Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30805
(789 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|ch... 215 6e-57
SPBC25B2.07c |mug164||microtubule-associated protein|Schizosacch... 29 0.57
SPBC19G7.06 |mbx1||MADS-box transcription factor Mbx1|Schizosacc... 28 1.3
SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces... 27 2.3
SPAC25G10.04c |rec10|rec20|meiotic recombination protein Rec10 |... 27 3.1
SPAC18G6.02c |chp1||chromodomain protein Chp1|Schizosaccharomyce... 27 3.1
SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated prote... 27 4.0
SPAC17G8.12 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 27 4.0
SPCC285.13c |||nucleoporin Nup60 |Schizosaccharomyces pombe|chr ... 27 4.0
SPBC8D2.15 |||mitochondrial lipoic acid synthetase |Schizosaccha... 27 4.0
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 26 5.4
SPBC1A4.07c |||U3 snoRNP-associated protein Sof1|Schizosaccharom... 26 5.4
SPAC16C9.02c |||S-methyl-5-thioadenosine phosphorylase|Schizosac... 26 7.1
SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 26 7.1
>SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 509
Score = 215 bits (525), Expect = 6e-57
Identities = 109/205 (53%), Positives = 135/205 (65%), Gaps = 1/205 (0%)
Frame = +2
Query: 158 RLSGIICTIGPASRNVAVLEKMMETGMNVARMNFSHGSHEYHAETIRNCREAEKSYSAKL 337
R + IICTIGP S NV L K+ + GMN+ RMNFSHGS+EYH I N R+A +
Sbjct: 26 RRTSIICTIGPKSNNVETLCKLRDAGMNIVRMNFSHGSYEYHQSVIDNARKASATNPL-- 83
Query: 338 GSPFSLAIALDTKGPEIRTGLLEGGGSAEVELKKGETIKLTTSSDYQEKGNADTIYVDYK 517
F LAIALDTKGPEIRTGL GG + + G + TT Y EK N +Y+DYK
Sbjct: 84 ---FPLAIALDTKGPEIRTGLTVGG--TDYPISSGHEMIFTTDDAYAEKCNDKVMYIDYK 138
Query: 518 NITNVVKPGNRIFIDDGLISI-ICQSVSADTLTCTIENGGMLGSRKGVNLPGIPVDLPAV 694
NIT V++PG I++DDG++S + + V L + N G + S+KGVNLP VDLPA+
Sbjct: 139 NITKVIQPGRIIYVDDGILSFTVIEKVDDKNLKVRVNNNGKISSKKGVNLPKTDVDLPAL 198
Query: 695 SEKDKSDLLFGVEQGVDMIFASFIR 769
SEKDK+DL FGV+ GVDMIFASFIR
Sbjct: 199 SEKDKADLRFGVKNGVDMIFASFIR 223
>SPBC25B2.07c |mug164||microtubule-associated
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 501
Score = 29.5 bits (63), Expect = 0.57
Identities = 49/206 (23%), Positives = 81/206 (39%), Gaps = 3/206 (1%)
Frame = +2
Query: 41 TIHDGEIEAGAVEKPTVANVGSQLQHMCGLDIDSKSSYIRLSGI--ICTIGPASRNVAVL 214
++H+ I + E P+V +H+ + S SS R+S I I A ++AV
Sbjct: 130 SLHENSISSK--ESPSVTTS----KHVATITKPSTSSIARMSSNARIAAIPRAKSSMAVR 183
Query: 215 E-KMMETGMNVARMNFSHGSHEYHAETIRNCREAEKSYSAKLGSPFSLAIALDTKGPEIR 391
+ G NV + + +++ +K ++ S SL + + P R
Sbjct: 184 SPSRLGNGPNVRSPKVGFNAKSDDSPVVKSPGSNDKPSASPRISVRSLGNSSVVR-PPTR 242
Query: 392 TGLLEGGGSAEVELKKGETIKLTTSSDYQEKGNADTIYVDYKNITNVVKPGNRIFIDDGL 571
T V G K +TS + K NA T V + V+PG+RIF ++
Sbjct: 243 TSTTRPLSRVNVTNASGSISKNSTSPS-KVKVNASTKIVRPVSAAQTVRPGSRIFRENSA 301
Query: 572 ISIICQSVSADTLTCTIENGGMLGSR 649
+ +VSA T T+ L R
Sbjct: 302 SNTQRPNVSA-TSNSTVRVASSLAVR 326
>SPBC19G7.06 |mbx1||MADS-box transcription factor
Mbx1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 436
Score = 28.3 bits (60), Expect = 1.3
Identities = 13/23 (56%), Positives = 15/23 (65%)
Frame = +3
Query: 675 PWTYPQSPRRTSRTCSSASNKAL 743
P T P SPRR+ + S A NKAL
Sbjct: 7 PSTAPSSPRRSIQRISDAKNKAL 29
>SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 713
Score = 27.5 bits (58), Expect = 2.3
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = -3
Query: 622 NGTRKSVSADRLTDDRDEAIIDEDSVSWLHDIR 524
N TRKS+S + TD + +D SWL +R
Sbjct: 576 NKTRKSISHSKNTDKHVGNELPQDIPSWLRSLR 608
>SPAC25G10.04c |rec10|rec20|meiotic recombination protein Rec10
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 791
Score = 27.1 bits (57), Expect = 3.1
Identities = 11/40 (27%), Positives = 22/40 (55%)
Frame = +2
Query: 68 GAVEKPTVANVGSQLQHMCGLDIDSKSSYIRLSGIICTIG 187
G + + ++ L+ + D+D+K +YI+LS T+G
Sbjct: 15 GTLNESSIYKTYQILESLNPKDVDTKENYIKLSNTFSTLG 54
>SPAC18G6.02c |chp1||chromodomain protein Chp1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 960
Score = 27.1 bits (57), Expect = 3.1
Identities = 27/94 (28%), Positives = 40/94 (42%)
Frame = -3
Query: 643 SEHTSVFNGTRKSVSADRLTDDRDEAIIDEDSVSWLHDIRYVLVVHVNCISIAFLLIIRT 464
S+ T +G K V A +T + D ++ S+S D+ + +S F ++R
Sbjct: 474 SDQTFENDGASKVVPAGNITLNSDNSL--HHSISESEDLS-----SASTLSDYFRFVLRV 526
Query: 463 CRQLYSLSLLQFDFS*AAAFQEPSSDLRSFSVQC 362
+ LY L FD S A E LRS V C
Sbjct: 527 GKSLYYAGELSFDISKLKAETEHQQLLRSL-VSC 559
>SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated protein
Mug36|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1646
Score = 26.6 bits (56), Expect = 4.0
Identities = 25/119 (21%), Positives = 53/119 (44%), Gaps = 1/119 (0%)
Frame = +2
Query: 23 LKMVYPTIH-DGEIEAGAVEKPTVANVGSQLQHMCGLDIDSKSSYIRLSGIICTIGPASR 199
LK+ + I+ + ++ G + T A + S ++ +C DS S ++S P ++
Sbjct: 1151 LKVKFKDINVNWDLHDGYDWEATRATISSAIEKLC----DSSSQNDKIS-------PEAK 1199
Query: 200 NVAVLEKMMETGMNVARMNFSHGSHEYHAETIRNCREAEKSYSAKLGSPFSLAIALDTK 376
+ ++++ V R+N +H S ++ + SY +LG S I ++ K
Sbjct: 1200 TLLFQSIVIKSFSKVGRLNINHVSEPIDSDEFADYLSKSISYHLRLGKSKSKKIGIEIK 1258
>SPAC17G8.12 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 608
Score = 26.6 bits (56), Expect = 4.0
Identities = 23/89 (25%), Positives = 36/89 (40%), Gaps = 4/89 (4%)
Frame = -1
Query: 414 PP--PSKSPVLISGPLVSNAMAKEKGEPSLAL*LFSASRQLRMVSAWYSWEPCEKFILAT 241
PP PS+ P + + P + EPS+ L LR + + +P K L T
Sbjct: 430 PPKHPSRQPTVRATPSTGKQIEPPNDEPSIGNELLPLFESLRFENRKTTSKPKAKKTLVT 489
Query: 240 FIPVSIIFSSTATLRDAGPIVQI--IPER 160
IP +S + D + + IPE+
Sbjct: 490 SIPTKPHQASVEKVYDLNSKINLKNIPEQ 518
>SPCC285.13c |||nucleoporin Nup60 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 736
Score = 26.6 bits (56), Expect = 4.0
Identities = 15/38 (39%), Positives = 21/38 (55%)
Frame = -1
Query: 444 SPFFSSTSAEPPPSKSPVLISGPLVSNAMAKEKGEPSL 331
SP ST+A P K+P + L+S +KEK PS+
Sbjct: 305 SPLHQSTTANHP-EKTPSRAAASLLSILDSKEKNTPSI 341
>SPBC8D2.15 |||mitochondrial lipoic acid synthetase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 370
Score = 26.6 bits (56), Expect = 4.0
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = +1
Query: 496 YNLRGLQEHNECREARKPNLHR*W 567
++LRG H C EA+ PN+ W
Sbjct: 89 HDLRGSHLHTVCEEAKCPNIGECW 112
>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 4717
Score = 26.2 bits (55), Expect = 5.4
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +1
Query: 130 RH*LQVFVHSSLGNNLYYWTGIP*CSCAR 216
RH L++ V L N+Y WT +P C +R
Sbjct: 2344 RHFLKLVV---LWKNIYVWTNVPDCDISR 2369
>SPBC1A4.07c |||U3 snoRNP-associated protein
Sof1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 436
Score = 26.2 bits (55), Expect = 5.4
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = +1
Query: 625 KRRYARIPERRQPARHTRGPTRSLREGQV 711
+ RY IPE R+ ARH PT + ++
Sbjct: 369 RERYKHIPEIRRIARHRHLPTNVKKAAEI 397
>SPAC16C9.02c |||S-methyl-5-thioadenosine
phosphorylase|Schizosaccharomyces pombe|chr 1|||Manual
Length = 307
Score = 25.8 bits (54), Expect = 7.1
Identities = 11/22 (50%), Positives = 12/22 (54%)
Frame = +3
Query: 657 STCPAYPWTYPQSPRRTSRTCS 722
S P PW YP SP +RT S
Sbjct: 29 SVNPITPWGYPASPISIARTTS 50
>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 513
Score = 25.8 bits (54), Expect = 7.1
Identities = 10/17 (58%), Positives = 11/17 (64%)
Frame = -1
Query: 444 SPFFSSTSAEPPPSKSP 394
SP FSS+S PPP P
Sbjct: 231 SPIFSSSSTPPPPPPRP 247
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,175,398
Number of Sequences: 5004
Number of extensions: 65560
Number of successful extensions: 230
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 220
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 227
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 383374054
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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