Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30490
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative / (R... 40 0.001
At5g51930.1 68418.m06442 glucose-methanol-choline (GMC) oxidored... 36 0.016
At1g14185.1 68414.m01678 glucose-methanol-choline (GMC) oxidored... 35 0.028
At1g14190.1 68414.m01679 glucose-methanol-choline (GMC) oxidored... 35 0.037
At3g21320.1 68416.m02693 hypothetical protein 30 1.1
At2g05120.1 68415.m00539 expressed protein 29 1.4
At1g12570.1 68414.m01459 glucose-methanol-choline (GMC) oxidored... 29 1.4
At5g51950.1 68418.m06447 glucose-methanol-choline (GMC) oxidored... 29 1.9
At5g18720.1 68418.m02221 hypothetical protein predicted proteins... 29 1.9
At5g23720.1 68418.m02780 dual specificity protein phosphatase fa... 29 2.5
At3g23670.1 68416.m02976 phragmoplast-associated kinesin-related... 27 7.5
At3g02065.2 68416.m00170 DEAD/DEAH box helicase family protein c... 27 7.5
At3g02065.1 68416.m00171 DEAD/DEAH box helicase family protein c... 27 7.5
At2g18950.1 68415.m02212 homogentisate phytylprenyltransferase f... 27 7.5
At5g27270.1 68418.m03254 pentatricopeptide (PPR) repeat-containi... 27 9.9
At5g05100.1 68418.m00541 expressed protein 27 9.9
>At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative /
(R)-oxynitrilase, putative similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|52707];
contains Pfam protile PF00732 GMC oxidoreductase
Length = 552
Score = 39.5 bits (88), Expect = 0.001
Identities = 35/115 (30%), Positives = 52/115 (45%)
Frame = +1
Query: 97 ADATDKVLEDPNYDFIXXXXXXXXXXXXNRLSEISDWKVLLVEAGGNPTLATEIPQPYYS 276
++ATD ED YD+I LS+ ++VLL+E GG P + +
Sbjct: 43 SNATDFASED-YYDYIIVGGGTAGCPLAATLSQ--SFRVLLLERGGVPYNRPNV-MSHDG 98
Query: 277 NMGTSEDWAYHTEPQEGACRAYKNKGCAWPRGKVLGGSSSINLMFYVRGNKADYD 441
+ T D P + +G RG+VLGGSS+IN FY R +K ++
Sbjct: 99 FLTTLTDVNNFDSPAQSFI---SEEGVPNARGRVLGGSSAINAGFYSRADKQFFE 150
>At5g51930.1 68418.m06442 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 582
Score = 35.9 bits (79), Expect = 0.016
Identities = 31/92 (33%), Positives = 40/92 (43%)
Frame = +1
Query: 211 VLLVEAGGNPTLATEIPQPYYSNMGTSEDWAYHTEPQEGACRAYKNKGCAWPRGKVLGGS 390
VL++E GG+P P ++MG S + + P + G R +VLGG
Sbjct: 97 VLVLERGGSP-----YENPTATDMGNSVNTLLNNTPNSWSQLFISEDGVYNTRPRVLGGG 151
Query: 391 SSINLMFYVRGNKADYDEWAADGNEGWSFEEV 486
S IN FY R DY E A W EEV
Sbjct: 152 SVINGGFYSRAGN-DYVEEAE-----WEMEEV 177
>At1g14185.1 68414.m01678 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 503
Score = 35.1 bits (77), Expect = 0.028
Identities = 41/142 (28%), Positives = 62/142 (43%), Gaps = 2/142 (1%)
Frame = +1
Query: 94 PADATD-KVLEDPNYDFIXXXXXXXXXXXXNRLSEISDWKVLLVEAGGNPTLATEIPQPY 270
P TD K + ++D+I LSE + VL++E GG+P +
Sbjct: 23 PYMTTDPKEVSGKSFDYIVVGGGTAGCSLAATLSE--KYSVLVIERGGSPFGDPLVEDKK 80
Query: 271 YSNMGTSEDWAYHTEPQEGACRAYKN-KGCAWPRGKVLGGSSSINLMFYVRGNKADYDEW 447
Y G S +T+ +++ + G RG+VLGGSS+IN FY R + DE+
Sbjct: 81 Y--YGYS---LINTDEYSSVAQSFTSVDGIKNHRGRVLGGSSAINGGFYSRAS----DEF 131
Query: 448 AADGNEGWSFEEVLPYFKKSES 513
GW + V +K ES
Sbjct: 132 VK--KAGWDKDLVQESYKWVES 151
>At1g14190.1 68414.m01679 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 501
Score = 34.7 bits (76), Expect = 0.037
Identities = 38/139 (27%), Positives = 62/139 (44%), Gaps = 7/139 (5%)
Frame = +1
Query: 94 PADATD-KVLEDPNYDFIXXXXXXXXXXXXNRLSEISDWKVLLVEAGGNPTLATEIPQPY 270
P TD K + ++D+I LSE + VL++E GG+P + +
Sbjct: 2 PYMTTDAKEVSGKSFDYIVVGGGTAGCSLAATLSE--KYSVLVIERGGSPFGDPLVEERK 59
Query: 271 YSNMGTSEDWAYHTEPQEGACRAYKN-KGCAWPRGKVLGGSSSINLMFYVRGN-----KA 432
Y G S +T+ +++ + G RG+VLGGSS+IN FY R + KA
Sbjct: 60 Y--FGYS---LLNTDEYSSVAQSFTSVDGIENYRGRVLGGSSAINGGFYSRASDEFVKKA 114
Query: 433 DYDEWAADGNEGWSFEEVL 489
+D+ + W +V+
Sbjct: 115 GWDKGLVQESYKWVESKVV 133
>At3g21320.1 68416.m02693 hypothetical protein
Length = 540
Score = 29.9 bits (64), Expect = 1.1
Identities = 17/54 (31%), Positives = 21/54 (38%)
Frame = -1
Query: 513 AFTFLKVGQDFLETPAFVSIGSPFVVIGLVTPNVKHQIDAAASAEHLTTWPSAS 352
AF GQD LETP + SPF PN + +D W + S
Sbjct: 390 AFMVPVYGQDSLETPFRFPVSSPFSHSYFPPPNARTTVDQTNPFGQFQRWSNTS 443
>At2g05120.1 68415.m00539 expressed protein
Length = 1234
Score = 29.5 bits (63), Expect = 1.4
Identities = 15/35 (42%), Positives = 18/35 (51%), Gaps = 4/35 (11%)
Frame = +1
Query: 424 NKADYDEW----AADGNEGWSFEEVLPYFKKSESF 516
N AD D+W A NEGWS EE L + + F
Sbjct: 1122 NAADQDDWDRHHQASTNEGWSEEETLQNLRNTALF 1156
>At1g12570.1 68414.m01459 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 572
Score = 29.5 bits (63), Expect = 1.4
Identities = 28/100 (28%), Positives = 40/100 (40%), Gaps = 3/100 (3%)
Frame = +1
Query: 211 VLLVEAGGNPTLATEIPQPYYSNMGTSEDWAYHTEPQEGACRAYKNKGCAWPRGKVLGGS 390
VLL+E G +P I + S G + + P + R G R +VLGG
Sbjct: 70 VLLLERGDSPYNNPNITR--LSAFGAALSDLSESSPSQ---RFVSEDGVINARARVLGGG 124
Query: 391 SSINLMFYVRGNKADYDEWAADG---NEGWSFEEVLPYFK 501
S++N FY R DG NE + + E F+
Sbjct: 125 SALNAGFYTRAGTKYVRNMGWDGALANESYQWVEAKVAFQ 164
>At5g51950.1 68418.m06447 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 586
Score = 29.1 bits (62), Expect = 1.9
Identities = 29/88 (32%), Positives = 38/88 (43%), Gaps = 6/88 (6%)
Frame = +1
Query: 211 VLLVEAGG----NPTLATEIPQPYYSNMGTSEDWAYHTEPQEGACRAYKNKGCAWPRGKV 378
VL++E GG NPT AT+I N T+ +T P+ + G R +V
Sbjct: 70 VLVLERGGAPYDNPT-ATDI-----ENFATTLS---NTSPKSWSQLFISEDGVYNTRARV 120
Query: 379 LGGSSSINLMFYVRGNK--ADYDEWAAD 456
LGG S +N FY R EW D
Sbjct: 121 LGGGSVLNAGFYTRAGDEYVKETEWKTD 148
>At5g18720.1 68418.m02221 hypothetical protein predicted proteins -
Arabidopsis thaliana
Length = 552
Score = 29.1 bits (62), Expect = 1.9
Identities = 16/62 (25%), Positives = 30/62 (48%)
Frame = +2
Query: 230 VVILHWQPRYRNLTTVIWGHQKTGLTILNHRKGLVVPTKTRDALGHVVRCSAEAAASI*C 409
++I + +P + + W + +++ N R G TK R HV+ C+ + AS C
Sbjct: 126 LMITYLEPDSNDEKELQWFEKDLPVSVGNFRLGENKSTKDRSIFSHVIHCNERSNAS--C 183
Query: 410 FT 415
F+
Sbjct: 184 FS 185
>At5g23720.1 68418.m02780 dual specificity protein phosphatase
family protein contains Pfam profile: PF00782 dual
specificity phosphatase, catalytic domain; contains TG
acceptor splice site at intron 9
Length = 929
Score = 28.7 bits (61), Expect = 2.5
Identities = 13/33 (39%), Positives = 17/33 (51%)
Frame = +2
Query: 56 WLRNVRSPAITYGQPMLPTRFSKIQTMTSSSSE 154
WL VR + TYG P R +I M +S+ E
Sbjct: 59 WLDLVRKRSATYGSSGFPHRLHRIDDMVTSAGE 91
>At3g23670.1 68416.m02976 phragmoplast-associated kinesin-related
protein, putative similar to kinesin like protein
GB:CAB10194 from [Arabidopsis thaliana]
Length = 1313
Score = 27.1 bits (57), Expect = 7.5
Identities = 14/28 (50%), Positives = 18/28 (64%), Gaps = 1/28 (3%)
Frame = +1
Query: 430 ADYDE-WAADGNEGWSFEEVLPYFKKSE 510
A YDE A+DG+ W EE P++KK E
Sbjct: 1268 AKYDEPSASDGDNQWR-EEFQPFYKKDE 1294
>At3g02065.2 68416.m00170 DEAD/DEAH box helicase family protein
contains Pfam profile: PF00270 DEAD/DEAH box helicase
Length = 505
Score = 27.1 bits (57), Expect = 7.5
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = +3
Query: 357 RLATW*GARRKQQHQFDVLRS 419
+LA W A++K+Q FD+LRS
Sbjct: 332 QLAIWVDAKQKKQKLFDILRS 352
>At3g02065.1 68416.m00171 DEAD/DEAH box helicase family protein
contains Pfam profile: PF00270 DEAD/DEAH box helicase
Length = 368
Score = 27.1 bits (57), Expect = 7.5
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = +3
Query: 357 RLATW*GARRKQQHQFDVLRS 419
+LA W A++K+Q FD+LRS
Sbjct: 195 QLAIWVDAKQKKQKLFDILRS 215
>At2g18950.1 68415.m02212 homogentisate phytylprenyltransferase
family protein (HPT1) / tocopherol phytyltransferase
family protein (TPT1) identical to gi:17104828; contains
Pfam profile PF01040: UbiA prenyltransferase family;
identical to cDNA tocopherol polyprenyltransferase
(TPT1) GI:17104827
Length = 393
Score = 27.1 bits (57), Expect = 7.5
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = -1
Query: 357 ASLVFVGTTSPFLWFSMVSPV 295
A + VG TSPF+W ++S V
Sbjct: 327 AVAILVGATSPFIWSKVISVV 347
>At5g27270.1 68418.m03254 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 1038
Score = 26.6 bits (56), Expect = 9.9
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = +2
Query: 98 PMLPTRFSKIQTMTSSSSELVPPD 169
P +P+R S+I +SSSS V PD
Sbjct: 19 PKIPSRNSRISIKSSSSSSKVRPD 42
>At5g05100.1 68418.m00541 expressed protein
Length = 324
Score = 26.6 bits (56), Expect = 9.9
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = -1
Query: 417 NVKHQIDAAASAEHLTTWPSASLVFVGT 334
N H ++ A +A T WPSA++ + T
Sbjct: 283 NYGHPLNPAMTAPSYTEWPSATMTYAQT 310
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.315 0.133 0.426
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,166,775
Number of Sequences: 28952
Number of extensions: 231295
Number of successful extensions: 662
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 632
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 661
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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