BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30769 (779 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 33 0.061 SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1 |Schizosacch... 31 0.24 SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces pomb... 31 0.24 SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Sch... 30 0.43 SPBC713.09 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 29 0.75 SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schiz... 29 0.75 SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces pombe... 29 0.75 SPAC6G10.02c |tea3||cell end marker Tea3|Schizosaccharomyces pom... 28 1.3 SPBC25B2.05 |mis3||rRNA processing protein Mis3|Schizosaccharomy... 28 1.3 SPAC3H8.09c |nab3||poly|Schizosaccharomyces pombe|chr 1|||Manual 28 1.7 SPBC3E7.13c |||splicing factor, SYF2 family|Schizosaccharomyces ... 28 1.7 SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 27 2.3 SPAC4F8.04 |||Brix domain protein Rpf1|Schizosaccharomyces pombe... 27 2.3 SPBC1105.04c |cbp1|abp1|CENP-B homolog|Schizosaccharomyces pombe... 27 2.3 SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces... 27 3.0 SPAC4F8.02c |mrpl40|SPAC644.02|mitochondrial ribosomal protein s... 26 5.3 SPAC11E3.11c |||guanyl-nucleotide exchange factor |Schizosacchar... 26 5.3 SPAPB8E5.07c |||ribosome biogenesis protein Rrp12|Schizosaccharo... 26 5.3 SPCC594.05c |||COMPASS complex subunit |Schizosaccharomyces pomb... 26 7.0 SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein Pkl1|S... 26 7.0 SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|ch... 26 7.0 SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 25 9.2 SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 25 9.2 SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 25 9.2 >SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1085 Score = 32.7 bits (71), Expect = 0.061 Identities = 28/119 (23%), Positives = 57/119 (47%), Gaps = 5/119 (4%) Frame = +3 Query: 363 KRVCKDYHERIARLEDEKF--DLEYIVKRK-DMEISDLNSQVNDLRGKFVKPTLKKVSKY 533 +RV + Y E++A+ K ++E KRK + E+ + + K K+ + Sbjct: 554 QRVLQAYREKVAQQRQAKLLEEIEEENKRKQERELKKIREKEKKRDKKKQLKLAKEEERQ 613 Query: 534 ENKFAKLQKKAAE--FNFRNQLKVVKKKEFTLEEEDKEKKPDWSKGKPGDQKVKEKEVE 704 + +L ++AA+ + Q + KK+E + ++EKK + + ++K K+KE E Sbjct: 614 RREAERLAEQAAQKALEAKRQEEARKKREEQRLKREQEKKQQELERQKREEKQKQKERE 672 >SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1327 Score = 30.7 bits (66), Expect = 0.24 Identities = 15/65 (23%), Positives = 31/65 (47%) Frame = +3 Query: 507 PTLKKVSKYENKFAKLQKKAAEFNFRNQLKVVKKKEFTLEEEDKEKKPDWSKGKPGDQKV 686 PT KK + ++ K + ++ +E N + VK + L+ ++EK P W + + Sbjct: 531 PTAKKNNSFKPKLFRRSRELSEKLCNNWSERVKSPSYQLKVREREKLPAWESRRKIMDAI 590 Query: 687 KEKEV 701 + +V Sbjct: 591 QHSQV 595 >SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces pombe|chr 2|||Manual Length = 814 Score = 30.7 bits (66), Expect = 0.24 Identities = 19/59 (32%), Positives = 29/59 (49%), Gaps = 2/59 (3%) Frame = +3 Query: 534 ENKFAKLQKKAAEFNFRNQLKVVKKKEFTLEEEDKEKKPDWSKGKPGDQK--VKEKEVE 704 +N F K E NF + +K K +FT E+K + K P +QK +K+K+ E Sbjct: 254 DNFFRDFLKVCDECNFNHNIKEFSKCDFTQMFHHFEQKREEKKSMPKEQKKAIKQKKDE 312 >SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1194 Score = 29.9 bits (64), Expect = 0.43 Identities = 14/56 (25%), Positives = 29/56 (51%) Frame = +3 Query: 321 GKPKNIDDANEDTIKRVCKDYHERIARLEDEKFDLEYIVKRKDMEISDLNSQVNDL 488 G ID++ +++KR Y ++ ++ LE ++R + EISD +++ L Sbjct: 837 GSDNRIDESELNSVKRSLLKYENKLQIIKSSSSGLEEQMQRINSEISDKRNELESL 892 >SPBC713.09 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 395 Score = 29.1 bits (62), Expect = 0.75 Identities = 29/103 (28%), Positives = 49/103 (47%), Gaps = 2/103 (1%) Frame = +3 Query: 402 LEDEKFDLEYIVKRKDMEISDLNSQVNDLRGKFVKPTLK-KVSKYENKFAKLQKK-AAEF 575 LE + L + ++KD +I +L ++NDL + TLK E K + K+ AA Sbjct: 149 LEKQVKTLHDLNEQKDKKIKELKERINDLTYDY--ETLKANADDSEGKQTLVSKREAALE 206 Query: 576 NFRNQLKVVKKKEFTLEEEDKEKKPDWSKGKPGDQKVKEKEVE 704 F+++L + E + K+ GK D K +EK++E Sbjct: 207 EFQSKL--------LIRENEINKRELKMNGKEDDLKKREKDLE 241 >SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1253 Score = 29.1 bits (62), Expect = 0.75 Identities = 13/35 (37%), Positives = 19/35 (54%) Frame = +3 Query: 576 NFRNQLKVVKKKEFTLEEEDKEKKPDWSKGKPGDQ 680 +FRNQ+K ++ L E+ KE W +G DQ Sbjct: 1070 DFRNQIKSQTERILVLREKKKEDPISWMEGTMEDQ 1104 >SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 972 Score = 29.1 bits (62), Expect = 0.75 Identities = 19/68 (27%), Positives = 34/68 (50%), Gaps = 3/68 (4%) Frame = +3 Query: 381 YHERIARLEDEKFDLEYIVKRKDMEISDLNS---QVNDLRGKFVKPTLKKVSKYENKFAK 551 +H+ + +DE Y++ K ME +L+ QVN + K +K VS + N +A+ Sbjct: 578 FHQLVFSADDEYLASVYLIYLKQMETKNLSEEKPQVNKIVKKILKKYDSSVSVW-NTYAQ 636 Query: 552 LQKKAAEF 575 L+ + F Sbjct: 637 LEHLSGAF 644 >SPAC6G10.02c |tea3||cell end marker Tea3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1125 Score = 28.3 bits (60), Expect = 1.3 Identities = 16/58 (27%), Positives = 32/58 (55%) Frame = +3 Query: 363 KRVCKDYHERIARLEDEKFDLEYIVKRKDMEISDLNSQVNDLRGKFVKPTLKKVSKYE 536 +R+C+ + + + + +DL++ E S L+ QVNDL + K T +K+S+ + Sbjct: 897 QRLCEGRTKELLNSQQKLYDLKHSYSSVMTEKSKLSDQVNDLTEQ-AKITQRKLSEVQ 953 >SPBC25B2.05 |mis3||rRNA processing protein Mis3|Schizosaccharomyces pombe|chr 2|||Manual Length = 327 Score = 28.3 bits (60), Expect = 1.3 Identities = 13/35 (37%), Positives = 22/35 (62%) Frame = +3 Query: 600 VKKKEFTLEEEDKEKKPDWSKGKPGDQKVKEKEVE 704 ++ E+ L++E+KE+K K + +K KEKE E Sbjct: 275 IESGEYFLKKEEKERKKRAEKKEQQKEKKKEKEKE 309 >SPAC3H8.09c |nab3||poly|Schizosaccharomyces pombe|chr 1|||Manual Length = 738 Score = 27.9 bits (59), Expect = 1.7 Identities = 16/71 (22%), Positives = 33/71 (46%), Gaps = 1/71 (1%) Frame = +3 Query: 474 QVNDLRGKFVKPTLKKVSK-YENKFAKLQKKAAEFNFRNQLKVVKKKEFTLEEEDKEKKP 650 Q N +RG+ + + K+ K Y+N+ ++K + +++ + + + P Sbjct: 418 QGNFVRGQKLHLEISKIQKKYQNQIENMKKGSHVTKSNQYSEMIGNLPYPTSSRKRTRSP 477 Query: 651 DWSKGKPGDQK 683 SKGK D+K Sbjct: 478 LMSKGKSYDRK 488 >SPBC3E7.13c |||splicing factor, SYF2 family|Schizosaccharomyces pombe|chr 2|||Manual Length = 229 Score = 27.9 bits (59), Expect = 1.7 Identities = 33/133 (24%), Positives = 63/133 (47%), Gaps = 9/133 (6%) Frame = +3 Query: 336 IDDANEDTIKRVCKDYHERIARLEDEKFDLEYIVKRK-DMEISDLNSQVNDLRGKFVKPT 512 ID A E ++R + E +A++E E+ ++ +R D I + LR K K T Sbjct: 42 IDPALERRLERKRMEAEEELAKIETEEEGGDFERRRAWDWTIEESEKWDQRLRKK--KQT 99 Query: 513 LKKV--SKYENKFAK-----LQKKAAEFN-FRNQLKVVKKKEFTLEEEDKEKKPDWSKGK 668 ++ V S Y ++ +K ++ ++ + K K K ++ D+ ++ DW K Sbjct: 100 IQNVAFSDYHSQASKDYNRGIRDLKPDYEKYDKDKKEEKSKSRSVALYDEAERLDWVSNK 159 Query: 669 PGDQKVKEKEVEA 707 P D++ E+ VE+ Sbjct: 160 P-DKEHVERLVES 171 >SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 2363 Score = 27.5 bits (58), Expect = 2.3 Identities = 20/88 (22%), Positives = 42/88 (47%), Gaps = 1/88 (1%) Frame = +3 Query: 438 KRKDMEISDLNSQVNDLRGKFVKPTLKK-VSKYENKFAKLQKKAAEFNFRNQLKVVKKKE 614 K+ D I+ S + G+ + P L + + +E++F Q+ AE+ + Q + + + Sbjct: 1368 KQTDTGITHFRSGMTT-NGEHLIPNLYRYIQPWESEFIDSQRVWAEYAMKRQEALQQNRR 1426 Query: 615 FTLEEEDKEKKPDWSKGKPGDQKVKEKE 698 TLE + + W +G P + +K+ Sbjct: 1427 LTLE----DLEDSWDRGIPRINTLFQKD 1450 >SPAC4F8.04 |||Brix domain protein Rpf1|Schizosaccharomyces pombe|chr 1|||Manual Length = 306 Score = 27.5 bits (58), Expect = 2.3 Identities = 15/43 (34%), Positives = 25/43 (58%), Gaps = 1/43 (2%) Frame = +3 Query: 522 VSKYENKFAKLQKKAAEFNFRNQLKVVKKKEFTLEEE-DKEKK 647 + K +NK + Q+ + +N+ K+ ++KE EEE D EKK Sbjct: 1 MGKIKNKIVRQQQYMKALHQKNKDKLERRKERAKEEEKDPEKK 43 >SPBC1105.04c |cbp1|abp1|CENP-B homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 522 Score = 27.5 bits (58), Expect = 2.3 Identities = 13/40 (32%), Positives = 22/40 (55%) Frame = +3 Query: 330 KNIDDANEDTIKRVCKDYHERIARLEDEKFDLEYIVKRKD 449 +NI D++ED I ++ Y + A DE+ E+ + KD Sbjct: 435 ENIVDSSEDIINQIASQYMDDRAFETDEEESTEFQITTKD 474 >SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces pombe|chr 3|||Manual Length = 1526 Score = 27.1 bits (57), Expect = 3.0 Identities = 21/85 (24%), Positives = 44/85 (51%), Gaps = 1/85 (1%) Frame = +3 Query: 441 RKDMEISDLNSQVNDLRGKFVKPTLKKVS-KYENKFAKLQKKAAEFNFRNQLKVVKKKEF 617 R ++E +L QVN+L+G V P S ++ N ++ KA ++Q+ K K F Sbjct: 1423 RLNLEKHNLEKQVNELKGAEVSPQPTGQSLQHVNLAHAIELKA----LKDQINSEKAKMF 1478 Query: 618 TLEEEDKEKKPDWSKGKPGDQKVKE 692 +++ + ++++ + K +KV + Sbjct: 1479 SVQVQYEKREQELQKRIASLEKVNK 1503 >SPAC4F8.02c |mrpl40|SPAC644.02|mitochondrial ribosomal protein subunit L40|Schizosaccharomyces pombe|chr 1|||Manual Length = 279 Score = 26.2 bits (55), Expect = 5.3 Identities = 12/41 (29%), Positives = 20/41 (48%) Frame = +3 Query: 558 KKAAEFNFRNQLKVVKKKEFTLEEEDKEKKPDWSKGKPGDQ 680 KK + R +++ K ++DK KP+ GKP D+ Sbjct: 22 KKPLPLHMRTKIREHLNKSDPTVKDDKSAKPELPFGKPADR 62 >SPAC11E3.11c |||guanyl-nucleotide exchange factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 942 Score = 26.2 bits (55), Expect = 5.3 Identities = 16/32 (50%), Positives = 19/32 (59%) Frame = -3 Query: 684 PSDLPACPSTSQAFSLCLPLPR*IPSFSRPSI 589 PS LP PSTS +F +PL PSFS +I Sbjct: 150 PSQLPVRPSTSLSFFDDIPLG---PSFSAETI 178 >SPAPB8E5.07c |||ribosome biogenesis protein Rrp12|Schizosaccharomyces pombe|chr 1|||Manual Length = 1163 Score = 26.2 bits (55), Expect = 5.3 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = -2 Query: 199 KPFFAFLALEASSMRLRTSARLRSISACL 113 K FF L E+ +RL+T+ + S+ CL Sbjct: 325 KAFFTLLESESMEIRLQTATTICSVIGCL 353 >SPCC594.05c |||COMPASS complex subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 424 Score = 25.8 bits (54), Expect = 7.0 Identities = 13/44 (29%), Positives = 22/44 (50%) Frame = +3 Query: 351 EDTIKRVCKDYHERIARLEDEKFDLEYIVKRKDMEISDLNSQVN 482 ED+ + D+H A E+EK + K ME+ ++N V+ Sbjct: 23 EDSNRGTITDFHIETANNEEEKDANVILNKSVKMEVEEVNGHVD 66 >SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein Pkl1|Schizosaccharomyces pombe|chr 1|||Manual Length = 832 Score = 25.8 bits (54), Expect = 7.0 Identities = 12/41 (29%), Positives = 22/41 (53%) Frame = -1 Query: 428 FQIEFFIFKTSDAFMVVFANPLNSIFVGVVNVLRFTAPLLD 306 + +++ + K + M V +PL S F+ +N LRF + D Sbjct: 781 YLLKYSLGKGAKTLMFVNVSPLKSQFMDTLNSLRFATKVND 821 >SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1208 Score = 25.8 bits (54), Expect = 7.0 Identities = 11/33 (33%), Positives = 21/33 (63%) Frame = +3 Query: 381 YHERIARLEDEKFDLEYIVKRKDMEISDLNSQV 479 +HE I +L+D + +L +++K++ IS L V Sbjct: 599 FHESINKLQDREKELTSNLEKKNLVISSLRETV 631 >SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1049 Score = 25.4 bits (53), Expect = 9.2 Identities = 11/43 (25%), Positives = 19/43 (44%) Frame = -3 Query: 768 TSHNY*QLVKGLNDLGIMMFMPQPLFLLPSDLPACPSTSQAFS 640 TS NY +K + L + F+P+ + + C +T S Sbjct: 56 TSENYDSFIKEIKTLSLKKFIPEITAAIVEGMMKCKATKDILS 98 >SPCC18.03 |||shuttle craft like transcriptional regulator|Schizosaccharomyces pombe|chr 3|||Manual Length = 1077 Score = 25.4 bits (53), Expect = 9.2 Identities = 10/24 (41%), Positives = 12/24 (50%) Frame = -3 Query: 705 PQPLFLLPSDLPACPSTSQAFSLC 634 P PL P P C +T + F LC Sbjct: 293 PCPLLCHPGPCPPCTATVEKFCLC 316 >SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces pombe|chr 1|||Manual Length = 1957 Score = 25.4 bits (53), Expect = 9.2 Identities = 19/92 (20%), Positives = 36/92 (39%) Frame = +3 Query: 360 IKRVCKDYHERIARLEDEKFDLEYIVKRKDMEISDLNSQVNDLRGKFVKPTLKKVSKYEN 539 +K + H ++ E + E K I + + ++ + K VK ++ + + Sbjct: 376 LKESNRTIHSQLTDAESKLSSFEQENKSLKGSIDEYQNNLSS-KDKMVKQVSSQLEEARS 434 Query: 540 KFAKLQKKAAEFNFRNQLKVVKKKEFTLEEED 635 A K AE N + K K+F E+D Sbjct: 435 SLAHATGKLAEINSERDFQNKKIKDFEKIEQD 466 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,418,473 Number of Sequences: 5004 Number of extensions: 42041 Number of successful extensions: 224 Number of sequences better than 10.0: 24 Number of HSP's better than 10.0 without gapping: 214 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 222 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 377352472 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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