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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30430
         (516 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC22H10.12c |gdi1|sec19|GDP dissociation inhibitor Gdi1 |Schiz...   193   1e-50
SPBC15C4.03 |||Rab geranylgeranyltransferase escort protein |Sch...    51   1e-07
SPBC713.12 |erg1||squalene monooxygenase Erg1 |Schizosaccharomyc...    33   0.033
SPAC12G12.13c |cid14||poly|Schizosaccharomyces pombe|chr 1|||Manual    27   1.3  
SPBC2G2.11 |||N-myristoyltransferase 1|Schizosaccharomyces pombe...    27   2.2  
SPAC17H9.03c |rdl1||RAD51D-like protein 1|Schizosaccharomyces po...    26   2.9  
SPAPB17E12.08 |||N-glycosylation protein |Schizosaccharomyces po...    26   3.8  
SPCC777.14 |prp4||serine/threonine protein kinase Prp4|Schizosac...    26   3.8  
SPAC17A2.05 |||fumerate reductase|Schizosaccharomyces pombe|chr ...    25   5.1  
SPAC139.04c |fap2||L-saccharopine oxidase|Schizosaccharomyces po...    25   6.7  
SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|c...    25   6.7  
SPAC3F10.16c |||GTP binding protein, HSR1-related|Schizosaccharo...    25   6.7  
SPAC8E11.05c |||conserved fungal protein|Schizosaccharomyces pom...    25   8.9  
SPBC776.12c |hsk1||serine/threonine protein kinase Hsk1|Schizosa...    25   8.9  
SPAC1F7.05 |cdc22||ribonucleoside reductase large subunit Cdc22|...    25   8.9  

>SPAC22H10.12c |gdi1|sec19|GDP dissociation inhibitor Gdi1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 440

 Score =  193 bits (471), Expect = 1e-50
 Identities = 95/149 (63%), Positives = 115/149 (77%), Gaps = 1/149 (0%)
 Frame = +1

Query: 73  MDEEYDVIVLGTGLKECILSGMLSVSGKKVLHIDRNKYYGGESASITPLEELFAKFN-AP 249
           MDEEYDVIVLGTGL EC+LSG+LSV GKKVLHIDRN YYG +SAS+  L +L+A F    
Sbjct: 1   MDEEYDVIVLGTGLTECVLSGLLSVDGKKVLHIDRNDYYGADSASLN-LTQLYALFRPGE 59

Query: 250 APDETYGRGRDWNVDLIPKFLMANGLLVKLLIHTGVTRYLEFKSIEGSYVYKGGKISKVP 429
              E+ GR RDW VDL+PKFLMANG L  +LI+T VTRY+EFK I GSYVY+ G+I+KVP
Sbjct: 60  QRPESLGRDRDWCVDLVPKFLMANGDLTNILIYTDVTRYIEFKQIAGSYVYRDGRIAKVP 119

Query: 430 VDQKEALASDLMGMFEKRRFRNFLIYVQD 516
            ++ EAL S LM +FEKRR + FL +V +
Sbjct: 120 GNEMEALKSPLMSLFEKRRAKKFLEWVNN 148


>SPBC15C4.03 |||Rab geranylgeranyltransferase escort protein
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 459

 Score = 50.8 bits (116), Expect = 1e-07
 Identities = 48/170 (28%), Positives = 74/170 (43%), Gaps = 26/170 (15%)
 Frame = +1

Query: 85  YDVIVLGTGLKECILSGMLSVSGKKVLHIDRNKYYGGESASIT--PLEELFAKFNAPAPD 258
           YDVI++GT L+  ILS  LS + ++VLHID N +YG    S+T   LE++  K       
Sbjct: 7   YDVIIVGTNLRNSILSAALSWANQRVLHIDENSFYGEIDGSLTLRDLEQINEKIKKVDSS 66

Query: 259 ETYGRG------------RDWNVDLIPK-----------FLMANGLLVKLLIHTGVTRYL 369
           +                 +  N DLIPK            + A+  LVKLL  T + +YL
Sbjct: 67  QILNDNGSHKSPLKRFEVQFLNKDLIPKNKGSVIQFHPQEIFASSELVKLLSETKIYKYL 126

Query: 370 EFKSIEG-SYVYKGGKISKVPVDQKEALASDLMGMFEKRRFRNFLIYVQD 516
             K       +    +  KVP  + +   +  + +  KR    F+ +V +
Sbjct: 127 LLKPARSFRLLTSNEEWIKVPESRADIFNNKNLSLASKRIVMRFMKFVSN 176


>SPBC713.12 |erg1||squalene monooxygenase Erg1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 457

 Score = 32.7 bits (71), Expect = 0.033
 Identities = 12/34 (35%), Positives = 22/34 (64%)
 Frame = +1

Query: 79  EEYDVIVLGTGLKECILSGMLSVSGKKVLHIDRN 180
           ++ D+I++G G+  C L   L   G+KVL ++R+
Sbjct: 4   QDADIIIIGAGITGCALGAALGRQGRKVLVLERD 37


>SPAC12G12.13c |cid14||poly|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 684

 Score = 27.5 bits (58), Expect = 1.3
 Identities = 15/57 (26%), Positives = 27/57 (47%)
 Frame = +1

Query: 151 GKKVLHIDRNKYYGGESASITPLEELFAKFNAPAPDETYGRGRDWNVDLIPKFLMAN 321
           GKK +  +RN Y   ++    PL     K + P+  ++  + +D N D   + L+ N
Sbjct: 2   GKKSVSFNRNNYKKRKNERTEPLPRRIFKNDKPSKFKSKRKEKDKNSDAYDEMLLNN 58


>SPBC2G2.11 |||N-myristoyltransferase 1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 466

 Score = 26.6 bits (56), Expect = 2.2
 Identities = 12/31 (38%), Positives = 15/31 (48%)
 Frame = +1

Query: 217 LEELFAKFNAPAPDETYGRGRDWNVDLIPKF 309
           L  L  K  A AP +TY   + W    +PKF
Sbjct: 32  LRSLIEKEEAAAPPKTYEDFKFWKTQPVPKF 62


>SPAC17H9.03c |rdl1||RAD51D-like protein 1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 230

 Score = 26.2 bits (55), Expect = 2.9
 Identities = 10/24 (41%), Positives = 18/24 (75%)
 Frame = +2

Query: 140 CRFLGRRFCTSIAISTTVVNLRQL 211
           CR L R+F  ++ +ST++V ++QL
Sbjct: 116 CRILTRKFRLAVYLSTSLVYIKQL 139


>SPAPB17E12.08 |||N-glycosylation protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 166

 Score = 25.8 bits (54), Expect = 3.8
 Identities = 13/37 (35%), Positives = 20/37 (54%)
 Frame = +1

Query: 247 PAPDETYGRGRDWNVDLIPKFLMANGLLVKLLIHTGV 357
           P P +  G  + + V     F++ANG L+K LIH  +
Sbjct: 14  PRPAQALGINQPF-VSAYLSFVLANGFLLKWLIHYSI 49


>SPCC777.14 |prp4||serine/threonine protein kinase
           Prp4|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 477

 Score = 25.8 bits (54), Expect = 3.8
 Identities = 26/86 (30%), Positives = 44/86 (51%), Gaps = 1/86 (1%)
 Frame = -1

Query: 474 KHAHKIRRKSFFLIDRYFGDFSTFVNITSFDGLKLQVPGDARVNKQFD*QSISHQKLRNQ 295
           K +HK+ ++S FL D +  DF+ F+ I   D +  Q   + R   +F   S   + +R++
Sbjct: 378 KFSHKMLKRSQFLNDHFDADFN-FIQI-DHDPITNQ---ETRKPVKF---SKPTKDIRSR 429

Query: 294 V-NVPVSTSTVCFIRCRCVELSEQLL 220
           +  VP ST     IR   ++L E+ L
Sbjct: 430 LKEVPTSTDEEFIIRQELMDLLEKCL 455


>SPAC17A2.05 |||fumerate reductase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 513

 Score = 25.4 bits (53), Expect = 5.1
 Identities = 13/36 (36%), Positives = 19/36 (52%)
 Frame = +1

Query: 94  IVLGTGLKECILSGMLSVSGKKVLHIDRNKYYGGES 201
           IV+G GL     +  +   G  VL +D+N  +GG S
Sbjct: 42  IVIGGGLAGLSATNTILDLGGNVLLLDKNTAFGGNS 77


>SPAC139.04c |fap2||L-saccharopine oxidase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 433

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 11/20 (55%), Positives = 14/20 (70%)
 Frame = -2

Query: 503 IKKLRKRLFSNMPIRSDARA 444
           I+K    LFSN P+RSD +A
Sbjct: 126 IRKKYPSLFSNSPLRSDMQA 145


>SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 649

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 11/27 (40%), Positives = 15/27 (55%)
 Frame = +2

Query: 338 CLFTRASPGTWSLSPSKEVMFTKVEKS 418
           C+F R S  TWS+  S   M  ++ KS
Sbjct: 487 CVFIRTSMQTWSMFRSTHSMKQEIAKS 513


>SPAC3F10.16c |||GTP binding protein,
           HSR1-related|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 616

 Score = 25.0 bits (52), Expect = 6.7
 Identities = 11/32 (34%), Positives = 16/32 (50%)
 Frame = +1

Query: 163 LHIDRNKYYGGESASITPLEELFAKFNAPAPD 258
           L  D N  +    A++  LE +F KF +  PD
Sbjct: 266 LQADENDVHSSRIATLKVLEGIFEKFASTLPD 297


>SPAC8E11.05c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 338

 Score = 24.6 bits (51), Expect = 8.9
 Identities = 12/35 (34%), Positives = 18/35 (51%)
 Frame = -2

Query: 179 LRSMCKTFFPETDNMPLKIHSFKPVPRTITSYSSS 75
           LRS  +  F  T  +P+ +    P P T  S++SS
Sbjct: 195 LRSSIRHIFLVTMGLPVDLDELLPTPSTQGSFNSS 229


>SPBC776.12c |hsk1||serine/threonine protein kinase
           Hsk1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 507

 Score = 24.6 bits (51), Expect = 8.9
 Identities = 18/65 (27%), Positives = 28/65 (43%), Gaps = 1/65 (1%)
 Frame = +2

Query: 137 CCRFLGRRFCTSIAISTTV-VNLRQLPR*RSCSLSSTHRHLMKHTVEVETGTLT*FLSF* 313
           C    G  F T+++  T   VN R+L    SC  +S ++  ++H    E      FL   
Sbjct: 354 CAALHGCTFETNVSTLTEKRVNFRKLILWASCGSASIYKEKLRHKPSQEERLCLDFLEKC 413

Query: 314 WLMDC 328
             +DC
Sbjct: 414 LELDC 418


>SPAC1F7.05 |cdc22||ribonucleoside reductase large subunit
           Cdc22|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 811

 Score = 24.6 bits (51), Expect = 8.9
 Identities = 15/50 (30%), Positives = 25/50 (50%)
 Frame = -2

Query: 383 MDLNSKYRVTPV*ISNLTSNPLAIRNLGIKSTFQSRPRPYVSSGAGALNL 234
           +D+N  Y V     SN+   P+ +   G+   F +   P+ S+GA  LN+
Sbjct: 480 IDVNY-YPVPEARRSNMRHRPVGLGVQGLADAFFALRLPFESAGAKKLNI 528


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,191,921
Number of Sequences: 5004
Number of extensions: 46380
Number of successful extensions: 154
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 153
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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