Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30627
         (774 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo...    56   7e-09
SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual      49   6e-07
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom...    45   1e-05
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar...    32   0.10 
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po...    28   1.7  
SPAC23G3.01 |rpb2|SPAC521.06|DNA-directed RNA polymerase II comp...    27   3.0  
SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr...    25   9.1  
SPCC126.07c |||human CTD-binding SR-like protein rA9 homolog|Sch...    25   9.1  
SPAC4G9.04c |||cleavage and polyadenylation specificity factor |...    25   9.1  
SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosac...    25   9.1  

>SPAC29A4.11 |rga3||GTPase activating protein
           Rga3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 969

 Score = 55.6 bits (128), Expect = 7e-09
 Identities = 33/104 (31%), Positives = 44/104 (42%), Gaps = 3/104 (2%)
 Frame = +3

Query: 252 WHSGHFCCWQCDESLTGQRYVL---RDEHPYCIKCYESVFANGCEECNKIIGIDSKDLSY 422
           WH   FCC +CD+ L     +L    D  P C  C     A+ C  C   I  D   +S 
Sbjct: 40  WHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSSC-----AHTCTACRMRIK-DYALMSG 93

Query: 423 KDKHWHEACFLCAKCRVSLVDKQFGSKLDKIYCGNCYDAQFASR 554
            D + H  CF C  CR  ++D  F      I+C +C   +  SR
Sbjct: 94  YDSY-HRECFRCHDCRKQIIDSNFKRDNRTIFCNDCKQVRHPSR 136



 Score = 31.9 bits (69), Expect = 0.10
 Identities = 24/99 (24%), Positives = 37/99 (37%), Gaps = 4/99 (4%)
 Frame = +3

Query: 372 CEECNKIIGIDSKDLSYKDKHWHEACFLCAKCRVSL--VDKQFGSKLD-KIYCGNCYDAQ 542
           C  C +        +S+    WH+ CF C KC   L   D+      D +  C +C    
Sbjct: 19  CFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSSC---- 74

Query: 543 FASRCDGCGEVFRAGTKKMEYKTRQWHEKCF-CLRCLQE 656
            A  C  C    +       Y +  +H +CF C  C ++
Sbjct: 75  -AHTCTACRMRIKDYALMSGYDS--YHRECFRCHDCRKQ 110


>SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 438

 Score = 49.2 bits (112), Expect = 6e-07
 Identities = 36/144 (25%), Positives = 59/144 (40%), Gaps = 2/144 (1%)
 Frame = +3

Query: 222 GEYTKAMNKDWHSGHFCCWQCDESLTGQRYVLRDEHPYCIKCYESVFANGCEECNKIIGI 401
           G    A  K  H   F C  C ++L    +  R+   YC   Y   F+  C+ C     I
Sbjct: 268 GRIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGKFYCHLDYHEQFSPRCKHCK--TPI 325

Query: 402 DSKDLSYKDKHWHEACFLCAKC-RVSLVDKQFGSKLDKIYCGNCYDAQFASRCDGCGEVF 578
           + + +   +  +HE    CA C  V  V+     + D  +C  CYD ++A +C  C +  
Sbjct: 326 EDQAVHINNDWFHENHHFCAGCSEVFNVNIPCIYRDDLYWCQTCYDNKYAVKCKKCRKPI 385

Query: 579 RAGTKKMEYKTRQWHEKCF-CLRC 647
              + K      ++H +C+ C  C
Sbjct: 386 LGISVK--GSDGEYHSQCWTCGAC 407



 Score = 37.9 bits (84), Expect = 0.002
 Identities = 21/73 (28%), Positives = 27/73 (36%)
 Frame = +3

Query: 348 YESVFANGCEECNKIIGIDSKDLSYKDKHWHEACFLCAKCRVSLVDKQFGSKLDKIYCGN 527
           Y       C  C   +    + +S   K  H  CF C  C  +L    F  +  K YC  
Sbjct: 250 YRGNSEKSCHSCGGSLRA-GRIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGKFYCHL 308

Query: 528 CYDAQFASRCDGC 566
            Y  QF+ RC  C
Sbjct: 309 DYHEQFSPRCKHC 321



 Score = 32.7 bits (71), Expect = 0.060
 Identities = 11/26 (42%), Positives = 17/26 (65%)
 Frame = +1

Query: 679 IPREQEIYCAGCYEDKFATRCVKCNK 756
           I R+   +C  CY++K+A +C KC K
Sbjct: 358 IYRDDLYWCQTCYDNKYAVKCKKCRK 383



 Score = 29.5 bits (63), Expect = 0.56
 Identities = 12/37 (32%), Positives = 17/37 (45%)
 Frame = +1

Query: 640 CVVCKNPIGTKSFIPREQEIYCAGCYEDKFATRCVKC 750
           C  C   +    F  RE + YC   Y ++F+ RC  C
Sbjct: 285 CDTCSQNLEHVGFYYREGKFYCHLDYHEQFSPRCKHC 321



 Score = 25.8 bits (54), Expect = 6.9
 Identities = 11/42 (26%), Positives = 19/42 (45%)
 Frame = +3

Query: 222 GEYTKAMNKDWHSGHFCCWQCDESLTGQRYVLRDEHPYCIKC 347
           G   K  + ++HS  + C  C+  L  + Y + +  P C  C
Sbjct: 387 GISVKGSDGEYHSQCWTCGACNALLGDEGYFMIENTPICRPC 428


>SPBC3F6.05 |rga1||GTPase activating protein
           Rga1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1150

 Score = 44.8 bits (101), Expect = 1e-05
 Identities = 35/145 (24%), Positives = 58/145 (40%), Gaps = 8/145 (5%)
 Frame = +3

Query: 222 GEYTKAMNKDWHSGHFCCWQCDESLTGQRYVLRD-----EHPYCIKCYESVFANGCEECN 386
           G+Y +A+   +H   F C  C+  +  + + + D     + P C   Y       C  C 
Sbjct: 125 GQYVRALGNIYHLECFRCHDCNSLVASKFFPIDDPTLNKQVPLCETDYFRRLDLLCASCG 184

Query: 387 KIIGIDSKDLSYKDKHWHEACFLCAKC-RVSLVDKQFGSKLDKIYCGNCYDAQFASRCDG 563
             + +    ++  +K +H   F C+ C  V   +  +     K+YC   Y   FA+RC G
Sbjct: 185 --MALRGYYITALNKKFHIEHFTCSLCYTVFGPNDSYYEYEGKVYCHYHYSTLFAARCCG 242

Query: 564 C-GEVFRAGTKKMEYKTRQ-WHEKC 632
           C G + R   +       Q WH  C
Sbjct: 243 CDGPILRQFVEVYRNGVSQNWHVPC 267



 Score = 28.3 bits (60), Expect = 1.3
 Identities = 13/39 (33%), Positives = 18/39 (46%), Gaps = 1/39 (2%)
 Frame = +1

Query: 640 CVVCKNPIGTK-SFIPREQEIYCAGCYEDKFATRCVKCN 753
           C +C    G   S+   E ++YC   Y   FA RC  C+
Sbjct: 206 CSLCYTVFGPNDSYYEYEGKVYCHYHYSTLFAARCCGCD 244


>SPBC28E12.03 |rga4||GTPase activating protein
           Rga4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 933

 Score = 31.9 bits (69), Expect = 0.10
 Identities = 24/81 (29%), Positives = 33/81 (40%), Gaps = 3/81 (3%)
 Frame = +3

Query: 333 YCIKCYESVFANGCEECNKIIGIDSKDLSYKDKHWHEACFLCAKCRVSL--VDKQFGSKL 506
           +CIKC+ESV +             +  + +  K WH  CF C  C   L    + F    
Sbjct: 23  FCIKCWESVPS-------------TSQVWFGGKCWHSDCFKCVNCNKKLDPSSEDFSQDD 69

Query: 507 DK-IYCGNCYDAQFASRCDGC 566
            K I+C  C D      C+GC
Sbjct: 70  QKQIFCKLCVDI-----CNGC 85



 Score = 27.9 bits (59), Expect = 1.7
 Identities = 25/102 (24%), Positives = 40/102 (39%), Gaps = 3/102 (2%)
 Frame = +3

Query: 246 KDWHSGHFCCWQCDESL--TGQRYVLRDEHP-YCIKCYESVFANGCEECNKIIGIDSKDL 416
           K WHS  F C  C++ L  + + +   D+   +C  C +    NGC     I   +    
Sbjct: 42  KCWHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCKLCVD--ICNGCS--TPICEFN---- 93

Query: 417 SYKDKHWHEACFLCAKCRVSLVDKQFGSKLDKIYCGNCYDAQ 542
           +  +   +  C  C+ CR  +    FG    + YC  C   Q
Sbjct: 94  AVSNSQANHPC--CSNCRAFINSNAFGKFKGQHYCLPCLRKQ 133


>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 897

 Score = 27.9 bits (59), Expect = 1.7
 Identities = 11/29 (37%), Positives = 13/29 (44%)
 Frame = +3

Query: 261 GHFCCWQCDESLTGQRYVLRDEHPYCIKC 347
           GHF C +C   L GQ+Y          KC
Sbjct: 623 GHFTCQECMSMLVGQKYGSSSTSTIIAKC 651


>SPAC23G3.01 |rpb2|SPAC521.06|DNA-directed RNA polymerase II complex
           subunit Rpb2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1210

 Score = 27.1 bits (57), Expect = 3.0
 Identities = 17/45 (37%), Positives = 23/45 (51%)
 Frame = -3

Query: 484 STRETRHLAHKKQASCQCLSLYDRSLESMPMILLHSSQPLAKTLS 350
           S R T   A  KQA    L+ Y   +++M  IL +  +PLA T S
Sbjct: 753 SPRNTYQSAMGKQAMGVYLTNYQVRMDTMANILYYPQKPLATTRS 797


>SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 131

 Score = 25.4 bits (53), Expect = 9.1
 Identities = 9/23 (39%), Positives = 12/23 (52%)
 Frame = +3

Query: 420 YKDKHWHEACFLCAKCRVSLVDK 488
           Y   H    C++CAKC+  L  K
Sbjct: 4   YYPVHLKSRCYVCAKCKTHLAFK 26


>SPCC126.07c |||human CTD-binding SR-like protein rA9
           homolog|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 571

 Score = 25.4 bits (53), Expect = 9.1
 Identities = 24/98 (24%), Positives = 44/98 (44%), Gaps = 6/98 (6%)
 Frame = +3

Query: 327 HPYCIKCYESVFANGCEECN-KIIGIDSKDLSYKDKHWHEACFLCAKCR-VSLVDKQF-- 494
           H +C++ +  V AN C  C  + + +D   L +    W+ A  +  K + V+   + F  
Sbjct: 61  HNHCLESWCRV-ANTCPLCRTEFLKVDV--LEFVKGPWYRAYPVEEKTQSVANAGEPFED 117

Query: 495 -GSKLDK-IYCGNCYDAQFASRCDGCGEVFRAGTKKME 602
            GS+  + + CG    A+    CDGC + +      M+
Sbjct: 118 EGSETCRCVICGRSDHAEVLLLCDGCDDAYHTYCLNMD 155


>SPAC4G9.04c |||cleavage and polyadenylation specificity factor
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 638

 Score = 25.4 bits (53), Expect = 9.1
 Identities = 12/33 (36%), Positives = 17/33 (51%), Gaps = 2/33 (6%)
 Frame = +3

Query: 348 YESVFANGCEECNKIIGIDSKDLSYKDKH--WH 440
           YE+ ++N C  C +  G D +     DKH  WH
Sbjct: 416 YEN-YSNQCANCGRRYGNDPESRKELDKHSDWH 447


>SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase
           E3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 905

 Score = 25.4 bits (53), Expect = 9.1
 Identities = 14/41 (34%), Positives = 20/41 (48%)
 Frame = -2

Query: 512 LVELGAELLVDQRDAALGAQEAGLVPVLVLVRQILRVNADD 390
           L  LG ELL +  D     Q A L+   V+ ++ L   A+D
Sbjct: 689 LTPLGMELLQNYIDHTSDVQTAALIAAFVVPKKFLDKRAED 729


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,463,833
Number of Sequences: 5004
Number of extensions: 42663
Number of successful extensions: 141
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 118
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 134
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 373338084
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)