Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30800
(765 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g74510.2 68414.m08632 kelch repeat-containing F-box family pr... 30 1.9
At1g74510.1 68414.m08631 kelch repeat-containing F-box family pr... 30 1.9
At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest ... 29 3.4
At5g28210.1 68418.m03416 mRNA capping enzyme family protein simi... 29 4.5
At4g39980.1 68417.m05662 2-dehydro-3-deoxyphosphoheptonate aldol... 29 4.5
At1g72110.1 68414.m08335 expressed protein 29 4.5
At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5) M... 28 5.9
At1g05790.1 68414.m00605 lipase class 3 family protein contains ... 28 5.9
At1g80870.1 68414.m09489 protein kinase family protein contains ... 28 7.8
>At1g74510.2 68414.m08632 kelch repeat-containing F-box family
protein contains Pfam:PF01344 Kelch motif, Pfam:PF00646
F-box domain
Length = 451
Score = 29.9 bits (64), Expect = 1.9
Identities = 20/59 (33%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
Frame = +2
Query: 221 KVSEGYGAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNLGGNPP--AVPVVAQG 391
K S G E + FG EI Y Y TN + + VPR L G+ + V+A G
Sbjct: 185 KESLAVGTELLVFGKEIMSHVIYRYSILTNTWTSGMQMNVPRCLFGSASLGEIAVIAGG 243
>At1g74510.1 68414.m08631 kelch repeat-containing F-box family
protein contains Pfam:PF01344 Kelch motif, Pfam:PF00646
F-box domain
Length = 451
Score = 29.9 bits (64), Expect = 1.9
Identities = 20/59 (33%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
Frame = +2
Query: 221 KVSEGYGAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNLGGNPP--AVPVVAQG 391
K S G E + FG EI Y Y TN + + VPR L G+ + V+A G
Sbjct: 185 KESLAVGTELLVFGKEIMSHVIYRYSILTNTWTSGMQMNVPRCLFGSASLGEIAVIAGG 243
>At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest
subunit (RPB205) (RPII) (RPB1) nearly identical to
P|P18616 DNA-directed RNA polymerase II largest subunit
(EC 2.7.7.6) {Arabidopsis thaliana}
Length = 1840
Score = 29.1 bits (62), Expect = 3.4
Identities = 21/66 (31%), Positives = 27/66 (40%), Gaps = 3/66 (4%)
Frame = +2
Query: 305 TNNGIAAQEQGVPRNLGGNPPAVPVVAQGS--FSWTSPEGVPISVNYVADENGYQPTGNA 478
T+ G + G G P P + S +S TSP P S +Y Y PT +
Sbjct: 1555 TSPGYSPTSPGYSPTSPGYSPTSPTYSPSSPGYSPTSPAYSPTSPSYSPTSPSYSPTSPS 1614
Query: 479 I-PTSP 493
PTSP
Sbjct: 1615 YSPTSP 1620
Score = 29.1 bits (62), Expect = 3.4
Identities = 21/57 (36%), Positives = 26/57 (45%), Gaps = 4/57 (7%)
Frame = +2
Query: 392 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSPPVPEQIAR---ALAYIAKNIPL 550
S+S TSP P S Y Y PT + PTSP Q A+ ++AY N L
Sbjct: 1691 SYSPTSPAYSPTSPGYSPTSPSYSPTSPSYGPTSPSYNPQSAKYSPSIAYSPSNARL 1747
Score = 28.7 bits (61), Expect = 4.5
Identities = 22/72 (30%), Positives = 27/72 (37%), Gaps = 1/72 (1%)
Frame = +2
Query: 281 GSYTYFYETNNGIAAQEQGVPRNLGGNPPAVPVVAQGSFSWTSPEGVPISVNYVADENGY 460
G + ++ G + G G P P +S TSP P S Y GY
Sbjct: 1533 GGMAFSPSSSPGYSPSSPGYSPTSPGYSPTSP-----GYSPTSPGYSPTSPTYSPSSPGY 1587
Query: 461 QPTGNAI-PTSP 493
PT A PTSP
Sbjct: 1588 SPTSPAYSPTSP 1599
Score = 28.3 bits (60), Expect = 5.9
Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 392 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 493
S+S TSP P S +Y Y PT A PTSP
Sbjct: 1614 SYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSP 1648
Score = 28.3 bits (60), Expect = 5.9
Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 392 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 493
S+S TSP P S +Y Y PT A PTSP
Sbjct: 1621 SYSPTSPSYSPTSPSYSPTSPAYSPTSPAYSPTSP 1655
Score = 28.3 bits (60), Expect = 5.9
Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 392 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 493
S+S TSP P S +Y Y PT A PTSP
Sbjct: 1670 SYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSP 1704
Score = 28.3 bits (60), Expect = 5.9
Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 392 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 493
S+S TSP P S Y GY PT + PTSP
Sbjct: 1684 SYSPTSPSYSPTSPAYSPTSPGYSPTSPSYSPTSP 1718
>At5g28210.1 68418.m03416 mRNA capping enzyme family protein similar
to mRNA capping enzyme [Xenopus laevis] GI:7239232;
contains Pfam profiles PF01331: mRNA capping enzyme
catalytic domain, PF03919: mRNA capping enzyme
C-terminal domain
Length = 625
Score = 28.7 bits (61), Expect = 4.5
Identities = 12/34 (35%), Positives = 21/34 (61%)
Frame = -2
Query: 422 EHLQEMSRRMSPVQRREQQGGFHQGYVELPVLGQ 321
E+ +E S+R+ P R+Q+ QG+++ P GQ
Sbjct: 27 ENREEHSKRLRPTSFRDQKVTIPQGWLDCPRFGQ 60
>At4g39980.1 68417.m05662 2-dehydro-3-deoxyphosphoheptonate aldolase
1 / 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
1 / DAHP synthetase 1 (DHS1) nearly identical to
SP|P29965
Length = 525
Score = 28.7 bits (61), Expect = 4.5
Identities = 23/76 (30%), Positives = 34/76 (44%), Gaps = 1/76 (1%)
Frame = +2
Query: 353 GGNPPAVPVVAQGSFSWTSPEGV-PISVNYVADENGYQPTGNAIPTSPPVPEQIARALAY 529
GG+ P Q SF++ P SVN V + +P NA+ V + AL +
Sbjct: 16 GGDLSHRPSNRQSSFTFHPAVNTKPKSVNLVTAVHAAEPARNAVSVKESVASSSSGALKW 75
Query: 530 IAKNIPLKK*AINLTD 577
++ LKK A+ L D
Sbjct: 76 TPESWKLKK-ALQLPD 90
>At1g72110.1 68414.m08335 expressed protein
Length = 479
Score = 28.7 bits (61), Expect = 4.5
Identities = 14/39 (35%), Positives = 21/39 (53%)
Frame = +2
Query: 236 YGAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNL 352
+G+ T+ F N + PD ++F IAA GVP+ L
Sbjct: 388 FGSTTMIFSNVVGPDEEISFFGHRIAYIAASTFGVPQAL 426
>At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5)
Monovalent cation:proton antiporter family 2 (CPA2
family) member, PMID:11500563; related to
glutathione-regulated potassium-efflux system protein
[Escherichia coli] GP|606284|gb|AAA58147
Length = 568
Score = 28.3 bits (60), Expect = 5.9
Identities = 16/37 (43%), Positives = 24/37 (64%)
Frame = +3
Query: 345 VTLVETPLLFPSLHRAHSPGHLLKVFPSASITSPTRT 455
++LV TPLLF + A + G LL+ FPS + +SP +
Sbjct: 518 LSLVTTPLLFKLIPSAMNLGVLLRWFPSEN-SSPNES 553
>At1g05790.1 68414.m00605 lipase class 3 family protein contains
Pfam profile PF01764: Lipase
Length = 687
Score = 28.3 bits (60), Expect = 5.9
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = -3
Query: 487 SGDSITSGLVSVLVGDVIDADGNTFR 410
SG+S +SG +S L+GD + DG + R
Sbjct: 398 SGESESSGFLSSLIGDGCECDGYSIR 423
>At1g80870.1 68414.m09489 protein kinase family protein contains
eukaryotic protein kinase domain, INTERPRO:IPR000719
Length = 692
Score = 27.9 bits (59), Expect = 7.8
Identities = 16/46 (34%), Positives = 22/46 (47%), Gaps = 4/46 (8%)
Frame = -3
Query: 556 LLERNVLGDVGKSTSDLLRHWW----GSGDSITSGLVSVLVGDVID 431
LL N + D+GK + R WW GSG+ + V +G ID
Sbjct: 330 LLSPNSVLDLGKGSKQWGRDWWWKQEGSGELCSKDYVREWIGSQID 375
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,539,772
Number of Sequences: 28952
Number of extensions: 378673
Number of successful extensions: 1142
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 1076
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1128
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1712086600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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