BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30561 (509 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 322 2e-89 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 192 3e-50 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 173 2e-44 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 126 2e-30 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 77 2e-15 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 76 3e-15 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 66 4e-12 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 65 7e-12 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 54 9e-09 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 37 0.002 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.18 SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 2.2 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 2.9 SPAC18B11.06 |||U3 snoRNP-associated protein Lcp5 |Schizosacchar... 25 6.6 SPCC970.03 |||cytochrome b5 reductase |Schizosaccharomyces pombe... 25 8.7 SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyc... 25 8.7 SPAC23H4.13c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 25 8.7 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 322 bits (791), Expect = 2e-89 Identities = 151/165 (91%), Positives = 160/165 (96%) Frame = +1 Query: 13 KIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLY 192 KIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQI+FETFN+PA YVAIQAVLSLY Sbjct: 84 KIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQAVLSLY 143 Query: 193 ASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTT 372 ASGRTTGIVLDSGDGV+HTVPIYEGYALPHAI+RLDLAGRDLTDYLMKIL ERGY+F+TT Sbjct: 144 ASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYTFSTT 203 Query: 373 AEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVI 507 AEREIVRDIKEKLCYVALDFEQE+ TAA S+SLEKSYELPDGQVI Sbjct: 204 AEREIVRDIKEKLCYVALDFEQELQTAAQSSSLEKSYELPDGQVI 248 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 192 bits (467), Expect = 3e-50 Identities = 86/165 (52%), Positives = 120/165 (72%) Frame = +1 Query: 13 KIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLY 192 ++W +TF+ +L++ P +LLTE P+NP ANREKM + MFE + +YVAIQAVLSLY Sbjct: 84 QLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETMFERYGFGGVYVAIQAVLSLY 143 Query: 193 ASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTT 372 A G ++G+V+DSGDGV+H VP+YE L H + RLD+AGRD T YL+ +L +GY+F T Sbjct: 144 AQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVGRLDVAGRDATRYLISLLLRKGYAFNRT 203 Query: 373 AEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVI 507 A+ E VR++KEKLCYV+ D E + + +T L ++Y LPDG+VI Sbjct: 204 ADFETVREMKEKLCYVSYDLELDHKLSEETTVLMRNYTLPDGRVI 248 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 173 bits (420), Expect = 2e-44 Identities = 80/166 (48%), Positives = 116/166 (69%), Gaps = 1/166 (0%) Frame = +1 Query: 13 KIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSL 189 +IW + + ++ L PEEHP+LLTE PL N+EK+ + +ET N PA+ ++Q VL+L Sbjct: 88 EIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQPVLAL 147 Query: 190 YASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTT 369 YAS RTTGIVL+ GDG++H+VPIY+G+++P AI + ++ GRD+TDYL L + G+ + Sbjct: 148 YASARTTGIVLECGDGLTHSVPIYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSGHELVS 207 Query: 370 TAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVI 507 +AE+EIVR+IKEK CYVA DF E+ + +Y+LPD Q I Sbjct: 208 SAEKEIVREIKEKCCYVASDFRSEIESWTEHKPQIHTYQLPDNQTI 253 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 126 bits (304), Expect = 2e-30 Identities = 68/155 (43%), Positives = 94/155 (60%), Gaps = 10/155 (6%) Frame = +1 Query: 13 KIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLY 192 + W + + LR PE+H LLTE PLNP NRE +IMFE+FN +Y+A+QAVL+L Sbjct: 107 RFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQAVLALA 166 Query: 193 ASGRT--------TGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTE 348 AS + TG V+DSGDGV+H +P+ EGY + +I + LAGRD+T ++ +L + Sbjct: 167 ASWTSSKVTDRSLTGTVVDSGDGVTHIIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRD 226 Query: 349 RGY--SFTTTAEREIVRDIKEKLCYVALDFEQEMA 447 R S TAER IKE+ CYV D +E + Sbjct: 227 RNEPDSSLKTAER-----IKEECCYVCPDIVKEFS 256 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 76.6 bits (180), Expect = 2e-15 Identities = 43/157 (27%), Positives = 79/157 (50%), Gaps = 2/157 (1%) Frame = +1 Query: 13 KIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLY 192 ++ +TF +L + EHP+ +TE NP R MT+++FE +N+P++ I + S Y Sbjct: 100 QVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFY 158 Query: 193 ASGR--TTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFT 366 + + ++GIVL+ G+ SH +P+ G + R+ G + YL+K+ + SF Sbjct: 159 HNTKPSSSGIVLNLGNAASHVIPVLNGERILSEAKRISWGGSQSSSYLLKLFQIKYPSFP 218 Query: 367 TTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEK 477 + C+V+ D+ ++A A LE+ Sbjct: 219 IKMLPSQAELLMHDHCHVSSDYTHDIAHALDRDILER 255 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 76.2 bits (179), Expect = 3e-15 Identities = 37/114 (32%), Positives = 59/114 (51%) Frame = +1 Query: 16 IWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYA 195 +W + +L+ P EHP+L+TE NP NR K + MFE+ PA Y+A Q + +A Sbjct: 83 LWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMFESLRCPATYLAKQETCAAFA 142 Query: 196 SGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGY 357 SG+ T ++D G S IY+G+ L +G + D L + L ++ + Sbjct: 143 SGKGTACLVDIGAERSSVSAIYDGFVLQKGYQVQHFSGNAINDILAQTLRDKNF 196 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 65.7 bits (153), Expect = 4e-12 Identities = 30/115 (26%), Positives = 59/115 (51%) Frame = +1 Query: 19 WHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYAS 198 W +L+V P E+ +++TE NP++ R+++ + FE + PA Y+ QAV +A+ Sbjct: 87 WERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAFYLTKQAVCVAFAN 146 Query: 199 GRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSF 363 ++T +++D G + P+ +G + I + LAG L + ++ F Sbjct: 147 SKSTALIVDIGSDNASVTPVVDGLIIRKGIFKQSLAGDFLNANIEQLFNTMNIEF 201 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 64.9 bits (151), Expect = 7e-12 Identities = 33/96 (34%), Positives = 57/96 (59%), Gaps = 2/96 (2%) Frame = +1 Query: 214 IVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVR 393 +V+DSG +H +P+ + A+ R+D+ G+ LT+YL ++++ R Y+ E IV Sbjct: 159 LVIDSGYSFTHIIPVIDFSVQEQAVKRVDVGGKLLTNYLKEVISYRKYNM--MEETYIVN 216 Query: 394 DIKEKLCYVALDFEQEMATA--AASTSLEKSYELPD 495 +IKE +CYV+ +F+++M + LE Y LPD Sbjct: 217 EIKESVCYVSQNFKEDMEICHEKPRSKLEICYALPD 252 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 54.4 bits (125), Expect = 9e-09 Identities = 39/133 (29%), Positives = 60/133 (45%), Gaps = 4/133 (3%) Frame = +1 Query: 19 WHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLS 186 W H Y+ L P + +PV L +RE TQ FE P +A + ++ Sbjct: 129 WKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFEECQVPGFTIAYEPLMG 187 Query: 187 LYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFT 366 LYA G G+V+D G + PI +G + A +L L GR +T +L +L E + Sbjct: 188 LYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLGGRYMTQHLQNLLRESLPTLK 247 Query: 367 TTAEREIVRDIKE 405 ++ + DI E Sbjct: 248 SSGQYVSKEDITE 260 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 36.7 bits (81), Expect = 0.002 Identities = 19/101 (18%), Positives = 48/101 (47%) Frame = +1 Query: 61 EHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGV 240 ++ V+ L + EK+ I+F + + +++ + + +G + V+D G Sbjct: 243 QYSVIFIVPDLYDRVYVEKILDILFFDLHFGKAAIVQESLCTSFGAGMSAACVVDMGAQK 302 Query: 241 SHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSF 363 + + EG +P++ ++++ G D+T MK+L + + Sbjct: 303 TSISCVEEGVVVPNSRIKINYGGDDITLLFMKLLMRSHFPY 343 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 30.3 bits (65), Expect = 0.18 Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%) Frame = +1 Query: 31 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 174 +Y L E+HP+LLT+ A L P+ + ++ +I ++ N+P + VAIQ Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451 >SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 248 Score = 26.6 bits (56), Expect = 2.2 Identities = 9/16 (56%), Positives = 9/16 (56%) Frame = +2 Query: 257 STKVTLCPTPSSVWTW 304 STK CP PS W W Sbjct: 52 STKTATCPDPSWEWAW 67 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 2.9 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +1 Query: 73 LLTEAPLNPKANREKMTQIMFETFN 147 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPAC18B11.06 |||U3 snoRNP-associated protein Lcp5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 327 Score = 25.0 bits (52), Expect = 6.6 Identities = 10/27 (37%), Positives = 15/27 (55%) Frame = +3 Query: 258 LRRLRSAPRHPPSGLGWSRLDRLPHED 338 L++ + +H G WS LDR H+D Sbjct: 254 LKKSKRVKKHDYGGEDWSLLDRKFHDD 280 >SPCC970.03 |||cytochrome b5 reductase |Schizosaccharomyces pombe|chr 3|||Manual Length = 301 Score = 24.6 bits (51), Expect = 8.7 Identities = 10/16 (62%), Positives = 11/16 (68%), Gaps = 1/16 (6%) Frame = +2 Query: 242 PTPYPSTKVTLC-PTP 286 P P P TKV +C PTP Sbjct: 256 PAPSPETKVLICGPTP 271 >SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyces pombe|chr 1|||Manual Length = 721 Score = 24.6 bits (51), Expect = 8.7 Identities = 7/18 (38%), Positives = 13/18 (72%) Frame = +1 Query: 226 SGDGVSHTVPIYEGYALP 279 +G G+ H + + +GYA+P Sbjct: 157 AGRGIGHQIMVEQGYAMP 174 >SPAC23H4.13c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual Length = 184 Score = 24.6 bits (51), Expect = 8.7 Identities = 16/36 (44%), Positives = 19/36 (52%) Frame = +1 Query: 397 IKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQV 504 IKEKL VA EQE A +SL+ S + G V Sbjct: 111 IKEKLRRVANITEQEQQCIPAESSLDLSIQAMKGVV 146 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,629,700 Number of Sequences: 5004 Number of extensions: 29500 Number of successful extensions: 123 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 114 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 119 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 204242806 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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