BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30510 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 78 7e-16 SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 78 9e-16 SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 52 4e-08 SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 41 1e-04 SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 40 2e-04 SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 27 1.7 SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|c... 25 6.7 >SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 78.2 bits (184), Expect = 7e-16 Identities = 43/106 (40%), Positives = 63/106 (59%), Gaps = 6/106 (5%) Frame = +2 Query: 215 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 382 + +Q+AEF+EAF L D D+DG I N+L SLG+ + EL +M+ E +G I+ Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66 Query: 383 FTQLLTLFANRMSGGSDEDDVVINAFKTFDEEGK--IDSERLRHAL 514 FT+ LT+ A +M +D ++ V AFK FD++G I E L H L Sbjct: 67 FTEFLTMMARKMK-DTDNEEEVREAFKVFDKDGNGYITVEELTHVL 111 Score = 37.1 bits (82), Expect = 0.002 Identities = 20/65 (30%), Positives = 36/65 (55%), Gaps = 4/65 (6%) Frame = +2 Query: 233 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLLT 400 E +EAF++ D D +G I +L SLG S++E+ +M+ EA G IN+ + Sbjct: 86 EVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSR 145 Query: 401 LFANR 415 + +++ Sbjct: 146 VISSK 150 >SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 184 Score = 77.8 bits (183), Expect = 9e-16 Identities = 43/117 (36%), Positives = 63/117 (53%), Gaps = 2/117 (1%) Frame = +2 Query: 170 SRKAKRTGSNVFSMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELD 349 ++ AKR S F+ + Q+ E KEAF L+D D DG IG+ D++ SL + ASE ++ Sbjct: 28 AQAAKRASSGAFAQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSIN 87 Query: 350 EMVGEASGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFD--EEGKIDSERLRHAL 514 M + PIN LT + + S +D ++ AF TFD + GKI +R AL Sbjct: 88 HMFESINPPINLAAFLTAMGSMLCRISPRND-LLEAFSTFDDTQSGKIPISTMRDAL 143 >SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces pombe|chr 1|||Manual Length = 143 Score = 52.4 bits (120), Expect = 4e-08 Identities = 28/88 (31%), Positives = 50/88 (56%) Frame = +2 Query: 215 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQL 394 S++Q E KEAF L D DKDG+I + + + SLG ++ EL ++ E I+ + Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63 Query: 395 LTLFANRMSGGSDEDDVVINAFKTFDEE 478 ++ +N++ E++ I AF+ FD++ Sbjct: 64 MSFVSNKLRETESEEE-YIKAFRVFDKD 90 >SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pombe|chr 1|||Manual Length = 141 Score = 40.7 bits (91), Expect = 1e-04 Identities = 18/42 (42%), Positives = 26/42 (61%) Frame = +2 Query: 233 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 358 EF + FQ+ D D G+IG +LR SLG S +E+DE++ Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELL 119 >SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 39.9 bits (89), Expect = 2e-04 Identities = 29/103 (28%), Positives = 51/103 (49%), Gaps = 6/103 (5%) Frame = +2 Query: 215 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 382 +++Q + EAF+L D DKD I ++LRA +LG A + E+ +++ + G + Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQ 91 Query: 383 FTQLLTLFANRMSGGSDEDDVVINAFKTF--DEEGKIDSERLR 505 + + ++ D + + AF+ F DE GKI LR Sbjct: 92 MEDFVRVMTEKIV-ERDPLEEIKRAFELFDDDETGKISLRNLR 133 >SPAC1687.14c |||EF hand family protein, unknown role|Schizosaccharomyces pombe|chr 1|||Manual Length = 76 Score = 27.1 bits (57), Expect = 1.7 Identities = 15/42 (35%), Positives = 22/42 (52%) Frame = +2 Query: 233 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 358 E +EAF L D G I DLR + LG ++++L M+ Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLML 54 >SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1274 Score = 25.0 bits (52), Expect = 6.7 Identities = 12/38 (31%), Positives = 21/38 (55%) Frame = +2 Query: 224 QVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASE 337 + A+FK+ F DKD + + +L A + L +LA + Sbjct: 212 EYAKFKDKFPEAPLDKDNLQTQKELEARIECLKQLAEK 249 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,532,602 Number of Sequences: 5004 Number of extensions: 22717 Number of successful extensions: 97 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 90 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 96 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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