649 DASRSHHARPRFTRIKHHH 705
D +SHH +P+ +R+K HH
Sbjct: 413 DVPKSHHFQPKLSRLKTHH 431
>At2g34210.1 68415.m04186 KOW domain-containing transcription factor
family protein
Length = 990
Score = 29.1 bits (62), Expect = 3.7
Identities = 24/72 (33%), Positives = 30/72 (41%)
Frame = +3
Query: 192 FKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANR 371
FK+ F I +N + TFD L R E +G I+F TLFANR
Sbjct: 358 FKDGFLYKKVSTKSIAAQN-VTPTFDELERFKRPNE--------NGEIDFVDESTLFANR 408
Query: 372 MSGGSDEDDVVI 407
G + D VI
Sbjct: 409 KKGHFMKGDAVI 420
>At2g15185.1 68415.m01732 zinc knuckle (CCHC-type) protein-related
supported by tandem duplication of zinc knuckle (CCHC
type) familyprotein (GI:4585917) (TIGR_Ath1:At2g15180)
[Arabidopsis thaliana]
Length = 191
Score = 29.1 bits (62), Expect = 3.7
Identities = 9/19 (47%), Positives = 14/19 (73%)
Frame = +1
Query: 649 DASRSHHARPRFTRIKHHH 705
D +SHH +P+ +R+K HH
Sbjct: 146 DVPKSHHFQPKLSRLKTHH 164
>At1g54530.1 68414.m06219 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 127
Score = 28.7 bits (61), Expect = 4.9
Identities = 22/79 (27%), Positives = 37/79 (46%), Gaps = 11/79 (13%)
Frame = +3
Query: 204 FQLMDHDKDGIIGKNDLRATFDSLGRLASE----KELD-------EMVGEASGPINFTQL 350
FQ++D DK+G++ ++LR +++ + SE E D E GE P NF L
Sbjct: 29 FQILDLDKNGMLSPSELRQGLNNVVAVESEVAPGDETDNVYNAIFERFGEDLVPKNFRDL 88
Query: 351 LTLFANRMSGGSDEDDVVI 407
+ M+ G V++
Sbjct: 89 IAEILTAMARGIGNSPVIM 107
>At1g13800.1 68414.m01620 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 883
Score = 28.7 bits (61), Expect = 4.9
Identities = 25/85 (29%), Positives = 39/85 (45%), Gaps = 10/85 (11%)
Frame = +3
Query: 129 RKAKRTGSNVFSMFSQK--QVAEFKEAFQLMDHDKDGIIGKNDL--RATFDSLGRLASEK 296
+K KR + S Q Q+ F EA+ L ++ I + + FD+LG+L +
Sbjct: 354 KKRKRINCVIVSSILQCYCQMGNFSEAYDLFKEFRETNISLDRVCYNVAFDALGKLGKVE 413
Query: 297 E----LDEMVGEASGP--INFTQLL 353
E EM G+ P IN+T L+
Sbjct: 414 EAIELFREMTGKGIAPDVINYTTLI 438
>At3g10300.3 68416.m01236 calcium-binding EF hand family protein low
similarity to SP|P12815 Programmed cell death protein 6
(Probable calcium-binding protein ALG-2) {Mus musculus};
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 335
Score = 28.3 bits (60), Expect = 6.5
Identities = 20/62 (32%), Positives = 30/62 (48%), Gaps = 1/62 (1%)
Frame = +3
Query: 138 KRTGSNVF-SMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 314
++ G F S+F Q ++ F+ D D+ G I N+LR SLG S LD +V
Sbjct: 218 RKIGPKEFTSLFFSLQ--NWRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLV 275
Query: 315 GE 320
+
Sbjct: 276 SK 277
>At3g10300.2 68416.m01235 calcium-binding EF hand family protein low
similarity to SP|P12815 Programmed cell death protein 6
(Probable calcium-binding protein ALG-2) {Mus musculus};
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 324
Score = 28.3 bits (60), Expect = 6.5
Identities = 20/62 (32%), Positives = 30/62 (48%), Gaps = 1/62 (1%)
Frame = +3
Query: 138 KRTGSNVF-SMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 314
++ G F S+F Q ++ F+ D D+ G I N+LR SLG S LD +V
Sbjct: 218 RKIGPKEFTSLFFSLQ--NWRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLV 275
Query: 315 GE 320
+
Sbjct: 276 SK 277
>At5g51440.1 68418.m06377 23.5 kDa mitochondrial small heat shock
protein (HSP23.5-M) similar to heat shock 22 kDa
protein, mitochondrial precursor SP:Q96331 from
[Arabidopsis thaliana]; identified in Scharf, K-D., et
al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 210
Score = 27.9 bits (59), Expect = 8.6
Identities = 13/31 (41%), Positives = 22/31 (70%), Gaps = 2/31 (6%)
Frame = +2
Query: 200 GIPANGSRQGWHHRQERLARHLR--LPGQAR 286
G+ A+G R+GW+ +++ A HLR +PG +R
Sbjct: 101 GMGASGVRRGWNVKEKDDALHLRIDMPGLSR 131
>At2g19390.1 68415.m02262 expressed protein
Length = 1211
Score = 27.9 bits (59), Expect = 8.6
Identities = 10/29 (34%), Positives = 17/29 (58%)
Frame = +1
Query: 217 ITTRMASSARTTCAPPSTPWAGSRPRRSS 303
+T R ++ + +PP T WA RP++ S
Sbjct: 440 VTQRKRMTSNRSSSPPVTQWASQRPQKIS 468
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,466,717
Number of Sequences: 28952
Number of extensions: 238510
Number of successful extensions: 981
Number of sequences better than 10.0: 88
Number of HSP's better than 10.0 without gapping: 859
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 932
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1882599200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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