Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30637
(700 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g40610.1 68418.m04930 glycerol-3-phosphate dehydrogenase [NAD... 193 1e-49
At2g40690.1 68415.m05020 NAD-dependent glycerol-3-phosphate dehy... 83 2e-16
At5g63910.1 68418.m08025 expressed protein 33 0.24
At5g66690.1 68418.m08407 UDP-glucoronosyl/UDP-glucosyl transfera... 31 0.97
At3g50740.1 68416.m05552 UDP-glucoronosyl/UDP-glucosyl transfera... 31 0.97
At5g51490.1 68418.m06386 pectinesterase family protein contains ... 29 3.0
At2g29770.1 68415.m03617 kelch repeat-containing F-box family pr... 29 3.0
At2g17420.1 68415.m02010 thioredoxin reductase 2 / NADPH-depende... 29 3.0
At4g23460.1 68417.m03381 beta-adaptin, putative strong similarit... 28 6.8
At5g07800.1 68418.m00894 flavin-containing monooxygenase family ... 27 9.0
At4g39290.1 68417.m05564 kelch repeat-containing F-box family pr... 27 9.0
At2g26890.1 68415.m03226 DNAJ heat shock N-terminal domain-conta... 27 9.0
>At5g40610.1 68418.m04930 glycerol-3-phosphate dehydrogenase [NAD+]
/ GPDH strong similarity to SP|P52425
Glycerol-3-phosphate dehydrogenase [NAD+] (EC 1.1.1.8)
{Cuphea lanceolata}; contains Pfam profile PF01210:
NAD-dependent glycerol-3-phosphate dehydrogenase
Length = 400
Score = 193 bits (470), Expect = 1e-49
Identities = 97/189 (51%), Positives = 129/189 (68%), Gaps = 2/189 (1%)
Frame = +1
Query: 118 KNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEII-EGKKLTEIINETHENV 294
K+KV +VGSGNWGS AK++ NA L +F D V MWV+EE++ G+KL ++IN+T+ENV
Sbjct: 54 KSKVTVVGSGNWGSVAAKLIASNALKLPSFHDEVRMWVFEEVLPNGEKLNDVINKTNENV 113
Query: 295 KYLPGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAALSLIKG 474
KYLPG KL NVVA PD+ A KDA++L+FV PHQF+ IC L GKI A+SL+KG
Sbjct: 114 KYLPGIKLGRNVVADPDLENAVKDANMLVFVTPHQFMDGICKKLDGKITGDVEAISLVKG 173
Query: 475 FDIAEGGGIDLISHIITRCLKIPCAVLMGANIASEVAEEKFCETTIGCRDVM-LAPLMRD 651
++ + G +IS +I++ L I C VLMGANIA+E+A EKF E T+G R +A
Sbjct: 174 MEVKKEGPC-MISSLISKQLGINCCVLMGANIANEIAVEKFSEATVGYRGSREIADTWVQ 232
Query: 652 IIQTDYFRV 678
+ T YF V
Sbjct: 233 LFSTPYFMV 241
>At2g40690.1 68415.m05020 NAD-dependent glycerol-3-phosphate
dehydrogenase family protein low similarity to SP|Q26756
Glycerol-3-phosphate dehydrogenase [NAD+], glycosomal
(EC 1.1.1.8) {Trypanosoma brucei rhodesiense}; contains
Pfam profile PF01210: NAD-dependent glycerol-3-phosphate
dehydrogenase
Length = 420
Score = 83.0 bits (196), Expect = 2e-16
Identities = 57/188 (30%), Positives = 87/188 (46%), Gaps = 3/188 (1%)
Frame = +1
Query: 124 KVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKLTEIINETHENVKYL 303
KV ++G G++G+A+A V R L V M V + + + INE H N KY
Sbjct: 89 KVVVLGGGSFGTAMAAHVARRKEGLE-----VNMLVRDSFV-----CQSINENHHNCKYF 138
Query: 304 PGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAALSLIKGFDI 483
P HKLP NV+A D A DAD + VP QF + + + P +SL KG ++
Sbjct: 139 PEHKLPENVIATTDAKAALLDADYCLHAVPVQFSSSFLEGIADYVDPGLPFISLSKGLEL 198
Query: 484 AEGGGIDLISHIITRCLK---IPCAVLMGANIASEVAEEKFCETTIGCRDVMLAPLMRDI 654
+ ++S II LK P L G + A E+ + +D LA ++ +
Sbjct: 199 ---NTLRMMSQIIPIALKNPRQPFVALSGPSFALELMNNLPTAMVVASKDKKLANAVQQL 255
Query: 655 IQTDYFRV 678
+ + Y R+
Sbjct: 256 LASSYLRI 263
>At5g63910.1 68418.m08025 expressed protein
Length = 500
Score = 32.7 bits (71), Expect = 0.24
Identities = 18/55 (32%), Positives = 30/55 (54%), Gaps = 1/55 (1%)
Frame = +1
Query: 127 VCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKLT-EIINETHE 288
VCIVGSG GS++A + + S + ++ M+ EI+ G+ T + +T E
Sbjct: 38 VCIVGSGIGGSSVAHFLRNYSVSTGLNQAKILMFERHEIVGGRMRTVTVAGDTFE 92
>At5g66690.1 68418.m08407 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 481
Score = 30.7 bits (66), Expect = 0.97
Identities = 22/80 (27%), Positives = 37/80 (46%), Gaps = 5/80 (6%)
Frame = +1
Query: 82 DCNILDMADKQPKNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKL 261
D +LD ++QP V + G+ G AK + A L + R +WV ++G
Sbjct: 250 DHPVLDWLNEQPNESVLYISFGSGGCLSAKQLTELAWGLEQSQQRF-VWVVRPPVDGSCC 308
Query: 262 TEIINE----THENV-KYLP 306
+E ++ T +N +YLP
Sbjct: 309 SEYVSANGGGTEDNTPEYLP 328
>At3g50740.1 68416.m05552 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 487
Score = 30.7 bits (66), Expect = 0.97
Identities = 20/82 (24%), Positives = 35/82 (42%)
Frame = +1
Query: 91 ILDMADKQPKNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKLTEI 270
+LD +KQP V + G+ GS AK + A L + R +WV ++G +
Sbjct: 258 VLDWLNKQPDESVLYISFGSGGSLSAKQLTELAWGLEMSQQRF-VWVVRPPVDGSACSAY 316
Query: 271 INETHENVKYLPGHKLPSNVVA 336
++ ++ LP V+
Sbjct: 317 LSANSGKIRDGTPDYLPEGFVS 338
>At5g51490.1 68418.m06386 pectinesterase family protein contains
Pfam profile: PF01095 pectinesterase
Length = 536
Score = 29.1 bits (62), Expect = 3.0
Identities = 15/47 (31%), Positives = 24/47 (51%)
Frame = +1
Query: 508 ISHIITRCLKIPCAVLMGANIASEVAEEKFCETTIGCRDVMLAPLMR 648
ISH+I+ CL + A+L N + A +K T + +D L +R
Sbjct: 173 ISHLISNCLAVNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLRAVR 219
>At2g29770.1 68415.m03617 kelch repeat-containing F-box family
protein contains Pfam PF00646: F-box domain; contains
Pfam PF01344 : Kelch motif; similar to SKP1 interacting
partner 6 (GI:10716957) [Arabidopsis thaliana]
Length = 387
Score = 29.1 bits (62), Expect = 3.0
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = +3
Query: 552 INGSQYCIGGC*GKILRNDHWL 617
I+G Y IGGC + RNDHW+
Sbjct: 181 IDGRIYVIGGC---VKRNDHWI 199
>At2g17420.1 68415.m02010 thioredoxin reductase 2 / NADPH-dependent
thioredoxin reductase 2 (NTR2) identical to SP|Q39242
Length = 330
Score = 29.1 bits (62), Expect = 3.0
Identities = 26/79 (32%), Positives = 35/79 (44%)
Frame = +1
Query: 118 KNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKLTEIINETHENVK 297
K KVCIVGSG A R FE W+ +I G +LT T +V+
Sbjct: 5 KTKVCIVGSGPAAHTAAIYASRAELKPLLFEG----WMANDIAPGGQLT-----TTTDVE 55
Query: 298 YLPGHKLPSNVVAVPDVVE 354
PG P ++ + D+VE
Sbjct: 56 NFPG--FPEGILGI-DIVE 71
>At4g23460.1 68417.m03381 beta-adaptin, putative strong similarity
to SP|Q10567 Adapter-related protein complex 1 beta 1
subunit (Beta-adaptin 1) [Homo sapiens], beta-adaptin
[Drosophila melanogaster] GI:434902; contains Pfam
profile: PF01602 Adaptin N terminal region
Length = 893
Score = 27.9 bits (59), Expect = 6.8
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = -3
Query: 638 GASITSRQPMVVSQNFSSATSDAIL 564
GAS + PMV+SQN S+ ++ ++L
Sbjct: 733 GASARTMMPMVLSQNMSTGSTSSVL 757
>At5g07800.1 68418.m00894 flavin-containing monooxygenase family
protein / FMO family protein similar to
flavin-containing monooxygenase 2 (FMO2) from Homo
sapiens [GI:1834493]; contains Pfam profile: PF00743
Flavin-binding monooxygenase-like
Length = 460
Score = 27.5 bits (58), Expect = 9.0
Identities = 15/52 (28%), Positives = 23/52 (44%), Gaps = 3/52 (5%)
Frame = +1
Query: 103 ADKQPKNKVCIVGSGNWGSAIAKIV---GRNAASLSNFEDRVTMWVYEEIIE 249
A + KVC++G+G G A+ + G L ED W Y+ +E
Sbjct: 8 ASRSRSKKVCVIGAGPAGLVSARELRKEGHKVVVLEQNEDVGGQWFYQPNVE 59
>At4g39290.1 68417.m05564 kelch repeat-containing F-box family
protein contains F-box domain Pfam:PF00646 and Kelch
motif Pfam:PF01344
Length = 365
Score = 27.5 bits (58), Expect = 9.0
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = +3
Query: 540 SLCCINGSQYCIGGC*GKILRNDHWL 617
S C +NG Y IGGC K L + +W+
Sbjct: 160 STCVLNGKIYVIGGC--KNLDSTNWI 183
>At2g26890.1 68415.m03226 DNAJ heat shock N-terminal domain-containing
protein contains Pfam profile PF00226: DnaJ domain
Length = 2554
Score = 27.5 bits (58), Expect = 9.0
Identities = 12/30 (40%), Positives = 20/30 (66%)
Frame = +1
Query: 445 TAAALSLIKGFDIAEGGGIDLISHIITRCL 534
T AA SL G ++ GG+ L+S +++RC+
Sbjct: 1652 TCAASSL-NGEELVRDGGVQLLSTLLSRCM 1680
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,193,315
Number of Sequences: 28952
Number of extensions: 281926
Number of successful extensions: 911
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 885
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 909
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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