BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30406 (516 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 306 1e-84 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 162 3e-41 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 153 2e-38 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 110 1e-25 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 82 6e-17 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 79 4e-16 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 69 3e-13 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 58 6e-10 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 40 3e-04 SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 27 1.7 SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosacc... 25 5.1 SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces... 25 6.7 SPAC1142.03c |swi2|SPAC17G6.20c|Swi5 complex subunit Swi2|Schizo... 25 8.9 SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo... 25 8.9 SPBC18E5.09c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 25 8.9 SPAC23H4.13c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 25 8.9 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 306 bits (751), Expect = 1e-84 Identities = 143/163 (87%), Positives = 153/163 (93%) Frame = +2 Query: 26 RGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERF 205 RGY+F+TTAEREIVRDIKEKLCYVALDFEQE+ TAA S+SLEKSYELPDGQVITIGNERF Sbjct: 196 RGYTFSTTAEREIVRDIKEKLCYVALDFEQELQTAAQSSSLEKSYELPDGQVITIGNERF 255 Query: 206 RCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQK 385 R PEALFQPS LG+E+ GIHE YNSIMKCDVDIRKDLY N VMSGGTTMYPGIADRMQK Sbjct: 256 RAPEALFQPSALGLENAGIHEATYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIADRMQK 315 Query: 386 EITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWIS 514 EI ALAPS++K+KI+APPERKYSVWIGGSILASLSTFQQMWIS Sbjct: 316 EIQALAPSSMKVKIVAPPERKYSVWIGGSILASLSTFQQMWIS 358 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 162 bits (393), Expect = 3e-41 Identities = 73/162 (45%), Positives = 108/162 (66%) Frame = +2 Query: 29 GYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFR 208 G+ ++AE+EIVR+IKEK CYVA DF E+ + +Y+LPD Q IT+G E F Sbjct: 202 GHELVSSAEKEIVREIKEKCCYVASDFRSEIESWTEHKPQIHTYQLPDNQTITLGTECFS 261 Query: 209 CPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKE 388 PE LF P +G E+ G+H ++ SI+ D+D+R LY+N V+SGG+T+ G +R E Sbjct: 262 APEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGERFISE 321 Query: 389 ITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWIS 514 + A++ ++KI A PER ++ W+GGSILASLSTF+++ I+ Sbjct: 322 LRAISGKKNQVKIYASPERMHNAWLGGSILASLSTFRRLLIT 363 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 153 bits (370), Expect = 2e-38 Identities = 76/175 (43%), Positives = 115/175 (65%), Gaps = 12/175 (6%) Frame = +2 Query: 26 RGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERF 205 +GY+F TA+ E VR++KEKLCYV+ D E + + +T L ++Y LPDG+VI +G+ER+ Sbjct: 196 KGYAFNRTADFETVREMKEKLCYVSYDLELDHKLSEETTVLMRNYTLPDGRVIKVGSERY 255 Query: 206 RCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQK 385 CPE LFQP +G E G+ E ++++I DVDIRK LY V+SGG++MY G+ R++K Sbjct: 256 ECPECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPSRLEK 315 Query: 386 EITAL--------APSTI---KIKIIAPPERKYSVWIGGSILAS-LSTFQQMWIS 514 EI L P+ + K+KI P R+++V+IGG++LA ++ MW+S Sbjct: 316 EIKQLWFERVLHGDPARLPNFKVKIEDAPRRRHAVFIGGAVLADIMAQNDHMWVS 370 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 110 bits (264), Expect = 1e-25 Identities = 64/171 (37%), Positives = 90/171 (52%), Gaps = 11/171 (6%) Frame = +2 Query: 35 SFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCP 214 S+ +R ++ + KE + V + A AS+ K +E PDG G ERFR Sbjct: 243 SYEIFQKRRVIEEWKESVLDV---LDTPFDEAKASSRNPKPFEFPDGVTHKFGQERFRIS 299 Query: 215 EALFQPSFLGMESC---------GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGI 367 E LF PSF S G+HE VY SI+ CD ++R L N V++GGT++ PG+ Sbjct: 300 EILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSELRSPLLNNIVVTGGTSLIPGL 359 Query: 368 ADRMQKEITALAP-STIKIKII-APPERKYSVWIGGSILASLSTFQQMWIS 514 ++R+Q E+ LA S I + +VW GGSILASL FQ +W+S Sbjct: 360 SERLQAEVQRLATGSRINVHTAETASATSNAVWFGGSILASLDNFQHLWVS 410 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 81.8 bits (193), Expect = 6e-17 Identities = 41/89 (46%), Positives = 58/89 (65%), Gaps = 3/89 (3%) Frame = +2 Query: 257 GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAP 436 GI + N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P + ++KI A Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHAS 384 Query: 437 ---PERKYSVWIGGSILASLSTFQQMWIS 514 ER Y+ W+GGSIL+SL TF Q+WIS Sbjct: 385 GHVVERSYASWLGGSILSSLGTFHQLWIS 413 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 79.0 bits (186), Expect = 4e-16 Identities = 49/150 (32%), Positives = 73/150 (48%), Gaps = 20/150 (13%) Frame = +2 Query: 53 EREIVRDIKEKLCYVALDFEQEMATAAAS--TSLEKSYELPD------------------ 172 E IV +IKE +CYV+ +F+++M + LE Y LPD Sbjct: 211 ETYIVNEIKESVCYVSQNFKEDMEICHEKPRSKLEICYALPDYSTGKHGYIVRDINQKIE 270 Query: 173 GQVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTT 352 QV+ + NERF PE LF PS + + GI E V S+ +I+ L N V GG Sbjct: 271 QQVLNLSNERFMIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNC 330 Query: 353 MYPGIADRMQKEITALAPSTIKIKIIAPPE 442 +PG R+ E+ +LAP+ ++K+ P + Sbjct: 331 KFPGFHKRLSSELRSLAPANWEVKVFEPSD 360 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 69.3 bits (162), Expect = 3e-13 Identities = 47/160 (29%), Positives = 74/160 (46%), Gaps = 19/160 (11%) Frame = +2 Query: 74 IKEKLCYVALDFEQEMATAAASTS--LEKSYELPDGQVITI--GNERFRCPEALFQPSFL 241 IKE+ CYV D +E + L+ + E G TI G ERF PE F P Sbjct: 240 IKEECCYVCPDIVKEFSRFDREPDRYLKYASESITGHSTTIDVGFERFLAPEIFFNPEIA 299 Query: 242 GMESCG-IHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTI- 415 + + E V N + +D+RK LY N V+SGG+T++ +R+Q+++ + I Sbjct: 300 SSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGNRLQRDLKRIVDERIH 359 Query: 416 -------------KIKIIAPPERKYSVWIGGSILASLSTF 496 + +I+ ++ +VW GGS+LA F Sbjct: 360 RSEMLSGAKSGGVDVNVISHKRQRNAVWFGGSLLAQTPEF 399 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 58.4 bits (135), Expect = 6e-10 Identities = 25/92 (27%), Positives = 50/92 (54%) Frame = +2 Query: 197 ERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADR 376 E+ R PE +F PS +G++ GI E + + + + ++ ++ L +N +++GG PG+ R Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653 Query: 377 MQKEITALAPSTIKIKIIAPPERKYSVWIGGS 472 +++E+T++ P I + W G S Sbjct: 654 IKRELTSIMPVGSSINVFRASNPLLDAWKGAS 685 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 39.5 bits (88), Expect = 3e-04 Identities = 18/70 (25%), Positives = 36/70 (51%), Gaps = 3/70 (4%) Frame = +2 Query: 311 KDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPP---ERKYSVWIGGSILA 481 K+LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586 Query: 482 SLSTFQQMWI 511 + ++WI Sbjct: 587 RIRIVSELWI 596 >SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 617 Score = 27.1 bits (57), Expect = 1.7 Identities = 14/37 (37%), Positives = 20/37 (54%) Frame = +2 Query: 383 KEITALAPSTIKIKIIAPPERKYSVWIGGSILASLST 493 KEI++ A S I + + P E+ W ILA L+T Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248 >SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 284 Score = 25.4 bits (53), Expect = 5.1 Identities = 15/43 (34%), Positives = 20/43 (46%) Frame = +1 Query: 262 PRDRVQLHHEVRRRHP*GPVRQHRHVRWYHHVPRYRRQDAEGD 390 PR+ V R+R P P + R V PR RRQ +G+ Sbjct: 41 PRELVAQSTTSRKRDPNQPTPRERTVNKKADQPRRRRQAPQGN 83 >SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces pombe|chr 3|||Manual Length = 879 Score = 25.0 bits (52), Expect = 6.7 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = +1 Query: 409 DHQDQDHRSPREEVLRMDRWIHPG 480 D D+ R+ E VLRM R++ PG Sbjct: 499 DIDDKKDRALSEHVLRMHRYLPPG 522 >SPAC1142.03c |swi2|SPAC17G6.20c|Swi5 complex subunit Swi2|Schizosaccharomyces pombe|chr 1|||Manual Length = 722 Score = 24.6 bits (51), Expect = 8.9 Identities = 22/93 (23%), Positives = 37/93 (39%), Gaps = 1/93 (1%) Frame = +2 Query: 179 VITIGNERFRCPEALFQPSFLGMESC-GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTM 355 ++T+ NE + E L S S E+ + CD+D ++ + + T + Sbjct: 136 LVTLPNENNQVIEPLSSQSCKSQLSTQNYSESDFGWNQLCDLD---PIFKSLAFTDDTNL 192 Query: 356 YPGIADRMQKEITALAPSTIKIKIIAPPERKYS 454 +P AD I ++K A RKYS Sbjct: 193 FPAFADSEAALIMLKKREMTRVKHRAGRPRKYS 225 >SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 689 Score = 24.6 bits (51), Expect = 8.9 Identities = 12/32 (37%), Positives = 17/32 (53%) Frame = -1 Query: 450 YFLSGGAMILILMVEGARAVISFCILSAIPGY 355 Y +SGGA + +A +S CI +PGY Sbjct: 412 YCISGGAAL----AASTQAFLSSCICPVLPGY 439 >SPBC18E5.09c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 128 Score = 24.6 bits (51), Expect = 8.9 Identities = 13/43 (30%), Positives = 21/43 (48%), Gaps = 1/43 (2%) Frame = +2 Query: 89 CYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNE-RFRCP 214 CYV+ +E + + SL SY +PD + I + R+ P Sbjct: 39 CYVSRPLTKERSCSHPYISLGSSYGIPDAENIEYPRDARYHSP 81 >SPAC23H4.13c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual Length = 184 Score = 24.6 bits (51), Expect = 8.9 Identities = 16/36 (44%), Positives = 19/36 (52%) Frame = +2 Query: 74 IKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQV 181 IKEKL VA EQE A +SL+ S + G V Sbjct: 111 IKEKLRRVANITEQEQQCIPAESSLDLSIQAMKGVV 146 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,904,843 Number of Sequences: 5004 Number of extensions: 34153 Number of successful extensions: 124 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 114 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 118 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 208287218 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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