BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30642 (699 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g40610.1 68418.m04930 glycerol-3-phosphate dehydrogenase [NAD... 196 2e-50 At2g40690.1 68415.m05020 NAD-dependent glycerol-3-phosphate dehy... 83 1e-16 At5g63910.1 68418.m08025 expressed protein 33 0.24 At5g66690.1 68418.m08407 UDP-glucoronosyl/UDP-glucosyl transfera... 31 0.97 At3g50740.1 68416.m05552 UDP-glucoronosyl/UDP-glucosyl transfera... 31 0.97 At5g51490.1 68418.m06386 pectinesterase family protein contains ... 29 3.0 At2g29770.1 68415.m03617 kelch repeat-containing F-box family pr... 29 3.0 At2g17420.1 68415.m02010 thioredoxin reductase 2 / NADPH-depende... 29 3.0 At4g23460.1 68417.m03381 beta-adaptin, putative strong similarit... 28 6.8 At5g07800.1 68418.m00894 flavin-containing monooxygenase family ... 27 9.0 At4g39290.1 68417.m05564 kelch repeat-containing F-box family pr... 27 9.0 At2g26890.1 68415.m03226 DNAJ heat shock N-terminal domain-conta... 27 9.0 >At5g40610.1 68418.m04930 glycerol-3-phosphate dehydrogenase [NAD+] / GPDH strong similarity to SP|P52425 Glycerol-3-phosphate dehydrogenase [NAD+] (EC 1.1.1.8) {Cuphea lanceolata}; contains Pfam profile PF01210: NAD-dependent glycerol-3-phosphate dehydrogenase Length = 400 Score = 196 bits (477), Expect = 2e-50 Identities = 99/194 (51%), Positives = 131/194 (67%), Gaps = 2/194 (1%) Frame = +1 Query: 118 KNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEII-EGKKLTEIINETHENV 294 K+KV +VGSGNWGS AK++ NA L +F D V MWV+EE++ G+KL ++IN+T+ENV Sbjct: 54 KSKVTVVGSGNWGSVAAKLIASNALKLPSFHDEVRMWVFEEVLPNGEKLNDVINKTNENV 113 Query: 295 KYLPGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAALSLIKG 474 KYLPG KL NVVA PD+ A KDA++L+FV PHQF+ IC L GKI A+SL+KG Sbjct: 114 KYLPGIKLGRNVVADPDLENAVKDANMLVFVTPHQFMDGICKKLDGKITGDVEAISLVKG 173 Query: 475 FDIAEGGGIDLISHIITRCLKIPCAVLMGANIASEVAEEKFCETTIGCRDVM-LAPLMRD 651 ++ + G +IS +I++ L I C VLMGANIA+E+A EKF E T+G R +A Sbjct: 174 MEVKKEGPC-MISSLISKQLGINCCVLMGANIANEIAVEKFSEATVGYRGSREIADTWVQ 232 Query: 652 IIQTDYFRVVVVDD 693 + T YF V V D Sbjct: 233 LFSTPYFMVTPVHD 246 >At2g40690.1 68415.m05020 NAD-dependent glycerol-3-phosphate dehydrogenase family protein low similarity to SP|Q26756 Glycerol-3-phosphate dehydrogenase [NAD+], glycosomal (EC 1.1.1.8) {Trypanosoma brucei rhodesiense}; contains Pfam profile PF01210: NAD-dependent glycerol-3-phosphate dehydrogenase Length = 420 Score = 83.4 bits (197), Expect = 1e-16 Identities = 58/193 (30%), Positives = 88/193 (45%), Gaps = 3/193 (1%) Frame = +1 Query: 124 KVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKLTEIINETHENVKYL 303 KV ++G G++G+A+A V R L V M V + + + INE H N KY Sbjct: 89 KVVVLGGGSFGTAMAAHVARRKEGLE-----VNMLVRDSFV-----CQSINENHHNCKYF 138 Query: 304 PGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAALSLIKGFDI 483 P HKLP NV+A D A DAD + VP QF + + + P +SL KG ++ Sbjct: 139 PEHKLPENVIATTDAKAALLDADYCLHAVPVQFSSSFLEGIADYVDPGLPFISLSKGLEL 198 Query: 484 AEGGGIDLISHIITRCLK---IPCAVLMGANIASEVAEEKFCETTIGCRDVMLAPLMRDI 654 + ++S II LK P L G + A E+ + +D LA ++ + Sbjct: 199 ---NTLRMMSQIIPIALKNPRQPFVALSGPSFALELMNNLPTAMVVASKDKKLANAVQQL 255 Query: 655 IQTDYFRVVVVDD 693 + + Y R+ D Sbjct: 256 LASSYLRINTSSD 268 >At5g63910.1 68418.m08025 expressed protein Length = 500 Score = 32.7 bits (71), Expect = 0.24 Identities = 18/55 (32%), Positives = 30/55 (54%), Gaps = 1/55 (1%) Frame = +1 Query: 127 VCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKLT-EIINETHE 288 VCIVGSG GS++A + + S + ++ M+ EI+ G+ T + +T E Sbjct: 38 VCIVGSGIGGSSVAHFLRNYSVSTGLNQAKILMFERHEIVGGRMRTVTVAGDTFE 92 >At5g66690.1 68418.m08407 UDP-glucoronosyl/UDP-glucosyl transferase family protein contains Pfam profile: PF00201 UDP-glucoronosyl and UDP-glucosyl transferase Length = 481 Score = 30.7 bits (66), Expect = 0.97 Identities = 22/80 (27%), Positives = 37/80 (46%), Gaps = 5/80 (6%) Frame = +1 Query: 82 DCNILDMADKQPKNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKL 261 D +LD ++QP V + G+ G AK + A L + R +WV ++G Sbjct: 250 DHPVLDWLNEQPNESVLYISFGSGGCLSAKQLTELAWGLEQSQQRF-VWVVRPPVDGSCC 308 Query: 262 TEIINE----THENV-KYLP 306 +E ++ T +N +YLP Sbjct: 309 SEYVSANGGGTEDNTPEYLP 328 >At3g50740.1 68416.m05552 UDP-glucoronosyl/UDP-glucosyl transferase family protein contains Pfam profile: PF00201 UDP-glucoronosyl and UDP-glucosyl transferase Length = 487 Score = 30.7 bits (66), Expect = 0.97 Identities = 20/82 (24%), Positives = 35/82 (42%) Frame = +1 Query: 91 ILDMADKQPKNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKLTEI 270 +LD +KQP V + G+ GS AK + A L + R +WV ++G + Sbjct: 258 VLDWLNKQPDESVLYISFGSGGSLSAKQLTELAWGLEMSQQRF-VWVVRPPVDGSACSAY 316 Query: 271 INETHENVKYLPGHKLPSNVVA 336 ++ ++ LP V+ Sbjct: 317 LSANSGKIRDGTPDYLPEGFVS 338 >At5g51490.1 68418.m06386 pectinesterase family protein contains Pfam profile: PF01095 pectinesterase Length = 536 Score = 29.1 bits (62), Expect = 3.0 Identities = 15/47 (31%), Positives = 24/47 (51%) Frame = +1 Query: 508 ISHIITRCLKIPCAVLMGANIASEVAEEKFCETTIGCRDVMLAPLMR 648 ISH+I+ CL + A+L N + A +K T + +D L +R Sbjct: 173 ISHLISNCLAVNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLRAVR 219 >At2g29770.1 68415.m03617 kelch repeat-containing F-box family protein contains Pfam PF00646: F-box domain; contains Pfam PF01344 : Kelch motif; similar to SKP1 interacting partner 6 (GI:10716957) [Arabidopsis thaliana] Length = 387 Score = 29.1 bits (62), Expect = 3.0 Identities = 12/22 (54%), Positives = 15/22 (68%) Frame = +3 Query: 552 INGSQYCIGGC*GKILRNDHWL 617 I+G Y IGGC + RNDHW+ Sbjct: 181 IDGRIYVIGGC---VKRNDHWI 199 >At2g17420.1 68415.m02010 thioredoxin reductase 2 / NADPH-dependent thioredoxin reductase 2 (NTR2) identical to SP|Q39242 Length = 330 Score = 29.1 bits (62), Expect = 3.0 Identities = 26/79 (32%), Positives = 35/79 (44%) Frame = +1 Query: 118 KNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKLTEIINETHENVK 297 K KVCIVGSG A R FE W+ +I G +LT T +V+ Sbjct: 5 KTKVCIVGSGPAAHTAAIYASRAELKPLLFEG----WMANDIAPGGQLT-----TTTDVE 55 Query: 298 YLPGHKLPSNVVAVPDVVE 354 PG P ++ + D+VE Sbjct: 56 NFPG--FPEGILGI-DIVE 71 >At4g23460.1 68417.m03381 beta-adaptin, putative strong similarity to SP|Q10567 Adapter-related protein complex 1 beta 1 subunit (Beta-adaptin 1) [Homo sapiens], beta-adaptin [Drosophila melanogaster] GI:434902; contains Pfam profile: PF01602 Adaptin N terminal region Length = 893 Score = 27.9 bits (59), Expect = 6.8 Identities = 11/25 (44%), Positives = 18/25 (72%) Frame = -2 Query: 638 GASITSRQPMVVSQNFSSATSDAIL 564 GAS + PMV+SQN S+ ++ ++L Sbjct: 733 GASARTMMPMVLSQNMSTGSTSSVL 757 >At5g07800.1 68418.m00894 flavin-containing monooxygenase family protein / FMO family protein similar to flavin-containing monooxygenase 2 (FMO2) from Homo sapiens [GI:1834493]; contains Pfam profile: PF00743 Flavin-binding monooxygenase-like Length = 460 Score = 27.5 bits (58), Expect = 9.0 Identities = 15/52 (28%), Positives = 23/52 (44%), Gaps = 3/52 (5%) Frame = +1 Query: 103 ADKQPKNKVCIVGSGNWGSAIAKIV---GRNAASLSNFEDRVTMWVYEEIIE 249 A + KVC++G+G G A+ + G L ED W Y+ +E Sbjct: 8 ASRSRSKKVCVIGAGPAGLVSARELRKEGHKVVVLEQNEDVGGQWFYQPNVE 59 >At4g39290.1 68417.m05564 kelch repeat-containing F-box family protein contains F-box domain Pfam:PF00646 and Kelch motif Pfam:PF01344 Length = 365 Score = 27.5 bits (58), Expect = 9.0 Identities = 12/26 (46%), Positives = 16/26 (61%) Frame = +3 Query: 540 SLCCINGSQYCIGGC*GKILRNDHWL 617 S C +NG Y IGGC K L + +W+ Sbjct: 160 STCVLNGKIYVIGGC--KNLDSTNWI 183 >At2g26890.1 68415.m03226 DNAJ heat shock N-terminal domain-containing protein contains Pfam profile PF00226: DnaJ domain Length = 2554 Score = 27.5 bits (58), Expect = 9.0 Identities = 12/30 (40%), Positives = 20/30 (66%) Frame = +1 Query: 445 TAAALSLIKGFDIAEGGGIDLISHIITRCL 534 T AA SL G ++ GG+ L+S +++RC+ Sbjct: 1652 TCAASSL-NGEELVRDGGVQLLSTLLSRCM 1680 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,565,056 Number of Sequences: 28952 Number of extensions: 266472 Number of successful extensions: 871 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 845 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 869 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1496852856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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