BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30420 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual 46 3e-06 SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 46 3e-06 SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 36 0.003 SPBP35G2.08c |air1||zinc knuckle TRAMP complex subunit Air1|Schi... 32 0.044 SPAC13D6.02c |byr3||zinc finger protein Byr3|Schizosaccharomyces... 31 0.14 SPAC29B12.12 |||helper of TIM |Schizosaccharomyces pombe|chr 1||... 27 1.3 SPBP35G2.13c |swc2||chromatin remodeling complex subunit Swc2 |S... 26 2.9 SPAC22A12.07c |ogm1|oma1|protein O-mannosyltransferase Ogm1|Schi... 25 6.7 SPAC15A10.04c |zpr1||zinc finger protein Zpr1|Schizosaccharomyce... 25 6.7 SPAC13G6.04 |tim8||TIM22 inner membrane protein import complex s... 25 6.7 SPAC27F1.08 |pdt1||Nramp family manganese ion transporter|Schizo... 25 6.7 >SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual Length = 438 Score = 46.0 bits (104), Expect = 3e-06 Identities = 23/71 (32%), Positives = 32/71 (45%), Gaps = 2/71 (2%) Frame = +3 Query: 84 STMP--FKPADNPKCPKCGKSVYAAEERVAGGLKWHKMCFKCGLCQKLLDSTNCSEHEGE 257 ST P ++ C CG S+ A A G K H CFKC C + L+ EG+ Sbjct: 244 STKPVLYRGNSEKSCHSCGGSLRAGRIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGK 303 Query: 258 LYCKVCHARKF 290 YC + + +F Sbjct: 304 FYCHLDYHEQF 314 Score = 37.1 bits (82), Expect = 0.002 Identities = 17/55 (30%), Positives = 24/55 (43%) Frame = +3 Query: 117 KCPKCGKSVYAAEERVAGGLKWHKMCFKCGLCQKLLDSTNCSEHEGELYCKVCHA 281 KC KC K + + + G ++H C+ CG C LL E C+ C A Sbjct: 377 KCKKCRKPILGISVKGSDG-EYHSQCWTCGACNALLGDEGYFMIENTPICRPCKA 430 >SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosaccharomyces pombe|chr 2|||Manual Length = 933 Score = 46.0 bits (104), Expect = 3e-06 Identities = 20/55 (36%), Positives = 31/55 (56%), Gaps = 3/55 (5%) Frame = +3 Query: 120 CPKCGKSVYAAEERVAGGLKWHKMCFKCGLCQKLLDSTN---CSEHEGELYCKVC 275 C KC +SV + + GG WH CFKC C K LD ++ + + +++CK+C Sbjct: 24 CIKCWESVPSTSQVWFGGKCWHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCKLC 78 Score = 24.6 bits (51), Expect = 8.9 Identities = 10/36 (27%), Positives = 19/36 (52%) Frame = -3 Query: 283 RAWHTLQYSSPSCSEQLVESSNFWHRPHLKHILCHL 176 + WH+ + +C+++L SS + + K I C L Sbjct: 42 KCWHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCKL 77 >SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharomyces pombe|chr 1|||Manual Length = 969 Score = 36.3 bits (80), Expect = 0.003 Identities = 18/56 (32%), Positives = 27/56 (48%), Gaps = 4/56 (7%) Frame = +3 Query: 120 CPKCGKSVYAAEERVA-GGLKWHKMCFKCGLCQKLLDSTN---CSEHEGELYCKVC 275 C +CG++ E ++ GG WHK CF C C K L+ ++ +G C C Sbjct: 19 CFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSSC 74 Score = 32.7 bits (71), Expect = 0.033 Identities = 15/60 (25%), Positives = 26/60 (43%), Gaps = 6/60 (10%) Frame = +3 Query: 114 PKCPKCGKSVYAAEERV------AGGLKWHKMCFKCGLCQKLLDSTNCSEHEGELYCKVC 275 P C C + A R+ +G +H+ CF+C C+K + +N ++C C Sbjct: 69 PVCSSCAHTCTACRMRIKDYALMSGYDSYHRECFRCHDCRKQIIDSNFKRDNRTIFCNDC 128 >SPBP35G2.08c |air1||zinc knuckle TRAMP complex subunit Air1|Schizosaccharomyces pombe|chr 2|||Manual Length = 313 Score = 32.3 bits (70), Expect = 0.044 Identities = 17/55 (30%), Positives = 24/55 (43%), Gaps = 2/55 (3%) Frame = +3 Query: 120 CPKCGKSVYAAEERVAGGLKWHKMCFKCGLCQKLLDSTNCSE--HEGELYCKVCH 278 C CG A ++ ++ W K C CGL + + CSE G C+ CH Sbjct: 107 CTTCG----AIDDHISVRCPWTKKCMNCGLLGHI--AARCSEPRKRGPRVCRTCH 155 >SPAC13D6.02c |byr3||zinc finger protein Byr3|Schizosaccharomyces pombe|chr 1|||Manual Length = 179 Score = 30.7 bits (66), Expect = 0.14 Identities = 17/63 (26%), Positives = 28/63 (44%) Frame = +3 Query: 87 TMPFKPADNPKCPKCGKSVYAAEERVAGGLKWHKMCFKCGLCQKLLDSTNCSEHEGELYC 266 T+P P+C CG++ + A E G + C+ C Q ++ C+E + E C Sbjct: 8 TVPQTTRPGPRCYNCGENGHQARECTKGSI-----CYNCN--QTGHKASECTEPQQEKTC 60 Query: 267 KVC 275 C Sbjct: 61 YAC 63 >SPAC29B12.12 |||helper of TIM |Schizosaccharomyces pombe|chr 1|||Manual Length = 113 Score = 27.5 bits (58), Expect = 1.3 Identities = 11/34 (32%), Positives = 18/34 (52%) Frame = +3 Query: 177 KWHKMCFKCGLCQKLLDSTNCSEHEGELYCKVCH 278 K+H C CG C+ +S E+ ++CK C+ Sbjct: 65 KFHIPCVICGACK---NSLTVEEYRSTVHCKYCN 95 >SPBP35G2.13c |swc2||chromatin remodeling complex subunit Swc2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 316 Score = 26.2 bits (55), Expect = 2.9 Identities = 11/21 (52%), Positives = 15/21 (71%) Frame = -3 Query: 229 ESSNFWHRPHLKHILCHLRPP 167 E+SN++ P L+H LCH PP Sbjct: 241 EASNYYVAP-LEHPLCHSAPP 260 >SPAC22A12.07c |ogm1|oma1|protein O-mannosyltransferase Ogm1|Schizosaccharomyces pombe|chr 1|||Manual Length = 893 Score = 25.0 bits (52), Expect = 6.7 Identities = 12/35 (34%), Positives = 17/35 (48%) Frame = +3 Query: 138 SVYAAEERVAGGLKWHKMCFKCGLCQKLLDSTNCS 242 SVY A+++ L+WH G+ L ST S Sbjct: 189 SVYEAQQKRPFSLRWHTSLLSTGVALGLALSTKLS 223 >SPAC15A10.04c |zpr1||zinc finger protein Zpr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 459 Score = 25.0 bits (52), Expect = 6.7 Identities = 10/30 (33%), Positives = 13/30 (43%) Frame = -3 Query: 274 HTLQYSSPSCSEQLVESSNFWHRPHLKHIL 185 HT + PSCS Q PH K ++ Sbjct: 253 HTFHATCPSCSHQCDTHMKLLDIPHFKEVI 282 >SPAC13G6.04 |tim8||TIM22 inner membrane protein import complex subunit Tim8|Schizosaccharomyces pombe|chr 1|||Manual Length = 98 Score = 25.0 bits (52), Expect = 6.7 Identities = 8/23 (34%), Positives = 14/23 (60%) Frame = -3 Query: 469 KVQYGVRSRSTTGWPRCVSAIRN 401 K+Q + ++T WP+C+ I N Sbjct: 32 KLQQAIHQFTSTCWPKCIGNIGN 54 >SPAC27F1.08 |pdt1||Nramp family manganese ion transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 521 Score = 25.0 bits (52), Expect = 6.7 Identities = 7/23 (30%), Positives = 14/23 (60%) Frame = -3 Query: 463 QYGVRSRSTTGWPRCVSAIRNFC 395 +YG+++ + W +C IR +C Sbjct: 50 KYGIQTGFSKYWKKCTYGIREYC 72 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,949,351 Number of Sequences: 5004 Number of extensions: 34322 Number of successful extensions: 91 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 82 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 91 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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