BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30683 (722 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC126.03 |pus1|SPCC126.03, SPCC126.03|tRNA pseudouridylate syn... 36 0.006 SPBC887.11 |pus2||tRNA pseudouridylate synthase Pus2 |Schizosacc... 30 0.29 SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces po... 29 0.89 >SPCC126.03 |pus1|SPCC126.03, SPCC126.03|tRNA pseudouridylate synthase Lsp1|Schizosaccharomyces pombe|chr 3|||Manual Length = 534 Score = 35.9 bits (79), Expect = 0.006 Identities = 18/67 (26%), Positives = 34/67 (50%) Frame = +3 Query: 372 ERIKRKKMAMLLGYSGVEYYGMQRNPGVQTIXXXXXXXXXXXXYITQEDFENAQNAQFQR 551 E+ ++K+A L+GY G Y+GMQ NP +TI ++ + ++ + R Sbjct: 39 EKKPKRKVACLVGYCGSGYHGMQLNPPSKTIEGDLFDAFVKAGAVSSYNADDPKKVALAR 98 Query: 552 SSRD*QG 572 ++R +G Sbjct: 99 AARTDKG 105 >SPBC887.11 |pus2||tRNA pseudouridylate synthase Pus2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 451 Score = 30.3 bits (65), Expect = 0.29 Identities = 17/61 (27%), Positives = 32/61 (52%) Frame = +3 Query: 390 KMAMLLGYSGVEYYGMQRNPGVQTIXXXXXXXXXXXXYITQEDFENAQNAQFQRSSRD*Q 569 ++ +LLGYSG Y+G+Q N ++TI Y+ + + A++ R++R + Sbjct: 40 RIIILLGYSGYGYHGIQINNPLKTIEGDVVAVLKKLGYLKTNNID-AEHLCIARAARTDK 98 Query: 570 G 572 G Sbjct: 99 G 99 >SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces pombe|chr 3|||Manual Length = 2812 Score = 28.7 bits (61), Expect = 0.89 Identities = 11/32 (34%), Positives = 18/32 (56%) Frame = -2 Query: 445 FRCIP*YSTPEYPSSIAIFFLLILSYGFLISP 350 ++C Y E+P S+ +FF I+ + F SP Sbjct: 1520 WKCYKAYDITEFPDSLKLFFSDIMGWNFFKSP 1551 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,875,953 Number of Sequences: 5004 Number of extensions: 58716 Number of successful extensions: 172 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 167 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 172 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 339215786 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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