Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30616
(681 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g51010.1 68416.m05585 expressed protein 33 0.23
At3g23930.1 68416.m03006 expressed protein 32 0.40
At5g45520.1 68418.m05591 hypothetical protein 30 1.6
At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains... 30 1.6
At1g48280.1 68414.m05393 hydroxyproline-rich glycoprotein family... 30 1.6
At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / hea... 29 2.2
At5g05210.1 68418.m00555 nucleolar matrix protein-related contai... 29 2.8
At5g03660.1 68418.m00325 expressed protein low similarity to out... 29 2.8
At2g25350.1 68415.m03032 phox (PX) domain-containing protein wea... 29 2.8
At5g41540.1 68418.m05048 disease resistance protein (TIR-NBS-LRR... 29 3.8
At5g25070.1 68418.m02971 expressed protein 29 3.8
At1g01670.1 68414.m00085 U-box domain-containing protein 29 3.8
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 28 6.6
At3g43160.1 68416.m04554 expressed protein merozoite surface pro... 28 6.6
At1g05320.1 68414.m00539 myosin-related similar to non-muscle my... 28 6.6
At2g18540.1 68415.m02160 cupin family protein contains Pfam prof... 27 8.7
At1g63300.1 68414.m07156 expressed protein similar to Intracellu... 27 8.7
>At3g51010.1 68416.m05585 expressed protein
Length = 188
Score = 32.7 bits (71), Expect = 0.23
Identities = 22/66 (33%), Positives = 34/66 (51%)
Frame = +1
Query: 442 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEE 621
I ++R + +KKRQA++Q K + +++K A ER +QLEEE
Sbjct: 125 IGQRRAFILSEKKKRQALVQEAKRKKRIKQ---VERKMAAVARDRAWAERLIELQQLEEE 181
Query: 622 KKISLS 639
KK S+S
Sbjct: 182 KKKSMS 187
>At3g23930.1 68416.m03006 expressed protein
Length = 224
Score = 31.9 bits (69), Expect = 0.40
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = +1
Query: 184 KQEGEGDPEFIKRQDQKRSDLDEQLKEYINEWRKQR 291
K + G IK +D++ + QLKE EWRK+R
Sbjct: 21 KDQSRGRRHLIKERDEREKVMFLQLKEAEREWRKER 56
>At5g45520.1 68418.m05591 hypothetical protein
Length = 1167
Score = 29.9 bits (64), Expect = 1.6
Identities = 19/68 (27%), Positives = 36/68 (52%), Gaps = 5/68 (7%)
Frame = +1
Query: 439 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENF-----GLSNAQLERNKTK 603
++E+ ++ E E++ + +++ KT P+ +K +N G SN + + +K K
Sbjct: 624 NLEDGKKHDEGKEERSLKSDEVVEEEKKTSPSEEATEKFQNKPGDQKGKSNVEGDGDKGK 683
Query: 604 EQLEEEKK 627
LEEEKK
Sbjct: 684 ADLEEEKK 691
>At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains
ATP/GTP-binding site motif A (P-loop), PROSITE:PS00017
Length = 1340
Score = 29.9 bits (64), Expect = 1.6
Identities = 17/69 (24%), Positives = 36/69 (52%), Gaps = 1/69 (1%)
Frame = +1
Query: 442 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNA-QLERNKTKEQLEE 618
++ K++ KK++ + K +KTG + +KK+ + +S +++RN T E+ +
Sbjct: 898 VDVKQETGSPDTKKKEGASSSSKKDTKTGEDKKAEKKNNSETMSEGKKIDRNNTDEKEVK 957
Query: 619 EKKISLSIR 645
EK I+
Sbjct: 958 EKVTEKEIK 966
>At1g48280.1 68414.m05393 hydroxyproline-rich glycoprotein family
protein
Length = 558
Score = 29.9 bits (64), Expect = 1.6
Identities = 26/74 (35%), Positives = 37/74 (50%), Gaps = 4/74 (5%)
Frame = +1
Query: 445 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTK---EQLE 615
+EKR+R+EE E+K +KD N ++ + E SN +LE N K + +
Sbjct: 112 DEKRKRMEELEEKLVVNESLIKDLQLQVLN--LKTELEEARNSNVELELNNRKLSQDLVS 169
Query: 616 EEKKI-SLSIRIKP 654
E KI SLS KP
Sbjct: 170 AEAKISSLSSNDKP 183
>At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / heat
shock protein 83 (HSP83) nearly identical to SP|P27323
Heat shock protein 81-1 (HSP81-1) (Heat shock protein
83) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 705
Score = 29.5 bits (63), Expect = 2.2
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = +1
Query: 181 PKQEGEGDPEFIKRQDQKRSDLDEQLKEYINEW 279
PK+E EG+ E + + +K +++KE +EW
Sbjct: 232 PKKENEGEVEEVDEEKEKDGKKKKKIKEVSHEW 264
>At5g05210.1 68418.m00555 nucleolar matrix protein-related contains
Pfam domain, PF04935: Surfeit locus protein 6
Length = 386
Score = 29.1 bits (62), Expect = 2.8
Identities = 12/59 (20%), Positives = 33/59 (55%)
Frame = +1
Query: 454 RQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEKKI 630
+Q + + +K+++ + K+ + F ++K+ + G ++E+NK ++ + EKK+
Sbjct: 310 KQSIHKDKKRQEKNAEKWKERIEGQQKFKVEKQQKRSGNIADRIEQNKMRKIAKREKKL 368
>At5g03660.1 68418.m00325 expressed protein low similarity to outer
surface protein F [Borrelia burgdorferi] GI:466482;
contains Pfam profile PF04949: Family of unknown
function (DUF662)
Length = 173
Score = 29.1 bits (62), Expect = 2.8
Identities = 16/49 (32%), Positives = 26/49 (53%)
Frame = +1
Query: 505 MKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEKKISLSIRIK 651
+KD +F+ Q K E+ LS L K KE+ E+KK+ + R++
Sbjct: 18 LKDQLSESMSFSSQMKKEDDELSMKALSAFKAKEEEIEKKKMEIRERVQ 66
>At2g25350.1 68415.m03032 phox (PX) domain-containing protein weak
similarity to SP|Q9UTK5 Abnormal long morphology protein
1 (Sp8) {Schizosaccharomyces pombe}; contains Pfam
profile PF00787: PX domain
Length = 643
Score = 29.1 bits (62), Expect = 2.8
Identities = 23/71 (32%), Positives = 34/71 (47%)
Frame = +1
Query: 439 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEE 618
D+EE RQ+ E E K ++ D SKT N TI + E E + TK+QLE+
Sbjct: 455 DMEELRQKTFEMELKLKSKEDGSSD-SKTSGNSTISESHE------LLQEMDATKQQLED 507
Query: 619 EKKISLSIRIK 651
+ + + K
Sbjct: 508 LSRRYVELEAK 518
>At5g41540.1 68418.m05048 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1038
Score = 28.7 bits (61), Expect = 3.8
Identities = 19/50 (38%), Positives = 24/50 (48%)
Frame = +1
Query: 439 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLE 588
D EKRQ L EAE+ R + S G +F + K SE F +S E
Sbjct: 502 DEPEKRQFLVEAEEIRDVLANETGTGSVLGISFDMSKVSE-FSISGRAFE 550
>At5g25070.1 68418.m02971 expressed protein
Length = 736
Score = 28.7 bits (61), Expect = 3.8
Identities = 22/67 (32%), Positives = 32/67 (47%), Gaps = 1/67 (1%)
Frame = +1
Query: 448 EKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEE-EK 624
EKR EAEKK A + K+A + + KS N Q+E K +LE+ E
Sbjct: 565 EKRMPELEAEKKVAASTRNFKEAGR----IAAEAKSLNLEKDKTQMETGKANAELEKAEH 620
Query: 625 KISLSIR 645
+I +I+
Sbjct: 621 EIEETIK 627
>At1g01670.1 68414.m00085 U-box domain-containing protein
Length = 365
Score = 28.7 bits (61), Expect = 3.8
Identities = 19/73 (26%), Positives = 36/73 (49%), Gaps = 6/73 (8%)
Frame = +1
Query: 445 EEKRQRLEEAEKKRQ-----AMLQAMKDASKTGPNFT-IQKKSENFGLSNAQLERNKTKE 606
EE+R+RLE E KR+ M + ++A + T I E + E N+ K
Sbjct: 177 EEQRRRLEIEELKREKEQRDKMRRVREEALSSSSGVTKILYNEEVMRRREVEAELNRAKA 236
Query: 607 QLEEEKKISLSIR 645
++E+ K++ + ++
Sbjct: 237 EIEDMKRVQIELK 249
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 27.9 bits (59), Expect = 6.6
Identities = 20/71 (28%), Positives = 33/71 (46%), Gaps = 2/71 (2%)
Frame = +1
Query: 466 EEAEKKRQAMLQAMKDASKTGPNFT--IQKKSENFGLSNAQLERNKTKEQLEEEKKISLS 639
EEA+K R+ L+ KDAS + + KK+E + +KE E+ ++ +
Sbjct: 710 EEAKKTREQALRKRKDASFKHVIISEKVDKKAEKLQTKSLPYPYT-SKEVFEQSMRMPIG 768
Query: 640 IRIKPLTIEGL 672
P TI G+
Sbjct: 769 PEFNPTTIVGV 779
>At3g43160.1 68416.m04554 expressed protein merozoite surface
protein 2 (MSP-2), EMBL:PFU72951, Plasmodium falciparum
Length = 295
Score = 27.9 bits (59), Expect = 6.6
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = +2
Query: 212 SSSVKTRSGRTWMNS*RNTSTNGANSGPRRRMSSNALKRSR 334
+S+ +TRSGR +++ +ST NS R SS K R
Sbjct: 103 NSTTRTRSGRGFIDYVLESSTGTENSSTRTAESSTRTKNGR 143
>At1g05320.1 68414.m00539 myosin-related similar to non-muscle
myosin II heavy chain (GI:19879404) [Loligo pealei];
ESTs gb|AA042402,gb|ATTS1380 come from this gene
Length = 828
Score = 27.9 bits (59), Expect = 6.6
Identities = 18/68 (26%), Positives = 35/68 (51%), Gaps = 3/68 (4%)
Frame = +1
Query: 442 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKT---KEQL 612
+E+K + LEE +K + A+ A + G +IQ + +S + N++ +L
Sbjct: 155 LEQKLKSLEELSEKVSELKSALIVAEEEGKKSSIQMQEYQEKVSKLESSLNQSSARNSEL 214
Query: 613 EEEKKISL 636
EE+ +I+L
Sbjct: 215 EEDLRIAL 222
>At2g18540.1 68415.m02160 cupin family protein contains Pfam profile
PF00190: Cupin
Length = 707
Score = 27.5 bits (58), Expect = 8.7
Identities = 19/64 (29%), Positives = 34/64 (53%), Gaps = 4/64 (6%)
Frame = +1
Query: 448 EKRQRLEE-AEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQL---E 615
E+R++ EE A K+ +A + ++A + T +KK E + ER + +E+ E
Sbjct: 444 ERRRKEEEEARKREEAKRREEEEAKRREEEETERKKREEEEARKREEERKREEEEAKRRE 503
Query: 616 EEKK 627
EE+K
Sbjct: 504 EERK 507
Score = 27.5 bits (58), Expect = 8.7
Identities = 11/61 (18%), Positives = 32/61 (52%)
Frame = +1
Query: 445 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEK 624
EE+++ EEA+++ + + ++A + ++K E + + K +E++E ++
Sbjct: 490 EERKREEEEAKRREEERKKREEEAEQARKREEEREKEEEMAKKREEERQRKEREEVERKR 549
Query: 625 K 627
+
Sbjct: 550 R 550
>At1g63300.1 68414.m07156 expressed protein similar to Intracellular
protein transport protein USO1 (Swiss-Prot:P25386)
[Saccharomyces cerevisiae]; similar to Myosin II heavy
chain, non muscle (Swiss-Prot:P08799) [Dictyostelium
discoideum]
Length = 1029
Score = 27.5 bits (58), Expect = 8.7
Identities = 21/80 (26%), Positives = 36/80 (45%)
Frame = +1
Query: 442 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEE 621
+ E ++L + + ML+ + + S N QK+ E +N E KE++E
Sbjct: 684 LHELSEKLSFKTSQMERMLENLDEKSNEIDN---QKRHEEDVTANLNQEIKILKEEIENL 740
Query: 622 KKISLSIRIKPLTIEGLSVD 681
KK S+ ++ E L VD
Sbjct: 741 KKNQDSLMLQAEQAENLRVD 760
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.315 0.130 0.361
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,299,902
Number of Sequences: 28952
Number of extensions: 157706
Number of successful extensions: 653
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 629
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 653
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1438152744
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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