Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30670
(479 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 67 1e-12
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 64 1e-11
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 48 1e-06
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 29 0.36
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 27 1.5
SPBC215.06c |||human LYHRT homolog|Schizosaccharomyces pombe|chr... 27 1.9
SPAC27E2.06c |||methionine-tRNA ligase, mitochondrial|Schizosacc... 26 2.6
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 26 3.4
SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces pombe... 25 4.5
SPBC13G1.03c |pex14||peroxisomal membrane anchor protein|Schizos... 25 7.8
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 66.9 bits (156), Expect = 1e-12
Identities = 46/147 (31%), Positives = 73/147 (49%), Gaps = 3/147 (2%)
Frame = +1
Query: 43 MSDLSKNDVERASF--AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXX 216
M+ + D + A F AFS++D + G I + LG ++R+L +PT A +
Sbjct: 1 MTTRNLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDAD 60
Query: 217 XXXXXXXX-FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALCEKL 393
FL + ++ KD D E+ E K++DK+ NG + ELTH L +L E+L
Sbjct: 61 GNGTIDFTEFLTMMARKMKDTDNE--EEVREAFKVFDKDGNGYITVEELTHVLTSLGERL 118
Query: 394 DDSEVAEVTKDCMDPEDDDGMIPYAAF 474
EVA++ ++ D DG+I Y F
Sbjct: 119 SQEEVADMIREA--DTDGDGVINYEEF 143
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 63.7 bits (148), Expect = 1e-11
Identities = 42/131 (32%), Positives = 66/131 (50%)
Frame = +1
Query: 85 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXXFLPIYSQA 264
AFS++D G G+I ++GDLLRA NPTLA I FL + ++
Sbjct: 11 AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI---TEIESTLPAEVDMEQFLQVLNRP 67
Query: 265 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALCEKLDDSEVAEVTKDCMDPED 444
G E+F++ +++DK+ G++ EL + L +L EKL + E+ E+ K
Sbjct: 68 NGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PV 124
Query: 445 DDGMIPYAAFL 477
DGM+ Y F+
Sbjct: 125 KDGMVNYHDFV 135
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 47.6 bits (108), Expect = 1e-06
Identities = 34/141 (24%), Positives = 65/141 (46%)
Frame = +1
Query: 55 SKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXX 234
SK + AF +YD + G I ++G +LR+L N T A +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63
Query: 235 XXFLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALCEKLDDSEVAE 414
F+ + K ++ + E++++ +++DK+ +G + A+ + L EKL D+EV
Sbjct: 64 MSFV-----SNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQL 118
Query: 415 VTKDCMDPEDDDGMIPYAAFL 477
+ ++ DP + G Y F+
Sbjct: 119 MVQEA-DP-TNSGSFDYYDFV 137
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 29.1 bits (62), Expect = 0.36
Identities = 28/132 (21%), Positives = 51/132 (38%), Gaps = 1/132 (0%)
Frame = +1
Query: 85 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXX-FLPIYSQ 261
AF ++D + ID L +RAL N + + F+ + ++
Sbjct: 42 AFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMTE 101
Query: 262 AKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALCEKLDDSEVAEVTKDCMDPE 441
++D E+ +L+D +E G + L L E +DD E+ + ++
Sbjct: 102 KIVERDP--LEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEF--DL 157
Query: 442 DDDGMIPYAAFL 477
D DG I F+
Sbjct: 158 DQDGEINEQEFI 169
Score = 26.6 bits (56), Expect = 1.9
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = +1
Query: 52 LSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSN 168
+ ++ +E AF ++D + GKI NL + + LN N
Sbjct: 104 VERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNEN 142
Score = 25.8 bits (54), Expect = 3.4
Identities = 14/58 (24%), Positives = 29/58 (50%)
Frame = +1
Query: 253 YSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALCEKLDDSEVAEVTKD 426
Y+ + + + +D E KL+D +++ + EL + AL + SEV ++ +D
Sbjct: 24 YAPLRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRD 81
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 27.1 bits (57), Expect = 1.5
Identities = 13/29 (44%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
Frame = +1
Query: 43 MSDLSKNDVER-ASFAFSIYDFEGKGKID 126
+++L DV R SF F +YDF G G +D
Sbjct: 605 IAELKFRDVMRNISFIFELYDFNGDGFMD 633
>SPBC215.06c |||human LYHRT homolog|Schizosaccharomyces pombe|chr
2|||Manual
Length = 178
Score = 26.6 bits (56), Expect = 1.9
Identities = 19/92 (20%), Positives = 41/92 (44%), Gaps = 1/92 (1%)
Frame = +2
Query: 29 KHHTK*ATSARTTLKGRLSPSQSTTLKAKVR-SMPSTLAIS*ERSTQTPHWQPSRNSVVQ 205
++HT T A+ KG P++ KAK+ + ++ +S Q P+++S+ +
Sbjct: 43 RNHTSCMTEAQRYQKGLYRPTKKELKKAKMNGNAVNSKELSPNTDNQNTPAGPTKHSLDE 102
Query: 206 RRRARSCSHSKSSFPSTAKQRKTKTRERMKIS 301
+ + SK S+ ++ + +IS
Sbjct: 103 NEKDKENKKSKKETVSSPAEQLLALTQNQEIS 134
>SPAC27E2.06c |||methionine-tRNA ligase,
mitochondrial|Schizosaccharomyces pombe|chr 1|||Manual
Length = 539
Score = 26.2 bits (55), Expect = 2.6
Identities = 12/39 (30%), Positives = 23/39 (58%)
Frame = +2
Query: 317 CMTRTKTVSCLALSSHTRFLHYVRSLTTVKSLKSQRTAW 433
C +K + LA++++T+F H++R+ T K S + W
Sbjct: 88 CDRNSKRFADLAVAANTKFTHFIRT-TNPKHQASVQEFW 125
>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1142
Score = 25.8 bits (54), Expect = 3.4
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = +1
Query: 379 LCEKLDDSEVAEVTKDCMDPED 444
L EK+ D + + DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798
>SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 551
Score = 25.4 bits (53), Expect = 4.5
Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 2/75 (2%)
Frame = +2
Query: 62 TTLKGRLSPSQSTTLKA-KVRSMPS-TLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSHS 235
T +PS L+A + S+P+ +L S E + + +R ++ ++ S +
Sbjct: 309 TEYSSNTAPSNWIALEALPLVSIPNESLDESDELAESLSDSEAARLQLLGIKKQESVAKK 368
Query: 236 KSSFPSTAKQRKTKT 280
KSSFPST K + T
Sbjct: 369 KSSFPSTIKDQPNLT 383
>SPBC13G1.03c |pex14||peroxisomal membrane anchor
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 286
Score = 24.6 bits (51), Expect = 7.8
Identities = 17/48 (35%), Positives = 22/48 (45%)
Frame = -1
Query: 182 VASVGFELSALRRSPRLKASILPLPSKS*IEKAKDALSTSFLLRSLIL 39
V S GF SA + A + PS++ E K+AL FL IL
Sbjct: 77 VISTGFAWSAYSLVKKYIAPMFRAPSQNAYEADKNALDAKFLEAHKIL 124
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.316 0.135 0.388
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,868,708
Number of Sequences: 5004
Number of extensions: 34024
Number of successful extensions: 115
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 109
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 111
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 184020746
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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