BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30649 (810 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 71 2e-13 SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 71 2e-13 SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 57 3e-09 SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 31 0.15 SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 27 3.2 SPBC215.06c |||human LYHRT homolog|Schizosaccharomyces pombe|chr... 27 4.2 SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 26 7.3 SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces pombe... 25 9.6 >SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 70.9 bits (166), Expect = 2e-13 Identities = 47/151 (31%), Positives = 76/151 (50%), Gaps = 3/151 (1%) Frame = +2 Query: 35 MSDLSKNDVERASF--AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXX 208 M+ + D + A F AFS++D + G I + LG ++R+L +PT A + Sbjct: 1 MTTRNLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDAD 60 Query: 209 XXXXXXXX-FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKL 385 FL + ++ KD D E+ E K++DK+ NG + ELTH L +LGE+L Sbjct: 61 GNGTIDFTEFLTMMARKMKDTDNE--EEVREAFKVFDKDGNGYITVEELTHVLTSLGERL 118 Query: 386 DDSEVAEVTKDCMDPEDDDGMIPYAAFLKKV 478 EVA++ ++ D DG+I Y F + + Sbjct: 119 SQEEVADMIREA--DTDGDGVINYEEFSRVI 147 >SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pombe|chr 1|||Manual Length = 141 Score = 70.9 bits (166), Expect = 2e-13 Identities = 44/136 (32%), Positives = 71/136 (52%) Frame = +2 Query: 77 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXXFLPIYSQA 256 AFS++D G G+I ++GDLLRA NPTLA I FL + ++ Sbjct: 11 AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI---TEIESTLPAEVDMEQFLQVLNRP 67 Query: 257 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPED 436 G E+F++ +++DK+ G++ EL + L +LGEKL + E+ E+ K Sbjct: 68 NGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PV 124 Query: 437 DDGMIPYAAFLKKVMA 484 DGM+ Y F++ ++A Sbjct: 125 KDGMVNYHDFVQMILA 140 >SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces pombe|chr 1|||Manual Length = 143 Score = 57.2 bits (132), Expect = 3e-09 Identities = 37/146 (25%), Positives = 71/146 (48%) Frame = +2 Query: 47 SKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXX 226 SK + AF +YD + G I ++G +LR+L N T A + Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63 Query: 227 XXFLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAE 406 F+ + K ++ + E++++ +++DK+ +G + A+ + LGEKL D+EV Sbjct: 64 MSFV-----SNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQL 118 Query: 407 VTKDCMDPEDDDGMIPYAAFLKKVMA 484 + ++ DP + G Y F++++MA Sbjct: 119 MVQEA-DP-TNSGSFDYYDFVQRIMA 142 >SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 31.5 bits (68), Expect = 0.15 Identities = 32/161 (19%), Positives = 63/161 (39%), Gaps = 1/161 (0%) Frame = +2 Query: 2 KTGGNRQTPHKMSDLSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIX 181 + GG ++++ + D+ A F ++D + ID L +RAL N + + Sbjct: 20 RLGGYAPLRVEITEEQRQDINEA---FKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVL 76 Query: 182 XXXXXXXXXXXXXXXXX-FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTH 358 F+ + ++ ++D E+ +L+D +E G + L Sbjct: 77 KILRDFDKTGKGYLQMEDFVRVMTEKIVERDP--LEEIKRAFELFDDDETGKISLRNLRR 134 Query: 359 TLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKKVM 481 L E +DD E+ + ++ D DG I F+ +M Sbjct: 135 VAKELNENIDDQELEAMIEEF--DLDQDGEINEQEFIAIMM 173 >SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 834 Score = 27.1 bits (57), Expect = 3.2 Identities = 13/29 (44%), Positives = 18/29 (62%), Gaps = 1/29 (3%) Frame = +2 Query: 35 MSDLSKNDVER-ASFAFSIYDFEGKGKID 118 +++L DV R SF F +YDF G G +D Sbjct: 605 IAELKFRDVMRNISFIFELYDFNGDGFMD 633 >SPBC215.06c |||human LYHRT homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 178 Score = 26.6 bits (56), Expect = 4.2 Identities = 19/92 (20%), Positives = 41/92 (44%), Gaps = 1/92 (1%) Frame = +3 Query: 21 KHHTK*ATSARTTLKGRLSPSQSTTLKAKVR-SMPSTLAIS*ERSTQTPHWQPSRNSVVQ 197 ++HT T A+ KG P++ KAK+ + ++ +S Q P+++S+ + Sbjct: 43 RNHTSCMTEAQRYQKGLYRPTKKELKKAKMNGNAVNSKELSPNTDNQNTPAGPTKHSLDE 102 Query: 198 RRRARSCSHSKSSFPSTAKQRKTKTRERMKIS 293 + + SK S+ ++ + +IS Sbjct: 103 NEKDKENKKSKKETVSSPAEQLLALTQNQEIS 134 >SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces pombe|chr 2|||Manual Length = 1142 Score = 25.8 bits (54), Expect = 7.3 Identities = 9/22 (40%), Positives = 14/22 (63%) Frame = +2 Query: 371 LGEKLDDSEVAEVTKDCMDPED 436 L EK+ D + + DC+DP+D Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798 >SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces pombe|chr 3|||Manual Length = 551 Score = 25.4 bits (53), Expect = 9.6 Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 2/75 (2%) Frame = +3 Query: 54 TTLKGRLSPSQSTTLKA-KVRSMPS-TLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSHS 227 T +PS L+A + S+P+ +L S E + + +R ++ ++ S + Sbjct: 309 TEYSSNTAPSNWIALEALPLVSIPNESLDESDELAESLSDSEAARLQLLGIKKQESVAKK 368 Query: 228 KSSFPSTAKQRKTKT 272 KSSFPST K + T Sbjct: 369 KSSFPSTIKDQPNLT 383 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,067,007 Number of Sequences: 5004 Number of extensions: 57562 Number of successful extensions: 169 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 158 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 164 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 394431430 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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