BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30650 (719 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 286 1e-77 At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 286 1e-77 At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 286 1e-77 At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 286 1e-77 At1g35550.1 68414.m04414 elongation factor Tu C-terminal domain-... 141 5e-34 At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 94 1e-19 At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 78 7e-15 At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 52 5e-07 At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 45 6e-05 At4g27900.2 68417.m04005 expressed protein 32 0.44 At4g27900.1 68417.m04004 expressed protein 32 0.44 At4g29900.1 68417.m04254 calcium-transporting ATPase, plasma mem... 29 4.1 At1g27900.1 68414.m03419 RNA helicase, putative similar to SP|Q1... 29 4.1 At4g25650.1 68417.m03693 Rieske [2Fe-2S] domain-containing prote... 28 5.4 At4g18180.1 68417.m02701 glycoside hydrolase family 28 protein /... 28 5.4 At4g01910.1 68417.m00251 DC1 domain-containing protein contains ... 27 9.5 >At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha identical to SWISS-PROT:P13905 elongation factor 1-alpha (EF-1-alpha) [Arabidopsis thaliana] Length = 449 Score = 286 bits (701), Expect = 1e-77 Identities = 126/179 (70%), Positives = 157/179 (87%) Frame = +2 Query: 41 IGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRG 220 +GRVETG++KPG +V FAP +TTEVKSVEMHHE+L EA+PGDNVGFNVKNV+VK+L+RG Sbjct: 252 VGRVETGMIKPGMVVTFAPTGLTTEVKSVEMHHESLLEALPGDNVGFNVKNVAVKDLKRG 311 Query: 221 YVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRT 400 YVA +SK++P KGAA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+EI K+DRR+ Sbjct: 312 YVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRS 371 Query: 401 GKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVN 577 GK E PK +K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V+ Sbjct: 372 GKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVD 430 >At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha identical to GB:CAA34456 from [Arabidopsis thaliana] (Plant Mol. Biol. 14 (1), 107-110 (1990)) Length = 449 Score = 286 bits (701), Expect = 1e-77 Identities = 126/179 (70%), Positives = 157/179 (87%) Frame = +2 Query: 41 IGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRG 220 +GRVETG++KPG +V FAP +TTEVKSVEMHHE+L EA+PGDNVGFNVKNV+VK+L+RG Sbjct: 252 VGRVETGMIKPGMVVTFAPTGLTTEVKSVEMHHESLLEALPGDNVGFNVKNVAVKDLKRG 311 Query: 221 YVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRT 400 YVA +SK++P KGAA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+EI K+DRR+ Sbjct: 312 YVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRS 371 Query: 401 GKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVN 577 GK E PK +K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V+ Sbjct: 372 GKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVD 430 >At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha identical to GB:CAA34456 from [Arabidopsis thaliana] (Plant Mol. Biol. 14 (1), 107-110 (1990)) Length = 449 Score = 286 bits (701), Expect = 1e-77 Identities = 126/179 (70%), Positives = 157/179 (87%) Frame = +2 Query: 41 IGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRG 220 +GRVETG++KPG +V FAP +TTEVKSVEMHHE+L EA+PGDNVGFNVKNV+VK+L+RG Sbjct: 252 VGRVETGMIKPGMVVTFAPTGLTTEVKSVEMHHESLLEALPGDNVGFNVKNVAVKDLKRG 311 Query: 221 YVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRT 400 YVA +SK++P KGAA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+EI K+DRR+ Sbjct: 312 YVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRS 371 Query: 401 GKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVN 577 GK E PK +K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V+ Sbjct: 372 GKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVD 430 >At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha identical to GB:CAA34456 from [Arabidopsis thaliana] (Plant Mol. Biol. 14 (1), 107-110 (1990)) Length = 449 Score = 286 bits (701), Expect = 1e-77 Identities = 126/179 (70%), Positives = 157/179 (87%) Frame = +2 Query: 41 IGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRG 220 +GRVETG++KPG +V FAP +TTEVKSVEMHHE+L EA+PGDNVGFNVKNV+VK+L+RG Sbjct: 252 VGRVETGMIKPGMVVTFAPTGLTTEVKSVEMHHESLLEALPGDNVGFNVKNVAVKDLKRG 311 Query: 221 YVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRT 400 YVA +SK++P KGAA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+EI K+DRR+ Sbjct: 312 YVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRS 371 Query: 401 GKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVN 577 GK E PK +K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V+ Sbjct: 372 GKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVD 430 >At1g35550.1 68414.m04414 elongation factor Tu C-terminal domain-containing protein similar to SP|P13905 Elongation factor 1-alpha (EF-1-alpha) {Arabidopsis thaliana}; contains Pfam profile PF03143: Elongation factor Tu C-terminal domain Length = 104 Score = 141 bits (341), Expect = 5e-34 Identities = 61/95 (64%), Positives = 77/95 (81%) Frame = +2 Query: 290 LNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVP 469 +NH GQI NGYTPVLDCHT+HIA KF+EI K+D RTG E PK +K+ +AAI+N+ P Sbjct: 1 MNHLGQIKNGYTPVLDCHTSHIAVKFSEILTKIDWRTGHEIEKEPKFLKNSEAAIINMTP 60 Query: 470 SKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 574 +KP+ VE++ +PPLGRFA+RDMRQTV VGVIK+V Sbjct: 61 TKPMVVEAYSAYPPLGRFAIRDMRQTVGVGVIKSV 95 >At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein, putative similar to EF-1-alpha-related GTP-binding protein gi|1009232|gb|AAA79032 Length = 532 Score = 93.9 bits (223), Expect = 1e-19 Identities = 48/177 (27%), Positives = 91/177 (51%), Gaps = 2/177 (1%) Frame = +2 Query: 41 IGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRG 220 +G+VE+G ++ G +V P +V ++ + ++ A PG+N+ + + +++ G Sbjct: 347 MGKVESGSIREGDSLVVMPNKEQVKVVAIYCDEDKVKRAGPGENLRVRITGIEDEDILSG 406 Query: 221 YVAGDSKNNPPKGAADFTAQVIVLN--HPGQISNGYTPVLDCHTAHIACKFAEIKEKVDR 394 +V S NP +F AQ+ +L + GY +L H C+ E+K ++D Sbjct: 407 FVLS-SIVNPVPAVTEFVAQLQILELLDNAIFTAGYKAILHIHAVVEECEIIELKSQIDL 465 Query: 395 RTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVI 565 +T K + +K+G A + + + +C+E F +FP LGRF +R +T+AVG + Sbjct: 466 KTRKPMKKKVLFVKNGAAVVCRIQVTNSICIEKFSDFPQLGRFTLRTEGKTIAVGKV 522 >At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / EF-1-alpha, putative contains similarity to SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha) [Aeropyrum pernix] Length = 667 Score = 77.8 bits (183), Expect = 7e-15 Identities = 47/177 (26%), Positives = 86/177 (48%) Frame = +2 Query: 44 GRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGY 223 G++E G ++PG+ V+ P+ ++S+E +A A GDNV ++ + ++ G Sbjct: 488 GKLEAGAVRPGSKVMVMPSGDQGTIRSLERDSQACTIARAGDNVALALQGIDANQVMAGD 547 Query: 224 VAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTG 403 V + P A V+VL I G H A A ++ +D +TG Sbjct: 548 VLCHP-DFPVSVATHLELMVLVLEGATPILLGSQLEFHVHHAKEAATVVKLVAMLDPKTG 606 Query: 404 KSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 574 + T+ +P+ + + +A++ + P+CVE+F E LGR +R +TVA+G + + Sbjct: 607 QPTKKSPRCLTAKQSAMLEVSLQNPVCVETFSESRALGRVFLRSSGRTVAMGKVTRI 663 >At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) identical to SWISS-PROT:P17745 elongation factor Tu, chloroplast precursor (EF-Tu) [Arabidopsis thaliana] Length = 476 Score = 51.6 bits (118), Expect = 5e-07 Identities = 49/182 (26%), Positives = 73/182 (40%), Gaps = 5/182 (2%) Frame = +2 Query: 44 GRVETGVLKPGTIVVFAPANITTE--VKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRR 217 GRVE G +K G V T V VEM + L EA+ GDNVG ++ + +++R Sbjct: 307 GRVERGTVKVGETVDLVGLRETRSYTVTGVEMFQKILDEALAGDNVGLLLRGIQKADIQR 366 Query: 218 GYVAGDSKNNPPKGAADFTAQVIVLNHPGQIS---NGYTPVLDCHTAHIACKFAEIKEKV 388 G V + P + V+ G+ S GY P T + K +I Sbjct: 367 GMVLAKPGSITPHTKFEAIIYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVTKIMNDK 426 Query: 389 DRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 568 D + K + GD + + P+ E RFA+R+ +TV GVI Sbjct: 427 DEES--------KMVMPGDRVKIVVELIVPVACEQGM------RFAIREGGKTVGAGVIG 472 Query: 569 AV 574 + Sbjct: 473 TI 474 >At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu, putative similar to mitochondrial elongation factor Tu [Arabidopsis thaliana] gi|1149571|emb|CAA61511 Length = 454 Score = 44.8 bits (101), Expect = 6e-05 Identities = 51/184 (27%), Positives = 79/184 (42%), Gaps = 7/184 (3%) Frame = +2 Query: 44 GRVETGVLKPGTIVVFAPAN-----ITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKE 208 GR+E GV+K G V + + V VEM + L GDNVG ++ + ++ Sbjct: 287 GRIEQGVIKVGEEVEILGLREGGVPLKSTVTGVEMFKKILDNGQAGDNVGLLLRGLKRED 346 Query: 209 LRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKV 388 ++RG V +K K F A++ VL +T + + A+I KV Sbjct: 347 IQRGMVI--AKPGSCKTYKKFEAEIYVLTK--DEGGRHTAFFSNYRPQFYLRTADITGKV 402 Query: 389 DRRTGKSTEVNPKSIKSGD--AAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGV 562 + N K + GD A+ L+ PL E+ Q RFA+R+ +TV GV Sbjct: 403 E------LPENVKMVMPGDNVTAVFELIMPVPL--ETGQ------RFALREGGRTVGAGV 448 Query: 563 IKAV 574 + V Sbjct: 449 VSKV 452 >At4g27900.2 68417.m04005 expressed protein Length = 261 Score = 31.9 bits (69), Expect = 0.44 Identities = 11/31 (35%), Positives = 21/31 (67%) Frame = +2 Query: 422 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 514 PKS+ SG+ + +++V K ++ F +FPP+ Sbjct: 144 PKSVSSGNLSSMDMVEHKDAVIQGFPDFPPV 174 >At4g27900.1 68417.m04004 expressed protein Length = 261 Score = 31.9 bits (69), Expect = 0.44 Identities = 11/31 (35%), Positives = 21/31 (67%) Frame = +2 Query: 422 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 514 PKS+ SG+ + +++V K ++ F +FPP+ Sbjct: 144 PKSVSSGNLSSMDMVEHKDAVIQGFPDFPPV 174 >At4g29900.1 68417.m04254 calcium-transporting ATPase, plasma membrane-type, putative / Ca2+-ATPase, putative (ACA10) identical to SP|Q9SZR1 Potential calcium-transporting ATPase 10, plasma membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform 10) {Arabidopsis thaliana}; similar to SP|Q9LF79 Calcium-transporting ATPase 8, plasma membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform 8) {Arabidopsis thaliana} Length = 1069 Score = 28.7 bits (61), Expect = 4.1 Identities = 11/34 (32%), Positives = 18/34 (52%) Frame = -3 Query: 162 GTASWRASWCISTDLTSVVMLAGAKTTMVPGFNT 61 G + WR W S DLT ++++ A ++ G T Sbjct: 178 GRSFWRFVWEASQDLTLIILIVAAVASLALGIKT 211 >At1g27900.1 68414.m03419 RNA helicase, putative similar to SP|Q14562 ATP-dependent helicase DDX8 (RNA helicase HRH1) (DEAH-box protein 8) {Homo sapiens}; contains Pfam profiles PF04408: Helicase associated domain (HA2), PF00271: Helicase conserved C-terminal domain Length = 700 Score = 28.7 bits (61), Expect = 4.1 Identities = 16/42 (38%), Positives = 25/42 (59%) Frame = -3 Query: 183 LKPTLSPGTASWRASWCISTDLTSVVMLAGAKTTMVPGFNTP 58 L+P+LS + C+S LT V ML+ A+TT++P + P Sbjct: 428 LEPSLSRTLIEANETGCLSQALTVVAMLS-AETTLLPARSKP 468 >At4g25650.1 68417.m03693 Rieske [2Fe-2S] domain-containing protein similar to cell death suppressor protein lls1 from Zea mays [gi:1935909], Rieske iron-sulfur protein Tic55 from Pisum sativum [gi:2764524]; contains Pfam PF00355 Rieske [2Fe-2S] domain Length = 536 Score = 28.3 bits (60), Expect = 5.4 Identities = 11/24 (45%), Positives = 16/24 (66%) Frame = +2 Query: 362 KFAEIKEKVDRRTGKSTEVNPKSI 433 +F + KEK+DR GK E+N K + Sbjct: 258 RFPKPKEKIDREGGKPLEINVKKL 281 >At4g18180.1 68417.m02701 glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein similar to polygalacturonase [Salix gilgiana] GI:6714524; contains PF00295: Glycosyl hydrolases family 28 (polygalacturonases) Length = 414 Score = 28.3 bits (60), Expect = 5.4 Identities = 21/71 (29%), Positives = 33/71 (46%) Frame = -2 Query: 424 WVDFSRFTSTTVNFFFDFCKFAGNVGSVAIQYWCVTV*DLTRMVKHNDLSCKICSTLRWV 245 W + +S++VN +F+ VGS+ C V +LT VK + K S W+ Sbjct: 58 WDKACKSSSSSVNLIIPRGEFS--VGSLRFSGPCTNVSNLTVRVKASTDLSKYRSGGGWI 115 Query: 244 VFGVTSNITTT 212 FG + +T T Sbjct: 116 QFGWINGLTLT 126 >At4g01910.1 68417.m00251 DC1 domain-containing protein contains Pfam profile PF03107: DC1 domain Length = 651 Score = 27.5 bits (58), Expect = 9.5 Identities = 10/22 (45%), Positives = 13/22 (59%) Frame = +1 Query: 61 CVETRYHCCLCPRQHHY*SQIC 126 C R+HC LC R+ +Y S C Sbjct: 73 CSNLRFHCDLCGRRTNYLSYYC 94 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,873,100 Number of Sequences: 28952 Number of extensions: 339466 Number of successful extensions: 992 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 952 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 988 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1565336320 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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