BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30457 (516 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g17310.2 68418.m02028 UTP--glucose-1-phosphate uridylyltransf... 97 5e-21 At3g03250.1 68416.m00321 UTP--glucose-1-phosphate uridylyltransf... 97 6e-21 At3g27470.1 68416.m03433 expressed protein contains Pfam profile... 31 0.35 At3g23210.1 68416.m02926 basic helix-loop-helix (bHLH) family pr... 31 0.35 At5g17310.1 68418.m02027 UTP--glucose-1-phosphate uridylyltransf... 29 1.4 At5g37370.2 68418.m04490 pre-mRNA splicing factor PRP38 family p... 29 1.9 At5g37370.1 68418.m04489 pre-mRNA splicing factor PRP38 family p... 29 1.9 At3g22100.1 68416.m02788 basic helix-loop-helix (bHLH) family pr... 28 3.2 At4g04740.1 68417.m00695 calcium-dependent protein kinase, putat... 28 4.3 At4g04710.1 68417.m00692 calcium-dependent protein kinase, putat... 28 4.3 At2g22140.1 68415.m02630 expressed protein ; expression supporte... 28 4.3 At4g38070.1 68417.m05377 bHLH family protein contains Pfam profi... 27 5.7 At4g21940.1 68417.m03174 calcium-dependent protein kinase, putat... 27 5.7 At4g04720.1 68417.m00693 calcium-dependent protein kinase, putat... 27 5.7 At2g01260.2 68415.m00039 expressed protein 27 5.7 At2g01260.1 68415.m00038 expressed protein 27 5.7 At1g78230.1 68414.m09116 leucine-rich repeat family protein 27 7.5 At1g31070.2 68414.m03804 UDP-N-acetylglucosamine pyrophosphoryla... 27 7.5 At4g32760.1 68417.m04661 VHS domain-containing protein / GAT dom... 27 9.9 At4g18740.2 68417.m02770 expressed protein 27 9.9 At4g18740.1 68417.m02769 expressed protein 27 9.9 At4g08710.1 68417.m01439 hypothetical protein contains Pfam prof... 27 9.9 At2g35020.1 68415.m04296 UTP--glucose-1-phosphate uridylyltransf... 27 9.9 >At5g17310.2 68418.m02028 UTP--glucose-1-phosphate uridylyltransferase, putative / UDP-glucose pyrophosphorylase, putative / UGPase, putative strong similarity to SP|P19595 UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) (UDP-glucose pyrophosphorylase) (UDPGP) (UGPase) {Solanum tuberosum}; contains Pfam profile PF01704: UTP--glucose-1-phosphate uridylyltransferase Length = 470 Score = 97.5 bits (232), Expect = 5e-21 Identities = 48/96 (50%), Positives = 65/96 (67%), Gaps = 2/96 (2%) Frame = +2 Query: 233 EKEFKGFKTLFSRFLAEQGPSVTWEKIEKLPEGAVIDYNSLSTPTTD--NVHHMLDKLVV 406 E E GF L SR+L+ + + W KI+ + V+ Y+ ++ + D ++LDKLVV Sbjct: 24 ENEKSGFINLVSRYLSGEAQHIEWSKIQTPTDEIVVPYDKMANVSEDASETKYLLDKLVV 83 Query: 407 VKLNGGLGTSMGCKGPKSVIQVRNDLTFLDLTVQQI 514 +KLNGGLGT+MGC GPKSVI+VR+ LTFLDL V QI Sbjct: 84 LKLNGGLGTTMGCTGPKSVIEVRDGLTFLDLIVIQI 119 >At3g03250.1 68416.m00321 UTP--glucose-1-phosphate uridylyltransferase, putative / UDP-glucose pyrophosphorylase, putative / UGPase, putative strong similarity to SP|P19595 UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) (UDP-glucose pyrophosphorylase) (UDPGP) (UGPase) {Solanum tuberosum}; contains Pfam profile PF01704: UTP--glucose-1-phosphate uridylyltransferase Length = 469 Score = 97.1 bits (231), Expect = 6e-21 Identities = 50/97 (51%), Positives = 67/97 (69%), Gaps = 3/97 (3%) Frame = +2 Query: 233 EKEFKGFKTLFSRFLAEQGPSVTWEKIEKLPEGAVIDYNSLSTPTTDNV---HHMLDKLV 403 E E GF +L SR+L+ + + W KI+ + V+ Y + TP + +V ++LDKLV Sbjct: 23 ESEKSGFISLVSRYLSGEAQHIEWSKIQTPTDEIVVPYEKM-TPVSQDVAETKNLLDKLV 81 Query: 404 VVKLNGGLGTSMGCKGPKSVIQVRNDLTFLDLTVQQI 514 V+KLNGGLGT+MGC GPKSVI+VR+ LTFLDL V QI Sbjct: 82 VLKLNGGLGTTMGCTGPKSVIEVRDGLTFLDLIVIQI 118 >At3g27470.1 68416.m03433 expressed protein contains Pfam profile PF05212: Protein of unknown function (DUF707) Length = 398 Score = 31.5 bits (68), Expect = 0.35 Identities = 17/56 (30%), Positives = 25/56 (44%) Frame = -1 Query: 483 RSFRTWITDLGPLQPMDVPRPPLSFTTTNLSSIWCTLSVVGVDRLL*SITAPSGSF 316 R F +W + G D+P PP ++ T IW + + G +RL I P F Sbjct: 62 RLFGSWSSTKGLKLKNDIPDPPYNYNDT---KIWVSTNPRGAERLPPDIVTPESDF 114 >At3g23210.1 68416.m02926 basic helix-loop-helix (bHLH) family protein similar to hypothetical protein GB:CAB10220 from [Arabidopsis thaliana] Length = 320 Score = 31.5 bits (68), Expect = 0.35 Identities = 12/28 (42%), Positives = 18/28 (64%) Frame = -3 Query: 217 GFRGRGDELLQLHLQSSEGITFGGLLEV 134 GFRG+ D+ HLQ +G F G++E+ Sbjct: 107 GFRGQNDDSAVPHLQQQQGQVFSGVVEI 134 >At5g17310.1 68418.m02027 UTP--glucose-1-phosphate uridylyltransferase, putative / UDP-glucose pyrophosphorylase, putative / UGPase, putative strong similarity to SP|P19595 UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) (UDP-glucose pyrophosphorylase) (UDPGP) (UGPase) {Solanum tuberosum}; contains Pfam profile PF01704: UTP--glucose-1-phosphate uridylyltransferase Length = 390 Score = 29.5 bits (63), Expect = 1.4 Identities = 14/20 (70%), Positives = 17/20 (85%) Frame = +2 Query: 455 KSVIQVRNDLTFLDLTVQQI 514 +SVI+VR+ LTFLDL V QI Sbjct: 20 RSVIEVRDGLTFLDLIVIQI 39 >At5g37370.2 68418.m04490 pre-mRNA splicing factor PRP38 family protein (SRL1) contains Pfam profile PF03371: PRP38 family Length = 385 Score = 29.1 bits (62), Expect = 1.9 Identities = 16/48 (33%), Positives = 23/48 (47%) Frame = +1 Query: 283 TGSVGNMGENREASRRSCNRL*QSVHANDGQRAPYARQVGGGEA*RRP 426 +GS G+M E + R + S+ + GQRAP+ G RRP Sbjct: 172 SGSTGDMTRGSEDTARRPPSVKASLSVSFGQRAPHRASTRGSSPVRRP 219 >At5g37370.1 68418.m04489 pre-mRNA splicing factor PRP38 family protein (SRL1) contains Pfam profile PF03371: PRP38 family Length = 393 Score = 29.1 bits (62), Expect = 1.9 Identities = 16/48 (33%), Positives = 23/48 (47%) Frame = +1 Query: 283 TGSVGNMGENREASRRSCNRL*QSVHANDGQRAPYARQVGGGEA*RRP 426 +GS G+M E + R + S+ + GQRAP+ G RRP Sbjct: 180 SGSTGDMTRGSEDTARRPPSVKASLSVSFGQRAPHRASTRGSSPVRRP 227 >At3g22100.1 68416.m02788 basic helix-loop-helix (bHLH) family protein contains Pfam profile: PF00010 helix-loop-helix DNA-binding domain Length = 252 Score = 28.3 bits (60), Expect = 3.2 Identities = 22/74 (29%), Positives = 35/74 (47%) Frame = +2 Query: 101 AVTSGTPSGSRDFKEATKRDALARLEVELEKLISTAPETRRPQLEKEFKGFKTLFSRFLA 280 + TSG+P+ S D TKR ++ LEKL+ P R+ L + + +FL Sbjct: 125 STTSGSPTASNDGGIITKRRKISDKIRSLEKLM---PWERKMNLAMTLEE-SHKYIKFLQ 180 Query: 281 EQGPSVTWEKIEKL 322 Q S+ W +E + Sbjct: 181 SQIASLRWMPLESV 194 >At4g04740.1 68417.m00695 calcium-dependent protein kinase, putative / CDPK, putative similar to calcium-dependent protein kinase [Lycopersicon esculentum] gi|19171502|emb|CAC87494 Length = 520 Score = 27.9 bits (59), Expect = 4.3 Identities = 10/28 (35%), Positives = 19/28 (67%) Frame = +2 Query: 233 EKEFKGFKTLFSRFLAEQGPSVTWEKIE 316 E+E KG KTLF+ + ++T+E+++ Sbjct: 368 EEEIKGLKTLFANMDTNRSGTITYEQLQ 395 >At4g04710.1 68417.m00692 calcium-dependent protein kinase, putative / CDPK, putative similar to calcium-dependent protein kinase [Nicotiana tabacum] gi|3283996|gb|AAC25423; contains protein kinase domain, Pfam:PF00069; contains EF hand domain (calcium-binding EF-hand), Pfam:PF00036, INTERPRO:IPR002048 Length = 575 Score = 27.9 bits (59), Expect = 4.3 Identities = 10/28 (35%), Positives = 19/28 (67%) Frame = +2 Query: 233 EKEFKGFKTLFSRFLAEQGPSVTWEKIE 316 E+E KG KT+F ++ S+T+E+++ Sbjct: 331 EEEIKGLKTMFENMDMDKSGSITYEELK 358 >At2g22140.1 68415.m02630 expressed protein ; expression supported by MPSS Length = 506 Score = 27.9 bits (59), Expect = 4.3 Identities = 21/63 (33%), Positives = 28/63 (44%) Frame = +2 Query: 59 KK*SQKPTTPIKRFAVTSGTPSGSRDFKEATKRDALARLEVELEKLISTAPETRRPQLEK 238 KK + T P+ A+ S D A + L + + LEK S A E R +LEK Sbjct: 166 KKKIRTTTLPVPGEALPKKRQS-KEDKTSAMEEKKLRKEQERLEKAASKAEEAERKRLEK 224 Query: 239 EFK 247 E K Sbjct: 225 EKK 227 >At4g38070.1 68417.m05377 bHLH family protein contains Pfam profile: PF00010 helix-loop-helix DNA-binding domain; PMID: 12679534; putative bHLH131 transcription factor Length = 1513 Score = 27.5 bits (58), Expect = 5.7 Identities = 16/54 (29%), Positives = 26/54 (48%) Frame = +2 Query: 110 SGTPSGSRDFKEATKRDALARLEVELEKLISTAPETRRPQLEKEFKGFKTLFSR 271 SG S + +++ + R E+E K + ET+R + EK KG K + R Sbjct: 115 SGLDEASEKNIDLEQKNNVYRAEIEGLKGLLAVAETKRIEAEKTVKGMKEMRGR 168 >At4g21940.1 68417.m03174 calcium-dependent protein kinase, putative / CDPK, putative similar to calcium-dependent protein kinase [Nicotiana tabacum] gi|3283996|gb|AAC25423 Length = 554 Score = 27.5 bits (58), Expect = 5.7 Identities = 9/28 (32%), Positives = 20/28 (71%) Frame = +2 Query: 233 EKEFKGFKTLFSRFLAEQGPSVTWEKIE 316 E+E KG KT+F+ ++ ++T+E+++ Sbjct: 401 EEEIKGLKTMFANMDTDKSGTITYEELK 428 >At4g04720.1 68417.m00693 calcium-dependent protein kinase, putative / CDPK, putative similar to calcium-dependent protein kinase(CDPK) [Carrot] SWISS-PROT:P28582 Length = 531 Score = 27.5 bits (58), Expect = 5.7 Identities = 9/28 (32%), Positives = 20/28 (71%) Frame = +2 Query: 233 EKEFKGFKTLFSRFLAEQGPSVTWEKIE 316 E+E KG KT+F+ ++ ++T+E+++ Sbjct: 379 EEEIKGLKTMFANIDTDKSGTITYEELK 406 >At2g01260.2 68415.m00039 expressed protein Length = 324 Score = 27.5 bits (58), Expect = 5.7 Identities = 24/76 (31%), Positives = 34/76 (44%), Gaps = 4/76 (5%) Frame = +2 Query: 140 KEATKR-DALARLEVELEKLISTAPETRRPQLEKEFKGFKTLFSRFLAEQGPSVTWEKIE 316 + A +R D L R + ++ + S+AP + QLE L RFL PSV + + Sbjct: 25 RRANQRIDQLRRAQSDVSNVPSSAPSPHKQQLEPSDLSSSNL-DRFLESVTPSVPAQFLS 83 Query: 317 KL---PEGAVIDYNSL 355 K A DYN L Sbjct: 84 KTLLRERRADDDYNKL 99 >At2g01260.1 68415.m00038 expressed protein Length = 369 Score = 27.5 bits (58), Expect = 5.7 Identities = 24/76 (31%), Positives = 34/76 (44%), Gaps = 4/76 (5%) Frame = +2 Query: 140 KEATKR-DALARLEVELEKLISTAPETRRPQLEKEFKGFKTLFSRFLAEQGPSVTWEKIE 316 + A +R D L R + ++ + S+AP + QLE L RFL PSV + + Sbjct: 25 RRANQRIDQLRRAQSDVSNVPSSAPSPHKQQLEPSDLSSSNL-DRFLESVTPSVPAQFLS 83 Query: 317 KL---PEGAVIDYNSL 355 K A DYN L Sbjct: 84 KTLLRERRADDDYNKL 99 >At1g78230.1 68414.m09116 leucine-rich repeat family protein Length = 676 Score = 27.1 bits (57), Expect = 7.5 Identities = 12/18 (66%), Positives = 13/18 (72%) Frame = -1 Query: 270 LLKSVLKPLNSFSSCGRL 217 LL SV PL+ FS CGRL Sbjct: 18 LLSSVFCPLSLFSGCGRL 35 >At1g31070.2 68414.m03804 UDP-N-acetylglucosamine pyrophosphorylase-related low similarity to SP|P43123 UDP-N-acetylglucosamine pyrophosphorylase (EC 2.7.7.23) {Saccharomyces cerevisiae} Length = 505 Score = 27.1 bits (57), Expect = 7.5 Identities = 12/26 (46%), Positives = 15/26 (57%) Frame = +2 Query: 395 KLVVVKLNGGLGTSMGCKGPKSVIQV 472 KL VV L+GG GT +G PK + Sbjct: 128 KLGVVLLSGGQGTRLGSSDPKGCFNI 153 >At4g32760.1 68417.m04661 VHS domain-containing protein / GAT domain-containing protein weak similarity to hepatocyte growth factor-regulated tyrosine kinase substrate HRS isoform 2 [Homo sapiens] GI:9022389; contains Pfam profiles PF00790: VHS domain, PF03127: GAT domain Length = 838 Score = 26.6 bits (56), Expect = 9.9 Identities = 12/36 (33%), Positives = 17/36 (47%) Frame = +1 Query: 7 LKLCLCCYQSLLSRLLHKEMKPETNDSDKKIRSHQR 114 L+ C CCY SL L +E + + + K R R Sbjct: 41 LRFCFCCYLSLSLYLFERERERVNSKMEIKFRFFTR 76 >At4g18740.2 68417.m02770 expressed protein Length = 214 Score = 26.6 bits (56), Expect = 9.9 Identities = 15/38 (39%), Positives = 22/38 (57%), Gaps = 1/38 (2%) Frame = -1 Query: 498 RSRKVRSFRTWITDLGPLQPMDVPRPPLSFT-TTNLSS 388 +SRK T + + P + +++PRPP SF T LSS Sbjct: 142 KSRKKTEGDTSVKEKPPKRQVELPRPPSSFVKRTPLSS 179 >At4g18740.1 68417.m02769 expressed protein Length = 245 Score = 26.6 bits (56), Expect = 9.9 Identities = 15/38 (39%), Positives = 22/38 (57%), Gaps = 1/38 (2%) Frame = -1 Query: 498 RSRKVRSFRTWITDLGPLQPMDVPRPPLSFT-TTNLSS 388 +SRK T + + P + +++PRPP SF T LSS Sbjct: 142 KSRKKTEGDTSVKEKPPKRQVELPRPPSSFVKRTPLSS 179 >At4g08710.1 68417.m01439 hypothetical protein contains Pfam profile PF03384: Drosophila protein of unknown function, DUF287 Length = 715 Score = 26.6 bits (56), Expect = 9.9 Identities = 13/34 (38%), Positives = 18/34 (52%) Frame = +2 Query: 122 SGSRDFKEATKRDALARLEVELEKLISTAPETRR 223 +G+ DFK +D + + E EKLI PE R Sbjct: 297 TGNLDFKRKHFKDTVVKREDVREKLIGMVPEGER 330 >At2g35020.1 68415.m04296 UTP--glucose-1-phosphate uridylyltransferase family protein similar to SP|Q16222 UDP-N-acetylhexosamine pyrophosphorylase (Antigen X) {Homo sapiens}; contains Pfam profile PF01704: UTP--glucose-1-phosphate uridylyltransferase Length = 502 Score = 26.6 bits (56), Expect = 9.9 Identities = 12/26 (46%), Positives = 15/26 (57%) Frame = +2 Query: 395 KLVVVKLNGGLGTSMGCKGPKSVIQV 472 KL VV L+GG GT +G PK + Sbjct: 124 KLGVVLLSGGQGTRLGSSDPKGCYNI 149 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,774,268 Number of Sequences: 28952 Number of extensions: 252109 Number of successful extensions: 857 Number of sequences better than 10.0: 23 Number of HSP's better than 10.0 without gapping: 814 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 853 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 937669760 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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