Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30671
(643 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 93 2e-20
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 87 2e-18
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 66 4e-12
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 54 1e-08
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 48 2e-06
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 28 1.00
SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 25 7.0
SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|c... 25 9.3
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 93.5 bits (222), Expect = 2e-20
Identities = 51/134 (38%), Positives = 78/134 (58%), Gaps = 6/134 (4%)
Frame = +1
Query: 205 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 372
+ +Q+AEF+EAF L D D+DG I N+L SLG+ + EL +M+ E +G I+
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66
Query: 373 FTQLLTLFANRMSGGSDEDDVVINAFKTFDEEGK--IDSERLRHALMTWGDKFSADEVDE 546
FT+ LT+ A +M +D ++ V AFK FD++G I E L H L + G++ S +EV +
Sbjct: 67 FTEFLTMMARKMK-DTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVAD 125
Query: 547 AYDQMDIDDKGYID 588
+ D D G I+
Sbjct: 126 MIREADTDGDGVIN 139
Score = 28.7 bits (61), Expect = 0.75
Identities = 19/54 (35%), Positives = 26/54 (48%), Gaps = 1/54 (1%)
Frame = +1
Query: 460 DEEGKID-SERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTA 618
D G ID +E L D + +EV EA+ D D GYI +L +LT+
Sbjct: 60 DGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTS 113
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 87.0 bits (206), Expect = 2e-18
Identities = 47/130 (36%), Positives = 70/130 (53%), Gaps = 2/130 (1%)
Frame = +1
Query: 160 SRKAKRTGSNVFSMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELD 339
++ AKR S F+ + Q+ E KEAF L+D D DG IG+ D++ SL + ASE ++
Sbjct: 28 AQAAKRASSGAFAQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSIN 87
Query: 340 EMVGEASGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFD--EEGKIDSERLRHALMTW 513
M + PIN LT + + S +D ++ AF TFD + GKI +R AL +
Sbjct: 88 HMFESINPPINLAAFLTAMGSMLCRISPRND-LLEAFSTFDDTQSGKIPISTMRDALSSM 146
Query: 514 GDKFSADEVD 543
GD+ EV+
Sbjct: 147 GDRMDPQEVE 156
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 66.1 bits (154), Expect = 4e-12
Identities = 40/140 (28%), Positives = 72/140 (51%), Gaps = 2/140 (1%)
Frame = +1
Query: 205 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQL 384
S++Q E KEAF L D DKDG+I + + + SLG ++ EL ++ E I+ +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63
Query: 385 LTLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYDQ 558
++ +N++ E++ I AF+ FD++ G I++ + + T G+K S +EV +
Sbjct: 64 MSFVSNKLRETESEEE-YIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQE 122
Query: 559 MDIDDKGYIDTTKLIAMLTA 618
D + G D + + A
Sbjct: 123 ADPTNSGSFDYYDFVQRIMA 142
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 54.4 bits (125), Expect = 1e-08
Identities = 36/142 (25%), Positives = 68/142 (47%), Gaps = 6/142 (4%)
Frame = +1
Query: 205 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 372
+++Q + EAF+L D DKD I ++LRA +LG A + E+ +++ + G +
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQ 91
Query: 373 FTQLLTLFANRMSGGSDEDDVVINAFKTF--DEEGKIDSERLRHALMTWGDKFSADEVDE 546
+ + ++ D + + AF+ F DE GKI LR + E++
Sbjct: 92 MEDFVRVMTEKIV-ERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEA 150
Query: 547 AYDQMDIDDKGYIDTTKLIAML 612
++ D+D G I+ + IA++
Sbjct: 151 MIEEFDLDQDGEINEQEFIAIM 172
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 47.6 bits (108), Expect = 2e-06
Identities = 34/135 (25%), Positives = 63/135 (46%), Gaps = 4/135 (2%)
Frame = +1
Query: 226 FKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANR 405
+K+AF L D G I K + + G+ + E+ E+ ++ Q L + NR
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTLPAEVDMEQFLQVL-NR 66
Query: 406 MSGGS--DEDDVVINAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDD 573
+G + + + F+ FD++ G I LR+ L + G+K S +E+DE + + D
Sbjct: 67 PNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKD 126
Query: 574 KGYIDTTKLIAMLTA 618
G ++ + M+ A
Sbjct: 127 -GMVNYHDFVQMILA 140
Score = 40.7 bits (91), Expect = 2e-04
Identities = 18/42 (42%), Positives = 26/42 (61%)
Frame = +1
Query: 223 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 348
EF + FQ+ D D G+IG +LR SLG S +E+DE++
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELL 119
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 28.3 bits (60), Expect = 1.00
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = +1
Query: 532 DEVDEAYDQMDIDDKGYID 588
+E +EA+D D+ KGYID
Sbjct: 12 EEAEEAFDLFDVTHKGYID 30
Score = 27.1 bits (57), Expect = 2.3
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = +1
Query: 223 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 348
E +EAF L D G I DLR + LG ++++L M+
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLML 54
>SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 673
Score = 25.4 bits (53), Expect = 7.0
Identities = 17/53 (32%), Positives = 26/53 (49%)
Frame = +1
Query: 463 EEGKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTAP 621
+E D L A WG + A+ ++ DQ D+D +D K +A L+AP
Sbjct: 601 DEVNTDESTLSTAKTVWGTRALANIQNDTDDQDDLDG-WKVDWDK-VAQLSAP 651
>SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1274
Score = 25.0 bits (52), Expect = 9.3
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = +1
Query: 214 QVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASE 327
+ A+FK+ F DKD + + +L A + L +LA +
Sbjct: 212 EYAKFKDKFPEAPLDKDNLQTQKELEARIECLKQLAEK 249
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,836,377
Number of Sequences: 5004
Number of extensions: 28241
Number of successful extensions: 137
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 123
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 132
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 287744314
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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