BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30430 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC22H10.12c |gdi1|sec19|GDP dissociation inhibitor Gdi1 |Schiz... 193 1e-50 SPBC15C4.03 |||Rab geranylgeranyltransferase escort protein |Sch... 51 1e-07 SPBC713.12 |erg1||squalene monooxygenase Erg1 |Schizosaccharomyc... 33 0.033 SPAC12G12.13c |cid14||poly|Schizosaccharomyces pombe|chr 1|||Manual 27 1.3 SPBC2G2.11 |||N-myristoyltransferase 1|Schizosaccharomyces pombe... 27 2.2 SPAC17H9.03c |rdl1||RAD51D-like protein 1|Schizosaccharomyces po... 26 2.9 SPAPB17E12.08 |||N-glycosylation protein |Schizosaccharomyces po... 26 3.8 SPCC777.14 |prp4||serine/threonine protein kinase Prp4|Schizosac... 26 3.8 SPAC17A2.05 |||fumerate reductase|Schizosaccharomyces pombe|chr ... 25 5.1 SPAC139.04c |fap2||L-saccharopine oxidase|Schizosaccharomyces po... 25 6.7 SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|c... 25 6.7 SPAC3F10.16c |||GTP binding protein, HSR1-related|Schizosaccharo... 25 6.7 SPAC8E11.05c |||conserved fungal protein|Schizosaccharomyces pom... 25 8.9 SPBC776.12c |hsk1||serine/threonine protein kinase Hsk1|Schizosa... 25 8.9 SPAC1F7.05 |cdc22||ribonucleoside reductase large subunit Cdc22|... 25 8.9 >SPAC22H10.12c |gdi1|sec19|GDP dissociation inhibitor Gdi1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 440 Score = 193 bits (471), Expect = 1e-50 Identities = 95/149 (63%), Positives = 115/149 (77%), Gaps = 1/149 (0%) Frame = +1 Query: 73 MDEEYDVIVLGTGLKECILSGMLSVSGKKVLHIDRNKYYGGESASITPLEELFAKFN-AP 249 MDEEYDVIVLGTGL EC+LSG+LSV GKKVLHIDRN YYG +SAS+ L +L+A F Sbjct: 1 MDEEYDVIVLGTGLTECVLSGLLSVDGKKVLHIDRNDYYGADSASLN-LTQLYALFRPGE 59 Query: 250 APDETYGRGRDWNVDLIPKFLMANGLLVKLLIHTGVTRYLEFKSIEGSYVYKGGKISKVP 429 E+ GR RDW VDL+PKFLMANG L +LI+T VTRY+EFK I GSYVY+ G+I+KVP Sbjct: 60 QRPESLGRDRDWCVDLVPKFLMANGDLTNILIYTDVTRYIEFKQIAGSYVYRDGRIAKVP 119 Query: 430 VDQKEALASDLMGMFEKRRFRNFLIYVQD 516 ++ EAL S LM +FEKRR + FL +V + Sbjct: 120 GNEMEALKSPLMSLFEKRRAKKFLEWVNN 148 >SPBC15C4.03 |||Rab geranylgeranyltransferase escort protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 459 Score = 50.8 bits (116), Expect = 1e-07 Identities = 48/170 (28%), Positives = 74/170 (43%), Gaps = 26/170 (15%) Frame = +1 Query: 85 YDVIVLGTGLKECILSGMLSVSGKKVLHIDRNKYYGGESASIT--PLEELFAKFNAPAPD 258 YDVI++GT L+ ILS LS + ++VLHID N +YG S+T LE++ K Sbjct: 7 YDVIIVGTNLRNSILSAALSWANQRVLHIDENSFYGEIDGSLTLRDLEQINEKIKKVDSS 66 Query: 259 ETYGRG------------RDWNVDLIPK-----------FLMANGLLVKLLIHTGVTRYL 369 + + N DLIPK + A+ LVKLL T + +YL Sbjct: 67 QILNDNGSHKSPLKRFEVQFLNKDLIPKNKGSVIQFHPQEIFASSELVKLLSETKIYKYL 126 Query: 370 EFKSIEG-SYVYKGGKISKVPVDQKEALASDLMGMFEKRRFRNFLIYVQD 516 K + + KVP + + + + + KR F+ +V + Sbjct: 127 LLKPARSFRLLTSNEEWIKVPESRADIFNNKNLSLASKRIVMRFMKFVSN 176 >SPBC713.12 |erg1||squalene monooxygenase Erg1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 457 Score = 32.7 bits (71), Expect = 0.033 Identities = 12/34 (35%), Positives = 22/34 (64%) Frame = +1 Query: 79 EEYDVIVLGTGLKECILSGMLSVSGKKVLHIDRN 180 ++ D+I++G G+ C L L G+KVL ++R+ Sbjct: 4 QDADIIIIGAGITGCALGAALGRQGRKVLVLERD 37 >SPAC12G12.13c |cid14||poly|Schizosaccharomyces pombe|chr 1|||Manual Length = 684 Score = 27.5 bits (58), Expect = 1.3 Identities = 15/57 (26%), Positives = 27/57 (47%) Frame = +1 Query: 151 GKKVLHIDRNKYYGGESASITPLEELFAKFNAPAPDETYGRGRDWNVDLIPKFLMAN 321 GKK + +RN Y ++ PL K + P+ ++ + +D N D + L+ N Sbjct: 2 GKKSVSFNRNNYKKRKNERTEPLPRRIFKNDKPSKFKSKRKEKDKNSDAYDEMLLNN 58 >SPBC2G2.11 |||N-myristoyltransferase 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 466 Score = 26.6 bits (56), Expect = 2.2 Identities = 12/31 (38%), Positives = 15/31 (48%) Frame = +1 Query: 217 LEELFAKFNAPAPDETYGRGRDWNVDLIPKF 309 L L K A AP +TY + W +PKF Sbjct: 32 LRSLIEKEEAAAPPKTYEDFKFWKTQPVPKF 62 >SPAC17H9.03c |rdl1||RAD51D-like protein 1|Schizosaccharomyces pombe|chr 1|||Manual Length = 230 Score = 26.2 bits (55), Expect = 2.9 Identities = 10/24 (41%), Positives = 18/24 (75%) Frame = +2 Query: 140 CRFLGRRFCTSIAISTTVVNLRQL 211 CR L R+F ++ +ST++V ++QL Sbjct: 116 CRILTRKFRLAVYLSTSLVYIKQL 139 >SPAPB17E12.08 |||N-glycosylation protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 166 Score = 25.8 bits (54), Expect = 3.8 Identities = 13/37 (35%), Positives = 20/37 (54%) Frame = +1 Query: 247 PAPDETYGRGRDWNVDLIPKFLMANGLLVKLLIHTGV 357 P P + G + + V F++ANG L+K LIH + Sbjct: 14 PRPAQALGINQPF-VSAYLSFVLANGFLLKWLIHYSI 49 >SPCC777.14 |prp4||serine/threonine protein kinase Prp4|Schizosaccharomyces pombe|chr 3|||Manual Length = 477 Score = 25.8 bits (54), Expect = 3.8 Identities = 26/86 (30%), Positives = 44/86 (51%), Gaps = 1/86 (1%) Frame = -1 Query: 474 KHAHKIRRKSFFLIDRYFGDFSTFVNITSFDGLKLQVPGDARVNKQFD*QSISHQKLRNQ 295 K +HK+ ++S FL D + DF+ F+ I D + Q + R +F S + +R++ Sbjct: 378 KFSHKMLKRSQFLNDHFDADFN-FIQI-DHDPITNQ---ETRKPVKF---SKPTKDIRSR 429 Query: 294 V-NVPVSTSTVCFIRCRCVELSEQLL 220 + VP ST IR ++L E+ L Sbjct: 430 LKEVPTSTDEEFIIRQELMDLLEKCL 455 >SPAC17A2.05 |||fumerate reductase|Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 25.4 bits (53), Expect = 5.1 Identities = 13/36 (36%), Positives = 19/36 (52%) Frame = +1 Query: 94 IVLGTGLKECILSGMLSVSGKKVLHIDRNKYYGGES 201 IV+G GL + + G VL +D+N +GG S Sbjct: 42 IVIGGGLAGLSATNTILDLGGNVLLLDKNTAFGGNS 77 >SPAC139.04c |fap2||L-saccharopine oxidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 433 Score = 25.0 bits (52), Expect = 6.7 Identities = 11/20 (55%), Positives = 14/20 (70%) Frame = -2 Query: 503 IKKLRKRLFSNMPIRSDARA 444 I+K LFSN P+RSD +A Sbjct: 126 IRKKYPSLFSNSPLRSDMQA 145 >SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 649 Score = 25.0 bits (52), Expect = 6.7 Identities = 11/27 (40%), Positives = 15/27 (55%) Frame = +2 Query: 338 CLFTRASPGTWSLSPSKEVMFTKVEKS 418 C+F R S TWS+ S M ++ KS Sbjct: 487 CVFIRTSMQTWSMFRSTHSMKQEIAKS 513 >SPAC3F10.16c |||GTP binding protein, HSR1-related|Schizosaccharomyces pombe|chr 1|||Manual Length = 616 Score = 25.0 bits (52), Expect = 6.7 Identities = 11/32 (34%), Positives = 16/32 (50%) Frame = +1 Query: 163 LHIDRNKYYGGESASITPLEELFAKFNAPAPD 258 L D N + A++ LE +F KF + PD Sbjct: 266 LQADENDVHSSRIATLKVLEGIFEKFASTLPD 297 >SPAC8E11.05c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 338 Score = 24.6 bits (51), Expect = 8.9 Identities = 12/35 (34%), Positives = 18/35 (51%) Frame = -2 Query: 179 LRSMCKTFFPETDNMPLKIHSFKPVPRTITSYSSS 75 LRS + F T +P+ + P P T S++SS Sbjct: 195 LRSSIRHIFLVTMGLPVDLDELLPTPSTQGSFNSS 229 >SPBC776.12c |hsk1||serine/threonine protein kinase Hsk1|Schizosaccharomyces pombe|chr 2|||Manual Length = 507 Score = 24.6 bits (51), Expect = 8.9 Identities = 18/65 (27%), Positives = 28/65 (43%), Gaps = 1/65 (1%) Frame = +2 Query: 137 CCRFLGRRFCTSIAISTTV-VNLRQLPR*RSCSLSSTHRHLMKHTVEVETGTLT*FLSF* 313 C G F T+++ T VN R+L SC +S ++ ++H E FL Sbjct: 354 CAALHGCTFETNVSTLTEKRVNFRKLILWASCGSASIYKEKLRHKPSQEERLCLDFLEKC 413 Query: 314 WLMDC 328 +DC Sbjct: 414 LELDC 418 >SPAC1F7.05 |cdc22||ribonucleoside reductase large subunit Cdc22|Schizosaccharomyces pombe|chr 1|||Manual Length = 811 Score = 24.6 bits (51), Expect = 8.9 Identities = 15/50 (30%), Positives = 25/50 (50%) Frame = -2 Query: 383 MDLNSKYRVTPV*ISNLTSNPLAIRNLGIKSTFQSRPRPYVSSGAGALNL 234 +D+N Y V SN+ P+ + G+ F + P+ S+GA LN+ Sbjct: 480 IDVNY-YPVPEARRSNMRHRPVGLGVQGLADAFFALRLPFESAGAKKLNI 528 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,191,921 Number of Sequences: 5004 Number of extensions: 46380 Number of successful extensions: 154 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 148 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 153 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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