BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30494 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 33 0.025 SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex su... 28 0.72 SPBC354.02c |sec61||translocon alpha subunit Sec61|Schizosacchar... 27 2.2 SPCC895.06 |||RNA polymerase II elongator complex subunit Elp2 |... 27 2.2 SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ... 26 3.8 SPBC354.05c |sre2||membrane-tethered transcription factor |Schiz... 25 5.1 SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces pomb... 25 6.7 SPAC30C2.07 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 6.7 SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 25 8.9 >SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 354 Score = 33.1 bits (72), Expect = 0.025 Identities = 15/45 (33%), Positives = 28/45 (62%) Frame = +1 Query: 382 NESEDAKEQRRRNHAINQQLKQDRELYQATHRLLLLGAGESGKST 516 N ++ E ++ N I +Q++ + + +++LLLGA +SGKST Sbjct: 5 NGLSESGESKKLNSKIEKQIENASKKDKKIYKVLLLGASDSGKST 49 >SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex subunit Mtr4|Schizosaccharomyces pombe|chr 1|||Manual Length = 1117 Score = 28.3 bits (60), Expect = 0.72 Identities = 14/27 (51%), Positives = 18/27 (66%) Frame = +1 Query: 418 NHAINQQLKQDRELYQATHRLLLLGAG 498 N+A+NQ ++DREL Q H L LL G Sbjct: 493 NNAVNQLSEKDRELPQIEHILPLLRRG 519 >SPBC354.02c |sec61||translocon alpha subunit Sec61|Schizosaccharomyces pombe|chr 2|||Manual Length = 479 Score = 26.6 bits (56), Expect = 2.2 Identities = 9/21 (42%), Positives = 14/21 (66%) Frame = +1 Query: 427 INQQLKQDRELYQATHRLLLL 489 +N +LK DRE+YQ + L + Sbjct: 103 VNMELKSDREMYQLVQKFLAI 123 >SPCC895.06 |||RNA polymerase II elongator complex subunit Elp2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 760 Score = 26.6 bits (56), Expect = 2.2 Identities = 12/19 (63%), Positives = 15/19 (78%) Frame = +1 Query: 28 PTFDTMYL*SLVISGRDIS 84 PTF+TM L SL+I+G D S Sbjct: 733 PTFETMGLYSLLIAGEDTS 751 >SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 407 Score = 25.8 bits (54), Expect = 3.8 Identities = 10/14 (71%), Positives = 14/14 (100%) Frame = +1 Query: 475 RLLLLGAGESGKST 516 ++LLLGAG+SGK+T Sbjct: 76 KVLLLGAGDSGKTT 89 >SPBC354.05c |sre2||membrane-tethered transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 793 Score = 25.4 bits (53), Expect = 5.1 Identities = 13/33 (39%), Positives = 21/33 (63%) Frame = -3 Query: 223 VLLRRHISYATVVSSAHEFIANLATKYPQLKSE 125 V L R ++ AT++S A E+I +L +K +L E Sbjct: 473 VPLSRKLNKATILSKATEYIKSLQSKNKKLIEE 505 >SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces pombe|chr 1|||Manual Length = 695 Score = 25.0 bits (52), Expect = 6.7 Identities = 19/58 (32%), Positives = 29/58 (50%), Gaps = 7/58 (12%) Frame = +1 Query: 364 SLGGHRNESEDAKEQRRRNHAINQQ--LKQDR-----ELYQATHRLLLLGAGESGKST 516 S+ + S+ K Q+ N + N L QD+ +L + +LLLLG +SGK T Sbjct: 238 SVKSMKKVSQQLKPQKNTNDSNNDHTLLSQDQLIELSKLVKPRTKLLLLGPPKSGKKT 295 >SPAC30C2.07 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 25.0 bits (52), Expect = 6.7 Identities = 15/49 (30%), Positives = 25/49 (51%) Frame = -3 Query: 256 PSPTRPWYCAPVLLRRHISYATVVSSAHEFIANLATKYPQLKSEASSLP 110 P+P Y L + + ++ TVV+S+HE IA+ + + S S P Sbjct: 104 PNPATRSYMLTQLFQIN-THGTVVNSSHETIASATSLFENSSSNFSEDP 151 >SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1436 Score = 24.6 bits (51), Expect = 8.9 Identities = 11/25 (44%), Positives = 16/25 (64%) Frame = -1 Query: 465 LVQLSILFELLIYGMVPSSLLLRVF 391 L Q S LFE L YG + L+++V+ Sbjct: 1200 LCQGSSLFEFLRYGRIEDELVIQVY 1224 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,969,751 Number of Sequences: 5004 Number of extensions: 35551 Number of successful extensions: 89 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 88 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 89 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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