Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30668
         (603 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    71   1e-13
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    71   1e-13
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    57   2e-09
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    31   0.13 
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo...    27   2.1  
SPBC215.06c |||human LYHRT homolog|Schizosaccharomyces pombe|chr...    27   2.8  
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy...    26   4.9  
SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces pombe...    25   6.4  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 70.9 bits (166), Expect = 1e-13
 Identities = 47/151 (31%), Positives = 76/151 (50%), Gaps = 3/151 (1%)
 Frame = +2

Query: 17  MSDLSKNDVERASF--AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXX 190
           M+  +  D + A F  AFS++D +  G I +  LG ++R+L  +PT A +          
Sbjct: 1   MTTRNLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDAD 60

Query: 191 XXXXXXXX-FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKL 367
                    FL + ++  KD D    E+  E  K++DK+ NG +   ELTH L +LGE+L
Sbjct: 61  GNGTIDFTEFLTMMARKMKDTDNE--EEVREAFKVFDKDGNGYITVEELTHVLTSLGERL 118

Query: 368 DDSEVAEVTKDCMDPEDDDGMIPYAAFLKKV 460
              EVA++ ++     D DG+I Y  F + +
Sbjct: 119 SQEEVADMIREA--DTDGDGVINYEEFSRVI 147


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 70.9 bits (166), Expect = 1e-13
 Identities = 44/136 (32%), Positives = 71/136 (52%)
 Frame = +2

Query: 59  AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXXFLPIYSQA 238
           AFS++D  G G+I   ++GDLLRA   NPTLA I                  FL + ++ 
Sbjct: 11  AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI---TEIESTLPAEVDMEQFLQVLNRP 67

Query: 239 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPED 418
                 G  E+F++  +++DK+  G++   EL + L +LGEKL + E+ E+ K       
Sbjct: 68  NGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PV 124

Query: 419 DDGMIPYAAFLKKVMA 466
            DGM+ Y  F++ ++A
Sbjct: 125 KDGMVNYHDFVQMILA 140


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 57.2 bits (132), Expect = 2e-09
 Identities = 37/146 (25%), Positives = 71/146 (48%)
 Frame = +2

Query: 29  SKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXX 208
           SK   +    AF +YD +  G I   ++G +LR+L  N T A +                
Sbjct: 4   SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63

Query: 209 XXFLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAE 388
             F+     + K ++  + E++++  +++DK+ +G +  A+    +  LGEKL D+EV  
Sbjct: 64  MSFV-----SNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQL 118

Query: 389 VTKDCMDPEDDDGMIPYAAFLKKVMA 466
           + ++  DP  + G   Y  F++++MA
Sbjct: 119 MVQEA-DP-TNSGSFDYYDFVQRIMA 142


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 31.1 bits (67), Expect = 0.13
 Identities = 29/136 (21%), Positives = 53/136 (38%), Gaps = 1/136 (0%)
 Frame = +2

Query: 59  AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXX-FLPIYSQ 235
           AF ++D +    ID   L   +RAL  N   + +                   F+ + ++
Sbjct: 42  AFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMTE 101

Query: 236 AKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPE 415
              ++D    E+     +L+D +E G +    L      L E +DD E+  + ++     
Sbjct: 102 KIVERDP--LEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEF--DL 157

Query: 416 DDDGMIPYAAFLKKVM 463
           D DG I    F+  +M
Sbjct: 158 DQDGEINEQEFIAIMM 173



 Score = 29.5 bits (63), Expect = 0.40
 Identities = 15/58 (25%), Positives = 30/58 (51%)
 Frame = +2

Query: 227 YSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKD 400
           Y+  + +  +   +D  E  KL+D +++  +   EL   + ALG   + SEV ++ +D
Sbjct: 24  YAPLRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRD 81



 Score = 26.6 bits (56), Expect = 2.8
 Identities = 12/39 (30%), Positives = 21/39 (53%)
 Frame = +2

Query: 26  LSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSN 142
           + ++ +E    AF ++D +  GKI   NL  + + LN N
Sbjct: 104 VERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNEN 142


>SPBC215.01 ||SPBC3B9.20|GTPase activating
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 834

 Score = 27.1 bits (57), Expect = 2.1
 Identities = 13/29 (44%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
 Frame = +2

Query: 17  MSDLSKNDVER-ASFAFSIYDFEGKGKID 100
           +++L   DV R  SF F +YDF G G +D
Sbjct: 605 IAELKFRDVMRNISFIFELYDFNGDGFMD 633


>SPBC215.06c |||human LYHRT homolog|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 178

 Score = 26.6 bits (56), Expect = 2.8
 Identities = 19/92 (20%), Positives = 41/92 (44%), Gaps = 1/92 (1%)
 Frame = +3

Query: 3   KHHTK*ATSARTTLKGRLSPSQSTTLKAKVR-SMPSTLAIS*ERSTQTPHWQPSRNSVVQ 179
           ++HT   T A+   KG   P++    KAK+  +  ++  +S     Q     P+++S+ +
Sbjct: 43  RNHTSCMTEAQRYQKGLYRPTKKELKKAKMNGNAVNSKELSPNTDNQNTPAGPTKHSLDE 102

Query: 180 RRRARSCSHSKSSFPSTAKQRKTKTRERMKIS 275
             + +    SK    S+  ++     +  +IS
Sbjct: 103 NEKDKENKKSKKETVSSPAEQLLALTQNQEIS 134


>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1142

 Score = 25.8 bits (54), Expect = 4.9
 Identities = 9/22 (40%), Positives = 14/22 (63%)
 Frame = +2

Query: 353 LGEKLDDSEVAEVTKDCMDPED 418
           L EK+ D +   +  DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798


>SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 551

 Score = 25.4 bits (53), Expect = 6.4
 Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 2/75 (2%)
 Frame = +3

Query: 36  TTLKGRLSPSQSTTLKA-KVRSMPS-TLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSHS 209
           T      +PS    L+A  + S+P+ +L  S E +      + +R  ++  ++  S +  
Sbjct: 309 TEYSSNTAPSNWIALEALPLVSIPNESLDESDELAESLSDSEAARLQLLGIKKQESVAKK 368

Query: 210 KSSFPSTAKQRKTKT 254
           KSSFPST K +   T
Sbjct: 369 KSSFPSTIKDQPNLT 383


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,304,673
Number of Sequences: 5004
Number of extensions: 43372
Number of successful extensions: 129
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 121
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 124
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 264253462
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)