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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30841
         (748 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||...   457   e-130
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb...   260   1e-70
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s...   248   9e-67
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce...   173   2e-44
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe...   121   1e-28
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy...   113   3e-26
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc...   100   3e-22
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po...    76   6e-15
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa...    68   2e-12
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb...    38   0.002
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr...    31   0.17 
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce...    30   0.30 
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom...    27   3.8  
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p...    26   5.0  

>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 375

 Score =  457 bits (1127), Expect = e-130
 Identities = 209/230 (90%), Positives = 224/230 (97%)
 Frame = +1

Query: 55  DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 234
           ++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK
Sbjct: 2   EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61

Query: 235 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 414
           RGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQ
Sbjct: 62  RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121

Query: 415 IMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLA 594
           I+FETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIYEGYALPHAI+RLDLA
Sbjct: 122 IIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRLDLA 181

Query: 595 GRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAA 744
           GRDLTDYLMKIL ERGY+F+TTAEREIVRDIKEKLCYVALDFEQE+ TAA
Sbjct: 182 GRDLTDYLMKILMERGYTFSTTAEREIVRDIKEKLCYVALDFEQELQTAA 231


>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 379

 Score =  260 bits (638), Expect = 1e-70
 Identities = 119/225 (52%), Positives = 164/225 (72%), Gaps = 1/225 (0%)
 Frame = +1

Query: 61  DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 240
           D   + +DNGSG  KAGFAGDD P+ +FP+ VGR +H+ VM    QKD +VG EAQ+ RG
Sbjct: 8   DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67

Query: 241 ILTLKYPIEHGIITNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTQI 417
           +L ++ PIE GII NW DME+IW + + ++ L   PEEHP+LLTE PL    N+EK+ + 
Sbjct: 68  LLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEY 127

Query: 418 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAG 597
            +ET N PA+  ++Q VL+LYAS RTTGIVL+ GDG++H+VPIY+G+++P AI + ++ G
Sbjct: 128 FYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAIQQEEIGG 187

Query: 598 RDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEM 732
           RD+TDYL   L + G+   ++AE+EIVR+IKEK CYVA DF  E+
Sbjct: 188 RDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYVASDFRSEI 232


>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
           subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 390

 Score =  248 bits (606), Expect = 9e-67
 Identities = 115/221 (52%), Positives = 160/221 (72%), Gaps = 2/221 (0%)
 Frame = +1

Query: 67  AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRG 240
           A +V+DNG+G  K G+A D+ PR  FPSIVGRP  R +     +  KD  VGDEA++ R 
Sbjct: 4   APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63

Query: 241 ILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIM 420
           +L +KYP+E+GII ++++M ++W +TF+ +L++ P    +LLTE P+NP ANREKM + M
Sbjct: 64  LLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETM 123

Query: 421 FETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGR 600
           FE +    +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE   L H + RLD+AGR
Sbjct: 124 FERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVGRLDVAGR 183

Query: 601 DLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFE 723
           D T YL+ +L  +GY+F  TA+ E VR++KEKLCYV+ D E
Sbjct: 184 DATRYLISLLLRKGYAFNRTADFETVREMKEKLCYVSYDLE 224


>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 427

 Score =  173 bits (421), Expect = 2e-44
 Identities = 103/255 (40%), Positives = 143/255 (56%), Gaps = 34/255 (13%)
 Frame = +1

Query: 73  LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 201
           +++DNG+G  K G+AG+DAP  VFP+++   R  G   G         M  K        
Sbjct: 8   IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66

Query: 202 ------DSYVGDEAQSKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 360
                 D ++G++A  K     +L YPI HG I NWD ME+ W  + +  LR  PE+H  
Sbjct: 67  RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126

Query: 361 LLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRT--------TGIVLDS 516
           LLTE PLNP  NRE   +IMFE+FN   +Y+A+QAVL+L AS  +        TG V+DS
Sbjct: 127 LLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDS 186

Query: 517 GDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGY--SFTTTAEREIVRDIK 690
           GDGV+H +P+ EGY +  +I  + LAGRD+T ++  +L +R    S   TAER     IK
Sbjct: 187 GDGVTHIIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDRNEPDSSLKTAER-----IK 241

Query: 691 EKLCYVALDFEQEMA 735
           E+ CYV  D  +E +
Sbjct: 242 EECCYVCPDIVKEFS 256


>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 721

 Score =  121 bits (291), Expect = 1e-28
 Identities = 72/249 (28%), Positives = 132/249 (53%), Gaps = 3/249 (1%)
 Frame = +1

Query: 4   ESIPASPTQTHTKFKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVM 183
           E + + PT +   F+   +D+  LV+DNGS   +AG+ G+  P+ VF ++V R R +   
Sbjct: 8   EPVFSGPTPS---FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDRK-- 61

Query: 184 VGMGQKDSYVGDEAQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 360
             + +  + VG++   + G  ++ + P E  +I+NWD ME++  +TF  +L +   EHP+
Sbjct: 62  --LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPI 118

Query: 361 LLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSH 534
            +TE   NP   R  MT+++FE +N+P++   I  + S Y + +  ++GIVL+ G+  SH
Sbjct: 119 CMTEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASH 178

Query: 535 TVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVAL 714
            +P+  G  +     R+   G   + YL+K+   +  SF           +    C+V+ 
Sbjct: 179 VIPVLNGERILSEAKRISWGGSQSSSYLLKLFQIKYPSFPIKMLPSQAELLMHDHCHVSS 238

Query: 715 DFEQEMATA 741
           D+  ++A A
Sbjct: 239 DYTHDIAHA 247


>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 433

 Score =  113 bits (271), Expect = 3e-26
 Identities = 63/196 (32%), Positives = 101/196 (51%)
 Frame = +1

Query: 58  DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 237
           D+V+A+V+D GS   + GF+G+D P+ V PS  G       + G    + Y+    +S  
Sbjct: 9   DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNP 61

Query: 238 GILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQI 417
           G + +K  I +G + NWD    +W +    +L+  P EHP+L+TE   NP  NR K  + 
Sbjct: 62  G-MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLET 120

Query: 418 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAG 597
           MFE+   PA Y+A Q   + +ASG+ T  ++D G   S    IY+G+ L         +G
Sbjct: 121 MFESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVLQKGYQVQHFSG 180

Query: 598 RDLTDYLMKILTERGY 645
             + D L + L ++ +
Sbjct: 181 NAINDILAQTLRDKNF 196


>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
           Arp42|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 430

 Score =  100 bits (239), Expect = 3e-22
 Identities = 54/200 (27%), Positives = 103/200 (51%), Gaps = 2/200 (1%)
 Frame = +1

Query: 58  DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 237
           +++ +LV+D GS   + G+AG+++P  + PS      + GV   +  ++ YV DE Q   
Sbjct: 8   EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61

Query: 238 GI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 411
            I  + +K    +GII +W+     W      +L+V P E+ +++TE   NP++ R+++ 
Sbjct: 62  PIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIM 121

Query: 412 QIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDL 591
           +  FE  + PA Y+  QAV   +A+ ++T +++D G   +   P+ +G  +   I +  L
Sbjct: 122 EAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIGSDNASVTPVVDGLIIRKGIFKQSL 181

Query: 592 AGRDLTDYLMKILTERGYSF 651
           AG  L   + ++       F
Sbjct: 182 AGDFLNANIEQLFNTMNIEF 201


>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 401

 Score = 75.8 bits (178), Expect = 6e-15
 Identities = 62/238 (26%), Positives = 110/238 (46%), Gaps = 18/238 (7%)
 Frame = +1

Query: 73  LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 252
           +V+DNG+   KAGFAG        P+ + R +        G +  ++G+E  +     TL
Sbjct: 8   IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSKD-------GNR-LFLGNELANCNDFTTL 57

Query: 253 KYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEAPLNPKANREKMTQIM 420
           ++   H  G + +W     +W     N   + P   ++ +LLT+      +      Q++
Sbjct: 58  QFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQPVFTMPSIEHNTIQLV 117

Query: 421 FETFNSPAMYVAIQAVLSLYASGRTT--------------GIVLDSGDGVSHTVPIYEGY 558
           FE F   A      A L  +  G  T               +V+DSG   +H +P+ +  
Sbjct: 118 FEEFQFDAYLPCTPAELIPWDHGSFTMNQEDAYTGQHGECVLVIDSGYSFTHIIPVIDFS 177

Query: 559 ALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEM 732
               A+ R+D+ G+ LT+YL ++++ R Y+     E  IV +IKE +CYV+ +F+++M
Sbjct: 178 VQEQAVKRVDVGGKLLTNYLKEVISYRKYNM--MEETYIVNEIKESVCYVSQNFKEDM 233


>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
           Arp9|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 523

 Score = 67.7 bits (158), Expect = 2e-12
 Identities = 43/149 (28%), Positives = 70/149 (46%), Gaps = 4/149 (2%)
 Frame = +1

Query: 259 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 426
           PI+ G + +W+ ++  W H  Y+ L   P +    +PV L         +RE  TQ  FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171

Query: 427 TFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDL 606
               P   +A + ++ LYA G   G+V+D G   +   PI +G  +  A  +L L GR +
Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLGGRYM 231

Query: 607 TDYLMKILTERGYSFTTTAEREIVRDIKE 693
           T +L  +L E   +  ++ +     DI E
Sbjct: 232 TQHLQNLLRESLPTLKSSGQYVSKEDITE 260


>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 620

 Score = 37.9 bits (84), Expect = 0.002
 Identities = 28/146 (19%), Positives = 67/146 (45%), Gaps = 12/146 (8%)
 Frame = +1

Query: 250 LKYPIEHGIITNWD---------DMEKIWHHTFYNELRVAPEE---HPVLLTEAPLNPKA 393
           L YPI+ G+    D         D+ +I+ ++  + L++   +   + V+     L  + 
Sbjct: 198 LFYPIKSGVFNESDYASSQQLLADIYEIFKYSITSLLQIPVSQLSQYSVIFIVPDLYDRV 257

Query: 394 NREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHA 573
             EK+  I+F   +     +  +++ + + +G +   V+D G   +    + EG  +P++
Sbjct: 258 YVEKILDILFFDLHFGKAAIVQESLCTSFGAGMSAACVVDMGAQKTSISCVEEGVVVPNS 317

Query: 574 ILRLDLAGRDLTDYLMKILTERGYSF 651
            ++++  G D+T   MK+L    + +
Sbjct: 318 RIKINYGGDDITLLFMKLLMRSHFPY 343


>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1517

 Score = 31.1 bits (67), Expect = 0.17
 Identities = 16/48 (33%), Positives = 26/48 (54%)
 Frame = +1

Query: 118 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 261
           G+  PRA F  ++  P H G+++ M  KD   G+E  S +G  + + P
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547


>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1666

 Score = 30.3 bits (65), Expect = 0.30
 Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
 Frame = +1

Query: 319  FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 462
            +Y  L    E+HP+LLT+  A L P+ +  ++ +I  ++ N+P +    VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451


>SPAC1296.04 |mug65||spore wall assembly protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 248

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 9/16 (56%), Positives = 9/16 (56%)
 Frame = +2

Query: 545 STKVTLCPTPSSVWTW 592
           STK   CP PS  W W
Sbjct: 52  STKTATCPDPSWEWAW 67


>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 226

 Score = 26.2 bits (55), Expect = 5.0
 Identities = 10/25 (40%), Positives = 18/25 (72%)
 Frame = +1

Query: 361 LLTEAPLNPKANREKMTQIMFETFN 435
           +L  APL+ +   +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,899,082
Number of Sequences: 5004
Number of extensions: 58968
Number of successful extensions: 223
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 197
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 212
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 355273338
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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