BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30446 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 31 0.10 SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam... 28 0.72 SPCC645.12c |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 28 0.72 SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr 1||... 25 6.7 SPAC3H8.07c |||prefoldin subunit 3|Schizosaccharomyces pombe|chr... 25 8.9 SPAC26A3.15c |nsp1||nucleoporin Nsp1|Schizosaccharomyces pombe|c... 25 8.9 >SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 1752 Score = 31.1 bits (67), Expect = 0.10 Identities = 31/82 (37%), Positives = 40/82 (48%), Gaps = 8/82 (9%) Frame = -2 Query: 440 YLGM*APSATKPFSSAV*STL*VT-----PSGPTYS*NP--LTLMPSFSVPTFSTLPASS 282 Y G+ +P T PFSSA+ +T PS P YS +P + PS+S + S P S Sbjct: 1531 YKGVQSPGYTSPFSSAMSPGYGLTSPSYSPSSPGYSTSPAYMPSSPSYSPTSPSYSPTSP 1590 Query: 281 ARILFSVSYK*W*PS-GATSLS 219 + S SY PS ATS S Sbjct: 1591 SYSPTSPSYSPTSPSYSATSPS 1612 >SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1082 Score = 28.3 bits (60), Expect = 0.72 Identities = 22/66 (33%), Positives = 35/66 (53%), Gaps = 1/66 (1%) Frame = -2 Query: 419 SATKPFSSAV*STL*VTPSGPTYS*NPLTL-MPSFSVPTFSTLPASSARILFSVSYK*W* 243 ++T SS+ S++ +P+G S TL M S P FS + S+++ SVSY Sbjct: 130 TSTVDISSSTSSSVINSPTGTAVSSQISTLSMSPSSTPVFSPSASVSSKVASSVSYVSSE 189 Query: 242 PSGATS 225 PS ++S Sbjct: 190 PSDSSS 195 >SPCC645.12c |||sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 198 Score = 28.3 bits (60), Expect = 0.72 Identities = 11/19 (57%), Positives = 14/19 (73%) Frame = +1 Query: 220 DNDVAPEGYHYLYETENKI 276 DND+ PE Y LYE E+K+ Sbjct: 132 DNDLEPEVYDILYEEESKL 150 >SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1116 Score = 25.0 bits (52), Expect = 6.7 Identities = 12/35 (34%), Positives = 19/35 (54%) Frame = +2 Query: 251 TCTRPRTRFSLKKPARSRTLAPKTKASRSRDSTNT 355 T RP + +LK P S + AP++ +S + S T Sbjct: 500 TVCRPHKKVTLKMPLNSGSSAPQSPSSNTSASVLT 534 >SPAC3H8.07c |||prefoldin subunit 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 169 Score = 24.6 bits (51), Expect = 8.9 Identities = 12/31 (38%), Positives = 17/31 (54%) Frame = -1 Query: 393 SVVYSVGDTVGANVFVESLDLDAFVFGANVL 301 +V Y + DT+ A VE+ D GANV+ Sbjct: 79 TVTYELNDTLNAKAEVEAKDNVYLWLGANVM 109 >SPAC26A3.15c |nsp1||nucleoporin Nsp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 598 Score = 24.6 bits (51), Expect = 8.9 Identities = 16/47 (34%), Positives = 22/47 (46%), Gaps = 4/47 (8%) Frame = +2 Query: 239 KATTTCTRPRTRFSLKKPA----RSRTLAPKTKASRSRDSTNTLAPT 367 K TTT + T FS KPA ++ AP + ++ ST PT Sbjct: 286 KGTTTTSSAGTGFSFGKPATTEDTNKPTAPNSAFTKPATSTGDNKPT 332 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,741,757 Number of Sequences: 5004 Number of extensions: 30307 Number of successful extensions: 85 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 84 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 85 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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