Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30555
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 291 2e-79
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 291 2e-79
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 291 2e-79
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 291 2e-79
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 173 4e-44
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 167 5e-42
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 100 5e-22
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 93 1e-19
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 56 1e-08
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 56 1e-08
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 52 2e-07
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 52 3e-07
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 50 9e-07
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 46 2e-05
At5g13650.2 68418.m01585 elongation factor family protein contai... 41 4e-04
At5g13650.1 68418.m01584 elongation factor family protein contai... 41 4e-04
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 40 0.001
At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 40 0.001
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 39 0.002
At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 38 0.004
At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 38 0.004
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 37 0.009
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 37 0.009
At4g11160.1 68417.m01808 translation initiation factor IF-2, mit... 33 0.15
At3g01360.1 68416.m00057 expressed protein contains Pfam profile... 30 0.80
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 30 0.80
At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 30 0.80
At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 30 1.1
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 30 1.1
At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 29 1.4
At4g20430.1 68417.m02981 subtilase family protein contains Pfam ... 27 7.5
At5g37055.1 68418.m04446 zinc finger (HIT type) family protein c... 27 9.9
At5g27540.1 68418.m03297 GTP-binding protein-related low similar... 27 9.9
At5g24280.1 68418.m02856 expressed protein ; expression supporte... 27 9.9
At5g20890.1 68418.m02481 chaperonin, putative similar to SWISS-P... 27 9.9
At4g14220.1 68417.m02194 zinc finger (C3HC4-type RING finger) fa... 27 9.9
At3g14080.2 68416.m01780 small nuclear ribonucleoprotein, putati... 27 9.9
At3g14080.1 68416.m01779 small nuclear ribonucleoprotein, putati... 27 9.9
At2g28000.1 68415.m03393 RuBisCO subunit binding-protein alpha s... 27 9.9
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 291 bits (714), Expect = 2e-79
Identities = 138/160 (86%), Positives = 146/160 (91%)
Frame = +1
Query: 37 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 216
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 217 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 396
DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 397 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEP 516
G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTP 160
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 291 bits (714), Expect = 2e-79
Identities = 138/160 (86%), Positives = 146/160 (91%)
Frame = +1
Query: 37 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 216
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 217 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 396
DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 397 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEP 516
G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTP 160
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 291 bits (714), Expect = 2e-79
Identities = 138/160 (86%), Positives = 146/160 (91%)
Frame = +1
Query: 37 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 216
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 217 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 396
DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 397 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEP 516
G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTP 160
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 291 bits (714), Expect = 2e-79
Identities = 138/160 (86%), Positives = 146/160 (91%)
Frame = +1
Query: 37 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 216
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 217 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 396
DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 397 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEP 516
G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTP 160
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 173 bits (422), Expect = 4e-44
Identities = 76/152 (50%), Positives = 110/152 (72%), Gaps = 1/152 (0%)
Frame = +1
Query: 52 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKA 231
+ +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297
Query: 232 ERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEA 411
ERERGIT+ +A+ F + +++V ++D+PGH+DF+ NMI G +QAD A+L++ A G FEA
Sbjct: 298 ERERGITMTVAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEA 357
Query: 412 GISK-NGQTREHALLAFTLGVKQLIVGVNKMD 504
G GQTREHA + GV+Q+IV +NKMD
Sbjct: 358 GFDNLKGQTREHARVLRGFGVEQVIVAINKMD 389
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 167 bits (405), Expect = 5e-42
Identities = 76/153 (49%), Positives = 108/153 (70%)
Frame = +1
Query: 46 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 225
+K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA++ + S+ A+++D
Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157
Query: 226 KAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEF 405
+ ER +G T+++ FET TI+DAPGH+ ++ NMI+G SQAD VL+++A GEF
Sbjct: 158 EEERLKGKTVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKGEF 217
Query: 406 EAGISKNGQTREHALLAFTLGVKQLIVGVNKMD 504
E G + GQTREH LA TLGV +LIV VNKMD
Sbjct: 218 ETGYERGGQTREHVQLAKTLGVSKLIVVVNKMD 250
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 100 bits (240), Expect = 5e-22
Identities = 58/160 (36%), Positives = 84/160 (52%)
Frame = +1
Query: 34 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWV 213
K ++K H+NI IGHVD GK+T T L I +K+++
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE--------------- 116
Query: 214 LDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 393
+D ER RGITI+ A ++ET + +D PGH D++KNMITG +Q D A+L+V+
Sbjct: 117 IDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 176
Query: 394 TGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTE 513
G QT+EH LLA +GV ++V +NK D +
Sbjct: 177 DGPMP-------QTKEHILLAKQVGVPDMVVFLNKEDQVD 209
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 93.1 bits (221), Expect = 1e-19
Identities = 57/154 (37%), Positives = 81/154 (52%)
Frame = +1
Query: 43 KEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 222
+ K H+N+ IGHVD GK+T T + K E GK +DK
Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLTAAIT---------------KVLAEEGKAKAIAFDEIDK 107
Query: 223 LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGE 402
E++RGITI A ++ET+K + +D PGH D++KNMITG +Q D +L+V+ G
Sbjct: 108 APEEKKRGITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGP 167
Query: 403 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD 504
QT+EH LLA +GV L+ +NK+D
Sbjct: 168 MP-------QTKEHILLARQVGVPSLVCFLNKVD 194
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 56.4 bits (130), Expect = 1e-08
Identities = 41/128 (32%), Positives = 61/128 (47%)
Frame = +1
Query: 61 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 240
NI + H+DSGK+T T +++ G I E E ++ G G+ +D + ERE
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLERE 116
Query: 241 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 420
+GITI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176
Query: 421 KNGQTREH 444
+ Q R +
Sbjct: 177 VDRQMRRY 184
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 56.4 bits (130), Expect = 1e-08
Identities = 41/128 (32%), Positives = 61/128 (47%)
Frame = +1
Query: 61 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 240
NI + H+DSGK+T T +++ G I E E ++ G G+ +D + ERE
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLERE 116
Query: 241 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 420
+GITI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176
Query: 421 KNGQTREH 444
+ Q R +
Sbjct: 177 VDRQMRRY 184
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 52.0 bits (119), Expect = 2e-07
Identities = 36/113 (31%), Positives = 53/113 (46%)
Frame = +1
Query: 61 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 240
NI ++ HVD GK+T HLI GG + GK F +D L E+
Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG--------GVLHPRLAGKLRF-----MDYLDEEQR 57
Query: 241 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 399
R IT+ + + Y + +ID+PGH DF + T +D A+++V A G
Sbjct: 58 RAITMKSSSISLKYKDYSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEG 110
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 51.6 bits (118), Expect = 3e-07
Identities = 36/117 (30%), Positives = 56/117 (47%)
Frame = +1
Query: 49 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 228
K + NI ++ H+D+GK+TTT ++Y G K E+ +G+ W ++
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTGRNYK---------IGEVHEGTATMDW----ME 140
Query: 229 AERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 399
E+ERGITI A K+ + IID PGH DF + D A+ + + G
Sbjct: 141 QEQERGITITSAATTTFWDKHRINIIDTPGHVDFTLEVERALRVLDGAICLFDSVAG 197
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 50.0 bits (114), Expect = 9e-07
Identities = 36/117 (30%), Positives = 54/117 (46%), Gaps = 4/117 (3%)
Frame = +1
Query: 61 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 240
N +I H+D GKST L+ G + R + K F LD + ERE
Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDM-----------KEQF-----LDNMDLERE 131
Query: 241 RGITIDIAL----WKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 399
RGITI + + +E + + + +ID PGH DF + + + A+L+V A G
Sbjct: 132 RGITIKLQAARMRYVYEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQG 188
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 45.6 bits (103), Expect = 2e-05
Identities = 53/169 (31%), Positives = 70/169 (41%), Gaps = 9/169 (5%)
Frame = +1
Query: 25 D*PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKY 204
D K EK N +I H+D GKST L+ G I K G G +Y
Sbjct: 57 DLTKFPSEKIR-NFSIIAHIDHGKSTLADRLMELTGTIKK-------------GHGQPQY 102
Query: 205 AWVLDKLKAERERGITIDIALWKF---------ETSKYYVTIIDAPGHRDFIKNMITGTS 357
LDKL +RERGIT+ E S Y + +ID PGH DF + S
Sbjct: 103 ---LDKL--QRERGITVKAQTATMFYENKVEDQEASGYLLNLIDTPGHVDFSYEVSRSLS 157
Query: 358 QADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD 504
A+L+V A G QT + LAF + ++ +NK+D
Sbjct: 158 ACQGALLVVDAAQG-------VQAQTVANFYLAFEANL-TIVPVINKID 198
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 41.1 bits (92), Expect = 4e-04
Identities = 43/148 (29%), Positives = 62/148 (41%)
Frame = +1
Query: 61 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 240
NI ++ HVD GK+T ++ + K ++ Q M + ++D ERE
Sbjct: 85 NIAIVAHVDHGKTTLVDSMLRQA---------KVFRDNQVMQER------IMDSNDLERE 129
Query: 241 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 420
RGITI V IID PGH DF + + D +L+V + G
Sbjct: 130 RGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEGPMP---- 185
Query: 421 KNGQTREHALLAFTLGVKQLIVGVNKMD 504
QTR A G ++V VNK+D
Sbjct: 186 ---QTRFVLKKALEFG-HAVVVVVNKID 209
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 41.1 bits (92), Expect = 4e-04
Identities = 43/148 (29%), Positives = 62/148 (41%)
Frame = +1
Query: 61 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 240
NI ++ HVD GK+T ++ + K ++ Q M + ++D ERE
Sbjct: 84 NIAIVAHVDHGKTTLVDSMLRQA---------KVFRDNQVMQER------IMDSNDLERE 128
Query: 241 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 420
RGITI V IID PGH DF + + D +L+V + G
Sbjct: 129 RGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEGPMP---- 184
Query: 421 KNGQTREHALLAFTLGVKQLIVGVNKMD 504
QTR A G ++V VNK+D
Sbjct: 185 ---QTRFVLKKALEFG-HAVVVVVNKID 208
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 39.5 bits (88), Expect = 0.001
Identities = 45/172 (26%), Positives = 76/172 (44%), Gaps = 14/172 (8%)
Frame = +1
Query: 31 PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK-RTIEKFEKEAQ-EMGKGSFK- 201
P++ + INI IGHV GKST ++ G+ R + E+ ++G + K
Sbjct: 31 PEVISRQATINIGTIGHVAHGKST----IVKAISGVQTVRFKNELERNITIKLGYANAKI 86
Query: 202 YAWVLDKLK---------AERERGITIDIALWKF--ETSKYYVTIIDAPGHRDFIKNMIT 348
Y DK + +E T D+ ++ T + +V+ +D PGH + M+
Sbjct: 87 YKCEDDKCPRPMCYKSYGSGKEDNPTCDVPGFENCKMTLRRHVSCVDCPGHDILMATMLN 146
Query: 349 GTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD 504
G + D A+L++AA QT EH + +K +I+ NK+D
Sbjct: 147 GAAIVDGALLLIAANES------CPQPQTAEHLASVDMMRLKHIIILQNKID 192
>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 284
Score = 39.5 bits (88), Expect = 0.001
Identities = 45/172 (26%), Positives = 76/172 (44%), Gaps = 14/172 (8%)
Frame = +1
Query: 31 PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK-RTIEKFEKEAQ-EMGKGSFK- 201
P++ + INI IGHV GKST ++ G+ R + E+ ++G + K
Sbjct: 31 PEVISRQATINIGTIGHVAHGKST----IVKAISGVQTVRFKNELERNITIKLGYANAKI 86
Query: 202 YAWVLDKLK---------AERERGITIDIALWKF--ETSKYYVTIIDAPGHRDFIKNMIT 348
Y DK + +E T D+ ++ T + +V+ +D PGH + M+
Sbjct: 87 YKCEDDKCPRPMCYKSYGSGKEDNPTCDVPGFENCKMTLRRHVSCVDCPGHDILMATMLN 146
Query: 349 GTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD 504
G + D A+L++AA QT EH + +K +I+ NK+D
Sbjct: 147 GAAIVDGALLLIAANES------CPQPQTAEHLASVDMMRLKHIIILQNKID 192
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 39.1 bits (87), Expect = 0.002
Identities = 30/112 (26%), Positives = 51/112 (45%), Gaps = 4/112 (3%)
Frame = +1
Query: 61 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRT---IEKFEKEAQEMGKG-SFKYAWVLDKLK 228
N+ VI HVD GKST T L+ G I + + + A E +G + K + +
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTRADEAERGITIKSTGISLYYE 80
Query: 229 AERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 384
E ++ + ++Y + +ID+PGH DF + D A+++V
Sbjct: 81 MTDE---SLKSFTGARDGNEYLINLIDSPGHVDFSSEVTAALRITDGALVVV 129
>At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative
Length = 465
Score = 37.9 bits (84), Expect = 0.004
Identities = 42/166 (25%), Positives = 71/166 (42%), Gaps = 8/166 (4%)
Frame = +1
Query: 31 PKMGKEKTHINIVVIGHVDSGKST----TTG-HLIYKCGGIDKR-TIEKFEKEAQEMGKG 192
P++ + INI IGHV GKST +G H + +++ TI+ A+
Sbjct: 25 PEVISRQATINIGTIGHVAHGKSTIVKAVSGVHTVKFKSELERNITIKLGYANAKIYKCE 84
Query: 193 SFKYAWVLDKLKAERERGITIDIALWKFETSKY--YVTIIDAPGHRDFIKNMITGTSQAD 366
+ +E + D++ + K +V+ +D PGH + M+ G + D
Sbjct: 85 KCPRPVAYKSFGSGKEDNPSCDVSGHEKYKMKLLRHVSFVDCPGHDILMATMLNGAAIMD 144
Query: 367 CAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD 504
A+LI+AA QT EH + +K +I+ NK+D
Sbjct: 145 GALLIIAANE------TCPQPQTAEHLASVDMMHLKDIIIIQNKID 184
>At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to gb|U37354 from S. pombe. ESTs
gb|T41979, gb|N37284 and gb|N37529 come from this gene
Length = 465
Score = 37.9 bits (84), Expect = 0.004
Identities = 25/81 (30%), Positives = 39/81 (48%), Gaps = 4/81 (4%)
Frame = +1
Query: 274 FETSKY----YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTRE 441
FE SK +V+ +D PGH + M+ G + D A+L++AA QT E
Sbjct: 112 FENSKMKLLRHVSFVDCPGHDILMATMLNGAAIMDGALLLIAANE------TCPQPQTSE 165
Query: 442 HALLAFTLGVKQLIVGVNKMD 504
H + +K +I+ NK+D
Sbjct: 166 HLAAVEIMQLKHIIILQNKID 186
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 36.7 bits (81), Expect = 0.009
Identities = 35/118 (29%), Positives = 49/118 (41%), Gaps = 5/118 (4%)
Frame = +1
Query: 61 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 240
N+ ++GH+ GK+ L+ + + K EK KY D E+E
Sbjct: 140 NVALVGHLQHGKTVFMDMLVEQTHHMSTFNA-KNEKH--------MKYT---DTRVDEQE 187
Query: 241 RGITI-----DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 399
R I+I + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 188 RNISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 36.7 bits (81), Expect = 0.009
Identities = 35/118 (29%), Positives = 49/118 (41%), Gaps = 5/118 (4%)
Frame = +1
Query: 61 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 240
N+ ++GH+ GK+ L+ + + K EK KY D E+E
Sbjct: 140 NVALVGHLQHGKTVFMDMLVEQTHHMSTFNA-KNEKH--------MKYT---DTRVDEQE 187
Query: 241 RGITI-----DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 399
R I+I + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 188 RNISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At4g11160.1 68417.m01808 translation initiation factor IF-2,
mitochondrial, putative similar to SP|P46198|IF2M_BOVIN
Translation initiation factor IF-2, mitochondrial
precursor (IF-2Mt) (IF-2(Mt)) {Bos taurus}
Length = 743
Score = 32.7 bits (71), Expect = 0.15
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +1
Query: 295 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 399
+T +D PGH F + G + D VL+VAA G
Sbjct: 270 ITFLDTPGHAAFSEMRARGAAVTDIVVLVVAADDG 304
>At3g01360.1 68416.m00057 expressed protein contains Pfam profile
PF04819: Family of unknown function (DUF716) (Plant
viral-response family)
Length = 319
Score = 30.3 bits (65), Expect = 0.80
Identities = 21/71 (29%), Positives = 31/71 (43%), Gaps = 1/71 (1%)
Frame = +1
Query: 304 IDAPGHRDFIKNMITGTSQADCAV-LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 480
I GH D+ + T Q +C + L+V TG F +KNG R+ LG +
Sbjct: 226 IKCKGHGDYHRAKAIATLQFNCHLALMVVVATGLFSVIANKNGYLRQDHSKYRPLGAELE 285
Query: 481 IVGVNKMDSTE 513
+ +DS E
Sbjct: 286 NLSTFTLDSDE 296
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 30.3 bits (65), Expect = 0.80
Identities = 21/68 (30%), Positives = 34/68 (50%)
Frame = +1
Query: 301 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 480
+ID PGH F G+S D A+L+V + + G+ QT E +L + +
Sbjct: 705 VIDTPGHESFTNLRSRGSSLCDLAILVV-----DIKHGLEP--QTIE-SLNLLRMRNTEF 756
Query: 481 IVGVNKMD 504
I+ +NK+D
Sbjct: 757 IIALNKVD 764
>At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1088
Score = 30.3 bits (65), Expect = 0.80
Identities = 22/70 (31%), Positives = 33/70 (47%)
Frame = +1
Query: 295 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 474
+ +ID PGH F G++ D A+L+V + G+ QT E L VK
Sbjct: 558 ILVIDTPGHESFTNLRSRGSNLCDLAILVV-----DIMRGLEP--QTIESLNLLRRRNVK 610
Query: 475 QLIVGVNKMD 504
I+ +NK+D
Sbjct: 611 -FIIALNKVD 619
>At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profile PF00009: Elongation
factor Tu GTP binding domain
Length = 630
Score = 29.9 bits (64), Expect = 1.1
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +1
Query: 301 IIDAPGHRDFIKNMITGTSQADCAVLIV 384
+ID PGH F G+S D A+L+V
Sbjct: 113 VIDTPGHESFTNLRSRGSSLCDLAILVV 140
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 29.9 bits (64), Expect = 1.1
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +1
Query: 301 IIDAPGHRDFIKNMITGTSQADCAVLIV 384
+ID PGH F G+S D A+L+V
Sbjct: 772 VIDTPGHESFTNLRSRGSSLCDLAILVV 799
>At1g17220.1 68414.m02098 translation initiation factor IF-2,
chloroplast, putative similar to SP|P57997|IF2C_PHAVU
Translation initiation factor IF-2, chloroplast
precursor (PvIF2cp) {Phaseolus vulgaris}
Length = 1026
Score = 29.5 bits (63), Expect = 1.4
Identities = 26/99 (26%), Positives = 38/99 (38%), Gaps = 4/99 (4%)
Frame = +1
Query: 220 KLKAERERGITIDIALWKFET----SKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 387
K+ A GIT I +K +D PGH F G D A+++VA
Sbjct: 525 KVAASEAGGITQGIGAYKVSVPVDGKLQSCVFLDTPGHEAFGAMRARGARVTDIAIIVVA 584
Query: 388 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD 504
A G QT E A+ +++ +NK+D
Sbjct: 585 ADDG-------IRPQTNE-AIAHAKAAAVPIVIAINKID 615
>At4g20430.1 68417.m02981 subtilase family protein contains Pfam
profile: PF00082 subtilase family
Length = 856
Score = 27.1 bits (57), Expect = 7.5
Identities = 20/58 (34%), Positives = 27/58 (46%)
Frame = -1
Query: 477 LFDTEGESEQGMLTGLTVLRDTSFEFTGTGSYDEHSAISLRGSCDHVLDEISVSRSIN 304
+FDT E L G+ F +TGT ++ IS GS D L I+VS+ N
Sbjct: 714 IFDTSFEDYMSFLCGINGSAPVVFNYTGTNCLRNNATIS--GS-DLNLPSITVSKLNN 768
>At5g37055.1 68418.m04446 zinc finger (HIT type) family protein
contains Pfam profile: PF04438 HIT zinc finger
Length = 171
Score = 26.6 bits (56), Expect = 9.9
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = -3
Query: 214 IPKHI*RILYPFPGPPSRTSRWYVC 140
+P H+ L GPPS +SR Y C
Sbjct: 110 LPSHVPTYLKAAVGPPSSSSRRYFC 134
>At5g27540.1 68418.m03297 GTP-binding protein-related low similarity
to Mig-2-like GTPase Mtl [Drosophila melanogaster]
GI:7271872; contains Pfam profile PF00036: EF hand
Length = 648
Score = 26.6 bits (56), Expect = 9.9
Identities = 12/45 (26%), Positives = 21/45 (46%)
Frame = +1
Query: 283 SKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGI 417
++Y +D PG ++ ++ G + LIVAA T F +
Sbjct: 2 ARYAAGAVDCPGSPKSVRIVVVGDKGTGKSSLIVAAATDSFPPNV 46
>At5g24280.1 68418.m02856 expressed protein ; expression supported
by MPSS
Length = 1634
Score = 26.6 bits (56), Expect = 9.9
Identities = 15/42 (35%), Positives = 23/42 (54%)
Frame = -2
Query: 449 KACSRV*PFLEIPASNSPVPAATMSTAQSA*EVPVIMFLMKS 324
K+C RV F+++ A SP P+ T +Q+ V + F KS
Sbjct: 507 KSCRRVKCFVDLDAGFSPTPSKTDLASQNPFSVALRNFGSKS 548
>At5g20890.1 68418.m02481 chaperonin, putative similar to
SWISS-PROT:P78371- T-complex protein 1, beta subunit
(TCP-1-beta) [Homo sapiens]; contains Pfam:PF00118
domain, TCP-1/cpn60 chaperonin family
Length = 527
Score = 26.6 bits (56), Expect = 9.9
Identities = 17/54 (31%), Positives = 28/54 (51%)
Frame = -1
Query: 444 MLTGLTVLRDTSFEFTGTGSYDEHSAISLRGSCDHVLDEISVSRSINDGNIVLA 283
++ + + D F+G + +I LRG+ HVLDE RS++D VL+
Sbjct: 343 LIEEIMIGEDKLIHFSGC-EMGQACSIVLRGASHHVLDE--AERSLHDALCVLS 393
>At4g14220.1 68417.m02194 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 371
Score = 26.6 bits (56), Expect = 9.9
Identities = 14/39 (35%), Positives = 23/39 (58%)
Frame = +1
Query: 382 VAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 498
+AA + +++ ISK+ Q + LLA VK+L GV +
Sbjct: 261 LAAASAKYKESISKSKQGLKEKLLARNNSVKELSKGVQR 299
>At3g14080.2 68416.m01780 small nuclear ribonucleoprotein, putative
/ snRNP, putative / Sm protein, putative similar to U6
snRNA-associated Sm-like protein LSm1 (Small nuclear
ribonuclear CaSm, Cancer-associated Sm-like) [Homo
sapiens] SWISS-PROT:O15116; contains Pfam profile:
PF01423 Sm protein
Length = 128
Score = 26.6 bits (56), Expect = 9.9
Identities = 12/41 (29%), Positives = 24/41 (58%)
Frame = +1
Query: 61 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEM 183
N+V+IG +D+ + H+I + + + +K E+EA E+
Sbjct: 75 NVVLIGELDTEREELPPHMI-RVSEAEIKRAQKVEREASEL 114
>At3g14080.1 68416.m01779 small nuclear ribonucleoprotein, putative
/ snRNP, putative / Sm protein, putative similar to U6
snRNA-associated Sm-like protein LSm1 (Small nuclear
ribonuclear CaSm, Cancer-associated Sm-like) [Homo
sapiens] SWISS-PROT:O15116; contains Pfam profile:
PF01423 Sm protein
Length = 128
Score = 26.6 bits (56), Expect = 9.9
Identities = 12/41 (29%), Positives = 24/41 (58%)
Frame = +1
Query: 61 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEM 183
N+V+IG +D+ + H+I + + + +K E+EA E+
Sbjct: 75 NVVLIGELDTEREELPPHMI-RVSEAEIKRAQKVEREASEL 114
>At2g28000.1 68415.m03393 RuBisCO subunit binding-protein alpha
subunit, chloroplast / 60 kDa chaperonin alpha subunit /
CPN-60 alpha identical to SWISS-PROT:P21238- RuBisCO
subunit binding-protein alpha subunit, chloroplast
precursor (60 kDa chaperonin alpha subunit, CPN-60
alpha) [Arabidopsis thaliana]
Length = 586
Score = 26.6 bits (56), Expect = 9.9
Identities = 11/41 (26%), Positives = 22/41 (53%)
Frame = -1
Query: 387 SYDEHSAISLRGSCDHVLDEISVSRSINDGNIVLASFELPE 265
++D+HS +L+ D + D + ++ N+VL F P+
Sbjct: 52 AFDQHSRAALQAGIDKLADCVGLTLGPRGRNVVLDEFGSPK 92
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,757,593
Number of Sequences: 28952
Number of extensions: 212650
Number of successful extensions: 648
Number of sequences better than 10.0: 39
Number of HSP's better than 10.0 without gapping: 610
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 634
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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