Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30487
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to vil... 166 9e-42
At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to vil... 162 1e-40
At5g57320.1 68418.m07160 villin, putative similar to villin 2 (V... 161 3e-40
At4g30160.1 68417.m04289 villin, putative similar to villin 2 (... 157 5e-39
At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to vil... 94 6e-20
At1g09390.1 68414.m01050 GDSL-motif lipase/hydrolase family prot... 29 1.9
At1g04470.1 68414.m00438 expressed protein EST gb|ATTS5672 comes... 29 1.9
At2g33420.1 68415.m04096 expressed protein 29 2.5
At5g45610.1 68418.m05605 expressed protein 28 3.2
At1g68750.1 68414.m07859 phosphoenolpyruvate carboxylase family ... 28 3.2
At1g56670.1 68414.m06517 GDSL-motif lipase/hydrolase family prot... 28 3.2
At5g10520.1 68418.m01218 protein kinase family protein contains ... 28 4.3
At5g29613.1 68418.m03639 hypothetical protein 27 5.7
At3g23270.1 68416.m02933 regulator of chromosome condensation (R... 27 5.7
At5g49430.1 68418.m06116 transducin family protein / WD-40 repea... 27 7.5
At1g13770.1 68414.m01616 expressed protein contains Pfam domain,... 27 7.5
At3g53320.1 68416.m05883 expressed protein 27 9.9
At3g02750.1 68416.m00267 protein phosphatase 2C family protein /... 27 9.9
>At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to villin
2 (VLN2) [Arabidopsis thaliana] GI:3415115
Length = 976
Score = 166 bits (403), Expect = 9e-42
Identities = 87/147 (59%), Positives = 102/147 (69%), Gaps = 1/147 (0%)
Frame = +1
Query: 67 AFADAGRKPGLEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDK-DRLTWDIHFWLG 243
AF AG+KPG EIWRIENFE V VPK++ G FY GD+YIVL TT +K +DIHFW+G
Sbjct: 9 AFQGAGQKPGTEIWRIENFEAVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWIG 68
Query: 244 SRTSQDEAGAAAILTVNLDDEQFQGSAVQHREVQYYESKEFLEYFSPAIRYLKGGHASGF 423
TSQDEAG AA+ TV L D G AVQHRE+Q +ES +FL YF P I L+GG ASGF
Sbjct: 69 KDTSQDEAGTAAVKTVEL-DAVLGGRAVQHREIQGHESDKFLSYFKPCIIPLEGGVASGF 127
Query: 424 SHVTINEGTEKRLFQIKGKRNVRVKQV 504
V E E RL+ KGKR +R+KQV
Sbjct: 128 KTVE-EEVFETRLYTCKGKRAIRLKQV 153
Score = 57.2 bits (132), Expect = 6e-09
Identities = 37/122 (30%), Positives = 59/122 (48%), Gaps = 2/122 (1%)
Frame = +1
Query: 97 LEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDKDRL-TWDIHFWLGSRTSQDEAGA 273
LE+W + +PK G YSGD Y+VL T +R + + W G ++ ++
Sbjct: 392 LEVWYVNGKVKTPLPKEDIGKLYSGDCYLVLYTYHSGERKDEYFLSCWFGKKSIPEDQD- 450
Query: 274 AAILTVNLDDEQFQGSAVQHREVQYYESKEFLEYFSPAIRYLKGGHASGF-SHVTINEGT 450
AI N +G VQ R + E +F+ F P + LKGG +SG+ S + +E T
Sbjct: 451 TAIRLANTMSNSLKGRPVQGRIYEGKEPPQFVALFQPMV-VLKGGLSSGYKSSMGESEST 509
Query: 451 EK 456
++
Sbjct: 510 DE 511
>At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to villin
3 (VLN3) [Arabidopsis thaliana] GI:3415117
Length = 965
Score = 162 bits (394), Expect = 1e-40
Identities = 85/147 (57%), Positives = 100/147 (68%), Gaps = 1/147 (0%)
Frame = +1
Query: 67 AFADAGRKPGLEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDK-DRLTWDIHFWLG 243
AF G+KPG EIWRIENFEPV VPK++ G FY GD+YIVL TT +K +DIHFW+G
Sbjct: 11 AFQGVGQKPGTEIWRIENFEPVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWIG 70
Query: 244 SRTSQDEAGAAAILTVNLDDEQFQGSAVQHREVQYYESKEFLEYFSPAIRYLKGGHASGF 423
TSQDEAG AA+ TV L D G AVQ+RE+Q +ES +FL YF P I L+GG ASGF
Sbjct: 71 KDTSQDEAGTAAVKTVEL-DAALGGRAVQYREIQGHESDKFLSYFKPCIIPLEGGVASGF 129
Query: 424 SHVTINEGTEKRLFQIKGKRNVRVKQV 504
E E RL+ KGKR V +KQV
Sbjct: 130 KKPE-EEEFETRLYTCKGKRAVHLKQV 155
Score = 53.6 bits (123), Expect = 8e-08
Identities = 34/119 (28%), Positives = 57/119 (47%), Gaps = 1/119 (0%)
Frame = +1
Query: 97 LEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDKDRL-TWDIHFWLGSRTSQDEAGA 273
LE+W I+ + K G YSGD Y+VL T +R + + W G ++Q++
Sbjct: 394 LEVWYIDANSKTVLSKDHVGKLYSGDCYLVLYTYHSGERKEDYFLCCWFGKNSNQEDQET 453
Query: 274 AAILTVNLDDEQFQGSAVQHREVQYYESKEFLEYFSPAIRYLKGGHASGFSHVTINEGT 450
A L + + +G VQ R + E +F+ F + LKGG +SG+ + +G+
Sbjct: 454 AVRLASTMTN-SLKGRPVQARIFEGKEPPQFVALFQHMV-VLKGGLSSGYKNSMTEKGS 510
>At5g57320.1 68418.m07160 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 962
Score = 161 bits (390), Expect = 3e-40
Identities = 83/153 (54%), Positives = 106/153 (69%), Gaps = 1/153 (0%)
Frame = +1
Query: 49 MPEVHQAFADAGRKPGLEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDKD-RLTWD 225
M ++ QA AG+K G+EIWRIENF+PV VP+ G F++GDSYIVL TT + L D
Sbjct: 5 MRDLDQALQGAGQKSGIEIWRIENFKPVTVPQESHGKFFTGDSYIVLKTTASRSGSLHHD 64
Query: 226 IHFWLGSRTSQDEAGAAAILTVNLDDEQFQGSAVQHREVQYYESKEFLEYFSPAIRYLKG 405
IH+WLG +SQDEAGA A++TV L D G AVQ+REVQ +E+++FL YF P I +G
Sbjct: 65 IHYWLGKDSSQDEAGAVAVMTVEL-DSALGGRAVQYREVQGHETEKFLSYFKPCIIPQEG 123
Query: 406 GHASGFSHVTINEGTEKRLFQIKGKRNVRVKQV 504
G ASGF+HV E + RL+ KGK VRVK+V
Sbjct: 124 GVASGFNHVKPEE-HQTRLYICKGKHVVRVKEV 155
Score = 44.8 bits (101), Expect = 3e-05
Identities = 36/138 (26%), Positives = 57/138 (41%)
Frame = +1
Query: 97 LEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDKDRLTWDIHFWLGSRTSQDEAGAA 276
L++WRI E + + + FYSGD YI+ + +DR + W G + S +E A+
Sbjct: 396 LQVWRINCEEKILLEAAEQSKFYSGDCYILQYSYPGEDREEHLVGTWFG-KQSVEEDRAS 454
Query: 277 AILTVNLDDEQFQGSAVQHREVQYYESKEFLEYFSPAIRYLKGGHASGFSHVTINEGTEK 456
AI N E + Q R + E +F I + KGG + F
Sbjct: 455 AISLANKMVESMKFVPAQARINEGKEPIQFFVIMQSFITF-KGGVSDAFKKYIAENDIPD 513
Query: 457 RLFQIKGKRNVRVKQVKP 510
++ +G RV+ P
Sbjct: 514 TTYEAEGVALFRVQGSGP 531
>At4g30160.1 68417.m04289 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 974
Score = 157 bits (380), Expect = 5e-39
Identities = 83/153 (54%), Positives = 101/153 (66%), Gaps = 1/153 (0%)
Frame = +1
Query: 49 MPEVHQAFADAGRKPGLEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDK-DRLTWD 225
M ++ AF AG+K G+EIWRIENF P +PK+ G F++GDSYIVL TT K L D
Sbjct: 5 MRDLDPAFQGAGQKAGIEIWRIENFIPTPIPKSSIGKFFTGDSYIVLKTTALKTGALRHD 64
Query: 226 IHFWLGSRTSQDEAGAAAILTVNLDDEQFQGSAVQHREVQYYESKEFLEYFSPAIRYLKG 405
IH+WLG TSQDEAG AA+ TV L D G AVQ+REVQ +E+++FL YF P I +G
Sbjct: 65 IHYWLGKDTSQDEAGTAAVKTVEL-DAALGGRAVQYREVQGHETEKFLSYFKPCIIPQEG 123
Query: 406 GHASGFSHVTINEGTEKRLFQIKGKRNVRVKQV 504
G ASGF HV E RLF +GK V VK+V
Sbjct: 124 GVASGFKHVVAEEHI-TRLFVCRGKHVVHVKEV 155
Score = 46.4 bits (105), Expect = 1e-05
Identities = 33/154 (21%), Positives = 64/154 (41%), Gaps = 2/154 (1%)
Frame = +1
Query: 55 EVHQAFADAGRKPGLEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDKDRLTWDIHF 234
E Q F D L++WR+ + FYSGD Y+ + +++ I
Sbjct: 382 EEPQVFIDC--TGNLQVWRVNGQAKTLLQAADHSKFYSGDCYVFQYSYPGEEKEEVLIGT 439
Query: 235 WLGSRTSQDEAGAAAILTVNLDDEQFQGSAVQHREVQYYESKEFLEYF--SPAIRYLKGG 408
W G ++ ++E G+A V++ + + + + YE KE +++F + KGG
Sbjct: 440 WFGKQSVEEERGSA----VSMASKMVESMKFVPAQARIYEGKEPIQFFVIMQSFIVFKGG 495
Query: 409 HASGFSHVTINEGTEKRLFQIKGKRNVRVKQVKP 510
+SG+ + + + G R++ P
Sbjct: 496 ISSGYKKYIAEKEVDDDTYNENGVALFRIQGSGP 529
>At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to villin
1 (VLN1) [Arabidopsis thaliana] GI:3415113
Length = 909
Score = 93.9 bits (223), Expect = 6e-20
Identities = 54/151 (35%), Positives = 87/151 (57%), Gaps = 1/151 (0%)
Frame = +1
Query: 55 EVHQAFADAGRKPGLEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDK-DRLTWDIH 231
++ AF G K GLEIW + N + +++PK+ FG F+SG++Y+VL T K + +DIH
Sbjct: 7 DIDSAFQGVGTKSGLEIWCVYNKQLISIPKSSFGKFHSGNAYLVLRTFLRKIESPQYDIH 66
Query: 232 FWLGSRTSQDEAGAAAILTVNLDDEQFQGSAVQHREVQYYESKEFLEYFSPAIRYLKGGH 411
+WLG ++ ++ A+ ++L D VQ+REVQ E+++FL YF P I ++G +
Sbjct: 67 YWLGIDANEVDSILASDKALDL-DAALGCCTVQYREVQGQETEKFLSYFKPCIIPVEGKY 125
Query: 412 ASGFSHVTINEGTEKRLFQIKGKRNVRVKQV 504
+ E + L + KG VRVK+V
Sbjct: 126 SPKTG--IAGETYQVTLLRCKGDHVVRVKEV 154
Score = 32.3 bits (70), Expect = 0.20
Identities = 24/107 (22%), Positives = 45/107 (42%)
Frame = +1
Query: 88 KPGLEIWRIENFEPVAVPKTQFGLFYSGDSYIVLNTTGDKDRLTWDIHFWLGSRTSQDEA 267
+ L++WR++ + + ++GD Y+V K+R ++ W+G + Q +
Sbjct: 388 RDNLKVWRVDGDDVSLLSIPDQTKLFTGDCYLVQYKYTYKERTEHLLYVWIGCESIQQDR 447
Query: 268 GAAAILTVNLDDEQFQGSAVQHREVQYYESKEFLEYFSPAIRYLKGG 408
A AI + +G +V Q E F F + + KGG
Sbjct: 448 -ADAITNASAIVGTTKGESVLCHIYQGNEPSRFFPMFQSLVVF-KGG 492
>At1g09390.1 68414.m01050 GDSL-motif lipase/hydrolase family protein
Similar to early nodulin ENOD8 [Medicago sativa]
GI:304037, lanatoside 15'-O-acetylesterase [Digitalis
lanata] GI:3688284, elicitor-induced glycoprotein iEP4
[Daucus carota] GI:1911765; contains InterPro Entry
IPR001087 Lipolytic enzyme, G-D-S-L family
Length = 370
Score = 29.1 bits (62), Expect = 1.9
Identities = 13/42 (30%), Positives = 22/42 (52%)
Frame = -1
Query: 141 RNGDRFEVLNPPYFETRLPAGIRECLVYFRHFRSRALSLTTL 16
+NG F ++ +P + L+ F HF+SRAL L ++
Sbjct: 107 QNGANFAIVGSSTLPRYVPFALNIQLMQFLHFKSRALELASI 148
>At1g04470.1 68414.m00438 expressed protein EST gb|ATTS5672 comes
from this gene
Length = 1035
Score = 29.1 bits (62), Expect = 1.9
Identities = 25/86 (29%), Positives = 38/86 (44%)
Frame = +2
Query: 89 SRVSKYGGLRTSNRSPFRRLSSVSSTPGTPTLY*TPQAIRTV*HGTSISGSARGPARTKP 268
SRV + GL+ RSP RR+S+V + G + +P ++ G+ G P
Sbjct: 107 SRVKRALGLKMLKRSPSRRMSTVGTVVGAVSAPSSPGNNGSIGSGSGHFSPGAGFFTVPP 166
Query: 269 ARRPSSR*TWTTNNSRDQRYSTERSN 346
AR R + R Q TE+S+
Sbjct: 167 AR--PRRPLTSAEIMRQQMKVTEQSD 190
>At2g33420.1 68415.m04096 expressed protein
Length = 1039
Score = 28.7 bits (61), Expect = 2.5
Identities = 13/31 (41%), Positives = 19/31 (61%)
Frame = +2
Query: 89 SRVSKYGGLRTSNRSPFRRLSSVSSTPGTPT 181
SRV + GL+ RSP RR+S++ + G T
Sbjct: 113 SRVKRALGLKMLKRSPSRRMSTIGAAGGAAT 143
>At5g45610.1 68418.m05605 expressed protein
Length = 633
Score = 28.3 bits (60), Expect = 3.2
Identities = 11/28 (39%), Positives = 16/28 (57%)
Frame = -1
Query: 165 VEETELSLRNGDRFEVLNPPYFETRLPA 82
++ E +L+ D + L PPY T LPA
Sbjct: 16 IDSIETTLKKADMYRPLPPPYLPTFLPA 43
>At1g68750.1 68414.m07859 phosphoenolpyruvate carboxylase family
protein / PEP carboxylase family protein similar to
SP|P51059 Phosphoenolpyruvate carboxylase 2 (EC
4.1.1.31) (PEPCASE) {Zea mays}; contains Pfam profile
PF00311: phosphoenolpyruvate carboxylase
Length = 1032
Score = 28.3 bits (60), Expect = 3.2
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = +1
Query: 304 EQFQGSAVQHREVQYYESKEFLEYFSPA 387
E+ G + QH YE+ EFL YF A
Sbjct: 812 EEISGISCQHYRSTVYENPEFLSYFHEA 839
>At1g56670.1 68414.m06517 GDSL-motif lipase/hydrolase family protein
similarity to early early nodulin ENOD8 [Medicago
sativa] GI:304037, lanatoside 15'-O-acetylesterase
[Digitalis lanata] GI:3688284, elicitor-induced
glycoprotein iEP4 [Daucus carota] GI:1911765; contains
InterPro Entry IPR001087 Lipolytic enzyme, G-D-S-L
family
Length = 373
Score = 28.3 bits (60), Expect = 3.2
Identities = 12/41 (29%), Positives = 20/41 (48%)
Frame = -1
Query: 141 RNGDRFEVLNPPYFETRLPAGIRECLVYFRHFRSRALSLTT 19
+NG F + P +P + + F HF+SR+L L +
Sbjct: 110 QNGANFAIAGSPTLPKNVPFSLNIQVKQFSHFKSRSLELAS 150
>At5g10520.1 68418.m01218 protein kinase family protein contains
protein kinase domain, INTERPRO:IPR000719
Length = 467
Score = 27.9 bits (59), Expect = 4.3
Identities = 15/39 (38%), Positives = 18/39 (46%)
Frame = +1
Query: 334 REVQYYESKEFLEYFSPAIRYLKGGHASGFSHVTINEGT 450
R Y E +YF+P KGGHA + V IN T
Sbjct: 139 RNFTYEELAVATDYFNPENMIGKGGHAEVYKGVLINGET 177
>At5g29613.1 68418.m03639 hypothetical protein
Length = 117
Score = 27.5 bits (58), Expect = 5.7
Identities = 16/63 (25%), Positives = 29/63 (46%)
Frame = +1
Query: 295 LDDEQFQGSAVQHREVQYYESKEFLEYFSPAIRYLKGGHASGFSHVTINEGTEKRLFQIK 474
LD Q Q H + ++ + F E F + A+G + + + +GT+ R +I
Sbjct: 21 LDGIQTQNGLQHHMSYKKFKDETF-EEFDDLKMVFEDNIATGGNAIGLGDGTDARTCEIA 79
Query: 475 GKR 483
GK+
Sbjct: 80 GKK 82
>At3g23270.1 68416.m02933 regulator of chromosome condensation
(RCC1) family protein contains Pfam domain PF00415:
Regulator of chromosome condensation (RCC1); similar to
zinc finger protein (GI:15811367) [Arabidopsis
thaliana]; similar to chromosome condensation regulator
protein (GI:22770461) [Cicer arietinum]
Length = 1045
Score = 27.5 bits (58), Expect = 5.7
Identities = 11/22 (50%), Positives = 16/22 (72%)
Frame = -2
Query: 131 TGSKFSILHISRPGFLPASANA 66
+ +K S++ SRPGF P S+NA
Sbjct: 701 SANKNSVMSSSRPGFTPESSNA 722
>At5g49430.1 68418.m06116 transducin family protein / WD-40 repeat
family protein similar to WD-repeat protein 9
(SP:Q9NSI6) {Homo sapiens}; contains Pfam PF00400: WD
domain, G-beta repeat (4 copies)
Length = 1677
Score = 27.1 bits (57), Expect = 7.5
Identities = 13/33 (39%), Positives = 18/33 (54%), Gaps = 1/33 (3%)
Frame = -2
Query: 140 GTATGSKFSILHISRPGFLPASANAW-CTSGIL 45
G A GSKFS+ S+ +P N+W C I+
Sbjct: 457 GCAAGSKFSVTDYSKDENVPKFKNSWFCHDNIV 489
>At1g13770.1 68414.m01616 expressed protein contains Pfam domain,
PF04884: Protein of unknown function, DUF647; contains
non-consensus (GC) splice site at intron 2
Length = 440
Score = 27.1 bits (57), Expect = 7.5
Identities = 18/46 (39%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
Frame = -2
Query: 326 TADPWNCSSSKFTVRMAAAPASSWLVLEPSQKWMSHV-RRSLSPVV 192
T + WN SSS + A ASS L ++ S +HV RR L V
Sbjct: 13 TLEEWNGSSSTKLFKTATITASSSLSIQRSANRFNHVWRRVLQAFV 58
>At3g53320.1 68416.m05883 expressed protein
Length = 553
Score = 26.6 bits (56), Expect = 9.9
Identities = 15/48 (31%), Positives = 28/48 (58%)
Frame = +2
Query: 218 HGTSISGSARGPARTKPARRPSSR*TWTTNNSRDQRYSTERSNITSPR 361
HG+S S S + +T+PAR P +TN+ + S++ +++SP+
Sbjct: 494 HGSSSSASKKSGGKTQPARSPIQE---STNSK--SKASSKVVSVSSPK 536
>At3g02750.1 68416.m00267 protein phosphatase 2C family protein /
PP2C family protein similar to protein phosphatase-2C;
PP2C (GI:3643088) [Mesembryanthemum crystallinum];
contains Pfam PF00481 : Protein phosphatase 2C domain;
Length = 492
Score = 26.6 bits (56), Expect = 9.9
Identities = 24/84 (28%), Positives = 38/84 (45%), Gaps = 7/84 (8%)
Frame = +1
Query: 52 PEVHQAFADAGRKPGLEIWR------IENFEPVAVPKTQFGLFYSGDSYIVLNTTGDKDR 213
PEV + + PGL + R +++F ++VP F D +IVL T G D
Sbjct: 284 PEVCRVWLPNCDSPGLAMARAFGDFCLKDFGLISVPDVSFRQLTEKDEFIVLATDGIWDV 343
Query: 214 LT-WDIHFWLGSRTSQDEAGAAAI 282
L+ D+ + S S+ A A +
Sbjct: 344 LSNEDVVAIVASAPSRSSAARALV 367
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,580,881
Number of Sequences: 28952
Number of extensions: 241771
Number of successful extensions: 758
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 724
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 744
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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