Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30655
(731 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g14185.1 68414.m01678 glucose-methanol-choline (GMC) oxidored... 56 2e-08
At1g12570.1 68414.m01459 glucose-methanol-choline (GMC) oxidored... 56 2e-08
At1g72970.1 68414.m08439 glucose-methanol-choline (GMC) oxidored... 56 3e-08
At1g14190.1 68414.m01679 glucose-methanol-choline (GMC) oxidored... 55 4e-08
At3g56060.1 68416.m06229 glucose-methanol-choline (GMC) oxidored... 54 1e-07
At5g51950.1 68418.m06447 glucose-methanol-choline (GMC) oxidored... 53 2e-07
At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative / (R... 52 3e-07
At5g51930.1 68418.m06442 glucose-methanol-choline (GMC) oxidored... 49 4e-06
At2g37580.1 68415.m04610 zinc finger (C3HC4-type RING finger) fa... 32 0.34
At5g11990.1 68418.m01402 proline-rich family protein contains pr... 31 0.60
At5g44390.1 68418.m05435 FAD-binding domain-containing protein s... 30 1.4
At5g38280.1 68418.m04615 serine/threonine protein kinase (PR5K) ... 29 4.2
At5g01310.1 68418.m00043 basic helix-loop-helix (bHLH) family pr... 29 4.2
At2g34910.1 68415.m04286 expressed protein 29 4.2
At1g22220.1 68414.m02778 F-box family protein contains F-box dom... 29 4.2
At4g19380.1 68417.m02853 alcohol oxidase-related similar to long... 28 5.6
At3g50040.1 68416.m05471 expressed protein putative protein - Ar... 28 5.6
At3g04940.1 68416.m00536 cysteine synthase, putative / O-acetyls... 28 5.6
At1g73480.1 68414.m08507 hydrolase, alpha/beta fold family prote... 28 7.3
At2g19400.1 68415.m02263 protein kinase, putative contains prote... 27 9.7
At1g05270.1 68414.m00533 TraB family protein contains Pfam domai... 27 9.7
>At1g14185.1 68414.m01678 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 503
Score = 56.4 bits (130), Expect = 2e-08
Identities = 36/114 (31%), Positives = 56/114 (49%), Gaps = 8/114 (7%)
Frame = +1
Query: 250 VIKNAIATKIVFKPGTNIVSGVLL----NKGGRDIAVNV---RKEVVVSAGAINSPQLLL 408
V+ NA I+F GV + V+V R EV+++AGA+ SPQ+LL
Sbjct: 219 VLLNATVKSIIFDANKTRAVGVRFMESDGNSSKSYKVHVEQHRGEVILAAGALGSPQILL 278
Query: 409 LSGIGPRKHLEDLNIEVKADL-PVGENLQDHLFVPVFYTKPGDKKATTLPNIIS 567
LSGIGP HL D +I V +L VG+ + D+ + + + P +++
Sbjct: 279 LSGIGPENHLNDFDIPVIVNLKEVGKQMSDNPAISLLVDRFSQNLTVDPPQVVA 332
>At1g12570.1 68414.m01459 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 572
Score = 56.4 bits (130), Expect = 2e-08
Identities = 42/125 (33%), Positives = 68/125 (54%), Gaps = 9/125 (7%)
Frame = +1
Query: 181 KGGTRFSTARAFLSPIKDRKNLHVIKNAIATKIVFKP-GTN--IVSGVLL-NKGGRDIAV 348
+ G R + A L D K + V+ +A +I+F+ GT I +GV+ ++ G+
Sbjct: 206 RNGNRHTAAD--LLEYADPKGITVLLHATVHRILFRTRGTTKPIANGVVYRDRTGQAHRA 263
Query: 349 NVRK----EVVVSAGAINSPQLLLLSGIGPRKHLEDLNIEVKADLP-VGENLQDHLFVPV 513
+++ E+++SAG + SPQLL+LSG+GP L+ NI V D P VG+ + D+ V
Sbjct: 264 YLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNITVVMDQPHVGQGMYDNPMNAV 323
Query: 514 FYTKP 528
F P
Sbjct: 324 FVPSP 328
>At1g72970.1 68414.m08439 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 594
Score = 55.6 bits (128), Expect = 3e-08
Identities = 51/140 (36%), Positives = 74/140 (52%), Gaps = 8/140 (5%)
Frame = +1
Query: 103 VELGLKNLTDCNGDSQIGVMKSFTTTKGGTRFSTARAFLSPIKDRKNLHVIKNAIATKIV 282
+E+G++ D G T R TA L+ +K L V+ A KIV
Sbjct: 196 LEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYANPQK-LRVLIYATVQKIV 254
Query: 283 FKP-GTNI-VSGVLLN--KGGRDIAV-NVRK--EVVVSAGAINSPQLLLLSGIGPRKHLE 441
F GT V+GV+ KG + A+ + RK EV++S+GAI SPQ+L+LSGIGP+K L+
Sbjct: 255 FDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIGSPQMLMLSGIGPKKELQ 314
Query: 442 DLNIEVKADLP-VGENLQDH 498
L I V + VG+ + D+
Sbjct: 315 RLKIPVVLENEHVGKGMADN 334
>At1g14190.1 68414.m01679 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 501
Score = 55.2 bits (127), Expect = 4e-08
Identities = 27/70 (38%), Positives = 42/70 (60%), Gaps = 1/70 (1%)
Frame = +1
Query: 355 RKEVVVSAGAINSPQLLLLSGIGPRKHLEDLNIEVKADL-PVGENLQDHLFVPVFYTKPG 531
R EV+++AGA+ SPQ+LLLSGIGP HL+D +I V +L VG + D+ + + +
Sbjct: 241 RGEVILAAGALGSPQILLLSGIGPENHLKDFDIPVIVNLKEVGRKMSDNPAISLLVDRFS 300
Query: 532 DKKATTLPNI 561
+ P +
Sbjct: 301 QNRTLEPPQV 310
>At3g56060.1 68416.m06229 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 577
Score = 53.6 bits (123), Expect = 1e-07
Identities = 29/58 (50%), Positives = 37/58 (63%), Gaps = 2/58 (3%)
Frame = +1
Query: 361 EVVVSAGAINSPQLLLLSGIGPRKHLEDLNIE-VKADLP-VGENLQDHLFVPVFYTKP 528
EV++SAGAI SPQLL+LSG+GP HL + V D P VG+ + D+ PVF P
Sbjct: 274 EVILSAGAIASPQLLMLSGVGPAAHLAAYRVNPVIVDQPMVGQGMGDNPMNPVFIPSP 331
>At5g51950.1 68418.m06447 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 586
Score = 53.2 bits (122), Expect = 2e-07
Identities = 32/93 (34%), Positives = 50/93 (53%), Gaps = 2/93 (2%)
Frame = +1
Query: 337 DIAVNVRKEVVVSAGAINSPQLLLLSGIGPRKHLEDLNIE-VKADLP-VGENLQDHLFVP 510
++ N EV++SAGAI SPQLL+LSGIGP HL I+ + D P VG+ + D+
Sbjct: 264 ELEKNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNA 323
Query: 511 VFYTKPGDKKATTLPNIISTFIEYFLHNTGDLI 609
+F P + + + + T E ++ +I
Sbjct: 324 IFIPSPTPVEVSLIQVVGITKFESYIEGASGVI 356
>At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative /
(R)-oxynitrilase, putative similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|52707];
contains Pfam protile PF00732 GMC oxidoreductase
Length = 552
Score = 52.4 bits (120), Expect = 3e-07
Identities = 27/49 (55%), Positives = 34/49 (69%), Gaps = 1/49 (2%)
Frame = +1
Query: 355 RKEVVVSAGAINSPQLLLLSGIGPRKHLEDLNIEVKADLP-VGENLQDH 498
R EV++SAGA+ SPQLL LSGIGPR +L I V D P VG+ + D+
Sbjct: 282 RGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDN 330
>At5g51930.1 68418.m06442 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 582
Score = 48.8 bits (111), Expect = 4e-06
Identities = 25/56 (44%), Positives = 37/56 (66%), Gaps = 2/56 (3%)
Frame = +1
Query: 337 DIAVNVRKEVVVSAGAINSPQLLLLSGIGPRKHLEDLNIE-VKADLP-VGENLQDH 498
++A EV++SAGA+ SPQLL+LSG+GP HLE + + D P VG+ + D+
Sbjct: 285 NLANKATNEVILSAGALGSPQLLMLSGVGPAVHLEAHGVNPLVLDQPMVGQGMADN 340
>At2g37580.1 68415.m04610 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type
Length = 235
Score = 32.3 bits (70), Expect = 0.34
Identities = 18/42 (42%), Positives = 22/42 (52%)
Frame = -1
Query: 380 PAETTTSFLTFTAISRPPLFNKTPLTMLVPGLKTILVAIAFL 255
P T TS TF I PP F P ++ + LK I V IAF+
Sbjct: 31 PGTTITSNSTFIIIGPPPPFPAPPRSIDLTPLKLIFVVIAFV 72
>At5g11990.1 68418.m01402 proline-rich family protein contains
proline-rich extensin domains, INTERPRO:IPR002965
Length = 181
Score = 31.5 bits (68), Expect = 0.60
Identities = 17/35 (48%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
Frame = +2
Query: 425 LASTWKT*TLKLKPICLSE-KTCRTICSYPSSIPN 526
+AS+ T L P+C+SE TC TICS P S P+
Sbjct: 16 VASSPSDQTNVLTPLCISECSTCPTICSPPPSKPS 50
>At5g44390.1 68418.m05435 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 542
Score = 30.3 bits (65), Expect = 1.4
Identities = 16/46 (34%), Positives = 25/46 (54%)
Frame = +1
Query: 475 VGENLQDHLFVPVFYTKPGDKKATTLPNIISTFIEYFLHNTGDLID 612
+ +NL D LF+ VF+T G+K T + +I FL G L++
Sbjct: 286 IADNLVDELFLRVFFTVSGNKANKT---VTMAYIGQFLGEKGTLME 328
>At5g38280.1 68418.m04615 serine/threonine protein kinase (PR5K)
identical to receptor serine/threonine kinase PR5K
gi|1235680|gb|AAC49208
Length = 665
Score = 28.7 bits (61), Expect = 4.2
Identities = 18/49 (36%), Positives = 22/49 (44%)
Frame = -3
Query: 408 QQQLWGVNSTSGNDNFFSYIYSNITPTFV*QNSTDYVGTGLKNDFSGDS 262
Q L+ N + +SY Y N TF NS +YV T ND S S
Sbjct: 209 QPTLYSTNFKNECPLAYSYAYDNENNTFRCSNSPNYVITFCPNDISSMS 257
>At5g01310.1 68418.m00043 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain, weak hit to PF01661: Appr-1-p
processing enzyme family
Length = 912
Score = 28.7 bits (61), Expect = 4.2
Identities = 30/123 (24%), Positives = 52/123 (42%), Gaps = 5/123 (4%)
Frame = +1
Query: 43 LSVASDDNMHEIEDLIIK-AAVELGLKNLTDCNGDSQIGVMKSFTTTKG--GTRFSTARA 213
L ASD + + E+ + K L L +L+ G + ++K+ + K +F T
Sbjct: 472 LEKASDIIVEKAEEFLSKLGTARLVLVDLS--RGSKILSLVKAKASQKNIDSAKFFTFVG 529
Query: 214 FLSPIKDRKNLHVIKNAIATKIVFKPGTNIVSGVLLNKGGRDI--AVNVRKEVVVSAGAI 387
++ ++ LH A AT KPG V+ + G D+ A VR ++ A+
Sbjct: 530 DITKLRSEGGLHCNVIANATNWRLKPGGGGVNAAIFKAAGPDLETATRVRANTLLPGKAV 589
Query: 388 NSP 396
P
Sbjct: 590 VVP 592
>At2g34910.1 68415.m04286 expressed protein
Length = 288
Score = 28.7 bits (61), Expect = 4.2
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = +1
Query: 601 DLIDTSPHRVIAFENTTDPNSPAS 672
DL +TSP R + F TT + PAS
Sbjct: 240 DLAETSPQRRVRFSTTTSDSCPAS 263
>At1g22220.1 68414.m02778 F-box family protein contains F-box domain
Pfam:PF00646
Length = 314
Score = 28.7 bits (61), Expect = 4.2
Identities = 13/44 (29%), Positives = 20/44 (45%)
Frame = -1
Query: 500 KWSCKFSPTGKSALTSMFRSSKCLRGPIPESNNSCGELIAPAET 369
KW +F T KS + FRS+ + P+ S E + +T
Sbjct: 122 KWKAEFGKTLKSCVIVAFRSAGTVSSPVAVEGESDAEFVTGLKT 165
>At4g19380.1 68417.m02853 alcohol oxidase-related similar to long
chain fatty alcohol oxidase from Candida cloacae
[GI:6983581], Candida tropicalis [GI:6983594]
Length = 726
Score = 28.3 bits (60), Expect = 5.6
Identities = 19/82 (23%), Positives = 40/82 (48%), Gaps = 2/82 (2%)
Frame = +1
Query: 181 KGGTRFSTARAFLSPIKDRKNLHVIKNAIATKIVF--KPGTNIVSGVLLNKGGRDIAVNV 354
K G + T+ +L + + N ++ AT++++ + G + + G +I V
Sbjct: 391 KKGQKQGTSETWLVDLVESDNGLILPGCQATEVMYDCEQGKKKKATGVAFAFGEEIYVVE 450
Query: 355 RKEVVVSAGAINSPQLLLLSGI 420
+ +V+ GA+ +P LL SG+
Sbjct: 451 SRVTIVACGALRTPHLLKRSGL 472
>At3g50040.1 68416.m05471 expressed protein putative protein -
Arabidopsis thaliana, EMBL:AL035601
Length = 421
Score = 28.3 bits (60), Expect = 5.6
Identities = 25/114 (21%), Positives = 46/114 (40%)
Frame = -3
Query: 591 VQEVFNESRDNIRQGSSFLVSRFGIEDGYEQMVLQVFSDRQIGFNFNV*VFQVLARSNSG 412
V+E+ +++R ++ + +++ + E+ L+ F + I F N V R
Sbjct: 133 VKEIQSQARGYEKEVKDYYLTK---QFDLEKSKLEGFDGKSIPFRENNQRRNVFKRGRRK 189
Query: 411 EQQQLWGVNSTSGNDNFFSYIYSNITPTFV*QNSTDYVGTGLKNDFSGDSVLDD 250
++ V + N N FSY + Q GTG K D++ DD
Sbjct: 190 RVEETTDVAAYMSNHNLFSYADKRVPVNVKGQYLDSDFGTGRKATGKQDAIEDD 243
>At3g04940.1 68416.m00536 cysteine synthase, putative /
O-acetylserine (thiol)-lyase, putative / O-acetylserine
sulfhydrylase, putative similar to O-acetylserine(thiol)
lyase (EC 4.2.99.8) [Brassica juncea] GI:2245144;
contains Pfam profile PF00291: Pyridoxal-phosphate
dependent enzyme
Length = 324
Score = 28.3 bits (60), Expect = 5.6
Identities = 25/78 (32%), Positives = 34/78 (43%), Gaps = 2/78 (2%)
Frame = +1
Query: 184 GGTRFSTARAFLSPIKDRKNLHVIKNAIATKIVFKPGTNIVSGVLLNKGGRDIAVNVRKE 363
GGT + R KD K V A KPGT+++ G+ ++ NV E
Sbjct: 186 GGTLSGSGRFLKEKNKDFKVYGVEPTESAVISGGKPGTHLIQGIGAGLIPDNLDFNVLDE 245
Query: 364 V--VVSAGAINSPQLLLL 411
V V S AI + +LL L
Sbjct: 246 VIQVTSVEAIETAKLLAL 263
>At1g73480.1 68414.m08507 hydrolase, alpha/beta fold family protein
low similarity to monoglyceride lipase from [Homo
sapiens] GI:14594904, [Mus musculus] GI:2632162;
contains Pfam profile PF00561: hydrolase, alpha/beta
fold family
Length = 463
Score = 27.9 bits (59), Expect = 7.3
Identities = 23/66 (34%), Positives = 31/66 (46%), Gaps = 2/66 (3%)
Frame = +1
Query: 34 GGYLSVASDDNMHEIEDLIIKAAVELGLKN-LTDCNGD-SQIGVMKSFTTTKGGTRFSTA 207
GG SV+ + D + EL +K L D GD S + FTT +G T FS +
Sbjct: 143 GGESSVSPPSVPAAVVDGEVAVRRELAIKRVLEDEGGDGSSVRDYSLFTTKRGDTLFSQS 202
Query: 208 RAFLSP 225
+ LSP
Sbjct: 203 WSPLSP 208
>At2g19400.1 68415.m02263 protein kinase, putative contains protein
kinase domain, Pfam:PF00069
Length = 527
Score = 27.5 bits (58), Expect = 9.7
Identities = 20/63 (31%), Positives = 26/63 (41%)
Frame = -3
Query: 429 ARSNSGEQQQLWGVNSTSGNDNFFSYIYSNITPTFV*QNSTDYVGTGLKNDFSGDSVLDD 250
AR+ SG W V+ T N NF Y Y N ++S D G+ S DS D
Sbjct: 454 ARTGSGPS---WKVSITPQNINFVGYTYRNFDAVRGSRHSLDIKGSVSPPRSSTDSTRSD 510
Query: 249 MEV 241
+
Sbjct: 511 SAI 513
>At1g05270.1 68414.m00533 TraB family protein contains Pfam domain
PF01963: TraB family
Length = 371
Score = 27.5 bits (58), Expect = 9.7
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +1
Query: 217 LSPIKDRKNLHVIKNAIATKIVFKPGTNIVSGVLLNK 327
L I K++ +K I++ V GT IVSG+LL++
Sbjct: 333 LMEIPSDKSVFTLKRIISSVAVAVAGTAIVSGILLSR 369
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,352,461
Number of Sequences: 28952
Number of extensions: 324781
Number of successful extensions: 986
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 944
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 986
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1604469728
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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