Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30823
(805 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g23930.1 68416.m03006 expressed protein 33 0.22
At1g05320.1 68414.m00539 myosin-related similar to non-muscle my... 33 0.29
At2g18540.1 68415.m02160 cupin family protein contains Pfam prof... 32 0.51
At1g08730.1 68414.m00969 myosin heavy chain (PCR43) identical to... 32 0.51
At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / hea... 29 2.7
At5g25060.1 68418.m02970 RNA recognition motif (RRM)-containing ... 29 2.7
At2g39300.1 68415.m04825 expressed protein ; expression supporte... 29 2.7
At5g41540.1 68418.m05048 disease resistance protein (TIR-NBS-LRR... 29 4.8
At5g14390.1 68418.m01681 expressed protein 29 4.8
At3g43160.1 68416.m04554 expressed protein merozoite surface pro... 28 8.3
At2g33990.1 68415.m04162 calmodulin-binding family protein simil... 28 8.3
At2g25350.1 68415.m03032 phox (PX) domain-containing protein wea... 28 8.3
>At3g23930.1 68416.m03006 expressed protein
Length = 224
Score = 33.1 bits (72), Expect = 0.22
Identities = 15/40 (37%), Positives = 21/40 (52%)
Frame = +1
Query: 175 KPAPKQEGEGDPEFIKRQDQKRSDLDEQLKEYINEWRKQR 294
K K + G IK +D++ + QLKE EWRK+R
Sbjct: 17 KETSKDQSRGRRHLIKERDEREKVMFLQLKEAEREWRKER 56
>At1g05320.1 68414.m00539 myosin-related similar to non-muscle
myosin II heavy chain (GI:19879404) [Loligo pealei];
ESTs gb|AA042402,gb|ATTS1380 come from this gene
Length = 828
Score = 32.7 bits (71), Expect = 0.29
Identities = 19/74 (25%), Positives = 36/74 (48%)
Frame = +1
Query: 445 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLERG 624
+E+K + LEE +K + A+ A + G +IQ + +S + N++ +
Sbjct: 155 LEQKLKSLEELSEKVSELKSALIVAEEEGKKSSIQMQEYQEKVSKLESSLNQSSARNSEL 214
Query: 625 EEDLPVHPHQAADH 666
EEDL + + A+H
Sbjct: 215 EEDLRIALQKGAEH 228
>At2g18540.1 68415.m02160 cupin family protein contains Pfam profile
PF00190: Cupin
Length = 707
Score = 31.9 bits (69), Expect = 0.51
Identities = 20/74 (27%), Positives = 37/74 (50%), Gaps = 1/74 (1%)
Frame = +1
Query: 451 EKRQRLEE-AEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLERGE 627
E+R++ EE A K+ +A + ++A + T +KK E + ER + +E+ +R E
Sbjct: 444 ERRRKEEEEARKREEAKRREEEEAKRREEEETERKKREEEEARKREEERKREEEEAKRRE 503
Query: 628 EDLPVHPHQAADHR 669
E+ +A R
Sbjct: 504 EERKKREEEAEQAR 517
>At1g08730.1 68414.m00969 myosin heavy chain (PCR43) identical to
myosin heavy chain PCR43 (PIR:T00727) [Arabidopsis
thaliana]; similar to ESTs gb|R30087 and gb|AA394762
Length = 1538
Score = 31.9 bits (69), Expect = 0.51
Identities = 22/91 (24%), Positives = 44/91 (48%), Gaps = 6/91 (6%)
Frame = +1
Query: 445 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKK---SENFGLSNAQLERNKTKEQL 615
+E+K+++LEE EKK Q + +++ + N + K + ++ + +++ L
Sbjct: 1018 LEDKKKKLEETEKKGQQLQESLTRMEEKCSNLESENKVLRQQAVSMAPNKFLSGRSRSIL 1077
Query: 616 ERGEED--LPVHPHQAAD-HRGSLRRQTPTE 699
+RG E L V D H S+ + P+E
Sbjct: 1078 QRGSESGHLAVDARSNLDLHSHSINHRDPSE 1108
>At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / heat
shock protein 83 (HSP83) nearly identical to SP|P27323
Heat shock protein 81-1 (HSP81-1) (Heat shock protein
83) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 705
Score = 29.5 bits (63), Expect = 2.7
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = +1
Query: 184 PKQEGEGDPEFIKRQDQKRSDLDEQLKEYINEW 282
PK+E EG+ E + + +K +++KE +EW
Sbjct: 232 PKKENEGEVEEVDEEKEKDGKKKKKIKEVSHEW 264
>At5g25060.1 68418.m02970 RNA recognition motif (RRM)-containing
protein KIAA0332 - Homo sapiens, EMBL:AB002330
Length = 946
Score = 29.5 bits (63), Expect = 2.7
Identities = 24/91 (26%), Positives = 37/91 (40%), Gaps = 4/91 (4%)
Frame = +1
Query: 451 EKRQRLEEA--EKKRQAMLQAMKDASKTGPNFTIQKK--SENFGLSNAQLERNKTKEQLE 618
+KR+R+E A E + Q MK+ + I +K ++GLS K +E
Sbjct: 797 QKRRRIEVALIEYRETLEEQGMKNPEEIERKVEINRKRLEVDYGLSGPNEGNRNQKSIIE 856
Query: 619 RGEEDLPVHPHQAADHRGSLRRQTPTEGPGT 711
R E+ HRG + Q+P T
Sbjct: 857 RKEKREDSQESSKKRHRGENKSQSPPRKSST 887
>At2g39300.1 68415.m04825 expressed protein ; expression supported
by MPSS
Length = 768
Score = 29.5 bits (63), Expect = 2.7
Identities = 14/63 (22%), Positives = 33/63 (52%)
Frame = +2
Query: 617 KEEKKISLSIRIKPLTIEGLSVDKLRQKAQELWGVHRQTRDREYDLEERQKKQDYDLKNS 796
K+E + I ++ E L++ K+ ++ +E+W ++ R R DLE ++ ++
Sbjct: 669 KDENINRMEINLQEAAKELLTLPKVLEEREEMWKEVKECRKRNMDLESEKEMLKKKVEKL 728
Query: 797 KKD 805
++D
Sbjct: 729 EED 731
>At5g41540.1 68418.m05048 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1038
Score = 28.7 bits (61), Expect = 4.8
Identities = 19/50 (38%), Positives = 24/50 (48%)
Frame = +1
Query: 442 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLE 591
D EKRQ L EAE+ R + S G +F + K SE F +S E
Sbjct: 502 DEPEKRQFLVEAEEIRDVLANETGTGSVLGISFDMSKVSE-FSISGRAFE 550
>At5g14390.1 68418.m01681 expressed protein
Length = 369
Score = 28.7 bits (61), Expect = 4.8
Identities = 20/77 (25%), Positives = 35/77 (45%), Gaps = 1/77 (1%)
Frame = +1
Query: 442 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQL-E 618
D+E RQ ++ EK RQ++ + K+ GP+ KKS+ L+R + E
Sbjct: 280 DLEMPRQSVDRREKPRQSVDKREKEKPPKGPS----KKSKLRITFEQHLDRTRRSVDFHE 335
Query: 619 RGEEDLPVHPHQAADHR 669
+ + + H H H+
Sbjct: 336 KARKSVDHHQHHQNHHQ 352
>At3g43160.1 68416.m04554 expressed protein merozoite surface
protein 2 (MSP-2), EMBL:PFU72951, Plasmodium falciparum
Length = 295
Score = 27.9 bits (59), Expect = 8.3
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = +2
Query: 215 SSSVKTRSGRTWMNS*RNTSTNGANSGPRRRMSSNALKRSR 337
+S+ +TRSGR +++ +ST NS R SS K R
Sbjct: 103 NSTTRTRSGRGFIDYVLESSTGTENSSTRTAESSTRTKNGR 143
>At2g33990.1 68415.m04162 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 249
Score = 27.9 bits (59), Expect = 8.3
Identities = 17/58 (29%), Positives = 28/58 (48%)
Frame = +2
Query: 533 PTSPSKRRAKTSV*AMPSWSATRPRSSWKEEKKISLSIRIKPLTIEGLSVDKLRQKAQ 706
P SK++ + V W+ATR ++++K K R+K + LS +K K Q
Sbjct: 30 PKKGSKKKGTSLVTRSEDWAATRIQTAFKAYKARKSLRRLKGIARAKLSTEKQSVKNQ 87
>At2g25350.1 68415.m03032 phox (PX) domain-containing protein weak
similarity to SP|Q9UTK5 Abnormal long morphology protein
1 (Sp8) {Schizosaccharomyces pombe}; contains Pfam
profile PF00787: PX domain
Length = 643
Score = 27.9 bits (59), Expect = 8.3
Identities = 22/59 (37%), Positives = 29/59 (49%)
Frame = +1
Query: 442 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLE 618
D+EE RQ+ E E K ++ D SKT N TI + E E + TK+QLE
Sbjct: 455 DMEELRQKTFEMELKLKSKEDGSSD-SKTSGNSTISESHE------LLQEMDATKQQLE 506
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.314 0.130 0.369
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,315,549
Number of Sequences: 28952
Number of extensions: 191945
Number of successful extensions: 632
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 609
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 630
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1824072800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
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