BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30508 (516 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g13950.1 68414.m01639 eukaryotic translation initiation facto... 201 3e-52 At1g69410.1 68414.m07972 eukaryotic translation initiation facto... 194 3e-50 At1g26630.1 68414.m03243 eukaryotic translation initiation facto... 190 6e-49 At1g56345.1 68414.m06477 pseudouridine synthase family protein l... 29 1.4 At5g14230.1 68418.m01663 ankyrin repeat family protein contains ... 29 1.9 At5g41460.1 68418.m05035 fringe-related protein strong similarit... 29 2.5 At5g03140.1 68418.m00262 lectin protein kinase family protein co... 29 2.5 At3g05830.1 68416.m00654 expressed protein 29 2.5 At3g12240.1 68416.m01527 serine carboxypeptidase S10 family prot... 28 3.2 At3g53330.1 68416.m05884 plastocyanin-like domain-containing pro... 28 4.3 At3g06490.1 68416.m00753 myb family transcription factor (MYB108... 28 4.3 At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein... 28 4.3 At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-... 27 5.7 At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative simila... 27 5.7 At1g20860.1 68414.m02613 phosphate transporter family protein si... 27 5.7 At3g58930.1 68416.m06567 F-box family protein contains F-box dom... 27 7.5 At3g51290.1 68416.m05614 proline-rich family protein 27 7.5 At2g27680.1 68415.m03354 aldo/keto reductase family protein cont... 27 7.5 At5g64280.1 68418.m08075 oxoglutarate/malate translocator, putat... 27 9.9 At4g12460.1 68417.m01971 oxysterol-binding family protein simila... 27 9.9 At4g11430.1 68417.m01841 hydroxyproline-rich glycoprotein family... 27 9.9 At3g09770.2 68416.m01158 zinc finger (C3HC4-type RING finger) fa... 27 9.9 At3g09770.1 68416.m01157 zinc finger (C3HC4-type RING finger) fa... 27 9.9 At1g34355.1 68414.m04265 forkhead-associated domain-containing p... 27 9.9 At1g12380.1 68414.m01431 expressed protein 27 9.9 >At1g13950.1 68414.m01639 eukaryotic translation initiation factor 5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Arabidopsis thaliana} Length = 158 Score = 201 bits (490), Expect = 3e-52 Identities = 87/150 (58%), Positives = 117/150 (78%), Gaps = 1/150 (0%) Frame = +2 Query: 68 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 247 E+ HFE+ D+GAS T+P Q +RKNG++++K RPCK+VE+STSKTGKHGHAK H V ID Sbjct: 4 EEHHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63 Query: 248 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGD-LG 424 IF KK EDI PS+HN DVPHV R DYQL DIS+DGY++L+ DNG ++DLK+P+ D L Sbjct: 64 IFTSKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLL 123 Query: 425 TQLRTDFDSGKELLCTVLKSCGEECVIAVK 514 Q+++ FD GK+L+ +V+ + GEE + A+K Sbjct: 124 QQIKSGFDDGKDLVVSVMSAMGEEQINALK 153 >At1g69410.1 68414.m07972 eukaryotic translation initiation factor 5A, putative / eIF-5A, putative strong similarity to eukaryotic initiation factor 5A (2) (Nicotiana plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Lycopersicon esculentum} Length = 158 Score = 194 bits (473), Expect = 3e-50 Identities = 85/150 (56%), Positives = 115/150 (76%), Gaps = 1/150 (0%) Frame = +2 Query: 68 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 247 ++ HFE+ D+GAS T+P Q +RK G +++KGRPCK+VE+STSKTGKHGHAK H V ID Sbjct: 4 DEHHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAID 63 Query: 248 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLG 424 IF KK EDI PS+HN DVPHV R DYQL DIS+DG+++L+ DNG ++DLK+P D L Sbjct: 64 IFTSKKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALL 123 Query: 425 TQLRTDFDSGKELLCTVLKSCGEECVIAVK 514 TQL+ F+ GK+++ +V+ + GEE + A+K Sbjct: 124 TQLKNGFEEGKDIVVSVMSAMGEEQMCALK 153 >At1g26630.1 68414.m03243 eukaryotic translation initiation factor 5A, putative / eIF-5A, putative strong similariy to SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Lycopersicon esculentum} Length = 159 Score = 190 bits (462), Expect = 6e-49 Identities = 83/150 (55%), Positives = 111/150 (74%), Gaps = 1/150 (0%) Frame = +2 Query: 68 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 247 ++ HFE +SGAS T+P +RK G +++K RPCK+VE+STSKTGKHGHAK H V ID Sbjct: 4 DEHHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63 Query: 248 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLG 424 IF KK EDI PS+HN DVPHV R DYQL DI++DG+++L+ D+G ++DLK+P D L Sbjct: 64 IFTAKKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLT 123 Query: 425 TQLRTDFDSGKELLCTVLKSCGEECVIAVK 514 Q+R FD GK+++ +V+ S GEE + AVK Sbjct: 124 AQMRLGFDEGKDIVVSVMSSMGEEQICAVK 153 >At1g56345.1 68414.m06477 pseudouridine synthase family protein low similarity to SP|P23851 Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate synthase) (Uracil hydrolyase) {Escherichia coli}; contains Pfam profile PF00849: RNA pseudouridylate synthase Length = 322 Score = 29.5 bits (63), Expect = 1.4 Identities = 20/69 (28%), Positives = 34/69 (49%), Gaps = 1/69 (1%) Frame = -3 Query: 478 QHGAQQFLAAVEVSTQLCAEVTIRDFEVLTQVTIV-SHQGQVAIIRDISQLVVFALHVGY 302 +HGA + AA++V L +RD E +V V S + + + D++ ++V G Sbjct: 176 KHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSVNSVKNESCELEDVNHVIVAE---GE 232 Query: 301 VHVVCGGTD 275 + CGG D Sbjct: 233 RELSCGGDD 241 >At5g14230.1 68418.m01663 ankyrin repeat family protein contains ankyrin repeats, Pfam:PF00023 Length = 591 Score = 29.1 bits (62), Expect = 1.9 Identities = 15/34 (44%), Positives = 16/34 (47%) Frame = +2 Query: 398 LKIPDGDLGTQLRTDFDSGKELLCTVLKSCGEEC 499 L IPDGD T L G +C L SCG C Sbjct: 412 LDIPDGDGYTPLMLAAREGHGHMCEYLISCGANC 445 >At5g41460.1 68418.m05035 fringe-related protein strong similarity to unknown protein (pir||T13026) similarity to predicted proteins + similar to hypothetical protein GB:AAC23643 [Arabidopsis thaliana] + weak similarity to Fringe [Schistocerca gregaria](GI:6573138);Fringe encodes an extracellular protein that regulates Notch signalling. Length = 524 Score = 28.7 bits (61), Expect = 2.5 Identities = 11/29 (37%), Positives = 16/29 (55%) Frame = +3 Query: 33 VVKFKTQQWVTSKTHTSRPETPGPQPPSP 119 ++ F T Q K++ S P +P P PP P Sbjct: 80 LILFHTNQTAVIKSYASPPPSPPPPPPPP 108 >At5g03140.1 68418.m00262 lectin protein kinase family protein contains Pfam domains, PF00138: Legume lectins alpha domain, PF00139: Legume lectins beta domain and PF00069: Protein kinase domain Length = 711 Score = 28.7 bits (61), Expect = 2.5 Identities = 13/36 (36%), Positives = 21/36 (58%) Frame = -1 Query: 168 RPFSITKPFLRRAEHCMGKVAEAPESPVSKCVSSMS 61 +P +T+P +R + A+ PE P++K SSMS Sbjct: 631 QPDPVTRPTMRSVVQILVGEADVPEVPIAKPSSSMS 666 >At3g05830.1 68416.m00654 expressed protein Length = 336 Score = 28.7 bits (61), Expect = 2.5 Identities = 24/82 (29%), Positives = 39/82 (47%) Frame = +2 Query: 221 AKVHLVGIDIFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDL 400 AK + G+D K ED+ P V+ +D ++T + D+ + LM +G + Sbjct: 186 AKAGIGGMDSELQKLLEDVSPMKFERMNRLVEVKDEEITKLKDE--IRLM--SGQWKHKT 241 Query: 401 KIPDGDLGTQLRTDFDSGKELL 466 K + L Q RTD D K++L Sbjct: 242 KELESQLEKQRRTDQDLKKKVL 263 >At3g12240.1 68416.m01527 serine carboxypeptidase S10 family protein contains Pfam profile: PF00450 serine carboxypeptidase; similar to serine carboxypeptidase I precursor (SP:P37890) [Oryza sativa] Length = 436 Score = 28.3 bits (60), Expect = 3.2 Identities = 20/76 (26%), Positives = 33/76 (43%), Gaps = 4/76 (5%) Frame = -3 Query: 385 VTIVSHQGQVAIIRDISQL---VVFALHVGYVHVVCGGTD-IFILFTIEDINPNQVNFSV 218 + ++ H +IIR + + F L GY+ V D +F F + NP + V Sbjct: 16 LVLIQHADSSSIIRYLPGFEGPLPFELETGYIGVGQKEEDQLFYYFIKSENNPEEDPLLV 75 Query: 217 AVLSGFGCGHFNNLAW 170 + G GC F+ L + Sbjct: 76 WLTGGPGCSSFSGLVY 91 >At3g53330.1 68416.m05884 plastocyanin-like domain-containing protein similar to mavicyanin SP:P80728 from [Cucurbita pepo] Length = 310 Score = 27.9 bits (59), Expect = 4.3 Identities = 13/18 (72%), Positives = 13/18 (72%), Gaps = 1/18 (5%) Frame = +3 Query: 66 SKTHT-SRPETPGPQPPS 116 SKTH SRP TP P PPS Sbjct: 127 SKTHERSRPITPSPPPPS 144 Score = 26.6 bits (56), Expect = 9.9 Identities = 12/17 (70%), Positives = 12/17 (70%), Gaps = 1/17 (5%) Frame = +3 Query: 66 SKTHT-SRPETPGPQPP 113 SKTH SRP TP P PP Sbjct: 144 SKTHEPSRPNTPPPPPP 160 >At3g06490.1 68416.m00753 myb family transcription factor (MYB108) identical to transcription factor MYB108 GI:15375290 from [Arabidopsis thaliana] Length = 323 Score = 27.9 bits (59), Expect = 4.3 Identities = 17/56 (30%), Positives = 27/56 (48%), Gaps = 2/56 (3%) Frame = +3 Query: 171 HARLLKCPHPKPESTATLKFTWLGLISSMVKSMKIS--VPPHTTWTYPT*SAKTTN 332 HA+ LKC + T+K+ W+ + ++S S TT T T SA T++ Sbjct: 122 HAKQLKCDVNSQQFKDTMKYLWMPRLVERIQSASASSAAAATTTTTTTTGSAGTSS 177 >At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein contains INTERPRO domain, IPR001878: Zn-finger CCHC type Length = 353 Score = 27.9 bits (59), Expect = 4.3 Identities = 15/45 (33%), Positives = 17/45 (37%), Gaps = 3/45 (6%) Frame = +3 Query: 69 KTHTSRPETPGPQPPS---PCNVRPCVKTVSLC*RVVHARLLKCP 194 KT T P PP PCN PC S + + R CP Sbjct: 22 KTTTKPTAAAAPSPPDIHCPCNAGPCNTVTSKTEKNPNRRFYTCP 66 >At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-box RNA helicase [Chlamydomonas reinhardtii] GI:12044832; contains Pfam profiles PF04408: Helicase associated domain (HA2), PF00271: Helicase conserved C-terminal domain Length = 1226 Score = 27.5 bits (58), Expect = 5.7 Identities = 15/51 (29%), Positives = 28/51 (54%), Gaps = 1/51 (1%) Frame = +2 Query: 311 VKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDL-GTQLRTDFDSGKE 460 V+R+ +++ Y L ADN + + G + GT+++T+FDS +E Sbjct: 350 VRRDGSKMSLAQSKKYSQLNADNAQWEDRQLLRSGAVRGTEVQTEFDSEEE 400 >At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative similar to SP|P35207 Antiviral protein SKI2 {Saccharomyces cerevisiae}; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 1347 Score = 27.5 bits (58), Expect = 5.7 Identities = 17/51 (33%), Positives = 30/51 (58%), Gaps = 6/51 (11%) Frame = +2 Query: 341 ISDDGYLTLMADNG--DLREDLKIPDGDLGTQLR----TDFDSGKELLCTV 475 +SD+ L + A G D+ +++ D DL Q++ + +SG+EL+CTV Sbjct: 1141 MSDEALLQMPAFQGRIDVLKNIGCIDDDLVVQIKGRVACEMNSGEELICTV 1191 >At1g20860.1 68414.m02613 phosphate transporter family protein similar to phosphate transporter [Catharanthus roseus] GI:2208908, inorganic phosphate transporter 1 [Solanum tuberosum] GI:1420871; contains Pfam profile PF00083: major facilitator superfamily protein Length = 534 Score = 27.5 bits (58), Expect = 5.7 Identities = 17/45 (37%), Positives = 20/45 (44%) Frame = +3 Query: 45 KTQQWVTSKTHTSRPETPGPQPPSPCNVRPCVKTVSLC*RVVHAR 179 K Q V S++H S T P PP P P K S C +H R Sbjct: 243 KDMQRVMSRSHISDEATTDPPPPPP---PPSYKLFSRCFFRLHGR 284 >At3g58930.1 68416.m06567 F-box family protein contains F-box domain Pfam:PF00646 Length = 482 Score = 27.1 bits (57), Expect = 7.5 Identities = 26/88 (29%), Positives = 39/88 (44%), Gaps = 11/88 (12%) Frame = -1 Query: 288 VEGQISSYFLPLK----ISIPTK*TL-AWPCFPVLDVDISTILH-----GRPFSITKPFL 139 V G I S FLP K S+ +K L W P+LD+D S LH I + F+ Sbjct: 11 VRGHILS-FLPAKHIALTSVLSKSWLNLWKLIPILDIDDSEFLHPEEGKAERLEIRQSFV 69 Query: 138 RRAEHCMGKVAEAPESPVS-KCVSSMSP 58 + + ++P S KC++ + P Sbjct: 70 DFVDRVLALQDDSPIDRFSLKCITGIHP 97 >At3g51290.1 68416.m05614 proline-rich family protein Length = 602 Score = 27.1 bits (57), Expect = 7.5 Identities = 10/25 (40%), Positives = 13/25 (52%) Frame = +3 Query: 45 KTQQWVTSKTHTSRPETPGPQPPSP 119 +T W T+ T + P P P PP P Sbjct: 91 ETTTWTTTTTSSVLPPPPPPPPPPP 115 >At2g27680.1 68415.m03354 aldo/keto reductase family protein contains Pfam profile PF00248: oxidoreductase, aldo/keto reductase family Length = 384 Score = 27.1 bits (57), Expect = 7.5 Identities = 15/46 (32%), Positives = 24/46 (52%) Frame = +2 Query: 269 EDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKI 406 ++I S MDV + + D ++DGYL + DL+E+ KI Sbjct: 147 QNIDISRKRMDVAALDMLQFHWWDYANDGYLDALKHLTDLKEEGKI 192 >At5g64280.1 68418.m08075 oxoglutarate/malate translocator, putative similar to SWISS-PROT:Q41364 2-oxoglutarate/malate translocator, chloroplast precursor [Spinach]{Spinacia oleracea} Length = 549 Score = 26.6 bits (56), Expect = 9.9 Identities = 15/75 (20%), Positives = 30/75 (40%) Frame = +3 Query: 81 SRPETPGPQPPSPCNVRPCVKTVSLC*RVVHARLLKCPHPKPESTATLKFTWLGLISSMV 260 ++P+ P P PP P K + L V +++ P+PE + + L + + Sbjct: 65 NKPQPPPPSPPQPQG----AKLIPLAISVSIGLIVRFLIPRPEQVTSQGWQLLSIFLFTI 120 Query: 261 KSMKISVPPHTTWTY 305 + + P W + Sbjct: 121 SGLVLGPLPVGAWAF 135 >At4g12460.1 68417.m01971 oxysterol-binding family protein similar to SP|P22059 Oxysterol-binding protein 1 {Homo sapiens}; contains Pfam profiles PF00169: PH domain, PF01237: Oxysterol-binding protein Length = 694 Score = 26.6 bits (56), Expect = 9.9 Identities = 12/36 (33%), Positives = 18/36 (50%) Frame = +2 Query: 71 DTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCK 178 D +E G SG S + +A +G+ +GR CK Sbjct: 367 DQAYEYGKSGKSLLRALNVAAFAVSGYASTEGRHCK 402 >At4g11430.1 68417.m01841 hydroxyproline-rich glycoprotein family protein contains proline-rich extensin domains, INTERPRO:IPR002965; related to hydroxyproline-rich glycoprotein [Phaseolus vulgaris] gi|169349|gb|AAA33765 Length = 219 Score = 26.6 bits (56), Expect = 9.9 Identities = 16/50 (32%), Positives = 19/50 (38%) Frame = +3 Query: 75 HTSRPETPGPQPPSPCNVRPCVKTVSLC*RVVHARLLKCPHPKPESTATL 224 H P P P PP P + P V T + H R P P P T+ Sbjct: 116 HRRSPPPPPPPPPPPPTITPPV-TTTTAGHHHHRRSPPPPPPPPPPPPTI 164 >At3g09770.2 68416.m01158 zinc finger (C3HC4-type RING finger) family protein contains Pfam domain, PF00097: Zinc finger, C3HC4 type (RING finger) Length = 341 Score = 26.6 bits (56), Expect = 9.9 Identities = 15/46 (32%), Positives = 21/46 (45%) Frame = +3 Query: 69 KTHTSRPETPGPQPPSPCNVRPCVKTVSLC*RVVHARLLKCPHPKP 206 + HT+ P P P PPS P + T +V A + P+P P Sbjct: 17 RNHTAAPPPP-PPPPSSSLPPPPLPTEIQANPIVFAAVTPYPNPNP 61 >At3g09770.1 68416.m01157 zinc finger (C3HC4-type RING finger) family protein contains Pfam domain, PF00097: Zinc finger, C3HC4 type (RING finger) Length = 388 Score = 26.6 bits (56), Expect = 9.9 Identities = 15/46 (32%), Positives = 21/46 (45%) Frame = +3 Query: 69 KTHTSRPETPGPQPPSPCNVRPCVKTVSLC*RVVHARLLKCPHPKP 206 + HT+ P P P PPS P + T +V A + P+P P Sbjct: 17 RNHTAAPPPP-PPPPSSSLPPPPLPTEIQANPIVFAAVTPYPNPNP 61 >At1g34355.1 68414.m04265 forkhead-associated domain-containing protein / FHA domain-containing protein Length = 1477 Score = 26.6 bits (56), Expect = 9.9 Identities = 9/28 (32%), Positives = 15/28 (53%) Frame = +2 Query: 41 IQNTTMGDIEDTHFETGDSGASATFPMQ 124 + NT +GD ED H + G ++ P + Sbjct: 341 LANTALGDDEDLHLDVTSEGTGSSVPSE 368 >At1g12380.1 68414.m01431 expressed protein Length = 793 Score = 26.6 bits (56), Expect = 9.9 Identities = 9/19 (47%), Positives = 12/19 (63%) Frame = +3 Query: 63 TSKTHTSRPETPGPQPPSP 119 T+ HT+ +TP P PP P Sbjct: 3 TTNAHTATQQTPPPPPPPP 21 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,153,870 Number of Sequences: 28952 Number of extensions: 272619 Number of successful extensions: 937 Number of sequences better than 10.0: 25 Number of HSP's better than 10.0 without gapping: 850 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 922 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 937669760 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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