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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30648
         (692 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||...   395   e-111
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb...   223   2e-59
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s...   213   3e-56
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce...   162   4e-41
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe...   116   4e-27
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy...   113   3e-26
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc...   103   2e-23
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa...    67   3e-12
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po...    54   2e-08
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb...    38   0.002
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr...    31   0.16 
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce...    30   0.28 
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p...    26   4.5  
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom...    26   4.5  
SPCC970.03 |||cytochrome b5 reductase |Schizosaccharomyces pombe...    26   5.9  

>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 375

 Score =  395 bits (972), Expect = e-111
 Identities = 179/196 (91%), Positives = 191/196 (97%)
 Frame = +2

Query: 62  DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 241
           ++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK
Sbjct: 2   EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61

Query: 242 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 421
           RGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQ
Sbjct: 62  RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121

Query: 422 IMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLA 601
           I+FETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIYEGYALPHAI+RLDLA
Sbjct: 122 IIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRLDLA 181

Query: 602 GRDLTDYLMKILTERG 649
           GRDLTDYLMKIL ERG
Sbjct: 182 GRDLTDYLMKILMERG 197



 Score = 31.9 bits (69), Expect = 0.090
 Identities = 13/15 (86%), Positives = 15/15 (100%)
 Frame = +1

Query: 646 GYSFTTTAEREIVRD 690
           GY+F+TTAEREIVRD
Sbjct: 197 GYTFSTTAEREIVRD 211


>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 379

 Score =  223 bits (545), Expect = 2e-59
 Identities = 102/195 (52%), Positives = 141/195 (72%), Gaps = 1/195 (0%)
 Frame = +2

Query: 68  DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 247
           D   + +DNGSG  KAGFAGDD P+ +FP+ VGR +H+ VM    QKD +VG EAQ+ RG
Sbjct: 8   DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67

Query: 248 ILTLKYPIEHGIITNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTQI 424
           +L ++ PIE GII NW DME+IW + + ++ L   PEEHP+LLTE PL    N+EK+ + 
Sbjct: 68  LLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEY 127

Query: 425 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAG 604
            +ET N PA+  ++Q VL+LYAS RTTGIVL+ GDG++H+VPIY+G+++P AI + ++ G
Sbjct: 128 FYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAIQQEEIGG 187

Query: 605 RDLTDYLMKILTERG 649
           RD+TDYL   L + G
Sbjct: 188 RDVTDYLQLQLRKSG 202


>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
           subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 390

 Score =  213 bits (519), Expect = 3e-56
 Identities = 99/194 (51%), Positives = 139/194 (71%), Gaps = 2/194 (1%)
 Frame = +2

Query: 74  AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRG 247
           A +V+DNG+G  K G+A D+ PR  FPSIVGRP  R +     +  KD  VGDEA++ R 
Sbjct: 4   APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63

Query: 248 ILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIM 427
           +L +KYP+E+GII ++++M ++W +TF+ +L++ P    +LLTE P+NP ANREKM + M
Sbjct: 64  LLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETM 123

Query: 428 FETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGR 607
           FE +    +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE   L H + RLD+AGR
Sbjct: 124 FERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVGRLDVAGR 183

Query: 608 DLTDYLMKILTERG 649
           D T YL+ +L  +G
Sbjct: 184 DATRYLISLLLRKG 197


>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 427

 Score =  162 bits (394), Expect = 4e-41
 Identities = 90/221 (40%), Positives = 127/221 (57%), Gaps = 32/221 (14%)
 Frame = +2

Query: 80  LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 208
           +++DNG+G  K G+AG+DAP  VFP+++   R  G   G         M  K        
Sbjct: 8   IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66

Query: 209 ------DSYVGDEAQSKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 367
                 D ++G++A  K     +L YPI HG I NWD ME+ W  + +  LR  PE+H  
Sbjct: 67  RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126

Query: 368 LLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRT--------TGIVLDS 523
           LLTE PLNP  NRE   +IMFE+FN   +Y+A+QAVL+L AS  +        TG V+DS
Sbjct: 127 LLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDS 186

Query: 524 GDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTER 646
           GDGV+H +P+ EGY +  +I  + LAGRD+T ++  +L +R
Sbjct: 187 GDGVTHIIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDR 227


>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 721

 Score =  116 bits (278), Expect = 4e-27
 Identities = 66/214 (30%), Positives = 119/214 (55%), Gaps = 3/214 (1%)
 Frame = +2

Query: 2   AAVESIPASPTQTHTKFKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQ 181
           A  E + + PT +   F+   +D+  LV+DNGS   +AG+ G+  P+ VF ++V R R +
Sbjct: 5   AVREPVFSGPTPS---FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDR 60

Query: 182 GVMVGMGQKDSYVGDEAQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEE 358
                + +  + VG++   + G  ++ + P E  +I+NWD ME++  +TF  +L +   E
Sbjct: 61  K----LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRME 115

Query: 359 HPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGR--TTGIVLDSGDG 532
           HP+ +TE   NP   R  MT+++FE +N+P++   I  + S Y + +  ++GIVL+ G+ 
Sbjct: 116 HPICMTEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNA 175

Query: 533 VSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKI 634
            SH +P+  G  +     R+   G   + YL+K+
Sbjct: 176 ASHVIPVLNGERILSEAKRISWGGSQSSSYLLKL 209


>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 433

 Score =  113 bits (271), Expect = 3e-26
 Identities = 66/206 (32%), Positives = 103/206 (50%)
 Frame = +2

Query: 65  DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 244
           D+V+A+V+D GS   + GF+G+D P+ V PS  G       + G    + Y+    +S  
Sbjct: 9   DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNP 61

Query: 245 GILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQI 424
           G + +K  I +G + NWD    +W +    +L+  P EHP+L+TE   NP  NR K  + 
Sbjct: 62  G-MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLET 120

Query: 425 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAG 604
           MFE+   PA Y+A Q   + +ASG+ T  ++D G   S    IY+G+ L         +G
Sbjct: 121 MFESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVLQKGYQVQHFSG 180

Query: 605 RDLTDYLMKILTERGTRSPPPLRGKS 682
             + D L + L ++     P    KS
Sbjct: 181 NAINDILAQTLRDKNFEVMPKYLVKS 206


>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
           Arp42|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 430

 Score =  103 bits (247), Expect = 2e-23
 Identities = 56/205 (27%), Positives = 105/205 (51%), Gaps = 2/205 (0%)
 Frame = +2

Query: 65  DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 244
           +++ +LV+D GS   + G+AG+++P  + PS      + GV   +  ++ YV DE Q   
Sbjct: 8   EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61

Query: 245 GI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 418
            I  + +K    +GII +W+     W      +L+V P E+ +++TE   NP++ R+++ 
Sbjct: 62  PIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIM 121

Query: 419 QIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDL 598
           +  FE  + PA Y+  QAV   +A+ ++T +++D G   +   P+ +G  +   I +  L
Sbjct: 122 EAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIGSDNASVTPVVDGLIIRKGIFKQSL 181

Query: 599 AGRDLTDYLMKILTERGTRSPPPLR 673
           AG  L   + ++        PP  R
Sbjct: 182 AGDFLNANIEQLFNTMNIEFPPHYR 206


>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
           Arp9|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 523

 Score = 66.9 bits (156), Expect = 3e-12
 Identities = 40/130 (30%), Positives = 63/130 (48%), Gaps = 4/130 (3%)
 Frame = +2

Query: 266 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 433
           PI+ G + +W+ ++  W H  Y+ L   P +    +PV L         +RE  TQ  FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171

Query: 434 TFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDL 613
               P   +A + ++ LYA G   G+V+D G   +   PI +G  +  A  +L L GR +
Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLGGRYM 231

Query: 614 TDYLMKILTE 643
           T +L  +L E
Sbjct: 232 TQHLQNLLRE 241


>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 401

 Score = 54.0 bits (124), Expect = 2e-08
 Identities = 50/207 (24%), Positives = 90/207 (43%), Gaps = 18/207 (8%)
 Frame = +2

Query: 80  LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 259
           +V+DNG+   KAGFAG        P+ + R +        G +  ++G+E  +     TL
Sbjct: 8   IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSKD-------GNR-LFLGNELANCNDFTTL 57

Query: 260 KYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEAPLNPKANREKMTQIM 427
           ++   H  G + +W     +W     N   + P   ++ +LLT+      +      Q++
Sbjct: 58  QFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQPVFTMPSIEHNTIQLV 117

Query: 428 FETFNSPAMYVAIQAVLSLYASGRTT--------------GIVLDSGDGVSHTVPIYEGY 565
           FE F   A      A L  +  G  T               +V+DSG   +H +P+ +  
Sbjct: 118 FEEFQFDAYLPCTPAELIPWDHGSFTMNQEDAYTGQHGECVLVIDSGYSFTHIIPVIDFS 177

Query: 566 ALPHAILRLDLAGRDLTDYLMKILTER 646
               A+ R+D+ G+ LT+YL ++++ R
Sbjct: 178 VQEQAVKRVDVGGKLLTNYLKEVISYR 204


>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 620

 Score = 37.5 bits (83), Expect = 0.002
 Identities = 28/139 (20%), Positives = 65/139 (46%), Gaps = 12/139 (8%)
 Frame = +2

Query: 257 LKYPIEHGIITNWD---------DMEKIWHHTFYNELRVAPEE---HPVLLTEAPLNPKA 400
           L YPI+ G+    D         D+ +I+ ++  + L++   +   + V+     L  + 
Sbjct: 198 LFYPIKSGVFNESDYASSQQLLADIYEIFKYSITSLLQIPVSQLSQYSVIFIVPDLYDRV 257

Query: 401 NREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHA 580
             EK+  I+F   +     +  +++ + + +G +   V+D G   +    + EG  +P++
Sbjct: 258 YVEKILDILFFDLHFGKAAIVQESLCTSFGAGMSAACVVDMGAQKTSISCVEEGVVVPNS 317

Query: 581 ILRLDLAGRDLTDYLMKIL 637
            ++++  G D+T   MK+L
Sbjct: 318 RIKINYGGDDITLLFMKLL 336


>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1517

 Score = 31.1 bits (67), Expect = 0.16
 Identities = 16/48 (33%), Positives = 26/48 (54%)
 Frame = +2

Query: 125 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 268
           G+  PRA F  ++  P H G+++ M  KD   G+E  S +G  + + P
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547


>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1666

 Score = 30.3 bits (65), Expect = 0.28
 Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
 Frame = +2

Query: 326  FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 469
            +Y  L    E+HP+LLT+  A L P+ +  ++ +I  ++ N+P +    VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451


>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 226

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 10/25 (40%), Positives = 18/25 (72%)
 Frame = +2

Query: 368 LLTEAPLNPKANREKMTQIMFETFN 442
           +L  APL+ +   +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205


>SPAC1296.04 |mug65||spore wall assembly protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 248

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 9/16 (56%), Positives = 9/16 (56%)
 Frame = +3

Query: 552 STKVMLCPTPSSVWTW 599
           STK   CP PS  W W
Sbjct: 52  STKTATCPDPSWEWAW 67


>SPCC970.03 |||cytochrome b5 reductase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 301

 Score = 25.8 bits (54), Expect = 5.9
 Identities = 10/16 (62%), Positives = 12/16 (75%), Gaps = 1/16 (6%)
 Frame = +3

Query: 537 PTPYPSTKVMLC-PTP 581
           P P P TKV++C PTP
Sbjct: 256 PAPSPETKVLICGPTP 271


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,744,838
Number of Sequences: 5004
Number of extensions: 56027
Number of successful extensions: 208
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 199
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 321951680
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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