Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30523
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g02210.1 68416.m00202 phytochelatin synthetase family protein... 29 1.9
At4g12440.1 68417.m01968 adenine phosphoribosyltransferase, puta... 29 2.5
At3g43540.1 68416.m04622 expressed protein hypothetical protein ... 29 2.5
At3g29810.1 68416.m03794 phytochelatin synthetase family protein... 29 2.5
At2g33420.1 68415.m04096 expressed protein 28 4.3
At2g41540.2 68415.m05134 NAD-dependent glycerol-3-phosphate dehy... 27 5.7
At2g41540.1 68415.m05133 NAD-dependent glycerol-3-phosphate dehy... 27 5.7
At5g60920.1 68418.m07642 phytochelatin synthetase, putative / CO... 27 7.5
At3g46610.1 68416.m05060 pentatricopeptide (PPR) repeat-containi... 27 7.5
At3g18860.2 68416.m02396 transducin family protein / WD-40 repea... 27 7.5
At3g18860.1 68416.m02395 transducin family protein / WD-40 repea... 27 7.5
At3g12380.1 68416.m01543 actin/actin-like family protein similar... 27 9.9
At1g48880.1 68414.m05476 hypothetical protein 27 9.9
>At3g02210.1 68416.m00202 phytochelatin synthetase family protein /
COBRA cell expansion protein COBL3 similar to
phytochelatin synthetase [Hordeum vulgare subsp.
vulgare] GI:29570314; identified in Roudier, et al,
Plant Phys. (2002) 130:538-548 (PMID:12376623);
supported by cDNA: gi|26452134|dbj|AK118555.1; contains
Pfam profile PF04833: Phytochelatin synthetase-like
conserved region
Length = 452
Score = 29.1 bits (62), Expect = 1.9
Identities = 15/50 (30%), Positives = 29/50 (58%)
Frame = -3
Query: 484 EMSIENFRSYYSSLQYWHPVVRYPASSIITLLETFNHSFSSPYPLIDFSG 335
+++I NF +Y + W+ VV++P +T +FN+ +PY I+ +G
Sbjct: 312 KVTITNF-NYNMNYSQWNLVVQHPNFDNLTQTFSFNYKPLTPYASINDTG 360
>At4g12440.1 68417.m01968 adenine phosphoribosyltransferase,
putative strong similarity to SP|P31166 Adenine
phosphoribosyltransferase 1 (EC 2.4.2.7) (APRT)
{Arabidopsis thaliana}; contains Pfam profile PF00156:
Phosphoribosyl transferase domain
Length = 178
Score = 28.7 bits (61), Expect = 2.5
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = -1
Query: 114 CKREDAIELLYSFFGSINLWGKILTEFQWVTI 19
C + + +SFF +I+L K+L F+W+ I
Sbjct: 139 CAAMNLLSKFFSFFNNISLVPKLLLSFKWLPI 170
>At3g43540.1 68416.m04622 expressed protein hypothetical protein
slr1699 - Synechocystis sp. (strain PCC 6803),
PIR:S75306
Length = 373
Score = 28.7 bits (61), Expect = 2.5
Identities = 27/94 (28%), Positives = 41/94 (43%), Gaps = 1/94 (1%)
Frame = +2
Query: 65 MLPKKL*SNSMASSRLQVTARQLSKTFKKKGRFEIGIASELPPAYKKFWREWKVLKPAAV 244
++P L S + L VTA S+ K R + + PP +KK K L A V
Sbjct: 15 LIPATLFSKRARALSLTVTATGGSRNNKVYTRLDSCLVIPPPPKFKKPRAIVKFLGGAFV 74
Query: 245 HFVPQ-EGKWKRDELTGETYPIQNIPIPLKFPAE 343
VP+ + ++ L E Y I ++P + F E
Sbjct: 75 GAVPELTYSYLKELLAKEGYLIVSVPYNVTFDHE 108
>At3g29810.1 68416.m03794 phytochelatin synthetase family protein /
COBRA cell expansion protein COBL2 similar to
phytochelatin synthetase [Hordeum vulgare subsp.
vulgare] GI:29570314; identified in Roudier, et al,
Plant Phys. (2002) 130:538-548 (PMID:12376623); contains
Pfam profile PF04833: Phytochelatin synthetase-like
conserved region
Length = 441
Score = 28.7 bits (61), Expect = 2.5
Identities = 14/43 (32%), Positives = 26/43 (60%)
Frame = -3
Query: 484 EMSIENFRSYYSSLQYWHPVVRYPASSIITLLETFNHSFSSPY 356
+++I NF +Y + W+ VV++P +T L +FN+ +PY
Sbjct: 303 KVAITNF-NYNMNYSQWNLVVQHPNFDNLTKLFSFNYKPLNPY 344
>At2g33420.1 68415.m04096 expressed protein
Length = 1039
Score = 27.9 bits (59), Expect = 4.3
Identities = 13/27 (48%), Positives = 20/27 (74%)
Frame = +3
Query: 294 KLIRYKIFLFLLNSPLKSMRGYGEEKL 374
KL R +++ LLNS L SM+G+ E++L
Sbjct: 405 KLDREALYVKLLNSTLASMQGWTEKRL 431
>At2g41540.2 68415.m05134 NAD-dependent glycerol-3-phosphate
dehydrogenase family protein weak similarity to
SP|P46919 Glycerol-3-phosphate dehydrogenase [NAD(P)+]
(EC 1.1.1.94) {Bacillus subtilis}; contains Pfam profile
PF01210: NAD-dependent glycerol-3-phosphate
dehydrogenase
Length = 462
Score = 27.5 bits (58), Expect = 5.7
Identities = 11/38 (28%), Positives = 16/38 (42%), Gaps = 2/38 (5%)
Frame = +2
Query: 323 PLKFPAEINEGIWGGEAVVKGFQKRDDRR--RRVPHYW 430
PLK + E +W + VV G + R + YW
Sbjct: 145 PLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYW 182
>At2g41540.1 68415.m05133 NAD-dependent glycerol-3-phosphate
dehydrogenase family protein weak similarity to
SP|P46919 Glycerol-3-phosphate dehydrogenase [NAD(P)+]
(EC 1.1.1.94) {Bacillus subtilis}; contains Pfam profile
PF01210: NAD-dependent glycerol-3-phosphate
dehydrogenase
Length = 462
Score = 27.5 bits (58), Expect = 5.7
Identities = 11/38 (28%), Positives = 16/38 (42%), Gaps = 2/38 (5%)
Frame = +2
Query: 323 PLKFPAEINEGIWGGEAVVKGFQKRDDRR--RRVPHYW 430
PLK + E +W + VV G + R + YW
Sbjct: 145 PLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYW 182
>At5g60920.1 68418.m07642 phytochelatin synthetase, putative / COBRA
cell expansion protein COB, putative similar to
phytochelatin synthetase GI:29570314; similar to
GB:AAK56072; identified in Roudier, et al, Plant Phys.
(2002) 130:538-548 (PMID:12376623); identical to cDNA
putative phytochelatin synthetase GI:3559804; contains
Pfam profile PF04833: Phytochelatin synthetase-like
conserved region
Length = 456
Score = 27.1 bits (57), Expect = 7.5
Identities = 13/43 (30%), Positives = 25/43 (58%)
Frame = -3
Query: 484 EMSIENFRSYYSSLQYWHPVVRYPASSIITLLETFNHSFSSPY 356
+++I NF +Y + W+ V ++P IT + +FN+ +PY
Sbjct: 317 KITITNF-NYRLNYTQWNLVAQHPNLDNITQIFSFNYKSLTPY 358
>At3g46610.1 68416.m05060 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 665
Score = 27.1 bits (57), Expect = 7.5
Identities = 21/68 (30%), Positives = 33/68 (48%)
Frame = +3
Query: 180 QSFHLLIKNFGVSGRY*SQQLYISFLKKGSGNATN*LVKLIRYKIFLFLLNSPLKSMRGY 359
Q F +IK FG R + +LK+ + +I +F++ NS L +MRG+
Sbjct: 147 QVFCAMIKGFGKDKRLKPAVAVVDWLKRKKSESGG----VIGPNLFIY--NSLLGAMRGF 200
Query: 360 GEEKLWLK 383
GE + LK
Sbjct: 201 GEAEKILK 208
>At3g18860.2 68416.m02396 transducin family protein / WD-40 repeat
family protein contains seven G-protein beta WD-40
repeats; similar to phospholipase a-2-activating protein
SP:P27612 from [Mus musculus]
Length = 760
Score = 27.1 bits (57), Expect = 7.5
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = -3
Query: 445 LQYWHPVVRYPASSIITLLETFNHSFS 365
LQ W P + +PA+ I+ +L +H S
Sbjct: 564 LQAWPPAMMFPATDIVRMLVLHHHGAS 590
>At3g18860.1 68416.m02395 transducin family protein / WD-40 repeat
family protein contains seven G-protein beta WD-40
repeats; similar to phospholipase a-2-activating protein
SP:P27612 from [Mus musculus]
Length = 760
Score = 27.1 bits (57), Expect = 7.5
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = -3
Query: 445 LQYWHPVVRYPASSIITLLETFNHSFS 365
LQ W P + +PA+ I+ +L +H S
Sbjct: 564 LQAWPPAMMFPATDIVRMLVLHHHGAS 590
>At3g12380.1 68416.m01543 actin/actin-like family protein similar to
SP|P53946 Actin-like protein ARP5 {Saccharomyces
cerevisiae}; contains Pfam profile PF00022: Actin
Length = 724
Score = 26.6 bits (56), Expect = 9.9
Identities = 10/33 (30%), Positives = 17/33 (51%)
Frame = +2
Query: 254 PQEGKWKRDELTGETYPIQNIPIPLKFPAEINE 352
P E + D L E YP+ N+P + P ++ +
Sbjct: 371 PAEYEENPDSLNNEKYPLMNVPDDILTPEQLKD 403
>At1g48880.1 68414.m05476 hypothetical protein
Length = 485
Score = 26.6 bits (56), Expect = 9.9
Identities = 16/61 (26%), Positives = 31/61 (50%), Gaps = 3/61 (4%)
Frame = -3
Query: 502 IRYSY*EMSIENFRSYY---SSLQYWHPVVRYPASSIITLLETFNHSFSSPYPLIDFSGE 332
+R+S ++E +RS + WH R ++ + +L+ NH +SS LI +G+
Sbjct: 230 VRFSSYNFTVEFYRSVFLVQPGRLRWHAPKRVKSTLKLDVLDVINHEWSSADFLIFNTGQ 289
Query: 331 F 329
+
Sbjct: 290 W 290
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,180,589
Number of Sequences: 28952
Number of extensions: 237519
Number of successful extensions: 701
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 691
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 701
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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