Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30650
(719 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 301 8e-83
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 301 8e-83
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 301 8e-83
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 97 2e-21
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 54 2e-08
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 45 1e-05
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 3.6
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 26 6.2
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 26 6.2
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 8.2
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 301 bits (738), Expect = 8e-83
Identities = 136/178 (76%), Positives = 156/178 (87%)
Frame = +2
Query: 41 IGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRG 220
+GRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG
Sbjct: 262 VGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRG 321
Query: 221 YVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRT 400
V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+
Sbjct: 322 NVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRS 381
Query: 401 GKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 574
GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 382 GKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 301 bits (738), Expect = 8e-83
Identities = 136/178 (76%), Positives = 156/178 (87%)
Frame = +2
Query: 41 IGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRG 220
+GRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG
Sbjct: 262 VGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRG 321
Query: 221 YVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRT 400
V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+
Sbjct: 322 NVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRS 381
Query: 401 GKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 574
GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 382 GKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 301 bits (738), Expect = 8e-83
Identities = 136/178 (76%), Positives = 156/178 (87%)
Frame = +2
Query: 41 IGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRG 220
+GRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG
Sbjct: 262 VGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRG 321
Query: 221 YVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRT 400
V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+
Sbjct: 322 NVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRS 381
Query: 401 GKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 574
GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 382 GKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 97.5 bits (232), Expect = 2e-21
Identities = 57/180 (31%), Positives = 96/180 (53%), Gaps = 2/180 (1%)
Frame = +2
Query: 44 GRVETGVLKPGTIVVFAPANITTEVKSV-EMHHEALQEAVPGDNVGFNVKNVSVKELRRG 220
G++E G +K + V+ P N T EV ++ + E + ++ GD V V+ +++ G
Sbjct: 486 GKIEAGSIKKNSNVLVMPINQTLEVTAIYDEADEEISSSICGDQVRLRVRGDD-SDVQTG 544
Query: 221 YVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRT 400
YV +KN P F AQ+ +L P ++ GY+ V+ HTA FA++ K+D+ T
Sbjct: 545 YVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHKLDK-T 602
Query: 401 GKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG-VIKAVN 577
+ ++ P G I L P+C+E F+++ +GRF +RD TVAVG V+K ++
Sbjct: 603 NRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 54.4 bits (125), Expect = 2e-08
Identities = 49/176 (27%), Positives = 79/176 (44%), Gaps = 1/176 (0%)
Frame = +2
Query: 44 GRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQE-AVPGDNVGFNVKNVSVKELRRG 220
GRVE G ++ ++ + VK+V + + AV GD V + ++ V +LR G
Sbjct: 423 GRVEAGNVQVNQVLYDVSSQEDAYVKNVIRNSDPSSTWAVAGDTVTLQLADIEVNQLRPG 482
Query: 221 YVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRT 400
+ + +N P + F A++ + G I +G T VL H+ + K+
Sbjct: 483 DILSNYEN-PVRRVRSFVAEIQTFDIHGPILSGSTLVL-----HLGRTVTSVSLKIVTVN 536
Query: 401 GKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 568
K + + S K I L PLC+ +E P LGRF +R TVA G++K
Sbjct: 537 NKRSR-HIASRKRALVRISFLDGLFPLCLA--EECPALGRFILRRSGDTVAAGIVK 589
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 45.2 bits (102), Expect = 1e-05
Identities = 42/176 (23%), Positives = 75/176 (42%), Gaps = 2/176 (1%)
Frame = +2
Query: 44 GRVETGVLKPGTIV--VFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRR 217
GRVE G LK G + V +++ T V +EM + L AV GDN G ++++ ++L+R
Sbjct: 272 GRVERGTLKKGAEIEIVGYGSHLKTTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKR 331
Query: 218 GYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRR 397
G + P F A +L + T +D + + + +++ ++
Sbjct: 332 GMIVAQPGTVAPH--QKFKASFYILTK--EEGGRRTGFVDKYRPQLYSRTSDVTVEL--- 384
Query: 398 TGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVI 565
T + K + GD + P+ +E Q RF VR+ TV ++
Sbjct: 385 THPDPNDSDKMVMPGDNVEMICTLIHPIVIEKGQ------RFTVREGGSTVGTALV 434
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 3.6
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -2
Query: 211 QFLDGHVLYVETYIVSRYSFLESFV 137
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.8 bits (54), Expect = 6.2
Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
Frame = -3
Query: 618 SAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAA 445
S +VTLPPPAS ++ T T T + S ++ G+ + +++ + ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241
Query: 444 SPDLMDFGLTSVDLPVRRSTFSLIS 370
S L +TS PV S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 25.8 bits (54), Expect = 6.2
Identities = 10/39 (25%), Positives = 22/39 (56%)
Frame = -2
Query: 235 VTSNITTTQFLDGHVLYVETYIVSRYSFLESFVVHLHRF 119
+T ++ + L H+ +++ SR +FL ++H+H F
Sbjct: 1012 LTQSLQSFSHLSNHIEVLDSTRQSRLTFLCHLILHMHGF 1050
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 8.2
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = -3
Query: 453 MAASPDLMDFGLTSVDLPVRRSTFS 379
++ +PDL D L+SVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,973,085
Number of Sequences: 5004
Number of extensions: 61340
Number of successful extensions: 175
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 337208592
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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