SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30528
         (508 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC8C9.03 |cgs1||cAMP-dependent protein kinase regulatory subun...    81   1e-16
SPAC3G6.01 |hrp3||ATP-dependent DNA helicase Hrp3|Schizosaccharo...    27   1.2  
SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe...    27   1.6  
SPCP1E11.02 |ppk38||Ark1/Prk1 family protein kinase Ppk38|Schizo...    27   2.1  
SPAC637.13c |||cytoskeletal signaling protein|Schizosaccharomyce...    27   2.1  
SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3 ...    26   2.8  
SPAPJ698.03c |prp12|sap130|U2 snRNP-associated protein Sap130 |S...    26   2.8  
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M...    26   2.8  
SPBC713.09 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   3.7  
SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22...    26   3.7  
SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |S...    26   3.7  
SPAC11H11.04 |mam2||pheromone p-factor receptor|Schizosaccharomy...    25   4.9  
SPAC6F12.05c |tnr3||thiamine diphosphokinase Tnr3 |Schizosacchar...    25   6.5  
SPCC584.05 |sec1||SNARE binding protein Sec1|Schizosaccharomyces...    25   6.5  
SPBC1539.10 |||ribosome biogenesis protein Nop16 |Schizosaccharo...    25   8.6  
SPAC2G11.06 |vps4||AAA family ATPase Vps4|Schizosaccharomyces po...    25   8.6  

>SPAC8C9.03 |cgs1||cAMP-dependent protein kinase regulatory subunit
           Cgs1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 412

 Score = 80.6 bits (190), Expect = 1e-16
 Identities = 57/143 (39%), Positives = 77/143 (53%), Gaps = 12/143 (8%)
 Frame = +2

Query: 44  RRKSVFAETYDPEEDDSDEGAPAVFPKSDAQRA-RLAEAVRGILLFRSLDAQQMQQVLDA 220
           RR+SV  E+ +P     +      FP  D +   RL  +V G  LF++LD +   +VL+A
Sbjct: 101 RRQSVSTESMNPSAFALE--TKRTFPPKDPEDLKRLKRSVAGNFLFKNLDEEHYNEVLNA 158

Query: 221 MFEKR-SEPGEYVIRQGDDGDNFYVIENGVFDVL------VTGDDRVE----KVVHTYEG 367
           M EKR  E G  VI QG  GD FY++E G FDV       +T ++ +       + T   
Sbjct: 159 MTEKRIGEAGVAVIVQGAVGDYFYIVEQGEFDVYKRPELNITPEEVLSSGYGNYITTISP 218

Query: 368 SGSFGELALMYNMPRAASVRAQT 436
              FGELALMYN PRAASV ++T
Sbjct: 219 GEYFGELALMYNAPRAASVVSKT 241



 Score = 64.1 bits (149), Expect = 1e-11
 Identities = 40/108 (37%), Positives = 59/108 (54%)
 Frame = +2

Query: 113 VFPKSDAQRARLAEAVRGILLFRSLDAQQMQQVLDAMFEKRSEPGEYVIRQGDDGDNFYV 292
           VF  +  QR      +  + +  SLD  Q Q++ DA+     + G  VIRQGD G+ FY+
Sbjct: 258 VFENAYRQRMLYESLLEEVPILSSLDKYQRQKIADALQTVVYQAGSIVIRQGDIGNQFYL 317

Query: 293 IENGVFDVLVTGDDRVEKVVHTYEGSGSFGELALMYNMPRAASVRAQT 436
           IE+G  +V+  G    + VV T      FGELAL++   R A+V+A+T
Sbjct: 318 IEDGEAEVVKNG----KGVVVTLTKGDYFGELALIHETVRNATVQAKT 361


>SPAC3G6.01 |hrp3||ATP-dependent DNA helicase
           Hrp3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1388

 Score = 27.5 bits (58), Expect = 1.2
 Identities = 17/63 (26%), Positives = 31/63 (49%)
 Frame = +2

Query: 62  AETYDPEEDDSDEGAPAVFPKSDAQRARLAEAVRGILLFRSLDAQQMQQVLDAMFEKRSE 241
           AE+   EE +SD+ +    PK  +++ +    V+  + F S +++ +    DA FE   E
Sbjct: 111 AESVTSEESESDDSSYGGTPKKRSRQKKSNTYVQDEIRFSSRNSKGVNYNEDAYFESFEE 170

Query: 242 PGE 250
             E
Sbjct: 171 EEE 173


>SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 317

 Score = 27.1 bits (57), Expect = 1.6
 Identities = 24/91 (26%), Positives = 41/91 (45%), Gaps = 3/91 (3%)
 Frame = -2

Query: 363 SYVWTTFSTRSSPV---TRTSKTPFSMT*KLSPSSPCLITYSPGSDLFSNIASRTCCICC 193
           SY  T  ST  +P    TRTS + F  +   +PSS    + S  S   S  +S +     
Sbjct: 122 SYSGTISSTSIAPSMIGTRTSSSYFITSSSSTPSSSSSSSSSSPSSSSSKSSSSSKSSSS 181

Query: 192 ASRERNSSMPRTASARRALCASDLGNTAGAP 100
           +S    SS   ++S++ +  +S    ++ +P
Sbjct: 182 SSSSSKSSSSSSSSSKSSSSSSSSSKSSASP 212


>SPCP1E11.02 |ppk38||Ark1/Prk1 family protein kinase
           Ppk38|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 650

 Score = 26.6 bits (56), Expect = 2.1
 Identities = 15/46 (32%), Positives = 24/46 (52%), Gaps = 2/46 (4%)
 Frame = -2

Query: 339 TRSSPVTRT--SKTPFSMT*KLSPSSPCLITYSPGSDLFSNIASRT 208
           T+ +P + T     P     K+SP++P L T +   D+FS+ A  T
Sbjct: 416 TQGAPPSHTYGPPPPVQPKPKISPTTPRLSTLALADDMFSSTAKET 461


>SPAC637.13c |||cytoskeletal signaling protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 498

 Score = 26.6 bits (56), Expect = 2.1
 Identities = 12/49 (24%), Positives = 25/49 (51%)
 Frame = +2

Query: 74  DPEEDDSDEGAPAVFPKSDAQRARLAEAVRGILLFRSLDAQQMQQVLDA 220
           D ++DD ++  PAV  +S     R+++    +   RS  ++Q+    D+
Sbjct: 426 DDDDDDPNDFRPAVERQSSTMNTRMSQPSSAVNTNRSYGSEQIPSYADS 474


>SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 932

 Score = 26.2 bits (55), Expect = 2.8
 Identities = 19/65 (29%), Positives = 32/65 (49%), Gaps = 3/65 (4%)
 Frame = -2

Query: 333 SSPVTRTSKTPFSMT*KLSPS---SPCLITYSPGSDLFSNIASRTCCICCASRERNSSMP 163
           SSP+ R   TP S    ++PS    P     S G+   +++A+ +  +  +  ERN SM 
Sbjct: 459 SSPIMRADSTPISHNLAVTPSFSPIPQQSNKSIGNHKNTSVANVSSKVANSKFERNISMM 518

Query: 162 RTASA 148
             ++A
Sbjct: 519 SNSNA 523


>SPAPJ698.03c |prp12|sap130|U2 snRNP-associated protein Sap130
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1206

 Score = 26.2 bits (55), Expect = 2.8
 Identities = 16/55 (29%), Positives = 27/55 (49%), Gaps = 2/55 (3%)
 Frame = +2

Query: 293 IENGVFDVLVTGDDRVEKVVHTYEGSGSFGELALMY--NMPRAASVRAQTAGALW 451
           ++   F +L TGD  + K+   ++G G+  EL L Y   +P A  +     G L+
Sbjct: 313 MKGSFFYLLQTGDGDLLKLTIEHDGQGNVVELRLKYFDTVPLAVQLNILKTGFLF 367


>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 663

 Score = 26.2 bits (55), Expect = 2.8
 Identities = 20/80 (25%), Positives = 37/80 (46%), Gaps = 4/80 (5%)
 Frame = +2

Query: 107 PAVFPKSDAQRARLAEAVRGILLFRSLDAQQMQQVLDAMFEKRSEPGEYVIRQGDDGDNF 286
           PA F  +  Q  + A  + G+ + R ++      +   + +  +E  ++++     G  F
Sbjct: 178 PAYFNDAQRQATKDAGTIAGLNVIRIVNEPTAAAIAYGLDKTDTE--KHIVVYDLGGGTF 235

Query: 287 YV----IENGVFDVLVTGDD 334
            V    I+NGVF+VL T  D
Sbjct: 236 DVSLLSIDNGVFEVLATSGD 255


>SPBC713.09 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 395

 Score = 25.8 bits (54), Expect = 3.7
 Identities = 21/68 (30%), Positives = 34/68 (50%), Gaps = 3/68 (4%)
 Frame = +2

Query: 134 QRARLAEAVRG--ILLFRSLDAQQMQQVLDAM-FEKRSEPGEYVIRQGDDGDNFYVIENG 304
           ++  L EAV    + +    + Q+  + L+AM FE   +PG    +   D  NF VI+N 
Sbjct: 320 EKQLLEEAVERYKLAMVEFAECQRRLKELEAMHFE---QPGSVTEKLFPDPTNFEVIDNV 376

Query: 305 VFDVLVTG 328
             D+ +TG
Sbjct: 377 SIDLTLTG 384


>SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 332

 Score = 25.8 bits (54), Expect = 3.7
 Identities = 19/63 (30%), Positives = 33/63 (52%), Gaps = 1/63 (1%)
 Frame = -1

Query: 331 VSGDQDVENAVLDDVEVVAVIALSDYVLAGLGSLFEHRIQNL-LHLLRVKRTEQQYAPDR 155
           +  D+D+ + V DDV+ V +I      +A LG      +Q+L L   ++K+ E    P+ 
Sbjct: 26  IDADEDLLDDVPDDVDCVELIQSRIQSMASLGLERFKNLQSLCLRQNQIKKIES--VPET 83

Query: 154 LSE 146
           L+E
Sbjct: 84  LTE 86


>SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 891

 Score = 25.8 bits (54), Expect = 3.7
 Identities = 12/29 (41%), Positives = 17/29 (58%)
 Frame = +3

Query: 78  PKRMILTKEPLPCSPSRTHREPVSLRRSG 164
           P R   +++PLP SPSRT    + +  SG
Sbjct: 78  PVRSPSSRKPLPASPSRTRDHSLRVPVSG 106


>SPAC11H11.04 |mam2||pheromone p-factor receptor|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 348

 Score = 25.4 bits (53), Expect = 4.9
 Identities = 9/34 (26%), Positives = 20/34 (58%)
 Frame = +2

Query: 338 VEKVVHTYEGSGSFGELALMYNMPRAASVRAQTA 439
           ++  +HTY+G  S  +  L+ ++P ++   + TA
Sbjct: 267 IDSFIHTYDGFSSMTQCLLIISLPLSSLWASSTA 300


>SPAC6F12.05c |tnr3||thiamine diphosphokinase Tnr3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 569

 Score = 25.0 bits (52), Expect = 6.5
 Identities = 10/20 (50%), Positives = 13/20 (65%)
 Frame = -1

Query: 256 YVLAGLGSLFEHRIQNLLHL 197
           +VL G+G   +H I NL HL
Sbjct: 450 FVLCGMGGRVDHAIGNLNHL 469


>SPCC584.05 |sec1||SNARE binding protein Sec1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 693

 Score = 25.0 bits (52), Expect = 6.5
 Identities = 22/91 (24%), Positives = 39/91 (42%), Gaps = 2/91 (2%)
 Frame = -1

Query: 313 VENAVLDDVEVVAVIALSDYVLAGLGSLFEHRIQNLLHLLRVKRTEQQYAPDRLSETGSL 134
           ++N +  D E    +     +LAGL    E R Q  LHL   +     +   +L+  G++
Sbjct: 311 IDNTLFLDKERATSLNDMRSMLAGLSDFQELRDQYSLHLTMAQECMNIFEKQQLNLIGAI 370

Query: 133 --CVRLGEHGRGSFVRIILFGVISLGENGFA 47
              +  G +  G   R +L  ++ L + G A
Sbjct: 371 EQDLSTGSNVEGKVPRSVLSELLPLLDEGNA 401


>SPBC1539.10 |||ribosome biogenesis protein Nop16
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 209

 Score = 24.6 bits (51), Expect = 8.6
 Identities = 11/36 (30%), Positives = 21/36 (58%)
 Frame = +2

Query: 188 DAQQMQQVLDAMFEKRSEPGEYVIRQGDDGDNFYVI 295
           D +   + LD + +K   PG+ ++R+ DDG+   +I
Sbjct: 79  DRELTSEELDEL-KKSLPPGQAIVRRDDDGNIIEII 113


>SPAC2G11.06 |vps4||AAA family ATPase Vps4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 432

 Score = 24.6 bits (51), Expect = 8.6
 Identities = 17/57 (29%), Positives = 27/57 (47%), Gaps = 3/57 (5%)
 Frame = -1

Query: 166 APDRLSETGSLCVRLGE---HGRGSFVRIILFGVISLGENGFAPIVKARHWRLFYKI 5
           A + L ET  L ++L +   HGR  +  I+L+G    G++  A  V       F+ I
Sbjct: 137 AKEALKETVLLPIKLPQLFSHGRKPWSGILLYGPPGTGKSYLAKAVATEAGSTFFSI 193


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,117,973
Number of Sequences: 5004
Number of extensions: 44045
Number of successful extensions: 197
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 194
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 202220600
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -