Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30525
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g60740.1 68414.m06838 peroxiredoxin type 2, putative strong s... 118 3e-27
At1g65980.1 68414.m07486 peroxiredoxin type 2, putative strong s... 117 4e-27
At1g65970.1 68414.m07485 peroxiredoxin type 2, putative strong s... 117 5e-27
At3g52960.1 68416.m05838 peroxiredoxin type 2, putative similar ... 107 4e-24
At1g65990.1 68414.m07488 type 2 peroxiredoxin-related / thiol sp... 95 2e-20
At3g06050.1 68416.m00692 alkyl hydroperoxide reductase/thiol spe... 85 4e-17
At1g48130.1 68414.m05371 peroxiredoxin (PER1) / rehydrin, putati... 39 0.002
At3g26060.1 68416.m03245 peroxiredoxin Q, putative similar to pe... 33 0.11
At3g02890.1 68416.m00284 PHD finger protein-related contains low... 31 0.61
At5g46250.2 68418.m05694 RNA recognition motif (RRM)-containing ... 29 1.9
At5g46250.1 68418.m05693 RNA recognition motif (RRM)-containing ... 29 1.9
At5g07360.1 68418.m00840 amidase family protein low similarity t... 29 2.5
At1g55320.1 68414.m06319 acyl-activating enzyme 18 (AAE18) nearl... 29 2.5
At1g58440.1 68414.m06648 squalene monooxygenase, putative / squa... 27 7.5
At1g30795.1 68414.m03765 hydroxyproline-rich glycoprotein family... 27 7.5
At4g26400.2 68417.m03800 zinc finger (C3HC4-type RING finger) fa... 27 9.9
At4g26400.1 68417.m03799 zinc finger (C3HC4-type RING finger) fa... 27 9.9
At2g20170.1 68415.m02358 hypothetical protein and grail contain... 27 9.9
At1g28610.2 68414.m03523 GDSL-motif lipase, putative similar to ... 27 9.9
>At1g60740.1 68414.m06838 peroxiredoxin type 2, putative strong
similarity to type 2 peroxiredoxin [Brassica rapa subsp.
pekinensis] GI:4928472; contains Pfam profile: PF00578
AhpC/TSA (alkyl hydroperoxide reductase and
thiol-specific antioxidant) family
Length = 162
Score = 118 bits (283), Expect = 3e-27
Identities = 56/122 (45%), Positives = 77/122 (63%), Gaps = 3/122 (2%)
Frame = +1
Query: 151 MAPIKVGDQLPAADLF---EDSPANKVNICELTAGKKVVLFAVPGAFTPGCSKTHLPGYV 321
MAPI VGD +P + E+ V++ + AGKKV+LF VPGAFTP CS +H+PG++
Sbjct: 1 MAPITVGDVVPDGTISFFDENDQLQTVSVHSIAAGKKVILFGVPGAFTPTCSMSHVPGFI 60
Query: 322 QNADKLKSDGVAEIVCVSVNDPYVMAAWGAQHNTKGKVRMLADPSGNFIKALDLGTNLPP 501
A++LKS G+ EI+C SVNDP+VM AWG + V+ +AD SG + L L +L
Sbjct: 61 GKAEELKSKGIDEIICFSVNDPFVMKAWGKTYQENKHVKFVADGSGEYTHLLGLELDLKD 120
Query: 502 LG 507
G
Sbjct: 121 KG 122
>At1g65980.1 68414.m07486 peroxiredoxin type 2, putative strong
similarity to type 2 peroxiredoxin [Brassica rapa subsp.
pekinensis] GI:4928472; contains Pfam profile: PF00578
AhpC/TSA (alkyl hydroperoxide reductase and
thiol-specific antioxidant) family
Length = 162
Score = 117 bits (282), Expect = 4e-27
Identities = 57/122 (46%), Positives = 76/122 (62%), Gaps = 3/122 (2%)
Frame = +1
Query: 151 MAPIKVGDQLPAADLF---EDSPANKVNICELTAGKKVVLFAVPGAFTPGCSKTHLPGYV 321
MAPI VGD +P + E+ ++ L AGKKV+LF VPGAFTP CS H+PG++
Sbjct: 1 MAPIAVGDVVPDGTISFFDENDQLQTASVHSLAAGKKVILFGVPGAFTPTCSMKHVPGFI 60
Query: 322 QNADKLKSDGVAEIVCVSVNDPYVMAAWGAQHNTKGKVRMLADPSGNFIKALDLGTNLPP 501
+ A++LKS GV EI+C SVNDP+VM AWG + V+ +AD SG + L L +L
Sbjct: 61 EKAEELKSKGVDEIICFSVNDPFVMKAWGKTYPENKHVKFVADGSGEYTHLLGLELDLKD 120
Query: 502 LG 507
G
Sbjct: 121 KG 122
>At1g65970.1 68414.m07485 peroxiredoxin type 2, putative strong
similarity to type 2 peroxiredoxin [Brassica rapa subsp.
pekinensis] GI:4928472; contains Pfam profile: PF00578
AhpC/TSA (alkyl hydroperoxide reductase and
thiol-specific antioxidant) family
Length = 162
Score = 117 bits (281), Expect = 5e-27
Identities = 56/122 (45%), Positives = 77/122 (63%), Gaps = 3/122 (2%)
Frame = +1
Query: 151 MAPIKVGDQLPAADLF---EDSPANKVNICELTAGKKVVLFAVPGAFTPGCSKTHLPGYV 321
MAPI VGD +P + E+ V++ + AGKKV+LF VPGAFTP CS +H+PG++
Sbjct: 1 MAPITVGDVVPDGTISFFDENDQLQTVSVHSIAAGKKVILFGVPGAFTPTCSMSHVPGFI 60
Query: 322 QNADKLKSDGVAEIVCVSVNDPYVMAAWGAQHNTKGKVRMLADPSGNFIKALDLGTNLPP 501
A++LKS G+ EI+C SVNDP+VM AWG + V+ +AD SG + L L +L
Sbjct: 61 GKAEELKSKGIDEIICFSVNDPFVMKAWGKTYPENKHVKFVADGSGEYTHLLGLELDLKD 120
Query: 502 LG 507
G
Sbjct: 121 KG 122
>At3g52960.1 68416.m05838 peroxiredoxin type 2, putative similar to
type 2 peroxiredoxin [Brassica rapa subsp. pekinensis]
GI:4928472; contains Pfam profile: PF00578 AhpC/TSA
(alkyl hydroperoxide reductase and thiol-specific
antioxidant) family
Length = 234
Score = 107 bits (257), Expect = 4e-24
Identities = 58/135 (42%), Positives = 79/135 (58%), Gaps = 5/135 (3%)
Frame = +1
Query: 106 TNRASARALHISQLSM-APIKVGDQLPAADLFEDSPAN----KVNICELTAGKKVVLFAV 270
TN ASA + + A I VGD+LP + L P+ V + LTAGKK +LFAV
Sbjct: 54 TNSASATTRSFATTPVTASISVGDKLPDSTLSYLDPSTGDVKTVTVSSLTAGKKTILFAV 113
Query: 271 PGAFTPGCSKTHLPGYVQNADKLKSDGVAEIVCVSVNDPYVMAAWGAQHNTKGKVRMLAD 450
PGAFTP CS+ H+PG+V A +L+S G+ I C+SVND +VM AW +V +L+D
Sbjct: 114 PGAFTPTCSQKHVPGFVSKAGELRSKGIDVIACISVNDAFVMEAWRKDLGINDEVMLLSD 173
Query: 451 PSGNFIKALDLGTNL 495
+G F L + +L
Sbjct: 174 GNGEFTGKLGVELDL 188
>At1g65990.1 68414.m07488 type 2 peroxiredoxin-related / thiol
specific antioxidant / mal allergen family protein
similar to type 2 peroxiredoxin [Brassica rapa subsp.
pekinensis] GI:4928472; contains Pfam profiles PF00646:
F-box domain, PF00578: AhpC/TSA family
Length = 553
Score = 95.5 bits (227), Expect = 2e-20
Identities = 48/114 (42%), Positives = 72/114 (63%), Gaps = 3/114 (2%)
Frame = +1
Query: 151 MAPIKVGDQLPAADLF---EDSPANKVNICELTAGKKVVLFAVPGAFTPGCSKTHLPGYV 321
MAPI VGD +P + +D V++ L AGKKV+LF VPGAF P CS H+ G++
Sbjct: 1 MAPIDVGDFVPDGSISFFDDDDQLQTVSVHSLAAGKKVILFGVPGAFPPTCSMNHVNGFI 60
Query: 322 QNADKLKSDGVAEIVCVSVNDPYVMAAWGAQHNTKGKVRMLADPSGNFIKALDL 483
+ A++LKS+GV EI+C+S +DP+++ A + V+ + D SG +I+ L L
Sbjct: 61 EKAEELKSNGVDEIICLSGDDPFMITACSENKH----VKFVEDGSGEYIQLLGL 110
>At3g06050.1 68416.m00692 alkyl hydroperoxide reductase/thiol
specific antioxidant (AhpC/TSA)/mal allergen family
protein identical to SP|Q9M7T0 Putative peroxiredoxin,
mitochondrial precursor {Arabidopsis thaliana}; similar
to thioredoxin peroxidase [Capsicum annuum] GI:18654477;
contains Pfam profile: PF00578 AhpC/TSA (alkyl
hydroperoxide reductase and thiol-specific antioxidant)
family
Length = 201
Score = 84.6 bits (200), Expect = 4e-17
Identities = 35/90 (38%), Positives = 54/90 (60%)
Frame = +1
Query: 226 ICELTAGKKVVLFAVPGAFTPGCSKTHLPGYVQNADKLKSDGVAEIVCVSVNDPYVMAAW 405
+ ++ GKKVV+F +PGA+T CS+ H+P Y + DK K+ G+ ++CVSVNDP+ + W
Sbjct: 67 LSDIFKGKKVVIFGLPGAYTGVCSQQHVPSYKSHIDKFKAKGIDSVICVSVNDPFAINGW 126
Query: 406 GAQHNTKGKVRMLADPSGNFIKALDLGTNL 495
+ K + D G F K+L L +L
Sbjct: 127 AEKLGAKDAIEFYGDFDGKFHKSLGLDKDL 156
>At1g48130.1 68414.m05371 peroxiredoxin (PER1) / rehydrin, putative
identical to peroxiredoxin (Rehydrin homolog)
[Arabidopsis thaliana] SWISS-PROT:O04005; contains Pfam
profile: PF00578 AhpC/TSA (alkyl hydroperoxide reductase
and thiol-specific antioxidant) family
Length = 216
Score = 38.7 bits (86), Expect = 0.002
Identities = 31/116 (26%), Positives = 55/116 (47%), Gaps = 5/116 (4%)
Frame = +1
Query: 151 MAPIKVGDQLPAADLFEDSPANKVNICELTAGKKVVLFAVPGAFTPGCSKTHLPGYVQNA 330
M I +GD +P ++ ++ +K + + A VLF+ PG FTP C+ T L + A
Sbjct: 1 MPGITLGDTVPNLEV--ETTHDKFKLHDYFANSWTVLFSHPGDFTPVCT-TELGAMAKYA 57
Query: 331 DKLKSDGVAEIVCVSVNDPYVMAAW-----GAQHNTKGKVRMLADPSGNFIKALDL 483
+ GV +++ +S +D W H +K ++ADP+ I L++
Sbjct: 58 HEFDKRGV-KLLGLSCDDVQSHKDWIKDIEAFNHGSKVNYPIIADPNKEIIPQLNM 112
>At3g26060.1 68416.m03245 peroxiredoxin Q, putative similar to
peroxiredoxin Q [Sedum lineare] GI:6899842; contains
Pfam profile: PF00578 AhpC/TSA (alkyl hydroperoxide
reductase and thiol-specific antioxidant) family
Length = 216
Score = 33.1 bits (72), Expect = 0.11
Identities = 31/109 (28%), Positives = 53/109 (48%)
Frame = +1
Query: 145 LSMAPIKVGDQLPAADLFEDSPANKVNICELTAGKKVVLFAVPGAFTPGCSKTHLPGYVQ 324
L A + G P L +D V++ + GK VVL+ P TPGC+K +
Sbjct: 64 LIFAKVNKGQAAPDFTL-KDQNGKPVSLKKYK-GKPVVLYFYPADETPGCTK-QACAFRD 120
Query: 325 NADKLKSDGVAEIVCVSVNDPYVMAAWGAQHNTKGKVRMLADPSGNFIK 471
+ +K K G AE++ +S +D A+ +++ K +L+D GN ++
Sbjct: 121 SYEKFKKAG-AEVIGISGDDSASHKAFASKY--KLPYTLLSD-EGNKVR 165
>At3g02890.1 68416.m00284 PHD finger protein-related contains low
similarity to PHD-finger domain proteins
Length = 963
Score = 30.7 bits (66), Expect = 0.61
Identities = 21/75 (28%), Positives = 39/75 (52%)
Frame = -2
Query: 476 RALMKLPLGSASIRTFPLVLC*APQAAITYGSLTDTHTISATPSDFSLSAFCTYPGKCVL 297
R +++ G+ ++ + P C A + GS +D S S L++ C++ G +L
Sbjct: 4 RGRLEIQSGTCNVCSAPCSSCMHHNAEFS-GSKSDES--SDENSHGVLASQCSFNGDNLL 60
Query: 296 EHPGVKAPGTANNTT 252
GV APG+++NT+
Sbjct: 61 RSSGVNAPGSSHNTS 75
>At5g46250.2 68418.m05694 RNA recognition motif (RRM)-containing
protein contains similarity to RNA-binding protein;
contains InterPro entry IPR000504: RNA-binding region
RNP-1 (RNA recognition motif) (RRM)
Length = 420
Score = 29.1 bits (62), Expect = 1.9
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = +3
Query: 393 DGGLGSSAQH*RKGAYASRSQRQLHQGSGPGHQSAAARRFP 515
DGG ++ KG + +RQ HQG G GH +A++ P
Sbjct: 338 DGGNHQKDKNGNKGRVVGQGRRQNHQG-GNGHGTASSSSHP 377
>At5g46250.1 68418.m05693 RNA recognition motif (RRM)-containing
protein contains similarity to RNA-binding protein;
contains InterPro entry IPR000504: RNA-binding region
RNP-1 (RNA recognition motif) (RRM)
Length = 422
Score = 29.1 bits (62), Expect = 1.9
Identities = 15/42 (35%), Positives = 23/42 (54%), Gaps = 1/42 (2%)
Frame = +3
Query: 393 DGGLGSSAQH*RKGAYASRSQRQLHQ-GSGPGHQSAAARRFP 515
DGG ++ KG + +RQ HQ G+G GH +A++ P
Sbjct: 338 DGGNHQKDKNGNKGRVVGQGRRQNHQGGNGIGHGTASSSSHP 379
>At5g07360.1 68418.m00840 amidase family protein low similarity to
enantiomerase-selective amidase [Rhodococcus sp.]
GI:152052; contains Pfam profile PF01425: Amidase
Length = 659
Score = 28.7 bits (61), Expect = 2.5
Identities = 20/77 (25%), Positives = 30/77 (38%), Gaps = 2/77 (2%)
Frame = -2
Query: 503 SGGRLVPRSRALMKLPLGS--ASIRTFPLVLC*APQAAITYGSLTDTHTISATPSDFSLS 330
S G S ++ +GS A T+P C T+GS+ T +S + S L
Sbjct: 349 SAGPAASTSAGMVPFAIGSETAGSMTYPAARCGITALRPTFGSVGRTGVMSISESLDKLG 408
Query: 329 AFCTYPGKCVLEHPGVK 279
FC C + +K
Sbjct: 409 PFCRTAADCAVILDAIK 425
>At1g55320.1 68414.m06319 acyl-activating enzyme 18 (AAE18) nearly
identical to acyl-activating enzyme 18 [Arabidopsis
thaliana] GI:29893268; similar to acetyl-CoA synthetase
[SP|P27095] from Methanothrix soehngenii; contains Pfam
AMP-binding enzyme domain PF00501l; identical to cDNA
acyl-activating enzyme 18 (At1g55320) GI: 29893267
Length = 725
Score = 28.7 bits (61), Expect = 2.5
Identities = 13/32 (40%), Positives = 19/32 (59%)
Frame = +1
Query: 196 FEDSPANKVNICELTAGKKVVLFAVPGAFTPG 291
F+DSP N++ I EL +V A+ G+F G
Sbjct: 199 FDDSPVNRMTIKELREQVMLVANAISGSFEKG 230
>At1g58440.1 68414.m06648 squalene monooxygenase, putative /
squalene epoxidase, putative similar to SP|O65404 (SE
1,1), SP|O65402 (SE 1,2) 6566341 dbj AB008021.1 AB008021
Length = 531
Score = 27.1 bits (57), Expect = 7.5
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = -2
Query: 368 HTISATPSDFSLSAFCTYPGKCVLEHPGVKAPGTAN 261
H + A PS + + +C+++ PG K P AN
Sbjct: 257 HVVLADPSPILMYPISSTEVRCLVDVPGQKVPSIAN 292
>At1g30795.1 68414.m03765 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR000694
Length = 109
Score = 27.1 bits (57), Expect = 7.5
Identities = 14/43 (32%), Positives = 19/43 (44%)
Frame = -3
Query: 274 PAPQIIQPSFPPSTHKYSPY*RENLRINQQQAADPRP*LVPCL 146
P P ++P FPP +P+ I Q P P +PCL
Sbjct: 54 PLPPFLRPFFPPLQFAAAPF---GGNIPQPPLPSPPPTFLPCL 93
>At4g26400.2 68417.m03800 zinc finger (C3HC4-type RING finger)
family protein contains Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 356
Score = 26.6 bits (56), Expect = 9.9
Identities = 13/27 (48%), Positives = 17/27 (62%), Gaps = 1/27 (3%)
Frame = +3
Query: 57 SNVSHRVFDYPWHYR-IHQSSVSASTS 134
SNVS R F +PW + + SS S+S S
Sbjct: 319 SNVSERRFSFPWPFSGLFSSSSSSSAS 345
>At4g26400.1 68417.m03799 zinc finger (C3HC4-type RING finger)
family protein contains Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 356
Score = 26.6 bits (56), Expect = 9.9
Identities = 13/27 (48%), Positives = 17/27 (62%), Gaps = 1/27 (3%)
Frame = +3
Query: 57 SNVSHRVFDYPWHYR-IHQSSVSASTS 134
SNVS R F +PW + + SS S+S S
Sbjct: 319 SNVSERRFSFPWPFSGLFSSSSSSSAS 345
>At2g20170.1 68415.m02358 hypothetical protein and grail contains
Pfam profile PF03080: Arabidopsis proteins of unknown
function
Length = 401
Score = 26.6 bits (56), Expect = 9.9
Identities = 20/65 (30%), Positives = 28/65 (43%), Gaps = 2/65 (3%)
Frame = +1
Query: 289 GCSKTHLPGYVQNADKLKSDGVAEIVCVSVNDPYVMAAWGAQHNTKGKVRMLA--DPSGN 462
GC T PG+VQ + KL +A + Y + A Q N G L +P G
Sbjct: 232 GCYNTVCPGFVQVSSKLALGTLARPTSTYDGEQYYLQAIIYQDNITGNWWFLIKNEPIGY 291
Query: 463 FIKAL 477
+ K+L
Sbjct: 292 WPKSL 296
>At1g28610.2 68414.m03523 GDSL-motif lipase, putative similar to
lipase [Arabidopsis thaliana] GI:1145627; contains Pfam
profile: PF00657 Lipase/Acylhydrolase with GDSL-like
motif
Length = 383
Score = 26.6 bits (56), Expect = 9.9
Identities = 14/29 (48%), Positives = 18/29 (62%)
Frame = +1
Query: 262 FAVPGAFTPGCSKTHLPGYVQNADKLKSD 348
F VPG F GCS T+L Y Q ++K + D
Sbjct: 215 FLVPGNFPLGCSATYLTLY-QTSNKEEYD 242
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,305,486
Number of Sequences: 28952
Number of extensions: 242453
Number of successful extensions: 669
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 617
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 666
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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