Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30398
(516 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 71 1e-13
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 71 1e-13
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 54 1e-08
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 31 0.10
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 26 3.8
SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces pombe... 25 5.1
SPCC895.08c |||conserved fungal protein|Schizosaccharomyces pomb... 25 6.7
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 25 6.7
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 70.9 bits (166), Expect = 1e-13
Identities = 44/136 (32%), Positives = 71/136 (52%)
Frame = +1
Query: 43 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXXFLPIYSQA 222
AFS++D G G+I ++GDLLRA NPTLA I FL + ++
Sbjct: 11 AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI---TEIESTLPAEVDMEQFLQVLNRP 67
Query: 223 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPED 402
G E+F++ +++DK+ G++ EL + L +LGEKL + E+ E+ K
Sbjct: 68 NGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PV 124
Query: 403 DDGMIPYAAFLKKVMA 450
DGM+ Y F++ ++A
Sbjct: 125 KDGMVNYHDFVQMILA 140
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 70.5 bits (165), Expect = 1e-13
Identities = 47/149 (31%), Positives = 74/149 (49%), Gaps = 3/149 (2%)
Frame = +1
Query: 7 TSAXNDVERASF--AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXX 180
T D + A F AFS++D + G I + LG ++R+L +PT A +
Sbjct: 3 TRNLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGN 62
Query: 181 XXXXXX-FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDD 357
FL + ++ KD D E+ E K++DK+ NG + ELTH L +LGE+L
Sbjct: 63 GTIDFTEFLTMMARKMKDTDNE--EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQ 120
Query: 358 SEVAEVTKDCMDPEDDDGMIPYAAFLKKV 444
EVA++ ++ D DG+I Y F + +
Sbjct: 121 EEVADMIREA--DTDGDGVINYEEFSRVI 147
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 54.4 bits (125), Expect = 1e-08
Identities = 35/136 (25%), Positives = 68/136 (50%)
Frame = +1
Query: 43 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXXFLPIYSQA 222
AF +YD + G I ++G +LR+L N T A + F+ +
Sbjct: 14 AFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFV-----S 68
Query: 223 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPED 402
K ++ + E++++ +++DK+ +G + A+ + LGEKL D+EV + ++ DP
Sbjct: 69 NKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEA-DP-T 126
Query: 403 DDGMIPYAAFLKKVMA 450
+ G Y F++++MA
Sbjct: 127 NSGSFDYYDFVQRIMA 142
Score = 30.7 bits (66), Expect = 0.14
Identities = 14/60 (23%), Positives = 34/60 (56%)
Frame = +1
Query: 250 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 429
++ E LYD +++GL+ + + L +LG + D+E+A+++ + D D+ + + +
Sbjct: 9 DEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFVS 68
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 31.1 bits (67), Expect = 0.10
Identities = 29/136 (21%), Positives = 53/136 (38%), Gaps = 1/136 (0%)
Frame = +1
Query: 43 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXX-FLPIYSQ 219
AF ++D + ID L +RAL N + + F+ + ++
Sbjct: 42 AFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMTE 101
Query: 220 AKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPE 399
++D E+ +L+D +E G + L L E +DD E+ + ++
Sbjct: 102 KIVERDP--LEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEF--DL 157
Query: 400 DDDGMIPYAAFLKKVM 447
D DG I F+ +M
Sbjct: 158 DQDGEINEQEFIAIMM 173
Score = 29.5 bits (63), Expect = 0.31
Identities = 15/58 (25%), Positives = 30/58 (51%)
Frame = +1
Query: 211 YSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKD 384
Y+ + + + +D E KL+D +++ + EL + ALG + SEV ++ +D
Sbjct: 24 YAPLRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRD 81
Score = 25.4 bits (53), Expect = 5.1
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +1
Query: 25 VERASFAFSIYDFEGKGKIDAFNLGDLLRALNSN 126
+E AF ++D + GKI NL + + LN N
Sbjct: 109 LEEIKRAFELFDDDETGKISLRNLRRVAKELNEN 142
>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1142
Score = 25.8 bits (54), Expect = 3.8
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = +1
Query: 337 LGEKLDDSEVAEVTKDCMDPED 402
L EK+ D + + DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798
>SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 551
Score = 25.4 bits (53), Expect = 5.1
Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 2/75 (2%)
Frame = +2
Query: 20 TTLKGRLSPSQSTTLKA-KVRSMPS-TLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSHS 193
T +PS L+A + S+P+ +L S E + + +R ++ ++ S +
Sbjct: 309 TEYSSNTAPSNWIALEALPLVSIPNESLDESDELAESLSDSEAARLQLLGIKKQESVAKK 368
Query: 194 KSSFPSTAKQRKTKT 238
KSSFPST K + T
Sbjct: 369 KSSFPSTIKDQPNLT 383
>SPCC895.08c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 490
Score = 25.0 bits (52), Expect = 6.7
Identities = 19/78 (24%), Positives = 38/78 (48%)
Frame = +2
Query: 11 QQXTTLKGRLSPSQSTTLKAKVRSMPSTLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSH 190
++ ++L +L+P T KAK S+ S S S+ + W + V++ A S
Sbjct: 345 REPSSLSRKLAPPFLRTHKAKSNSLFSMKRPSSSSSSLSGSWHGDTENSVKQSLA---SP 401
Query: 191 SKSSFPSTAKQRKTKTRE 244
S++S P+ +K + ++
Sbjct: 402 SEASLPNLSKYSRKNAKK 419
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 25.0 bits (52), Expect = 6.7
Identities = 12/22 (54%), Positives = 14/22 (63%), Gaps = 1/22 (4%)
Frame = +1
Query: 22 DVER-ASFAFSIYDFEGKGKID 84
DV R SF F +YDF G G +D
Sbjct: 612 DVMRNISFIFELYDFNGDGFMD 633
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,876,409
Number of Sequences: 5004
Number of extensions: 34076
Number of successful extensions: 115
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 106
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 111
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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