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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30604
         (745 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...   105   5e-24
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    60   3e-10
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce...    59   6e-10
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    56   7e-09
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    41   2e-04
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi...    33   0.043
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos...    31   0.17 
SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces p...    29   0.53 
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha...    27   2.8  
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch...    26   6.5  
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual    26   6.5  
SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma...    25   8.6  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score =  105 bits (253), Expect = 5e-24
 Identities = 52/148 (35%), Positives = 88/148 (59%)
 Frame = +3

Query: 54  LPPEQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRL 233
           L  EQIA  R+AF  FD ++ G+I  + +  ++R +GQ      L+++I EVDAD +G +
Sbjct: 6   LTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTI 65

Query: 234 EFPEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXXX 413
           +F EF+T+ A+ + + D E   +E+REAF+++DK+GNGYI    L  +            
Sbjct: 66  DFTEFLTMMARKMKDTDNE---EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEE 122

Query: 414 XXXXIQEIDTDGSGTVDFDEFMEMMTGE 497
               I+E DTDG G ++++EF  +++ +
Sbjct: 123 VADMIREADTDGDGVINYEEFSRVISSK 150


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 60.1 bits (139), Expect = 3e-10
 Identities = 38/149 (25%), Positives = 68/149 (45%)
 Frame = +3

Query: 51  ELPPEQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGR 230
           E+  EQ   + +AF  FD ++  +I    +   +R +G    K  + +++ + D    G 
Sbjct: 30  EITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGY 89

Query: 231 LEFPEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXX 410
           L+  +FV +  + IVE D     +E++ AF L+D +  G I   +LR +           
Sbjct: 90  LQMEDFVRVMTEKIVERDP---LEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQ 146

Query: 411 XXXXXIQEIDTDGSGTVDFDEFMEMMTGE 497
                I+E D D  G ++  EF+ +M  E
Sbjct: 147 ELEAMIEEFDLDQDGEINEQEFIAIMMDE 175



 Score = 39.1 bits (87), Expect = 7e-04
 Identities = 21/75 (28%), Positives = 40/75 (53%)
 Frame = +3

Query: 33  TEDWADELPPEQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVD 212
           TE   +  P E+I   ++AF+ FD + +G I    +  + + + +  + + LE +IEE D
Sbjct: 100 TEKIVERDPLEEI---KRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFD 156

Query: 213 ADKSGRLEFPEFVTL 257
            D+ G +   EF+ +
Sbjct: 157 LDQDGEINEQEFIAI 171


>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 174

 Score = 59.3 bits (137), Expect = 6e-10
 Identities = 42/149 (28%), Positives = 68/149 (45%), Gaps = 5/149 (3%)
 Frame = +3

Query: 63  EQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFP 242
           E+I  +RK F   D N+SGSI  +    I  +   P    +   L   VD D  G ++F 
Sbjct: 21  EEIERIRKRFIKIDANQSGSIDRNEFLSIPSVASNP----LASRLFSVVDEDGGGDVDFQ 76

Query: 243 EFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSL-----REIXXXXXXXXXX 407
           EF+   + F V  + E   ++L+ AF++YD + +GYI    L       +          
Sbjct: 77  EFINSLSVFSVHGNKE---EKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQ 133

Query: 408 XXXXXXIQEIDTDGSGTVDFDEFMEMMTG 494
                 I E+D D  G + F+EF ++++G
Sbjct: 134 QIVDKTIMEVDKDRDGKISFEEFKDIVSG 162


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 55.6 bits (128), Expect = 7e-09
 Identities = 35/140 (25%), Positives = 68/140 (48%)
 Frame = +3

Query: 63  EQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFP 242
           EQ   +++AF  +D ++ G IP   V  +LR +G       L +L  E+       ++  
Sbjct: 6   EQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL----GDAIDEK 61

Query: 243 EFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXXXXXX 422
           +F++  +  + E ++E   +E  +AFR++DK+ +GYI T+   +                
Sbjct: 62  KFMSFVSNKLRETESE---EEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQL 118

Query: 423 XIQEIDTDGSGTVDFDEFME 482
            +QE D   SG+ D+ +F++
Sbjct: 119 MVQEADPTNSGSFDYYDFVQ 138


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 40.7 bits (91), Expect = 2e-04
 Identities = 30/136 (22%), Positives = 62/136 (45%)
 Frame = +3

Query: 81  RKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFPEFVTLA 260
           ++AF  FD + +G IP   + D+LR  GQ  N  + E  I E+++     ++  +F+ + 
Sbjct: 9   KQAFSLFDRHGTGRIPKTSIGDLLRACGQ--NPTLAE--ITEIESTLPAEVDMEQFLQVL 64

Query: 261 AKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXXXXXXXIQEID 440
            +     D     +E  + F+++DK+  G I    LR +                ++ + 
Sbjct: 65  NR-PNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVP 123

Query: 441 TDGSGTVDFDEFMEMM 488
               G V++ +F++M+
Sbjct: 124 VK-DGMVNYHDFVQMI 138


>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
           Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 190

 Score = 33.1 bits (72), Expect = 0.043
 Identities = 21/63 (33%), Positives = 32/63 (50%)
 Frame = +3

Query: 189 EELIEEVDADKSGRLEFPEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSL 368
           E +    DADK+G ++F EF+   +   V    E +  +L  AF+LYD + NG I    +
Sbjct: 66  EYVFNVFDADKNGYIDFKEFICALS---VTSRGE-LNDKLIWAFQLYDLDNNGLISYDEM 121

Query: 369 REI 377
             I
Sbjct: 122 LRI 124


>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
           Plc1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 899

 Score = 31.1 bits (67), Expect = 0.17
 Identities = 15/53 (28%), Positives = 28/53 (52%)
 Frame = +3

Query: 90  FDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFPEF 248
           ++  +  +S  +    V  + +M+    + + LEE  ++ DAD SG+L F EF
Sbjct: 303 WEKLEKEQSAQLDLGDVHRMCQMLHLNASMEFLEETFQKADADHSGKLSFEEF 355


>SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 2280

 Score = 29.5 bits (63), Expect = 0.53
 Identities = 19/65 (29%), Positives = 31/65 (47%), Gaps = 1/65 (1%)
 Frame = -3

Query: 476  ELVEVDGAASVSV-DLLDEAIEFIVSQLLVQFPEDLAQARRGNVTVAFLVVQSERFPEFF 300
            EL  V   A  S  DL D AI   ++Q+L  F +DL +     VT+    +    +P ++
Sbjct: 1246 ELTNVVNVALTSTGDLDDSAIVSKLNQILSDFRDDLLEHNVRRVTIVGGRINKSAYPSYY 1305

Query: 299  LHGLS 285
             + +S
Sbjct: 1306 TYRVS 1310


>SPAC926.03 |rlc1||myosin II regulatory light chain
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 184

 Score = 27.1 bits (57), Expect = 2.8
 Identities = 11/18 (61%), Positives = 15/18 (83%)
 Frame = +3

Query: 300 KELREAFRLYDKEGNGYI 353
           +EL+EAF L DK+G+G I
Sbjct: 48  QELKEAFALLDKDGDGNI 65


>SPAC1687.14c |||EF hand family protein, unknown
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 76

 Score = 25.8 bits (54), Expect = 6.5
 Identities = 12/26 (46%), Positives = 14/26 (53%)
 Frame = +3

Query: 294 MQKELREAFRLYDKEGNGYIPTSSLR 371
           M +E  EAF L+D    GYI    LR
Sbjct: 10  MDEEAEEAFDLFDVTHKGYIDFEDLR 35


>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1236

 Score = 25.8 bits (54), Expect = 6.5
 Identities = 15/36 (41%), Positives = 21/36 (58%)
 Frame = +1

Query: 370  ARSSGNWTSS*LTMNSMASSKRSTLTEAAPSTSTSS 477
            A ++G+ TSS  T +   SS  S  T +A  +STSS
Sbjct: 968  ASATGSSTSSIATASVSGSSTSSVATASATDSSTSS 1003



 Score = 25.4 bits (53), Expect = 8.6
 Identities = 16/38 (42%), Positives = 21/38 (55%)
 Frame = +1

Query: 364  ACARSSGNWTSS*LTMNSMASSKRSTLTEAAPSTSTSS 477
            A A  +G+ TSS  T +   SS  S  T +A  +STSS
Sbjct: 1005 AAASVTGSSTSSVATASVTDSSTSSVATASATDSSTSS 1042


>SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 614

 Score = 25.4 bits (53), Expect = 8.6
 Identities = 11/22 (50%), Positives = 15/22 (68%)
 Frame = +3

Query: 186 LEELIEEVDADKSGRLEFPEFV 251
           + E I EV+ D SGR+E  +FV
Sbjct: 56  VREAIREVNVDSSGRVEPEDFV 77


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,553,175
Number of Sequences: 5004
Number of extensions: 45704
Number of successful extensions: 185
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 353266144
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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