BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30528 (508 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC8C9.03 |cgs1||cAMP-dependent protein kinase regulatory subun... 81 1e-16 SPAC3G6.01 |hrp3||ATP-dependent DNA helicase Hrp3|Schizosaccharo... 27 1.2 SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe... 27 1.6 SPCP1E11.02 |ppk38||Ark1/Prk1 family protein kinase Ppk38|Schizo... 27 2.1 SPAC637.13c |||cytoskeletal signaling protein|Schizosaccharomyce... 27 2.1 SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3 ... 26 2.8 SPAPJ698.03c |prp12|sap130|U2 snRNP-associated protein Sap130 |S... 26 2.8 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 26 2.8 SPBC713.09 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 3.7 SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22... 26 3.7 SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |S... 26 3.7 SPAC11H11.04 |mam2||pheromone p-factor receptor|Schizosaccharomy... 25 4.9 SPAC6F12.05c |tnr3||thiamine diphosphokinase Tnr3 |Schizosacchar... 25 6.5 SPCC584.05 |sec1||SNARE binding protein Sec1|Schizosaccharomyces... 25 6.5 SPBC1539.10 |||ribosome biogenesis protein Nop16 |Schizosaccharo... 25 8.6 SPAC2G11.06 |vps4||AAA family ATPase Vps4|Schizosaccharomyces po... 25 8.6 >SPAC8C9.03 |cgs1||cAMP-dependent protein kinase regulatory subunit Cgs1|Schizosaccharomyces pombe|chr 1|||Manual Length = 412 Score = 80.6 bits (190), Expect = 1e-16 Identities = 57/143 (39%), Positives = 77/143 (53%), Gaps = 12/143 (8%) Frame = +2 Query: 44 RRKSVFAETYDPEEDDSDEGAPAVFPKSDAQRA-RLAEAVRGILLFRSLDAQQMQQVLDA 220 RR+SV E+ +P + FP D + RL +V G LF++LD + +VL+A Sbjct: 101 RRQSVSTESMNPSAFALE--TKRTFPPKDPEDLKRLKRSVAGNFLFKNLDEEHYNEVLNA 158 Query: 221 MFEKR-SEPGEYVIRQGDDGDNFYVIENGVFDVL------VTGDDRVE----KVVHTYEG 367 M EKR E G VI QG GD FY++E G FDV +T ++ + + T Sbjct: 159 MTEKRIGEAGVAVIVQGAVGDYFYIVEQGEFDVYKRPELNITPEEVLSSGYGNYITTISP 218 Query: 368 SGSFGELALMYNMPRAASVRAQT 436 FGELALMYN PRAASV ++T Sbjct: 219 GEYFGELALMYNAPRAASVVSKT 241 Score = 64.1 bits (149), Expect = 1e-11 Identities = 40/108 (37%), Positives = 59/108 (54%) Frame = +2 Query: 113 VFPKSDAQRARLAEAVRGILLFRSLDAQQMQQVLDAMFEKRSEPGEYVIRQGDDGDNFYV 292 VF + QR + + + SLD Q Q++ DA+ + G VIRQGD G+ FY+ Sbjct: 258 VFENAYRQRMLYESLLEEVPILSSLDKYQRQKIADALQTVVYQAGSIVIRQGDIGNQFYL 317 Query: 293 IENGVFDVLVTGDDRVEKVVHTYEGSGSFGELALMYNMPRAASVRAQT 436 IE+G +V+ G + VV T FGELAL++ R A+V+A+T Sbjct: 318 IEDGEAEVVKNG----KGVVVTLTKGDYFGELALIHETVRNATVQAKT 361 >SPAC3G6.01 |hrp3||ATP-dependent DNA helicase Hrp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1388 Score = 27.5 bits (58), Expect = 1.2 Identities = 17/63 (26%), Positives = 31/63 (49%) Frame = +2 Query: 62 AETYDPEEDDSDEGAPAVFPKSDAQRARLAEAVRGILLFRSLDAQQMQQVLDAMFEKRSE 241 AE+ EE +SD+ + PK +++ + V+ + F S +++ + DA FE E Sbjct: 111 AESVTSEESESDDSSYGGTPKKRSRQKKSNTYVQDEIRFSSRNSKGVNYNEDAYFESFEE 170 Query: 242 PGE 250 E Sbjct: 171 EEE 173 >SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 317 Score = 27.1 bits (57), Expect = 1.6 Identities = 24/91 (26%), Positives = 41/91 (45%), Gaps = 3/91 (3%) Frame = -2 Query: 363 SYVWTTFSTRSSPV---TRTSKTPFSMT*KLSPSSPCLITYSPGSDLFSNIASRTCCICC 193 SY T ST +P TRTS + F + +PSS + S S S +S + Sbjct: 122 SYSGTISSTSIAPSMIGTRTSSSYFITSSSSTPSSSSSSSSSSPSSSSSKSSSSSKSSSS 181 Query: 192 ASRERNSSMPRTASARRALCASDLGNTAGAP 100 +S SS ++S++ + +S ++ +P Sbjct: 182 SSSSSKSSSSSSSSSKSSSSSSSSSKSSASP 212 >SPCP1E11.02 |ppk38||Ark1/Prk1 family protein kinase Ppk38|Schizosaccharomyces pombe|chr 3|||Manual Length = 650 Score = 26.6 bits (56), Expect = 2.1 Identities = 15/46 (32%), Positives = 24/46 (52%), Gaps = 2/46 (4%) Frame = -2 Query: 339 TRSSPVTRT--SKTPFSMT*KLSPSSPCLITYSPGSDLFSNIASRT 208 T+ +P + T P K+SP++P L T + D+FS+ A T Sbjct: 416 TQGAPPSHTYGPPPPVQPKPKISPTTPRLSTLALADDMFSSTAKET 461 >SPAC637.13c |||cytoskeletal signaling protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 498 Score = 26.6 bits (56), Expect = 2.1 Identities = 12/49 (24%), Positives = 25/49 (51%) Frame = +2 Query: 74 DPEEDDSDEGAPAVFPKSDAQRARLAEAVRGILLFRSLDAQQMQQVLDA 220 D ++DD ++ PAV +S R+++ + RS ++Q+ D+ Sbjct: 426 DDDDDDPNDFRPAVERQSSTMNTRMSQPSSAVNTNRSYGSEQIPSYADS 474 >SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 932 Score = 26.2 bits (55), Expect = 2.8 Identities = 19/65 (29%), Positives = 32/65 (49%), Gaps = 3/65 (4%) Frame = -2 Query: 333 SSPVTRTSKTPFSMT*KLSPS---SPCLITYSPGSDLFSNIASRTCCICCASRERNSSMP 163 SSP+ R TP S ++PS P S G+ +++A+ + + + ERN SM Sbjct: 459 SSPIMRADSTPISHNLAVTPSFSPIPQQSNKSIGNHKNTSVANVSSKVANSKFERNISMM 518 Query: 162 RTASA 148 ++A Sbjct: 519 SNSNA 523 >SPAPJ698.03c |prp12|sap130|U2 snRNP-associated protein Sap130 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1206 Score = 26.2 bits (55), Expect = 2.8 Identities = 16/55 (29%), Positives = 27/55 (49%), Gaps = 2/55 (3%) Frame = +2 Query: 293 IENGVFDVLVTGDDRVEKVVHTYEGSGSFGELALMY--NMPRAASVRAQTAGALW 451 ++ F +L TGD + K+ ++G G+ EL L Y +P A + G L+ Sbjct: 313 MKGSFFYLLQTGDGDLLKLTIEHDGQGNVVELRLKYFDTVPLAVQLNILKTGFLF 367 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 26.2 bits (55), Expect = 2.8 Identities = 20/80 (25%), Positives = 37/80 (46%), Gaps = 4/80 (5%) Frame = +2 Query: 107 PAVFPKSDAQRARLAEAVRGILLFRSLDAQQMQQVLDAMFEKRSEPGEYVIRQGDDGDNF 286 PA F + Q + A + G+ + R ++ + + + +E ++++ G F Sbjct: 178 PAYFNDAQRQATKDAGTIAGLNVIRIVNEPTAAAIAYGLDKTDTE--KHIVVYDLGGGTF 235 Query: 287 YV----IENGVFDVLVTGDD 334 V I+NGVF+VL T D Sbjct: 236 DVSLLSIDNGVFEVLATSGD 255 >SPBC713.09 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 395 Score = 25.8 bits (54), Expect = 3.7 Identities = 21/68 (30%), Positives = 34/68 (50%), Gaps = 3/68 (4%) Frame = +2 Query: 134 QRARLAEAVRG--ILLFRSLDAQQMQQVLDAM-FEKRSEPGEYVIRQGDDGDNFYVIENG 304 ++ L EAV + + + Q+ + L+AM FE +PG + D NF VI+N Sbjct: 320 EKQLLEEAVERYKLAMVEFAECQRRLKELEAMHFE---QPGSVTEKLFPDPTNFEVIDNV 376 Query: 305 VFDVLVTG 328 D+ +TG Sbjct: 377 SIDLTLTG 384 >SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22 |Schizosaccharomyces pombe|chr 1|||Manual Length = 332 Score = 25.8 bits (54), Expect = 3.7 Identities = 19/63 (30%), Positives = 33/63 (52%), Gaps = 1/63 (1%) Frame = -1 Query: 331 VSGDQDVENAVLDDVEVVAVIALSDYVLAGLGSLFEHRIQNL-LHLLRVKRTEQQYAPDR 155 + D+D+ + V DDV+ V +I +A LG +Q+L L ++K+ E P+ Sbjct: 26 IDADEDLLDDVPDDVDCVELIQSRIQSMASLGLERFKNLQSLCLRQNQIKKIES--VPET 83 Query: 154 LSE 146 L+E Sbjct: 84 LTE 86 >SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 891 Score = 25.8 bits (54), Expect = 3.7 Identities = 12/29 (41%), Positives = 17/29 (58%) Frame = +3 Query: 78 PKRMILTKEPLPCSPSRTHREPVSLRRSG 164 P R +++PLP SPSRT + + SG Sbjct: 78 PVRSPSSRKPLPASPSRTRDHSLRVPVSG 106 >SPAC11H11.04 |mam2||pheromone p-factor receptor|Schizosaccharomyces pombe|chr 1|||Manual Length = 348 Score = 25.4 bits (53), Expect = 4.9 Identities = 9/34 (26%), Positives = 20/34 (58%) Frame = +2 Query: 338 VEKVVHTYEGSGSFGELALMYNMPRAASVRAQTA 439 ++ +HTY+G S + L+ ++P ++ + TA Sbjct: 267 IDSFIHTYDGFSSMTQCLLIISLPLSSLWASSTA 300 >SPAC6F12.05c |tnr3||thiamine diphosphokinase Tnr3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 569 Score = 25.0 bits (52), Expect = 6.5 Identities = 10/20 (50%), Positives = 13/20 (65%) Frame = -1 Query: 256 YVLAGLGSLFEHRIQNLLHL 197 +VL G+G +H I NL HL Sbjct: 450 FVLCGMGGRVDHAIGNLNHL 469 >SPCC584.05 |sec1||SNARE binding protein Sec1|Schizosaccharomyces pombe|chr 3|||Manual Length = 693 Score = 25.0 bits (52), Expect = 6.5 Identities = 22/91 (24%), Positives = 39/91 (42%), Gaps = 2/91 (2%) Frame = -1 Query: 313 VENAVLDDVEVVAVIALSDYVLAGLGSLFEHRIQNLLHLLRVKRTEQQYAPDRLSETGSL 134 ++N + D E + +LAGL E R Q LHL + + +L+ G++ Sbjct: 311 IDNTLFLDKERATSLNDMRSMLAGLSDFQELRDQYSLHLTMAQECMNIFEKQQLNLIGAI 370 Query: 133 --CVRLGEHGRGSFVRIILFGVISLGENGFA 47 + G + G R +L ++ L + G A Sbjct: 371 EQDLSTGSNVEGKVPRSVLSELLPLLDEGNA 401 >SPBC1539.10 |||ribosome biogenesis protein Nop16 |Schizosaccharomyces pombe|chr 2|||Manual Length = 209 Score = 24.6 bits (51), Expect = 8.6 Identities = 11/36 (30%), Positives = 21/36 (58%) Frame = +2 Query: 188 DAQQMQQVLDAMFEKRSEPGEYVIRQGDDGDNFYVI 295 D + + LD + +K PG+ ++R+ DDG+ +I Sbjct: 79 DRELTSEELDEL-KKSLPPGQAIVRRDDDGNIIEII 113 >SPAC2G11.06 |vps4||AAA family ATPase Vps4|Schizosaccharomyces pombe|chr 1|||Manual Length = 432 Score = 24.6 bits (51), Expect = 8.6 Identities = 17/57 (29%), Positives = 27/57 (47%), Gaps = 3/57 (5%) Frame = -1 Query: 166 APDRLSETGSLCVRLGE---HGRGSFVRIILFGVISLGENGFAPIVKARHWRLFYKI 5 A + L ET L ++L + HGR + I+L+G G++ A V F+ I Sbjct: 137 AKEALKETVLLPIKLPQLFSHGRKPWSGILLYGPPGTGKSYLAKAVATEAGSTFFSI 193 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,117,973 Number of Sequences: 5004 Number of extensions: 44045 Number of successful extensions: 197 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 177 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 194 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 202220600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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