Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30651
(736 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 41 2e-04
SPBC1347.04 |tim54||TIM22 inner membrane protein import complex ... 29 0.52
SPBC29A10.03c |rlf2|SPBC365.19c|chromatin remodeling complex sub... 29 0.69
SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc... 29 0.91
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 29 0.91
SPAC12G12.02 ||SPAC630.01c|rRNA processing protein, unnamed|Schi... 28 1.2
SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosacc... 27 2.1
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 27 2.8
SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 27 2.8
SPAC6G9.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 27 2.8
SPBC4C3.05c |nuc1|rpa1|DNA-directed RNA polymerase I complex lar... 27 3.7
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 26 4.8
SPAC458.02c |||mRNP complex |Schizosaccharomyces pombe|chr 1|||M... 26 6.4
SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit Pst... 26 6.4
SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos... 26 6.4
SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces pomb... 25 8.5
SPBC11C11.02 |imp2||contractile ring protein Imp2|Schizosaccharo... 25 8.5
>SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1085
Score = 40.7 bits (91), Expect = 2e-04
Identities = 29/117 (24%), Positives = 63/117 (53%)
Frame = -1
Query: 721 KLETEKYDLEERQKRQDYDLKELKERQKQQLRHKALKKGLDPEALTGKHPPKIQVASKYE 542
K E ++ E+ +K+Q+ + ++ +E+QKQ+ R K LKK EA K + ++ + E
Sbjct: 640 KREEQRLKREQEKKQQELERQKREEKQKQKEREKKLKK-QQQEADREKMAREQRLREEEE 698
Query: 541 RRVDTRSYDDKKKLFEGDLEKLNKDFLEKVWQERAEQFGGRQKARLPKWFGERPGKK 371
+R+ ++KL + + E+ ++ LEK +E+ + ++A++ +F K+
Sbjct: 699 KRI-LEERKRREKLDKEEEERRRRELLEKESEEKERRL---REAKIAAFFAPNQTKE 751
Score = 27.5 bits (58), Expect = 2.1
Identities = 28/119 (23%), Positives = 55/119 (46%), Gaps = 5/119 (4%)
Frame = -1
Query: 694 EERQKRQDYDLKE-LKERQKQQLRHKALKKGLDPEALTGKHPPKIQVASKYERRVDTRSY 518
EERQ+R+ L E ++ + R + +K + + L + K Q + +R +
Sbjct: 610 EERQRREAERLAEQAAQKALEAKRQEEARKKREEQRLKREQEKKQQELERQKREEKQKQK 669
Query: 517 DDKKKL----FEGDLEKLNKDFLEKVWQERAEQFGGRQKARLPKWFGERPGKKKGELES 353
+ +KKL E D EK+ ++ + +E+ +++ +L K ER ++ E ES
Sbjct: 670 EREKKLKKQQQEADREKMAREQRLREEEEKRILEERKRREKLDKEEEERRRRELLEKES 728
>SPBC1347.04 |tim54||TIM22 inner membrane protein import complex
subunit Tim54|Schizosaccharomyces pombe|chr 2|||Manual
Length = 347
Score = 29.5 bits (63), Expect = 0.52
Identities = 20/61 (32%), Positives = 31/61 (50%), Gaps = 9/61 (14%)
Frame = -1
Query: 721 KLETEKYDL---EERQKRQDYDLKELKERQKQQLRHKALKKG------LDPEALTGKHPP 569
KLETEK + EE+++++ D K+ KE K + K ++ LTG+ PP
Sbjct: 173 KLETEKLEANNKEEKEEKEGKDDKDDKEDSNDTKNDKKISKNEVDSSLIEASPLTGQVPP 232
Query: 568 K 566
K
Sbjct: 233 K 233
>SPBC29A10.03c |rlf2|SPBC365.19c|chromatin remodeling complex
subunit Rlf2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 544
Score = 29.1 bits (62), Expect = 0.69
Identities = 22/88 (25%), Positives = 45/88 (51%), Gaps = 2/88 (2%)
Frame = -1
Query: 721 KLETEKYDLEERQKRQDYDLKELKERQKQQLRHKALKKGLDPEALTGKHPPKIQVASKYE 542
KLE E+ L+E+Q+R KE + Q+LR K + L E + K ++++ + +
Sbjct: 129 KLEKERIRLQEQQRR--------KEERDQKLREKEEAQRLRQEQILNKERQQLKLNNFFT 180
Query: 541 RRVDTRSYDDKKKLFE--GDLEKLNKDF 464
+ V+ R ++ + + +L + K+F
Sbjct: 181 KGVEKRIAPNENFVADKTDELNEFEKEF 208
>SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1190
Score = 28.7 bits (61), Expect = 0.91
Identities = 13/34 (38%), Positives = 22/34 (64%)
Frame = -1
Query: 700 DLEERQKRQDYDLKELKERQKQQLRHKALKKGLD 599
D+E+ Q Q + L+E ++++LRH L+K LD
Sbjct: 921 DMEDDQFSQRCERMALREAERRKLRHGKLQKHLD 954
>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 3655
Score = 28.7 bits (61), Expect = 0.91
Identities = 13/36 (36%), Positives = 19/36 (52%)
Frame = +1
Query: 406 VAPFACRRTVRPSLATPSRGSPCSVSLGHPRTVSFC 513
V+PF R + P A P R P + +GH V++C
Sbjct: 1236 VSPFK-ERLLSPIFAKPLRALPFHIQIGHIDAVNYC 1270
>SPAC12G12.02 ||SPAC630.01c|rRNA processing protein,
unnamed|Schizosaccharomyces pombe|chr 1|||Manual
Length = 183
Score = 28.3 bits (60), Expect = 1.2
Identities = 23/111 (20%), Positives = 48/111 (43%), Gaps = 2/111 (1%)
Frame = -1
Query: 727 IVKLETEKYDLEERQKRQDYDLKELKERQKQQLRHKALKKGLDPEALTG--KHPPKIQVA 554
+ +LE +K + ER K+ + ++ ER+ +QL + +D E K K Q+
Sbjct: 49 MAQLEHKKQKIFERYKKVRFFERKKAERRIKQLEKSLKDETMDDEKRKQCEKSMRKCQID 108
Query: 553 SKYERRVDTRSYDDKKKLFEGDLEKLNKDFLEKVWQERAEQFGGRQKARLP 401
Y + + L+ + ++ ++W E E+F +K ++P
Sbjct: 109 LMYIKEYPPLT--KYVSLYAEGTSEQTEETRNRIWAEMEERFNSGRKHKIP 157
>SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 830
Score = 27.5 bits (58), Expect = 2.1
Identities = 14/50 (28%), Positives = 28/50 (56%)
Frame = -1
Query: 718 LETEKYDLEERQKRQDYDLKELKERQKQQLRHKALKKGLDPEALTGKHPP 569
LE ++YD+EE D DL+EL ++ + + ++ ++ +A G+ P
Sbjct: 84 LELDRYDIEELD--DDNDLEELDIGARRAVDARLRRRDIELDAAAGRTKP 131
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 27.1 bits (57), Expect = 2.8
Identities = 23/98 (23%), Positives = 43/98 (43%), Gaps = 1/98 (1%)
Frame = -1
Query: 718 LETEKYDLEERQKRQDYDLKELKERQKQQLRHKALKKGLDPEALTGKHPPKIQVASKYER 539
L+ + D+E+ + EL E +Q RH+ L K + K I+ A++ ER
Sbjct: 371 LDVTEIDVEQAFPLLNVPDAELDEAGLRQKRHQRLMKANYDARVRAKAEKAIEEAAEAER 430
Query: 538 -RVDTRSYDDKKKLFEGDLEKLNKDFLEKVWQERAEQF 428
D R + + + + +K LEK+ + + +F
Sbjct: 431 AEADERLRLENFSTWVNEKRETHKILLEKISKNKRLKF 468
>SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1336
Score = 27.1 bits (57), Expect = 2.8
Identities = 11/28 (39%), Positives = 18/28 (64%)
Frame = -3
Query: 89 TKYFYYLYFSTKRR*QPMTIQ*TNLFHK 6
++ F Y+Y ST+R Q + I T++ HK
Sbjct: 938 SRMFSYIYVSTERMCQDVVISTTSILHK 965
>SPAC6G9.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 681
Score = 27.1 bits (57), Expect = 2.8
Identities = 12/36 (33%), Positives = 21/36 (58%)
Frame = +1
Query: 439 PSLATPSRGSPCSVSLGHPRTVSFCRRRIVCRHASR 546
PS+++ S S+SL + +SFC+ + CR+ R
Sbjct: 328 PSVSSSLSSSVASLSLQNSNILSFCKDQHGCRYLQR 363
>SPBC4C3.05c |nuc1|rpa1|DNA-directed RNA polymerase I complex large
subunit Nuc1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 1689
Score = 26.6 bits (56), Expect = 3.7
Identities = 19/60 (31%), Positives = 29/60 (48%), Gaps = 4/60 (6%)
Frame = -1
Query: 622 KALKKGLDPEALTGKHPPKI---QVASKYERRVDTRSYDDKKKLFEGDLE-KLNKDFLEK 455
KALKK + + K+PP V+ K++R VD + + KL E KL+ L +
Sbjct: 1113 KALKKPYKYDPVLDKYPPSRYLGSVSEKFQRAVDEYTQKNPDKLIASKKESKLDDSLLNE 1172
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 26.2 bits (55), Expect = 4.8
Identities = 9/32 (28%), Positives = 16/32 (50%)
Frame = +1
Query: 427 RTVRPSLATPSRGSPCSVSLGHPRTVSFCRRR 522
R + P P R P ++ +GH +++C R
Sbjct: 1294 RLLSPIFGKPLRALPFTIQIGHIDAITYCLHR 1325
>SPAC458.02c |||mRNP complex |Schizosaccharomyces pombe|chr
1|||Manual
Length = 468
Score = 25.8 bits (54), Expect = 6.4
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = -1
Query: 703 YDLEERQKRQDYDLKELKERQKQQLRHKALKK 608
Y+ E+R KRQ+ E + R+K++ R A +K
Sbjct: 242 YEREQRAKRQEQFRLERENREKEKRRIAAQRK 273
>SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit
Pst2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1075
Score = 25.8 bits (54), Expect = 6.4
Identities = 10/33 (30%), Positives = 19/33 (57%)
Frame = -1
Query: 733 ECIVKLETEKYDLEERQKRQDYDLKELKERQKQ 635
E + KLE E+Y+ + + + +K LK+ Q +
Sbjct: 391 EAMTKLEEERYEFDRHIEATSWTIKSLKKIQNR 423
>SPBC17D11.05 |tif32||translation initiation factor
eIF3a|Schizosaccharomyces pombe|chr 2|||Manual
Length = 932
Score = 25.8 bits (54), Expect = 6.4
Identities = 19/63 (30%), Positives = 26/63 (41%)
Frame = -3
Query: 692 REAKETGLRLKRAQRKTKAATEAQSSQEGSRPRSAHRQAPAQNSSSVQVREACRHTILRR 513
R+ KE K A+ +A EA + E R H Q AQ + + A R R
Sbjct: 776 RQRKEI-FEQKLAEAIREAEEEAARAAEEEANRELHEQEEAQKRAIEERTRAAREAKERE 834
Query: 512 QKE 504
Q+E
Sbjct: 835 QRE 837
>SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1254
Score = 25.4 bits (53), Expect = 8.5
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = -1
Query: 661 KELKERQKQQLRHKALKKGLDPEALTG 581
KE+K+RQ+ Q K +++GL +G
Sbjct: 461 KEIKDRQRTQKEEKVVQRGLTQVLTSG 487
>SPBC11C11.02 |imp2||contractile ring protein
Imp2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 670
Score = 25.4 bits (53), Expect = 8.5
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = -1
Query: 523 SYDDKKKLFEGDLEKLNKDFLEK 455
S DKKK+ +EK++KD L K
Sbjct: 120 SLKDKKKMIVSGIEKVHKDKLSK 142
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,912,814
Number of Sequences: 5004
Number of extensions: 30239
Number of successful extensions: 161
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 160
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 347244562
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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