Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30516
         (516 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    88   8e-19
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce...    50   2e-07
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    48   8e-07
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    45   6e-06
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    39   4e-04
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi...    33   0.025
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos...    31   0.10 
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha...    27   1.7  
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch...    26   3.8  
SPAC11D3.05 |||membrane transporter|Schizosaccharomyces pombe|ch...    25   5.1  
SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma...    25   5.1  
SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosacc...    25   6.7  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 87.8 bits (208), Expect = 8e-19
 Identities = 43/108 (39%), Positives = 70/108 (64%)
 Frame = +3

Query: 144 LPPEQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRL 323
           L  EQIA  R+AF  FD ++ G+I  + +  ++R +GQ      L+++I EVDAD +G +
Sbjct: 6   LTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTI 65

Query: 324 EFPEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREI 467
           +F EF+T+ A+ + + D E   +E+REAF+++DK+GNGYI    L  +
Sbjct: 66  DFTEFLTMMARKMKDTDNE---EEVREAFKVFDKDGNGYITVEELTHV 110


>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 174

 Score = 50.0 bits (114), Expect = 2e-07
 Identities = 33/102 (32%), Positives = 51/102 (50%)
 Frame = +3

Query: 153 EQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFP 332
           E+I  +RK F   D N+SGSI  +    I  +   P    +   L   VD D  G ++F 
Sbjct: 21  EEIERIRKRFIKIDANQSGSIDRNEFLSIPSVASNP----LASRLFSVVDEDGGGDVDFQ 76

Query: 333 EFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSL 458
           EF+   + F V  + E   ++L+ AF++YD + +GYI    L
Sbjct: 77  EFINSLSVFSVHGNKE---EKLKFAFKIYDIDRDGYISNGEL 115


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 48.0 bits (109), Expect = 8e-07
 Identities = 29/109 (26%), Positives = 54/109 (49%)
 Frame = +3

Query: 141 ELPPEQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGR 320
           E+  EQ   + +AF  FD ++  +I    +   +R +G    K  + +++ + D    G 
Sbjct: 30  EITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGY 89

Query: 321 LEFPEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREI 467
           L+  +FV +  + IVE D     +E++ AF L+D +  G I   +LR +
Sbjct: 90  LQMEDFVRVMTEKIVERDP---LEEIKRAFELFDDDETGKISLRNLRRV 135



 Score = 39.1 bits (87), Expect = 4e-04
 Identities = 21/75 (28%), Positives = 40/75 (53%)
 Frame = +3

Query: 123 TEDWADELPPEQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVD 302
           TE   +  P E+I   ++AF+ FD + +G I    +  + + + +  + + LE +IEE D
Sbjct: 100 TEKIVERDPLEEI---KRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFD 156

Query: 303 ADKSGRLEFPEFVTL 347
            D+ G +   EF+ +
Sbjct: 157 LDQDGEINEQEFIAI 171


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 45.2 bits (102), Expect = 6e-06
 Identities = 28/104 (26%), Positives = 55/104 (52%)
 Frame = +3

Query: 153 EQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFP 332
           EQ   +++AF  +D ++ G IP   V  +LR +G       L +L  E+       ++  
Sbjct: 6   EQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL----GDAIDEK 61

Query: 333 EFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLRE 464
           +F++  +  + E ++E   +E  +AFR++DK+ +GYI T+   +
Sbjct: 62  KFMSFVSNKLRETESE---EEYIKAFRVFDKDNSGYIETAKFAD 102



 Score = 29.1 bits (62), Expect = 0.41
 Identities = 12/25 (48%), Positives = 18/25 (72%)
 Frame = +3

Query: 393 ELREAFRLYDKEGNGYIPTSSLREI 467
           E++EAF LYD + +G IPTS +  +
Sbjct: 10  EMKEAFVLYDIDKDGLIPTSHVGSV 34


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 39.1 bits (87), Expect = 4e-04
 Identities = 26/99 (26%), Positives = 49/99 (49%)
 Frame = +3

Query: 171 RKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFPEFVTLA 350
           ++AF  FD + +G IP   + D+LR  GQ  N  + E  I E+++     ++  +F+ + 
Sbjct: 9   KQAFSLFDRHGTGRIPKTSIGDLLRACGQ--NPTLAE--ITEIESTLPAEVDMEQFLQVL 64

Query: 351 AKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREI 467
            +     D     +E  + F+++DK+  G I    LR +
Sbjct: 65  NR-PNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYV 102


>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
           Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 190

 Score = 33.1 bits (72), Expect = 0.025
 Identities = 21/63 (33%), Positives = 32/63 (50%)
 Frame = +3

Query: 279 EELIEEVDADKSGRLEFPEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSL 458
           E +    DADK+G ++F EF+   +   V    E +  +L  AF+LYD + NG I    +
Sbjct: 66  EYVFNVFDADKNGYIDFKEFICALS---VTSRGE-LNDKLIWAFQLYDLDNNGLISYDEM 121

Query: 459 REI 467
             I
Sbjct: 122 LRI 124


>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
           Plc1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 899

 Score = 31.1 bits (67), Expect = 0.10
 Identities = 15/53 (28%), Positives = 28/53 (52%)
 Frame = +3

Query: 180 FDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFPEF 338
           ++  +  +S  +    V  + +M+    + + LEE  ++ DAD SG+L F EF
Sbjct: 303 WEKLEKEQSAQLDLGDVHRMCQMLHLNASMEFLEETFQKADADHSGKLSFEEF 355


>SPAC926.03 |rlc1||myosin II regulatory light chain
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 184

 Score = 27.1 bits (57), Expect = 1.7
 Identities = 11/18 (61%), Positives = 15/18 (83%)
 Frame = +3

Query: 390 KELREAFRLYDKEGNGYI 443
           +EL+EAF L DK+G+G I
Sbjct: 48  QELKEAFALLDKDGDGNI 65


>SPAC1687.14c |||EF hand family protein, unknown
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 76

 Score = 25.8 bits (54), Expect = 3.8
 Identities = 12/26 (46%), Positives = 14/26 (53%)
 Frame = +3

Query: 384 MQKELREAFRLYDKEGNGYIPTSSLR 461
           M +E  EAF L+D    GYI    LR
Sbjct: 10  MDEEAEEAFDLFDVTHKGYIDFEDLR 35


>SPAC11D3.05 |||membrane transporter|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 546

 Score = 25.4 bits (53), Expect = 5.1
 Identities = 9/18 (50%), Positives = 11/18 (61%)
 Frame = +3

Query: 33  NPERWKHSELWPVVLCVS 86
           NP  W HS  W +V+ VS
Sbjct: 96  NPRNWSHSYKWWIVIQVS 113


>SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 614

 Score = 25.4 bits (53), Expect = 5.1
 Identities = 11/22 (50%), Positives = 15/22 (68%)
 Frame = +3

Query: 276 LEELIEEVDADKSGRLEFPEFV 341
           + E I EV+ D SGR+E  +FV
Sbjct: 56  VREAIREVNVDSSGRVEPEDFV 77


>SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 830

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 15/34 (44%), Positives = 20/34 (58%)
 Frame = +3

Query: 330 PEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEG 431
           PEF   AA+ +VEED E +     EA  + D+EG
Sbjct: 38  PEFSDEAAEALVEEDIEDLD---GEALDVEDEEG 68


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,908,856
Number of Sequences: 5004
Number of extensions: 34464
Number of successful extensions: 145
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 138
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)