BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30830 (758 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC328.10c |rps502|rps5-2|40S ribosomal protein S5|Schizosaccha... 265 5e-72 SPAC8C9.08 |rps5||40S ribosomal protein S5|Schizosaccharomyces p... 262 3e-71 SPAC16E8.10c |||mitochondrial ribosomal protein subunit S7|Schiz... 44 3e-05 SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 29 0.72 SPBC2G5.03 |||ATP binding protein|Schizosaccharomyces pombe|chr ... 27 2.2 SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces pombe... 27 2.9 SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 27 3.8 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 26 6.7 SPCC736.08 |cbf11||CBF1/Su|Schizosaccharomyces pombe|chr 3|||Manual 25 8.9 >SPAC328.10c |rps502|rps5-2|40S ribosomal protein S5|Schizosaccharomyces pombe|chr 1|||Manual Length = 203 Score = 265 bits (649), Expect = 5e-72 Identities = 135/199 (67%), Positives = 162/199 (81%) Frame = +1 Query: 76 AGSVVVETMSLPQAADIPEIKLFGRWSCYDVQVSDMSLQDYISVKEKYAKYLPHSAGRYA 255 A S++ + +SL + IKLF ++ V+V D+SL DYI++ + LPH+AGR+ Sbjct: 2 AASIIPKEVSLDETG---HIKLFNKFPFEGVEVKDISLVDYITIGN--GQPLPHTAGRFQ 56 Query: 256 HKRFRKAQCPIVERLTNSLMMHGRNNGKKLMAVRIVKHAFEIIHLLTGENPLQVLVTAII 435 KRFRKA+C IVERLTNSLMM+GRNNGKKL+A RIVKHAFEII LLT +NPLQVLV A+ Sbjct: 57 TKRFRKARCFIVERLTNSLMMNGRNNGKKLLATRIVKHAFEIIALLTDQNPLQVLVDAVA 116 Query: 436 NSGPREDSTRIGRAGTVRRQAVDVSPLRRVNQAIWLLCTGAREAAFRNIKTIAECVADEL 615 GPREDSTRIG AGTVRRQAVDVSPLRRVNQA+ L+ GAREAAFRN+K+I+EC+A+E+ Sbjct: 117 ACGPREDSTRIGSAGTVRRQAVDVSPLRRVNQALALITIGAREAAFRNVKSISECLAEEI 176 Query: 616 INAAKGSSNSYAIKKKDEL 672 INAAKGSSNSYAIKKKDEL Sbjct: 177 INAAKGSSNSYAIKKKDEL 195 >SPAC8C9.08 |rps5||40S ribosomal protein S5|Schizosaccharomyces pombe|chr 1|||Manual Length = 203 Score = 262 bits (643), Expect = 3e-71 Identities = 135/195 (69%), Positives = 158/195 (81%), Gaps = 3/195 (1%) Frame = +1 Query: 97 TMSLPQAADIPE---IKLFGRWSCYDVQVSDMSLQDYISVKEKYAKYLPHSAGRYAHKRF 267 T SL + E IKLF ++ V+V D+SL DYI++ + LPH+AGR+ KRF Sbjct: 3 TSSLTPGVSLDENGSIKLFNKFPFEGVEVKDISLVDYITIGN--GQPLPHTAGRFQTKRF 60 Query: 268 RKAQCPIVERLTNSLMMHGRNNGKKLMAVRIVKHAFEIIHLLTGENPLQVLVTAIINSGP 447 RKA+C IVERLTNSLMM+GRNNGKKL+A RIVKHAFEII LLT +NPLQVLV A+ GP Sbjct: 61 RKARCFIVERLTNSLMMNGRNNGKKLLATRIVKHAFEIIALLTDQNPLQVLVDAVAACGP 120 Query: 448 REDSTRIGRAGTVRRQAVDVSPLRRVNQAIWLLCTGAREAAFRNIKTIAECVADELINAA 627 REDSTRIG AGTVRRQAVDVSPLRRVNQA+ L+ GAREAAFRN+K+I+EC+A+E+INAA Sbjct: 121 REDSTRIGSAGTVRRQAVDVSPLRRVNQALALITIGAREAAFRNVKSISECLAEEIINAA 180 Query: 628 KGSSNSYAIKKKDEL 672 KGSSNSYAIKKKDEL Sbjct: 181 KGSSNSYAIKKKDEL 195 >SPAC16E8.10c |||mitochondrial ribosomal protein subunit S7|Schizosaccharomyces pombe|chr 1|||Manual Length = 259 Score = 43.6 bits (98), Expect = 3e-05 Identities = 40/133 (30%), Positives = 58/133 (43%), Gaps = 1/133 (0%) Frame = +1 Query: 289 VERLTNSLMMHGRNNGKKLMAVRIVKHAFEIIHLLTGENPLQVLVTAIINSGPREDSTRI 468 V+ L N +M +GKK A +IV A II TGENP+ VL AI P Sbjct: 126 VQHLVNLIM----RDGKKAKAEKIVATALSIIQKETGENPIDVLKQAIAEISPLMKLVSA 181 Query: 469 GRAGTVRRQAVDVSPLRRVNQAI-WLLCTGAREAAFRNIKTIAECVADELINAAKGSSNS 645 R + + +R A+ W+L E + K +++ + E+I +SN Sbjct: 182 KRFNKSVEFPMPLKERQRRRIALQWIL----GECKSSSPKRLSDRIVKEIIAIRSKTSNC 237 Query: 646 YAIKKKDELGACC 684 + KKKD L C Sbjct: 238 F--KKKDHLHRMC 248 >SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 468 Score = 29.1 bits (62), Expect = 0.72 Identities = 14/33 (42%), Positives = 17/33 (51%) Frame = +3 Query: 348 GRTYCQTCV*NYSLVNWRKPSASTRDCHYQLWT 446 G TYC C L+NW K S S C +L+T Sbjct: 101 GHTYCYEC-----LLNWLKESKSCPTCRQKLYT 128 >SPBC2G5.03 |||ATP binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 335 Score = 27.5 bits (58), Expect = 2.2 Identities = 17/51 (33%), Positives = 28/51 (54%) Frame = +1 Query: 370 AFEIIHLLTGENPLQVLVTAIINSGPREDSTRIGRAGTVRRQAVDVSPLRR 522 + +I HL+TG N + T ++N R D R+ R+ + Q+ D SP +R Sbjct: 159 SLDIHHLVTGHNADDIAETILMNL-LRGDVARLPRSTEITTQS-DSSPTKR 207 >SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 27.1 bits (57), Expect = 2.9 Identities = 16/48 (33%), Positives = 27/48 (56%) Frame = +1 Query: 112 QAADIPEIKLFGRWSCYDVQVSDMSLQDYISVKEKYAKYLPHSAGRYA 255 + ADI + FGR ++++++ + I+V+EKYAK P R A Sbjct: 7 KVADI-SLAAFGR---KELEIAENEMPGLIAVREKYAKSQPLKGARIA 50 >SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosaccharomyces pombe|chr 2|||Manual Length = 933 Score = 26.6 bits (56), Expect = 3.8 Identities = 12/39 (30%), Positives = 21/39 (53%) Frame = +2 Query: 440 LDPVKIRLGSVVRVQFVVKPLMFHPCAESTKQSGFCAQV 556 ++PV R SVV V +P+ +H +S ++G Q+ Sbjct: 273 IEPVSSRQSSVVNNNSVQQPVAYHAFVQSPTENGTLPQL 311 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 25.8 bits (54), Expect = 6.7 Identities = 12/41 (29%), Positives = 22/41 (53%) Frame = +3 Query: 297 PYKLSNDARSEQWQKTDGRTYCQTCV*NYSLVNWRKPSAST 419 PYK+ + + W + G+TY + + + L R+ +AST Sbjct: 138 PYKIVEHSNGDAWLEARGKTYSPSQIGGFILSKMRE-TAST 177 >SPCC736.08 |cbf11||CBF1/Su|Schizosaccharomyces pombe|chr 3|||Manual Length = 613 Score = 25.4 bits (53), Expect = 8.9 Identities = 16/54 (29%), Positives = 28/54 (51%) Frame = -2 Query: 274 LYGNACVHTCQLNEVNILRTSL*RKCSPAETYQRLAHRSNSICRKA*FQECRRL 113 L N C+ LNE ++ + SL R P++ ++ R +S+ +K+ E R L Sbjct: 147 LLNNCCIGIANLNEDSVHKISLTRSGKPSQLV-TVSCRHSSVIQKSYGSEKRYL 199 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,052,572 Number of Sequences: 5004 Number of extensions: 62695 Number of successful extensions: 161 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 147 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 159 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 363302114 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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