BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30645 (626 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein... 78 9e-16 SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 65 7e-12 SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ... 38 9e-04 SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase Ubp4|Schizos... 27 2.9 SPBC354.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 3.9 SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 26 3.9 SPCC16C4.18c |taf50||histone H4-like TAF |Schizosaccharomyces po... 26 3.9 SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32 |Schi... 26 5.1 SPCC1795.11 |sum3|ded1, slh3, moc2|ATP-dependent RNA helicase Su... 26 5.1 SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein Sad1|S... 26 5.1 SPAC637.09 |||ribonuclease H70 |Schizosaccharomyces pombe|chr 1|... 25 6.8 SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 25 9.0 SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1 |Schizosaccharom... 25 9.0 SPAC31A2.16 |gef2||RhoGEF Gef2|Schizosaccharomyces pombe|chr 1||... 25 9.0 SPAC2G11.05c |||BRO1 domain protein|Schizosaccharomyces pombe|ch... 25 9.0 SPCC550.09 |||peroxin Pex32 |Schizosaccharomyces pombe|chr 3|||M... 25 9.0 SPAC19E9.03 |pas1|SPAC57A10.01|cyclin Pas1|Schizosaccharomyces p... 25 9.0 >SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein Stg1|Schizosaccharomyces pombe|chr 1|||Manual Length = 174 Score = 78.2 bits (184), Expect = 9e-16 Identities = 55/146 (37%), Positives = 77/146 (52%), Gaps = 4/146 (2%) Frame = +3 Query: 75 EQEVLTWISDVLGEPLPNGAYE--DVLRDGVILCKLANKLAPGSVKKIQERGTNFQLMEN 248 E+E WI + L L N + D L+ GVILC++ K A G+ + +E F MEN Sbjct: 6 EKEAREWIEETLHTKL-NAQLDLLDQLQSGVILCRIC-KEALGANIRYKESNMPFVQMEN 63 Query: 249 IQRF-QAAIKKYGVPEEEIFQTADLFERRNIPQVTLCLYALGRITQKHPEFTGPQLGPKM 425 I F A + VP +++FQT+DLFERRN QV +++ R K LGPK+ Sbjct: 64 ISAFINYAQQVVHVPSQDMFQTSDLFERRNDEQVLRSIHSFSRYAAKMFPGKVRGLGPKL 123 Query: 426 ADKNERTFTEEQLRAHNAELN-LQMG 500 A+K R F+ +Q R +N LQ G Sbjct: 124 AEKKPRVFSAQQQREFREGVNSLQYG 149 >SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual Length = 1489 Score = 65.3 bits (152), Expect = 7e-12 Identities = 35/98 (35%), Positives = 52/98 (53%), Gaps = 2/98 (2%) Frame = +3 Query: 81 EVLTWISDVLGEPL-PNGAYEDVLRDGVILCKLANKLAPGSVKKI-QERGTNFQLMENIQ 254 E WI + LG L P +E LR+GV+L L K P + KI F+ +NI Sbjct: 46 EAKKWIEECLGTDLGPTSTFEQSLRNGVVLALLVQKFQPDKLIKIFYSNELQFRHSDNIN 105 Query: 255 RFQAAIKKYGVPEEEIFQTADLFERRNIPQVTLCLYAL 368 +F I G+PE F+ D++E +N+P+V C++AL Sbjct: 106 KFLDFIHGIGLPEIFHFELTDIYEGKNLPKVIYCIHAL 143 >SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr 2|||Manual Length = 962 Score = 38.3 bits (85), Expect = 9e-04 Identities = 30/101 (29%), Positives = 45/101 (44%), Gaps = 5/101 (4%) Frame = +3 Query: 81 EVLTWISDVLGEPLPN-GAYEDVLRDGVILCKLANKLAPGSVKKIQERGTNFQLMEN--- 248 E W+ + N + D L +G +LC+LA K P + R +Q+ E Sbjct: 69 EAKKWLEEETNNEYQNLDDFVDALVNGKVLCQLAFKYYPKLASNWKPR---YQISERNTV 125 Query: 249 -IQRFQAAIKKYGVPEEEIFQTADLFERRNIPQVTLCLYAL 368 + F + G+ F+T DL R NIP+V CL+AL Sbjct: 126 YLNAFFHFLDFIGMFTPFRFETKDLVRRFNIPKVIYCLHAL 166 >SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase Ubp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 438 Score = 26.6 bits (56), Expect = 2.9 Identities = 14/41 (34%), Positives = 22/41 (53%) Frame = -2 Query: 244 SMSWKLVPLSWIFFTDPGANLLANLQRITPSLSTSSYAPFG 122 S+++KL + DP LAN +++ SLS +Y P G Sbjct: 33 SLAYKLYSMLRSLKLDPHFMELANPKKVDSSLSYENYQPIG 73 >SPBC354.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 163 Score = 26.2 bits (55), Expect = 3.9 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = +2 Query: 161 DPLQIGQQISSWISEKNPRERNQLPAHG 244 D LQ+ ++ SWI+EK E +L + G Sbjct: 11 DSLQVDEKAKSWIAEKVEEELRRLESVG 38 >SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces pombe|chr 1|||Manual Length = 1957 Score = 26.2 bits (55), Expect = 3.9 Identities = 10/19 (52%), Positives = 14/19 (73%) Frame = -2 Query: 613 FTNTDINVENIGKNLEAIY 557 F N VEN+GK++EA+Y Sbjct: 1855 FDNYSEKVENLGKSIEALY 1873 >SPCC16C4.18c |taf50||histone H4-like TAF |Schizosaccharomyces pombe|chr 3|||Manual Length = 452 Score = 26.2 bits (55), Expect = 3.9 Identities = 17/64 (26%), Positives = 27/64 (42%) Frame = -2 Query: 229 LVPLSWIFFTDPGANLLANLQRITPSLSTSSYAPFGSGSPKTSEIQVRTSCSWSLFLAGI 50 L+ ++W +P + +Q++ PS+ T A P E + FL GI Sbjct: 257 LMHMAWALLDNPNLFVEPYVQQLMPSILTCLVAKRLGSDPNNHEHYALRDL--AAFLLGI 314 Query: 49 VCRR 38 VC R Sbjct: 315 VCDR 318 >SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32 |Schizosaccharomyces pombe|chr 2|||Manual Length = 749 Score = 25.8 bits (54), Expect = 5.1 Identities = 11/30 (36%), Positives = 15/30 (50%) Frame = -2 Query: 181 LANLQRITPSLSTSSYAPFGSGSPKTSEIQ 92 L+N +TPS S S Y P +T +Q Sbjct: 689 LSNFNSVTPSSSASLYPPLIPSEARTPSVQ 718 >SPCC1795.11 |sum3|ded1, slh3, moc2|ATP-dependent RNA helicase Sum3|Schizosaccharomyces pombe|chr 3|||Manual Length = 636 Score = 25.8 bits (54), Expect = 5.1 Identities = 14/52 (26%), Positives = 21/52 (40%) Frame = +3 Query: 384 KHPEFTGPQLGPKMADKNERTFTEEQLRAHNAELNLQMGFNKGASQSGHGGF 539 + F G G + + + R R N G++ G S SG+GGF Sbjct: 565 RESSFGGNGRGGRYSGRGGRGGNAYGARDFRRPTNSSSGYSSGPSYSGYGGF 616 >SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein Sad1|Schizosaccharomyces pombe|chr 2|||Manual Length = 514 Score = 25.8 bits (54), Expect = 5.1 Identities = 11/32 (34%), Positives = 18/32 (56%) Frame = +1 Query: 493 KWASIRAPPSPVMVASVTPATCKLLLNFSQYF 588 ++ IR+PP+ V + S+TP NF Q + Sbjct: 46 RYTKIRSPPTRVSIESITPKQRFPAPNFEQAY 77 >SPAC637.09 |||ribonuclease H70 |Schizosaccharomyces pombe|chr 1|||Manual Length = 623 Score = 25.4 bits (53), Expect = 6.8 Identities = 8/18 (44%), Positives = 11/18 (61%) Frame = -2 Query: 355 HNVTWGMLRLSKRSAVWN 302 HN WG R + +A+WN Sbjct: 491 HNFVWGRFRELEHAAMWN 508 >SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 780 Score = 25.0 bits (52), Expect = 9.0 Identities = 11/39 (28%), Positives = 23/39 (58%) Frame = -2 Query: 181 LANLQRITPSLSTSSYAPFGSGSPKTSEIQVRTSCSWSL 65 L++ I+ L ++ +PF S S +E++ RTS ++ + Sbjct: 26 LSSYTSISRELDYANQSPFSSNSFSPTELKARTSATFDV 64 >SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 787 Score = 25.0 bits (52), Expect = 9.0 Identities = 12/41 (29%), Positives = 21/41 (51%) Frame = +1 Query: 373 ELLKNIPSLQDLS*DLKWLIRTNVPSRKNNFGPTTLNLTSK 495 EL+ N PS D + L + + VP+ + FG + + +K Sbjct: 564 ELIPNDPSDDDYAAALNYAVNEYVPTYRKTFGNDSKKIKNK 604 >SPAC31A2.16 |gef2||RhoGEF Gef2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1101 Score = 25.0 bits (52), Expect = 9.0 Identities = 12/34 (35%), Positives = 20/34 (58%) Frame = +1 Query: 220 EEPTSSSWRTFKGSKRQSRNTEYQKKKYSKPPIF 321 E+P++S W+T GS+ ++ +KK S P F Sbjct: 185 EDPSNSYWKTVIGSREMFEDS--RKKTSSPSPSF 216 >SPAC2G11.05c |||BRO1 domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 701 Score = 25.0 bits (52), Expect = 9.0 Identities = 19/68 (27%), Positives = 34/68 (50%) Frame = -1 Query: 452 REGTFVLISHFRS*LRSCKLGMFLSNSSQSVQT*CYLGNVTPLKKIGGLEYFFFWYSVFL 273 +EGT L + ++S +++ +S V + N T +K +GGLE F + L Sbjct: 376 KEGTEKLFALMEE-IKSVNDNRYITETSNKV-----MENFTEIKNLGGLE-FLRSEASSL 428 Query: 272 DCRLEPLN 249 DC ++ +N Sbjct: 429 DCLIDKVN 436 >SPCC550.09 |||peroxin Pex32 |Schizosaccharomyces pombe|chr 3|||Manual Length = 535 Score = 25.0 bits (52), Expect = 9.0 Identities = 13/25 (52%), Positives = 15/25 (60%) Frame = -1 Query: 275 LDCRLEPLNVLHELEVGSSLLDFFH 201 LD LEPL LHE S+ L FF+ Sbjct: 238 LDAYLEPLVHLHEYSRRSNTLFFFY 262 >SPAC19E9.03 |pas1|SPAC57A10.01|cyclin Pas1|Schizosaccharomyces pombe|chr 1|||Manual Length = 411 Score = 25.0 bits (52), Expect = 9.0 Identities = 17/51 (33%), Positives = 25/51 (49%) Frame = +1 Query: 439 NVPSRKNNFGPTTLNLTSKWASIRAPPSPVMVASVTPATCKLLLNFSQYFP 591 N +RK + P T +S ++ AP +PV V S P+T +L Q P Sbjct: 212 NQATRKPSSSPQTTQDSSPILTM-APSTPVSVGSTPPSTPSVLPIAKQLAP 261 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,020,029 Number of Sequences: 5004 Number of extensions: 68846 Number of successful extensions: 240 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 229 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 239 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 277683324 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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